BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	143,596	G→A	5.3%	G16S (GGC→AGC)	PP_0136 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	143,596	0	G	A	5.3%	979.4 / 27.6	301	G16S (GGC→AGC)	PP_0136	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (159/126); new base A (7/9); total (166/135)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 316 total aligned reads displayed.

GTGGTATCGCGGGCGCTGCTACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATCTGACCGGCAGT  >  NC_002947/143448‑143745

gtggtATCGCGGGCGCTGCTACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTggg                                                                                                                                                      <  2:3059752/150‑1 (MQ=255)

 tggtATCGCGGGCGCTGCTACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGggc                                                                                                                                                     <  2:2177825/150‑1 (MQ=255)

 tggtATCGCGGGCGCTGCTACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGggc                                                                                                                                                     <  2:4177225/150‑1 (MQ=255)

   gtATCGCGGGCGCTGCTACAGGTATCGACAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTCTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGggcgg                                                                                                                                                   <  2:5419706/150‑1 (MQ=255)

    tATCGCGGGCGCTGCTACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGggcggc                                                                                                                                                  <  2:6063472/150‑1 (MQ=255)

            gcgcTGCTACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCGCTGCCGCCCAGCAGCAGggcggc                                                                                                                                          <  1:4877565/150‑1 (MQ=255)

             cgcTGCTACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCgcggcg                                                                                                                                         >  1:2273519/1‑150 (MQ=255)

              gctgctACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGcggcgg                                                                                                                                        >  1:6213663/1‑150 (MQ=255)

                tgctACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGcc                                                                                                                                      >  1:7328469/1‑150 (MQ=255)

                   tACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATg                                                                                                                                   >  2:7329238/1‑150 (MQ=255)

                    aCAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATgg                                                                                                                                  >  1:5495285/1‑150 (MQ=255)

                     cAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATggc                                                                                                                                 <  1:4952460/150‑1 (MQ=255)

                      aGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGCCGCCGCGCTGCCGCCCAGCAGCAGGGCGGCGACCACggct                                                                                                                                <  2:5209788/150‑2 (MQ=37)

                         gaTCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGcc                                                                                                                             <  2:3211084/149‑1 (MQ=255)

                             gCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATg                                                                                                                         >  2:491463/1‑150 (MQ=255)

                              ccAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGa                                                                                                                        >  2:4639555/1‑150 (MQ=255)

                               cAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGAc                                                                                                                       >  1:3157363/1‑150 (MQ=255)

                                 aGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACgg                                                                                                                     >  1:1641980/1‑150 (MQ=255)

                                  gATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGc                                                                                                                    >  2:6912634/1‑150 (MQ=255)

                                   aTCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGcc                                                                                                                   >  2:6451114/1‑150 (MQ=255)

                                      cTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTATTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAgg                                                                                                                >  2:6297343/1‑150 (MQ=255)

                                       tGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGt                                                                                                               >  2:7247283/1‑150 (MQ=255)

                                        gTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGtt                                                                                                              >  1:4191932/1‑150 (MQ=255)

                                           cATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCgg                                                                                                           <  1:3331724/150‑1 (MQ=255)

                                            aTTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCggg                                                                                                          >  2:586940/1‑150 (MQ=255)

                                            aTTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCggg                                                                                                          >  2:5133366/1‑150 (MQ=255)

                                             ttGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGt                                                                                                         <  1:4428406/150‑1 (MQ=255)

                                             ttGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGt                                                                                                         >  1:1944164/1‑150 (MQ=255)

                                             ttGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGATGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGt                                                                                                         <  1:1656797/150‑1 (MQ=255)

                                              tGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTc                                                                                                        <  1:6244324/150‑1 (MQ=255)

                                              tGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTc                                                                                                        >  1:6088178/1‑150 (MQ=255)

                                                  ttCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATggc                                                                                                                                 <  1:986996/121‑1 (MQ=255)

                                                  ttCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGAcc                                                                                                    >  1:4191211/1‑150 (MQ=255)

                                                   tCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCa                                                                                                   >  2:2805895/1‑150 (MQ=255)

                                                   tCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCa                                                                                                   >  2:3511865/1‑150 (MQ=255)

                                                      tGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACt                                                                                                >  1:1154632/1‑150 (MQ=255)

                                                      tGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACt                                                                                                >  1:2965473/1‑150 (MQ=255)

                                                      tGGTGATGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACt                                                                                                >  2:5313546/1‑150 (MQ=255)

                                                        gtgtTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTgc                                                                                              <  1:6508103/150‑1 (MQ=255)

                                                        gtgtTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTgc                                                                                              >  1:6885725/1‑150 (MQ=255)

                                                           ttgtttgtAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTgctcg                                                                                           >  1:1022575/1‑150 (MQ=255)

                                                           ttgtttgtAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTgctcg                                                                                           >  2:4207547/1‑150 (MQ=255)

                                                                   aGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGAc                                                                                   <  1:3423646/150‑1 (MQ=255)

                                                                    gCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGAcc                                                                                  >  2:3861234/1‑150 (MQ=255)

                                                                      ctcctTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAATCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCg                                                                                >  2:3291334/1‑150 (MQ=255)

                                                                      ctcctTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCg                                                                                <  1:586940/150‑1 (MQ=255)

                                                                      ctcctTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCg                                                                                >  2:6800865/1‑150 (MQ=255)

                                                                      ctcctTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCg                                                                                >  1:6616905/1‑150 (MQ=255)

                                                                        cctTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGa                                                                              <  2:5923669/150‑1 (MQ=255)

                                                                          ttcttcttTGGACGCGGACGAGGTTGAAGATGAGCAGGAGGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGt                                                                            >  1:7122579/1‑150 (MQ=255)

                                                                          ttcttcttTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGt                                                                            >  1:93427/1‑150 (MQ=255)

                                                                           tcttcttTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTa                                                                           >  1:3001284/1‑150 (MQ=255)

                                                                           tcttcttTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTa                                                                           >  1:6746233/1‑150 (MQ=255)

                                                                             ttcttTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTAcc                                                                         >  1:4656335/1‑150 (MQ=255)

                                                                               cttTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCgg                                                                       <  2:5007833/150‑1 (MQ=255)

                                                                                    gACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAAc                                                                  <  1:6451114/150‑1 (MQ=255)

                                                                                    gACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAAc                                                                  >  2:1745210/1‑150 (MQ=255)

                                                                                     aCGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAAcc                                                                 >  1:700354/1‑150 (MQ=255)

                                                                                      cgcgGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCt                                                                <  1:3719704/150‑1 (MQ=255)

                                                                                       gcgGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTg                                                               >  2:6981074/1‑150 (MQ=255)

                                                                                        cgGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTgg                                                              <  2:828600/150‑1 (MQ=255)

                                                                                             gAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGa                                                         >  1:1975541/1‑150 (MQ=255)

                                                                                                gTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGacac                                                      >  1:148241/1‑150 (MQ=255)

                                                                                                 ttGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACAcc                                                     <  1:2716742/150‑1 (MQ=255)

                                                                                                 ttGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGAAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACAcc                                                     >  2:1739701/1‑150 (MQ=255)

                                                                                                  tGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTC‑GACCCGGAGTACCGGGAAACCTGGCAGGACAccca                                                   >  1:5987247/1‑149 (MQ=255)

                                                                                                   gAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCt                                                   >  2:3387714/1‑150 (MQ=255)

                                                                                                    aaGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCACTGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTc                                                  <  2:2552570/150‑1 (MQ=255)

                                                                                                     aGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCg                                                 >  2:5351418/1‑150 (MQ=255)

                                                                                                      gATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGa                                                >  1:5605851/1‑150 (MQ=255)

                                                                                                           gCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCg                                           >  1:5081470/1‑150 (MQ=255)

                                                                                                            cAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGa                                          <  2:1134286/150‑1 (MQ=255)

                                                                                                            cAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGa                                          <  1:2509319/150‑1 (MQ=255)

                                                                                                            cAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGa                                          <  1:201632/150‑1 (MQ=255)

                                                                                                            cAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGa                                          >  1:6276108/1‑150 (MQ=255)

                                                                                                            cAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGa                                          <  2:2408769/150‑1 (MQ=255)

                                                                                                            cAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGa                                          <  1:4733802/150‑1 (MQ=255)

                                                                                                             aGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGTGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAg                                         >  1:2076702/1‑150 (MQ=255)

                                                                                                             aGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAg                                         <  2:4448629/150‑1 (MQ=255)

                                                                                                             aGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAg                                         >  1:3886648/1‑150 (MQ=255)

                                                                                                               gAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGa                                       >  2:562865/1‑150 (MQ=255)

                                                                                                                    ttCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGcc                                  >  2:1369130/1‑150 (MQ=255)

                                                                                                                         gccgCCATGGCCGCCGCGCTGCCGCCCAGCAGCAGGGCGGCGACCACGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGaa                                               <  2:6607263/129‑1 (MQ=21)

                                                                                                                             ccGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGAtt                         <  2:365913/150‑1 (MQ=255)

                                                                                                                                tGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTggg                      <  1:3808430/150‑1 (MQ=255)

                                                                                                                                tGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTggg                      <  2:1944164/150‑1 (MQ=255)

                                                                                                                                tGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTggg                      <  1:4836748/150‑1 (MQ=255)

                                                                                                                                  gTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGACGGCGAGGAACGCCTGCCCGATTGggtg                    >  2:1245871/1‑150 (MQ=255)

                                                                                                                                  gTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGggtg                    <  2:2017050/150‑1 (MQ=255)

                                                                                                                                  gTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGggtg                    <  1:1291513/150‑1 (MQ=255)

                                                                                                                                   ccgccgcGCTGCCGCCCAGCAGCAGGGCGGCGACCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGggtgg                   >  2:2714634/2‑150 (MQ=38)

                                                                                                                                    tgccgccCAGCAGCCGGGCGGCCGCGCGGCGGCCCGGGCGGCCAATGCCGGCCAGGTTCGGGTCGCCCAACTGCTCGGCTCGGACCCGGAGGACCGGGAAACCTGGCAGGCCACCCTCGAAGGCGAGGACCGCCTGCCCGAGGGggtggg                  >  2:233433/2‑149 (MQ=255)

                                                                                                                                    tgccgccCAGCAGCAGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGggtggt                  >  1:5775543/2‑150 (MQ=255)

                                                                                                                                    cgccgcCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGggtggt                  <  2:4812336/150‑1 (MQ=255)

                                                                                                                                      ccgccCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCa                >  2:1015624/1‑150 (MQ=255)

                                                                                                                                       cgccCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCaa               >  2:1592269/1‑150 (MQ=255)

                                                                                                                                         cccTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATc             <  1:1380724/150‑1 (MQ=255)

                                                                                                                                             gctgctgGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATCTGAc         >  1:771513/1‑150 (MQ=255)

                                                                                                                                              ctgctgGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATCTGAcc        <  1:7247283/150‑1 (MQ=255)

                                                                                                                                                    ggcggcAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATCTGACCGGCAGt  >  1:4813729/1‑150 (MQ=255)

GTGGTATCGCGGGCGCTGCTACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATCTGACCGGCAGT  >  NC_002947/143448‑143745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`