BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,638,547	C→G	5.3%	P169A (CCC→GCC)	lepB →	signal peptidase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,638,547	0	C	G	5.3%	1020.2 / 31.5	297	P169A (CCC→GCC)	lepB	signal peptidase I
Reads supporting (aligned to +/- strand): ref base C (161/120); new base G (3/13); total (164/133)
Fisher's exact test for biased strand distribution p-value = 3.42e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 311 total aligned reads displayed.

TCATCGAGGTCGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGACCGTGCCTGCG  >  NC_002947/1638398‑1638696

tCATCGAGGTCGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGc                                                                                                                                                       >  2:5286617/1‑150 (MQ=255)

  aTCGAGGTCGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGccc                                                                                                                                                     >  1:5868090/1‑150 (MQ=255)

  aTCGAGGTCGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGccc                                                                                                                                                     >  2:6050935/1‑150 (MQ=255)

  aTCGAGGTCGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGccc                                                                                                                                                     >  1:1617756/1‑150 (MQ=255)

  aTCGAGGTCGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGccc                                                                                                                                                     >  2:3765150/1‑150 (MQ=255)

   tCGAGGTCGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGcccgcccg                                                                                                                                                    >  1:5058932/1‑150 (MQ=255)

        gTCGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGcc                                                                                                                                               <  1:7267014/150‑1 (MQ=255)

        gTCGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGcc                                                                                                                                               >  1:1448977/1‑150 (MQ=255)

        gTCGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGGCAACGGGCTGGCCGTTgac                                                                                                                                               <  2:3199777/150‑3 (MQ=255)

        gTCGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGACAACGGGCTGGCCGTTgac                                                                                                                                               <  2:3608804/150‑3 (MQ=255)

         tCGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCg                                                                                                                                              <  2:2563400/150‑1 (MQ=255)

          cGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGGCAACGGGCTGGCCGTTGACGa                                                                                                                                             <  1:1701568/150‑1 (MQ=255)

           ggTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGGCAACGGGCTGGCCGTTGACGaa                                                                                                                                            <  1:4617030/150‑1 (MQ=255)

                cccGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGGCAACGGGCTGGCCGTTGACGaacagcc                                                                                                                                       <  2:1863001/150‑4 (MQ=255)

                 ccGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACtg                                                                                                                                      >  1:6747850/1‑150 (MQ=255)

                 ccGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCAGAATGTCAACGACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTGCGGCAACGGCCACCCCCTAGCCGcacacaag                                                                                                                                      >  1:6375050/1‑146 (MQ=255)

                   gCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACtggt                                                                                                                                    <  1:2329158/150‑1 (MQ=255)

                     aaCGCGGGGATGTAATGGTGTTCCGCGCCCCGAGTGACCCGCATGTCAACTCCCGCCAGCGAGGGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCGAGCCCGTTTCCGAACTACtggtgg                                                                                                                                  <  2:5956267/150‑1 (MQ=255)

                      aCGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTggg                                                                                                                                 <  2:3636701/150‑1 (MQ=255)

                                gTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCg                                                                                                                       >  2:4428018/1‑150 (MQ=255)

                                gTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCg                                                                                                                       <  2:6357646/150‑1 (MQ=255)

                                 tAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCgg                                                                                                                      <  1:1413527/150‑1 (MQ=255)

                                  aaTGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCCGCGCGCCggg                                                                                                                     >  1:1029200/1‑150 (MQ=255)

                                  aaTGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCATGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCggg                                                                                                                     >  1:524718/1‑150 (MQ=255)

                                   aTGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACTGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGt                                                                                                                    <  2:3250835/150‑1 (MQ=255)

                                         ttCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTg                                                                                                              >  1:6794997/1‑150 (MQ=255)

                                         ttCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTg                                                                                                              <  1:5891157/150‑1 (MQ=255)

                                           ccGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTggg                                                                                                            <  1:4495901/150‑1 (MQ=255)

                                           ccGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTggg                                                                                                            <  2:7056856/150‑1 (MQ=255)

                                           ccGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTggg                                                                                                            <  2:284932/150‑1 (MQ=255)

                                           ccGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTggg                                                                                                            >  1:436165/1‑150 (MQ=255)

                                           ccGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTggg                                                                                                            <  2:4082630/150‑1 (MQ=255)

                                             gCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCa                                                                                                          <  1:108256/150‑1 (MQ=255)

                                             gCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCa                                                                                                          >  1:2147837/1‑150 (MQ=255)

                                                  ccGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCCGCCCGTTGCCGAACAACAGGTGGGCAGCGAGCCGGGTACCCAGGGCAGTGCt                                                                                                     >  1:7204493/1‑150 (MQ=255)

                                                   cGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTg                                                                                                              >  2:5724115/1‑140 (MQ=255)

                                                    gAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGACCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCa                                                                                                   >  1:4912305/1‑150 (MQ=255)

                                                     aGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAg                                                                                                  <  1:5517117/150‑1 (MQ=255)

                                                       tGACCCGACTGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCt                                                                                                <  2:483893/150‑1 (MQ=255)

                                                          cccGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTa                                                                                             <  2:599162/150‑1 (MQ=255)

                                                          cccGAATGTCAACGACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTa                                                                                             <  2:5027195/150‑1 (MQ=255)

                                                           ccGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTAc                                                                                            >  1:889241/1‑150 (MQ=255)

                                                             gAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACaa                                                                                          <  2:4223858/150‑1 (MQ=255)

                                                                 gTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGaa                                                                                      <  1:2400734/150‑1 (MQ=255)

                                                                    aaCTACATCACGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAg                                                                                   <  2:5508916/150‑1 (MQ=255)

                                                                    aaCTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTAcc                                                                                                                 >  2:6223580/1‑120 (MQ=255)

                                                                    aaCTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTAcc                                                                                                                 <  1:6223507/120‑1 (MQ=255)

                                                                    aaCTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAg                                                                                   <  1:1339232/150‑1 (MQ=255)

                                                                         cATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCgg                                                                                                                      >  1:6041810/1‑110 (MQ=255)

                                                                          aTCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCCGCGCGCCGGGTACCCGGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGgc                                                                             >  1:654549/1‑150 (MQ=255)

                                                                          aTCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAACCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGgc                                                                             >  1:1088509/1‑150 (MQ=255)

                                                                             aaGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGcgcg                                                                          >  2:1548513/1‑150 (MQ=255)

                                                                              aGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCgg                                                                         <  1:7052096/150‑1 (MQ=255)

                                                                               gCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGCACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGc                                                                        >  2:6953594/1‑150 (MQ=255)

                                                                               gCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGc                                                                        >  1:1916566/1‑150 (MQ=255)

                                                                                 gtgtGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCCGCCCGTTGCCGAACAACTGGGGGGCAGCGAGCCGGGTACCCGGGGCAGTGCTCAGCTGTACAAGGAAAAGCTAGGCGCGGCCg                                                                      >  1:7020962/1‑150 (MQ=255)

                                                                                 gtgtGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCg                                                                      >  1:6683073/1‑150 (MQ=255)

                                                                                   gtgGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTg                                                                                                              <  1:2697778/108‑1 (MQ=255)

                                                                                   gtgGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTg                                                                                                              >  2:2697778/1‑108 (MQ=255)

                                                                                     ggTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAaca                                                                  >  2:5415583/1‑150 (MQ=255)

                                                                                      gTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAacac                                                                 >  2:6418108/1‑150 (MQ=255)

                                                                                      gTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAacac                                                                 >  2:7429901/1‑150 (MQ=255)

                                                                                          gCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGa                                                             >  2:1086955/1‑150 (MQ=255)

                                                                                          gCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGa                                                             <  1:1572878/150‑1 (MQ=255)

                                                                                            cTGCCGGGCGACGTGGTCCGCTACACCAACGACTAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATc                                                           >  2:3411137/1‑150 (MQ=255)

                                                                                            cTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATc                                                           <  1:350132/150‑1 (MQ=255)

                                                                                               ccGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGc                                                        >  2:1357078/1‑150 (MQ=255)

                                                                                               ccGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGc                                                        >  1:5463540/1‑150 (MQ=255)

                                                                                                 gggCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCCACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGACCACCTGCTCCGCac                                                      >  2:2493478/1‑149 (MQ=255)

                                                                                                 gggCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCaa                                                      <  2:2213519/150‑1 (MQ=255)

                                                                                                  ggCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAg                                                     >  1:2934431/1‑150 (MQ=255)

                                                                                                         tGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGa                                              <  1:3359238/150‑1 (MQ=255)

                                                                                                         tGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGa                                              <  1:3301123/150‑1 (MQ=255)

                                                                                                         tGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGa                                              <  1:2968086/150‑1 (MQ=255)

                                                                                                            tCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCCGCGAGCCGGGTACCCTGGGCAGTGCTCCGCTGTACCAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGcc                                           >  2:1236217/1‑150 (MQ=255)

                                                                                                            tCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGcc                                           <  1:3606204/150‑1 (MQ=255)

                                                                                                            tCCGCTACACCAACGACAAGCGGATGTTCGTCAACGGCCAGCCCGTTGCCGAAAAACTGGTGGGCAGCGACCCGGGAACCCTGGGCAGTGCACAGCTGTACAAGGAAAACCACGGCGCGGCCGAACACCTCATCCGCAAGGAAATGAccc                                           >  2:5114078/1‑150 (MQ=255)

                                                                                                                   cacCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCg                                    >  2:3915565/1‑150 (MQ=255)

                                                                                                                      cAACGACAAGCGGCTGTTCGTCAACGGCCCGCCCGTTGCCGAACAACAGGTGGGCAGCGAGCCGGGTACCCAGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCCCAAGGAAATGAGCCCTTATCGCAt                                 >  1:382773/1‑150 (MQ=255)

                                                                                                                      cAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCAt                                 >  1:3071309/1‑150 (MQ=255)

                                                                                                                      cAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCGCAGCGGTACAAGGAAAAGCTCGGCGCGGCCGCACACCTGATCCGCAAGGAAATGAGCCGTTATCGCag                                 >  1:5182986/1‑149 (MQ=255)

                                                                                                                       aaCGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATg                                >  2:336626/1‑150 (MQ=255)

                                                                                                                          gACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGACATGAGCCGTTATCGCATgccg                             >  2:6776616/1‑150 (MQ=255)

                                                                                                                           aCAAGCGGCTGTTCGTCAACGGCCAGCCCCTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCAGATCCGCAAGGAAATGAGCCGTTATCGCATgccgc                            >  1:754135/1‑150 (MQ=255)

                                                                                                                             aaGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGccgccg                          <  1:4448315/150‑1 (MQ=255)

                                                                                                                               gCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAAATGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGa                        >  2:4706100/1‑150 (MQ=255)

                                                                                                                                 ggCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGAcc                      >  2:1024485/1‑150 (MQ=255)

                                                                                                                                    tGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACcagc                   >  2:72049/1‑150 (MQ=255)

                                                                                                                                      ttCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACcagcag                 >  1:6917979/1‑150 (MQ=255)

                                                                                                                                       tCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGt                <  2:160776/150‑1 (MQ=255)

                                                                                                                                       tCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGt                <  1:4074403/150‑1 (MQ=255)

                                                                                                                                       tCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGt                <  1:5763972/150‑1 (MQ=255)

                                                                                                                                         gTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTgg              >  2:6657411/1‑150 (MQ=255)

                                                                                                                                            aaCGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGAcc           >  1:4284750/1‑150 (MQ=255)

                                                                                                                                            aaCGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGAcc           >  2:4630175/1‑150 (MQ=255)

                                                                                                                                            aaCGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGAcc           >  2:872754/1‑150 (MQ=255)

                                                                                                                                            aaCGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCACCGGACCAGCAGTGGAcc           >  2:3286306/1‑150 (MQ=255)

                                                                                                                                                  cAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGACCGTGCCt     >  2:7279768/1‑150 (MQ=255)

                                                                                                                                                  cAGCCCGTTGCCGAACAACTGGCGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGACATGAGCCGGTATCGCATGCCGCCGGACCAGCAGTGGACCGTGCCt     >  2:7016902/1‑150 (MQ=255)

                                                                                                                                                    gCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGACCGTGCCTGc   >  2:301857/1‑150 (MQ=255)

TCATCGAGGTCGGTGACCCGCAACGCGGGGATGTAATGGTGTTCCGCTACCCGAGTGACCCGAATGTCAACTACATCAAGCGTGTGGTTGGCCTGCCGGGCGACGTGGTCCGCTACACCAACGACAAGCGGCTGTTCGTCAACGGCCAGCCCGTTGCCGAACAACTGGTGGGCAGCGAGCCGGGTACCCTGGGCAGTGCTCAGCTGTACAAGGAAAAGCTCGGCGCGGCCGAACACCTGATCCGCAAGGAAATGAGCCGTTATCGCATGCCGCCGGACCAGCAGTGGACCGTGCCTGCG  >  NC_002947/1638398‑1638696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`