BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	143,599	G→A	5.3%	G17S (GGC→AGC)	PP_0136 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	143,599	0	G	A	5.3%	980.3 / 28.0	301	G17S (GGC→AGC)	PP_0136	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (159/126); new base A (7/9); total (166/135)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

Only 100 of 308 total aligned reads displayed.

GTATCGCGGGCGCTGCTACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATCTGACCGGCAGTTCT  >  NC_002947/143451‑143748

gtATCGCGGGCGCTGCTACAGGTATCGACAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTCTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGggcgg                                                                                                                                                      <  2:5419706/150‑1 (MQ=255)

          cgcTGCTACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCgcggcg                                                                                                                                            >  1:2273519/1‑150 (MQ=255)

           gctgctACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCATGGCCGCCGCGCTGCCGCCCAGCAGCAGGgcggcga                                                                                                                                           <  2:4964177/150‑2 (MQ=38)

                tACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATg                                                                                                                                      >  2:7329238/1‑150 (MQ=255)

                tACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATg                                                                                                                                      >  1:6111278/1‑150 (MQ=255)

                   aGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGCCGCCGCGCTGCCGCCCAGCAGCAGGGCGGCGACCACggct                                                                                                                                   <  2:5209788/150‑2 (MQ=37)

                   aGGTATCGCCAAGATCCTGTTCAGTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATggcg                                                                                                                                   <  1:6272520/150‑1 (MQ=255)

                      gaTCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGcc                                                                                                                                <  2:3211084/149‑1 (MQ=255)

                          gCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATg                                                                                                                            >  2:1946666/1‑150 (MQ=255)

                           ccAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGa                                                                                                                           >  1:5528473/1‑150 (MQ=255)

                           ccAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGa                                                                                                                           >  2:226876/1‑150 (MQ=255)

                            cAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGAc                                                                                                                          >  1:3157363/1‑150 (MQ=255)

                             aaGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACg                                                                                                                         <  1:7390813/150‑1 (MQ=255)

                              aGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACgg                                                                                                                        >  1:1641980/1‑150 (MQ=255)

                               gATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGc                                                                                                                       >  2:6912634/1‑150 (MQ=255)

                               gATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGc                                                                                                                       >  2:1754826/1‑150 (MQ=255)

                               gATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGc                                                                                                                       >  1:3737679/1‑150 (MQ=255)

                                aTCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGcc                                                                                                                      >  1:4394524/1‑150 (MQ=255)

                                   cTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAgg                                                                                                                   >  2:6297370/1‑150 (MQ=255)

                                   cTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAgg                                                                                                                   <  1:6237161/150‑1 (MQ=255)

                                    tGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGt                                                                                                                  >  2:7247283/1‑150 (MQ=255)

                                     gTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGtt                                                                                                                 >  2:6488706/1‑150 (MQ=255)

                                     gTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGtt                                                                                                                 >  1:4191915/1‑150 (MQ=255)

                                     gTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGtt                                                                                                                 >  2:3073489/1‑150 (MQ=255)

                                      ttCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTc                                                                                                                >  2:4867147/1‑150 (MQ=255)

                                      ttCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTc                                                                                                                >  1:5775237/1‑150 (MQ=255)

                                        cATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCgg                                                                                                              <  1:3331724/150‑1 (MQ=255)

                                         aTTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCggg                                                                                                             >  2:5133366/1‑150 (MQ=255)

                                         aTTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCggg                                                                                                             >  2:586940/1‑150 (MQ=255)

                                         aTTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGAAGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCggg                                                                                                             <  2:1142020/150‑1 (MQ=255)

                                          ttGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGATGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGt                                                                                                            <  1:1656797/150‑1 (MQ=255)

                                                tCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCa                                                                                                      >  2:2805895/1‑150 (MQ=255)

                                                tCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCa                                                                                                      >  2:3511865/1‑150 (MQ=255)

                                                 ccTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCaa                                                                                                     >  1:6530862/1‑150 (MQ=255)

                                                  cTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAAc                                                                                                    >  1:5020534/1‑150 (MQ=255)

                                                   tGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACt                                                                                                   >  1:980262/1‑150 (MQ=255)

                                                   tGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACt                                                                                                   >  1:1154632/1‑150 (MQ=255)

                                                   tGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACt                                                                                                   >  1:2965473/1‑150 (MQ=255)

                                                   tGGTGATGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACt                                                                                                   >  2:5313546/1‑150 (MQ=255)

                                                     gtgtTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCCGGTTCGGGTCGACCAACTgc                                                                                                 >  1:6885784/1‑150 (MQ=255)

                                                     gtgtTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTgc                                                                                                 >  2:4281801/1‑150 (MQ=255)

                                                        ttgtttgtAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTgctcg                                                                                              >  1:1022575/1‑150 (MQ=255)

                                                          gtttgtAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGTAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTgctcggc                                                                                            >  2:6610575/1‑150 (MQ=255)

                                                                aGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGAc                                                                                      <  1:3423646/150‑1 (MQ=255)

                                                                 gCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGAcc                                                                                     >  2:3861234/1‑150 (MQ=255)

                                                                   ctcctTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAATCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCg                                                                                   >  2:3291334/1‑150 (MQ=255)

                                                                   ctcctTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCg                                                                                   <  1:5920139/150‑1 (MQ=255)

                                                                   ctcctTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCg                                                                                   >  2:6800865/1‑150 (MQ=255)

                                                                    tcctTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCgg                                                                                  >  1:5472474/1‑150 (MQ=255)

                                                                     cctTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGa                                                                                 >  1:3529985/1‑150 (MQ=255)

                                                                     cctTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGa                                                                                 <  2:5923669/150‑1 (MQ=255)

                                                                       ttcttcttTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGt                                                                               >  2:4760355/1‑150 (MQ=255)

                                                                       ttcttcttTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGt                                                                               >  1:4797547/1‑150 (MQ=255)

                                                                        tcttcttTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTa                                                                              >  1:3001284/1‑150 (MQ=255)

                                                                        tcttcttTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTa                                                                              >  1:5989075/1‑150 (MQ=255)

                                                                            cttTGGACGCGGCCGAGGGTGAAGATGAGCCGGAAGCTTCGGTCAGCCGTGGTCGCCGCCCTGCTGCGGGGCGGCAGCGCGGCGGCCATGGCGGCCACTGCCGGCCAGGTTCGGGTCGCCCAACGGCTCGGCTCGGACCCGGAGTACCgg                                                                          <  2:1476197/150‑1 (MQ=255)

                                                                            cttTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCgg                                                                          <  1:7124257/150‑1 (MQ=255)

                                                                                  aCGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAAcc                                                                    >  2:1069134/1‑150 (MQ=255)

                                                                                    gcgGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTg                                                                  <  1:840924/150‑1 (MQ=255)

                                                                                    gcgGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTg                                                                  >  2:6981074/1‑150 (MQ=255)

                                                                                     cgGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTgg                                                                 <  1:6503303/150‑1 (MQ=255)

                                                                                     cgGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTgg                                                                 <  2:828600/150‑1 (MQ=255)

                                                                                       gACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCa                                                               <  1:4867147/150‑1 (MQ=255)

                                                                                         cGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAgg                                                             <  2:6064533/150‑1 (MQ=255)

                                                                                            ggTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGaca                                                          >  2:5014415/1‑150 (MQ=255)

                                                                                             gTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGacac                                                         >  1:148241/1‑150 (MQ=255)

                                                                                              ttGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACAcc                                                        <  1:2716742/150‑1 (MQ=255)

                                                                                              ttGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACAcc                                                        >  2:1739771/1‑150 (MQ=255)

                                                                                              ttGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACAcc                                                        >  1:2002030/1‑150 (MQ=255)

                                                                                                gAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCt                                                      >  1:5796840/1‑150 (MQ=255)

                                                                                                gAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCt                                                      >  2:3387714/1‑150 (MQ=255)

                                                                                                  aGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCg                                                    >  2:5351418/1‑150 (MQ=255)

                                                                                                   gATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGa                                                   >  1:98756/1‑150 (MQ=255)

                                                                                                      gAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAgg                                                <  2:2397725/150‑1 (MQ=255)

                                                                                                      gAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAgg                                                <  1:491463/150‑1 (MQ=255)

                                                                                                        gCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCg                                              >  1:4087366/1‑150 (MQ=255)

                                                                                                         cAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGa                                             >  1:6796879/1‑150 (MQ=255)

                                                                                                         cAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGa                                             <  1:2509319/150‑1 (MQ=255)

                                                                                                          aGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGTGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAg                                            >  1:2076702/1‑150 (MQ=255)

                                                                                                            gAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGa                                          >  1:388220/1‑150 (MQ=255)

                                                                                                            gAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGa                                          >  2:2699333/1‑150 (MQ=255)

                                                                                                            gAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGa                                          >  2:562865/1‑150 (MQ=255)

                                                                                                              aGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCATGACACCCTCGAAGGCGAGGAAc                                        <  1:7032349/150‑1 (MQ=255)

                                                                                                                    gTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGc                                  <  2:1257664/150‑1 (MQ=255)

                                                                                                                        cgCCATGGCCGCCGCGCTGCCGCCCAGCAGCAGGGCGGCGACCACGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGa                              >  1:1974513/2‑150 (MQ=25)

                                                                                                                        aGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGa                              >  1:6344727/1‑150 (MQ=255)

                                                                                                                         gCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGAt                             >  1:4726843/1‑150 (MQ=255)

                                                                                                                          ccGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGAtt                            <  2:365913/150‑1 (MQ=255)

                                                                                                                             tGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTggg                         <  1:4836632/150‑1 (MQ=255)

                                                                                                                              ggTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGggt                        <  1:1745210/150‑1 (MQ=255)

                                                                                                                               gTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGggtg                       >  2:2429484/1‑150 (MQ=255)

                                                                                                                               gTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGggtg                       <  1:1291513/150‑1 (MQ=255)

                                                                                                                                  gccgccCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGaa                                                  >  1:6650297/1‑120 (MQ=255)

                                                                                                                                    cgccCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCaa                  >  2:1592269/1‑150 (MQ=255)

                                                                                                                                      cccTGCTGCTGGGCGGCAGCGCGGCGGCCATGGTGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATc                <  2:2076702/150‑1 (MQ=255)

                                                                                                                                      cccTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATc                <  2:4515438/150‑1 (MQ=255)

                                                                                                                                      cccTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATc                >  1:725512/1‑150 (MQ=255)

                                                                                                                                      cccTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATc                <  1:1380724/150‑1 (MQ=255)

                                                                                                                                               tgGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATCTGACCGGCa       <  2:4191915/150‑1 (MQ=255)

                                                                                                                                                 ggcggcAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATCTGACCGGCAGt     >  1:4579096/1‑150 (MQ=255)

GTATCGCGGGCGCTGCTACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATCTGACCGGCAGTTCT  >  NC_002947/143451‑143748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`