BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	143,604	C→G	5.2%	S18R (AGC→AGG)	PP_0136 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	143,604	0	C	G	5.2%	1055.5 / 34.3	306	S18R (AGC→AGG)	PP_0136	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (161/129); new base G (8/8); total (169/137)
Fisher's exact test for biased strand distribution p-value = 7.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 310 total aligned reads displayed.

GCGGGCGCTGCTACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATCTGACCGGCAGTTCTCAGCA  >  NC_002947/143456‑143753

     cgcTGCTACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCgcggcg                                                                                                                                                 >  1:2273519/1‑150 (MQ=255)

      gctgctACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGcggcgg                                                                                                                                                >  1:6213663/1‑150 (MQ=255)

          ctACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCAt                                                                                                                                            >  1:5213283/1‑150 (MQ=255)

            aCAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATgg                                                                                                                                          >  1:5495285/1‑150 (MQ=255)

              aGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGCCGCCGCGCTGCCGCCCAGCAGCAGGGCGGCGACCACggct                                                                                                                                        <  2:5209819/150‑2 (MQ=37)

              aGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCATGGCCGCCGCGCTGCCGCCCAGCAGCAGGGCGGCGACCACggct                                                                                                                                        <  1:3991997/150‑2 (MQ=25)

                gTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATggcggc                                                                                                                                      >  2:5395271/1‑150 (MQ=255)

                   tCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGCCGCCGCGCTGCCGCCCAGCAGCAGGgcggcgac                                                                                                                                               <  2:4904865/138‑3 (MQ=38)

                   tCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGCCGCCGCGCTGCCGCCCAGCAGCAGGgcggcgac                                                                                                                                               >  1:4904865/1‑136 (MQ=38)

                     gCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATg                                                                                                                                 >  2:1983226/1‑150 (MQ=255)

                      ccAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAAt                                                                                                                                  >  2:381179/1‑148 (MQ=255)

                      ccAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAAt                                                                                                                                  <  1:381179/148‑1 (MQ=255)

                      ccAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGa                                                                                                                                >  2:226876/1‑150 (MQ=255)

                        aaGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACg                                                                                                                              <  1:7390813/150‑1 (MQ=255)

                         aGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACgg                                                                                                                             >  1:1641980/1‑150 (MQ=255)

                          gATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGc                                                                                                                            >  2:6912634/1‑150 (MQ=255)

                              cTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAgg                                                                                                                        >  2:6297370/1‑150 (MQ=255)

                               tGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGt                                                                                                                       >  2:7247283/1‑150 (MQ=255)

                                gTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGtt                                                                                                                      >  2:6488706/1‑150 (MQ=255)

                                 ttCTTTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTc                                                                                                                     <  2:5061576/150‑1 (MQ=255)

                                   cATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCgg                                                                                                                   <  2:3537572/150‑1 (MQ=255)

                                   cATTGTCGTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCgg                                                                                                                   <  1:3287061/150‑1 (MQ=255)

                                    cttGTCGTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGCCGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCggg                                                                                                                  <  2:3886569/149‑1 (MQ=255)

                                    aTTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGAAGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCggg                                                                                                                  <  2:1142020/150‑1 (MQ=255)

                                     ttGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGATGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGt                                                                                                                 <  1:1656797/150‑1 (MQ=255)

                                      tGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTc                                                                                                                >  1:6088178/1‑150 (MQ=255)

                                      tGTCTTCCGGGGGTTGTTTGGAGCCTCCTTCTTCGTTGGACGCGGACGCGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCGGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAgggtcgggtc                                                                                                                <  2:5638893/150‑1 (MQ=255)

                                          ttCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGAcc                                                                                                            >  1:4191478/1‑150 (MQ=255)

                                           tCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCa                                                                                                           >  2:2805895/1‑150 (MQ=255)

                                              tGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACt                                                                                                        >  1:980262/1‑150 (MQ=255)

                                              tGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACt                                                                                                        >  1:1154632/1‑150 (MQ=255)

                                                gtgtTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTgc                                                                                                      >  2:4281801/1‑150 (MQ=255)

                                                gtgtTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTgc                                                                                                      >  1:6885725/1‑150 (MQ=255)

                                                gtgtTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTgc                                                                                                      <  1:6508103/150‑1 (MQ=255)

                                                 tgttgtTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTgct                                                                                                     >  2:3356965/1‑150 (MQ=255)

                                                   ttgtttgtAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTgctcg                                                                                                   >  1:1022575/1‑150 (MQ=255)

                                                   ttgtttgtAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTgctcg                                                                                                   >  2:3285810/1‑150 (MQ=255)

                                                     gtttgtAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGTAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTgctcggc                                                                                                 >  2:6610575/1‑150 (MQ=255)

                                                       ttgtAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTgctcggctc                                                                                               <  1:4994449/150‑1 (MQ=255)

                                                              ctcctTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCg                                                                                        >  2:6800865/1‑150 (MQ=255)

                                                              ctcctTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCg                                                                                        <  1:586940/150‑1 (MQ=255)

                                                              ctcctTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCg                                                                                        >  1:6616905/1‑150 (MQ=255)

                                                                  ttcttcttTGGACGCGGACGAGGTTGAAGATGAGCAGGAGGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGt                                                                                    >  1:7122579/1‑150 (MQ=255)

                                                                  ttcttcttTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGt                                                                                    >  2:4760355/1‑150 (MQ=255)

                                                                   tcttcttTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTa                                                                                   >  1:6746233/1‑150 (MQ=255)

                                                                     ttcttTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTAcc                                                                                 <  2:4997442/150‑1 (MQ=255)

                                                                     ttcttTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTAcc                                                                                 >  1:4656335/1‑150 (MQ=255)

                                                                      tcttTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCg                                                                                <  1:3424419/150‑1 (MQ=255)

                                                                       cttTGGACGCGGCCGAGGGTGAAGATGAGCCGGAAGCTTCGGTCAGCCGTGGTCGCCGCCCTGCTGCGGGGCGGCAGCGCGGCGGCCATGGCGGCCACTGCCGGCCAGGTTCGGGTCGCCCAACGGCTCGGCTCGGACCCGGAGTACCgg                                                                               <  2:1476197/150‑1 (MQ=255)

                                                                       cttTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCgg                                                                               <  1:3013502/150‑1 (MQ=255)

                                                                       cttTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCgg                                                                               <  2:587352/150‑1 (MQ=255)

                                                                            gACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAAc                                                                          <  1:6451114/150‑1 (MQ=255)

                                                                            gACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAAc                                                                          >  2:1745005/1‑150 (MQ=255)

                                                                             aCGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAAcc                                                                         >  1:700354/1‑150 (MQ=255)

                                                                             aCGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAAcc                                                                         >  2:1069134/1‑150 (MQ=255)

                                                                             aCGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAAcc                                                                         >  2:5150950/1‑150 (MQ=255)

                                                                              cgcgGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCt                                                                        <  1:3720028/150‑1 (MQ=255)

                                                                               gcgGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTg                                                                       <  1:840924/150‑1 (MQ=255)

                                                                               gcgGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTg                                                                       >  2:6981074/1‑150 (MQ=255)

                                                                                 ggACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGc                                                                     >  2:5486293/1‑150 (MQ=255)

                                                                                  gACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCa                                                                    <  1:3355320/150‑1 (MQ=255)

                                                                                   aCGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAg                                                                   >  1:4467547/1‑150 (MQ=255)

                                                                                    cGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAgg                                                                  <  2:6064533/150‑1 (MQ=255)

                                                                                     gAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGa                                                                 >  1:2979355/1‑150 (MQ=255)

                                                                                        gTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGacac                                                              >  2:1874122/1‑150 (MQ=255)

                                                                                        gTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGacac                                                              >  1:148241/1‑150 (MQ=255)

                                                                                         ttGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGaaa                                                                           <  2:3120967/136‑1 (MQ=255)

                                                                                         ttGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACAcc                                                             >  1:2002030/1‑150 (MQ=255)

                                                                                          tGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTC‑GACCCGGAGTACCGGGAAACCTGGCAGGACAccca                                                           >  1:5987247/1‑149 (MQ=255)

                                                                                             aGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCACCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCg                                                         >  2:2134009/1‑150 (MQ=255)

                                                                                              gATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGa                                                        >  1:98756/1‑150 (MQ=255)

                                                                                              gATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGa                                                        >  1:5605851/1‑150 (MQ=255)

                                                                                                 gAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAgg                                                     <  2:2397725/150‑1 (MQ=255)

                                                                                                    cAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGa                                                  <  1:2509319/150‑1 (MQ=255)

                                                                                                    cAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGa                                                  <  2:7394331/150‑1 (MQ=255)

                                                                                                    cAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGa                                                  <  1:4733802/150‑1 (MQ=255)

                                                                                                     aGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAg                                                 <  2:85027/150‑1 (MQ=255)

                                                                                                       gAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGa                                               >  2:2699333/1‑150 (MQ=255)

                                                                                                           cTTCGGTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGc                                           >  2:5336961/1‑150 (MQ=255)

                                                                                                               gTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGc                                       <  2:1257664/150‑1 (MQ=255)

                                                                                                                   cgCCATGGCCGCCGCGCTGCCGCCCAGCAGCAGGGCGGCGACCACGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGa                                   >  1:1974513/2‑150 (MQ=25)

                                                                                                                     ccGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGAtt                                 <  1:2699634/150‑1 (MQ=255)

                                                                                                                       gTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTgg                               >  2:3469516/1‑150 (MQ=255)

                                                                                                                        tGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTggg                              <  1:4836748/150‑1 (MQ=255)

                                                                                                                         ggTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGggt                             <  1:1745210/150‑1 (MQ=255)

                                                                                                                         ggTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGggt                             <  1:6254999/150‑1 (MQ=255)

                                                                                                                          gTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGggtg                            <  1:1291513/150‑1 (MQ=255)

                                                                                                                          gTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGggtg                            >  2:2429484/1‑150 (MQ=255)

                                                                                                                            tgccgccCAGCAGCAGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGggtggt                          >  1:5775543/2‑150 (MQ=255)

                                                                                                                               cgccCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCaa                       >  2:1592269/1‑150 (MQ=255)

                                                                                                                                 cccTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATc                     <  2:4515438/150‑1 (MQ=255)

                                                                                                                                 cccTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATc                     <  1:1380724/150‑1 (MQ=255)

                                                                                                                                  ccTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATCt                    <  1:431588/150‑1 (MQ=255)

                                                                                                                                      ctgctgGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATCTGAcc                <  1:7247283/150‑1 (MQ=255)

                                                                                                                                          tgGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATCTGACCGGCa            <  2:4191915/150‑1 (MQ=255)

                                                                                                                                            ggcggcAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATCTGACCGGCAGt          >  1:4813729/1‑150 (MQ=255)

                                                                                                                                             gcggcAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATCTGACCGGCAGtt         <  1:63028/150‑1 (MQ=255)

                                                                                                                                                 cAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATCTGACCGGCAGTTCTca     <  1:7329216/150‑1 (MQ=255)

                                                                                                                                                  aGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATCTGACCGGCAGTTCTcag    <  1:2740766/150‑1 (MQ=255)

                                                                                                                                                    cgcgGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATCTGACCGGCAGTTCTcagca  <  1:2134009/150‑1 (MQ=255)

GCGGGCGCTGCTACAGGTATCGCCAAGATCCTGTTCATTGTCTTCCTGGTGTTGTTTGTAGCCTCCTTCTTCTTTGGACGCGGACGAGGTTGAAGATGAGCAGGAAGCTTCGTTCAGCCGTGGTCGCCGCCCTGCTGCTGGGCGGCAGCGCGGCGGCCATGGCGGCCAATGACGGCCAGGTTCGGGTCGACCAACTGCTCGGCTCGGACCCGGAGTACCGGGAAACCTGGCAGGACACCCTCGAAGGCGAGGAACGCCTGCCCGATTGGGTGGTCAATCTGACCGGCAGTTCTCAGCA  >  NC_002947/143456‑143753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`