Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,847,803 T→G 6.8% N661T (AAC→ACC)  gacS ← sensor protein GacS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,847,8030TG 6.8% 718.5 / 4.0 246N661T (AAC→ACC) gacSsensor protein GacS
Reads supporting (aligned to +/- strand):  ref base T (90/132);  new base G (21/3);  total (111/135)
Fisher's exact test for biased strand distribution p-value = 1.34e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

TTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGCACA  >  NC_002947/1847655‑1847950
                                                                                                                                                    |                                                                                                                                                   
  cttcCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGc                                                                                                                                                  <  2:4430966/150‑1 (MQ=255)
  cttcCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGc                                                                                                                                                  >  2:1939108/1‑150 (MQ=255)
  cttcCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGc                                                                                                                                                  >  2:3171681/1‑150 (MQ=255)
   ttcCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCt                                                                                                                                                 >  1:3599467/1‑150 (MQ=255)
   ttcCTGCACGGCCTGTACCGCCGCATAACCAGTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCt                                                                                                                                                 <  2:6858918/150‑1 (MQ=255)
    tcCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTc                                                                                                                                                >  2:1178661/1‑150 (MQ=255)
    tcCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTc                                                                                                                                                <  2:4809186/150‑1 (MQ=255)
      cTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTccc                                                                                                                                              <  1:1105747/150‑1 (MQ=255)
      cTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTccc                                                                                                                                              >  2:3859983/1‑150 (MQ=255)
      cTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGGTGGGGGTGCTccc                                                                                                                                              >  1:2495802/1‑150 (MQ=255)
        gCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGGTGGGGTTGCTacccc                                                                                                                                            >  1:4089282/1‑149 (MQ=255)
          aCGGCTTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGTCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCg                                                                                                                                          <  2:3550239/150‑1 (MQ=255)
           cGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCgg                                                                                                                                         <  2:3402634/150‑1 (MQ=255)
             gCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGGCGGTGGGGTTGCTCCCCGCGGcc                                                                                                                                       >  1:5248508/1‑150 (MQ=255)
              ccTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGccc                                                                                                                                      >  2:3742388/1‑149 (MQ=255)
               cTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAg                                                                                                                                     <  2:4202831/150‑1 (MQ=255)
                tGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGc                                                                                                                                    <  1:2746504/150‑1 (MQ=255)
                    ccgccgCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGAc                                                                                                                                <  1:3846863/150‑1 (MQ=255)
                      gccgcATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCGGGCCGGTTGGGGTGCTCCCCACGGCCCGCGGACgg                                                                                                                              >  1:6404957/1‑150 (MQ=255)
                         gcATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGAGTTGCTCCCCTCGGCCAGCGGACGGCGa                                                                                                                           <  1:801367/150‑1 (MQ=255)
                         gcATAACCATTGTTGACGCCCAGTACCACGGCACACAGGTATACGAGCAGGGATTTTACCAGCAGCAGGTTGGCGCCATTGTCGTCGACTCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGa                                                                                                                           <  2:6426473/150‑1 (MQ=255)
                           aTAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCCCCGGACGGCGAgg                                                                                                                         >  2:285886/1‑150 (MQ=255)
                            tAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGt                                                                                                                        >  2:575063/1‑150 (MQ=255)
                                cATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGGTGCTCCCCGCCGCCAGCGGACGGCGAGGGTGCa                                                                                                                    >  2:10237/1‑150 (MQ=255)
                                   tgttgACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGGTGCTCCCCTCGGCCCGCGGACGGCGAGGTTGCACCa                                                                                                                 >  2:7406521/1‑150 (MQ=255)
                                     ttgACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTCCCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGc                                                                                                               <  1:5385370/150‑1 (MQ=255)
                                     ttgACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCCGCGGACGGCGAGGTTGCACCAGc                                                                                                               >  2:4103152/1‑150 (MQ=255)
                                      tgACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGGGGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGGTGCACCAGcg                                                                                                              >  2:5025079/1‑149 (MQ=255)
                                       gACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTc                                                                                                             <  1:5898611/150‑1 (MQ=255)
                                        aCGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCGGGGCGGTGGGGGTGGGCCCCGCCGCCACCGGACGGGGCGGTTGCAcccccgcc                                                                                                            >  1:6880168/1‑144 (MQ=255)
                                           gCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGc                                                                                                         <  2:6120661/150‑1 (MQ=255)
                                             cAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCaa                                                                                                       <  1:396189/150‑1 (MQ=255)
                                              aGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACAAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAag                                                                                                      <  2:818873/150‑1 (MQ=255)
                                               gTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAaga                                                                                                     <  2:4533574/150‑1 (MQ=255)
                                                    tCGGCACCCAGGTCTACGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCAGGCCCTTGGGGTTGCTCCCCTCGGCCAACGGACGGCGAGGTTGCACCAGCACCACCAAGAGgcgc                                                                                                >  2:1705852/1‑150 (MQ=255)
                                                     cGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGcgcc                                                                                               <  2:5657086/150‑1 (MQ=255)
                                                      ggCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCGGGCCGGGGGGGGTGGTACCCACGGCCACCGGGCGGCCAGGTTTGAACACCGACACCAAGAGGcgccc                                                                                              >  1:1412464/1‑149 (MQ=255)
                                                        cACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCa                                                                                            <  2:5100882/150‑1 (MQ=255)
                                                          cccAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGc                                                                                          <  2:2747935/150‑1 (MQ=255)
                                                           ccAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCt                                                                                         >  2:6350138/1‑150 (MQ=255)
                                                           ccAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGGTGGGGTTGCTCCCCGCGGCCCGCGGCCGGCGAGGTTGCACCAGCGCCCCCCAGAGGCGCCGCAGCt                                                                                         >  2:239691/1‑150 (MQ=255)
                                                                tCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCgg                                                                                    <  1:1762851/150‑1 (MQ=255)
                                                                tCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCgg                                                                                    <  1:4537038/150‑1 (MQ=255)
                                                                 cTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGGTGGGGTTGCTACCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCggg                                                                                   >  2:2277644/1‑150 (MQ=255)
                                                                  ttCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGGTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTAGCgggg                                                                                  >  2:6106694/1‑149 (MQ=255)
                                                                    cGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGc                                                                                <  1:2812183/150‑1 (MQ=255)
                                                                    cGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGc                                                                                <  2:7276028/150‑1 (MQ=255)
                                                                     gAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCGTGCGGGTGCa                                                                               >  2:7069544/1‑150 (MQ=255)
                                                                      aGCAGGGATTTTCCCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGGTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAg                                                                              <  2:5344236/150‑1 (MQ=255)
                                                                         aGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTGGCTGGCCGGTGGGGGTGCTCCCCGCCGCCCGCGGCCGGCGAGGTTGCACCAGCGACACCCCGAGGCGCCGCAGCTTTCGGGTGGGGGTc                                                                           >  1:5161940/1‑150 (MQ=255)
                                                                           ggTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCgg                                                                         <  1:211786/150‑1 (MQ=255)
                                                                           ggTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCgg                                                                         <  2:670598/150‑1 (MQ=255)
                                                                           ggTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCGGGCCGTTTGGGTTGGTCCCCGCCGCCCCCGGACGGCGCGGGTTCACCACCTCCAGCAAGAGGGCCCCCAGCTTGCGGGGGCAgggggg                                                                         >  1:4694473/1‑146 (MQ=255)
                                                                             tttttACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTGGCTGGCCGGTGGGGTTGCTCCCCCCCGGCCCCGGACGGCGCGGGTTCACCCGCACCACCAAGAGGCGCCGCAGCGTGCGGGTGCAGggcggcg                                                                       >  1:5133904/1‑149 (MQ=255)
                                                                             tttttACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCt                                                                       <  2:2342689/150‑1 (MQ=255)
                                                                              ttttACCAGCAGCTGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCGCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCtt                                                                      <  1:5320855/150‑1 (MQ=255)
                                                                              ttttACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCtt                                                                      <  2:4889563/150‑1 (MQ=255)
                                                                                   ccAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTGCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACa                                                                 <  1:3133296/150‑1 (MQ=255)
                                                                                    cagcagcagGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAg                                                                <  1:6315859/150‑1 (MQ=255)
                                                                                    cagcagcagGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGGTGGGGTTGCTCCCCTCGGCCAGCGGCCGGCGGGGTTGCACCAGCTCCCGCAAGAGGCGCCGCCGCTTGCGGGTGCAGGTCGGCTTGGACAg                                                                >  2:2216401/1‑150 (MQ=255)
                                                                                    cagcagcagGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCGGGCCGGTGGGGTTGCTACCCCCGGCCCCCGGACCGCGCCGGTGCACCACCGCCACCCACAGGCGCCCCCCCCTGCGGGTGCCGGTCGGCGTgggccc                                                                >  1:4451475/1‑146 (MQ=255)
                                                                                       cagcagGTGGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCaa                                                             <  1:2660261/150‑1 (MQ=255)
                                                                                        agcagGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCCCTGGCCGGTTGGGTTGCTCCCCACGGCCCCCGGACGGCGAGGTTGCACCAGCTCCCGCAAGAGGCGCCCCAGCGTGCGGGTGCAGGTCGGCTTGGACACCAAc                                                            >  2:4374577/1‑150 (MQ=255)
                                                                                          cagGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTTGCCGTTTGGGTTTCTCCCCTCGGCCAGCGGACGGCGGGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAAcag                                                          >  2:1397360/1‑148 (MQ=255)
                                                                                          cagGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGGGGTTTGGGTTTGTACCCTCGGCCAGCGGAAGGGGAGGTTTGAGCAGCTACATCAATAGGGGCCGCAGCTTTCGGGTGGATGTGGGGTTGGTAAGCaaatt                                                          >  1:6081738/1‑147 (MQ=255)
                                                                                              ttGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCa                                                      <  1:877716/150‑1 (MQ=255)
                                                                                                 gcggcATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATgg                                                   <  2:129877/150‑1 (MQ=255)
                                                                                                 gcggcATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATgg                                                   >  2:4952443/1‑150 (MQ=255)
                                                                                                   ggcATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGcc                                                 <  2:1524441/150‑1 (MQ=255)
                                                                                                     cATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGGTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACAGGCCATGGCCGt                                               >  2:5175830/1‑150 (MQ=255)
                                                                                                      aTTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGtt                                              <  2:5924748/150‑1 (MQ=255)
                                                                                                       ttGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTg                                             <  1:3559010/150‑1 (MQ=255)
                                                                                                       ttGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTg                                             <  2:5855061/150‑1 (MQ=255)
                                                                                                         gtcgtcGACGCAGAGTATTTTCGGGCGTCGCTGGCCGTTGGGGGTGCTCCCCTCGGCCAGCGGCCGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTggg                                           <  2:321770/150‑1 (MQ=255)
                                                                                                            gtcgACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGgcg                                                                                                 >  2:5539195/1‑93 (MQ=255)
                                                                                                            gtcgACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAg                                        <  1:4419543/150‑1 (MQ=255)
                                                                                                            gtcgACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAg                                        <  1:5872997/150‑1 (MQ=255)
                                                                                                            gtcgACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTACTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAg                                        <  1:2121449/150‑1 (MQ=255)
                                                                                                                  ctagtttATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACgg                                  <  1:251192/144‑1 (MQ=255)
                                                                                                                     gagTATTTTCGGTCGTCGCTGGCCGTTTGGGTTTCTACCCGCGGCCAGCGGACGGCGCGGTTGCAACAACAACAGCAAGATGCGCCGCAGCATTCGGGTGCAGGTCGGCGTGGTCAGCCACAGGCCAAGGCCGTTGGGGAGGTaacggag                               >  2:251192/1‑144 (MQ=255)
                                                                                                                     gagTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGggtg                               <  1:3055520/150‑1 (MQ=255)
                                                                                                                      agTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGggtgg                              <  1:1178661/150‑1 (MQ=255)
                                                                                                                       gTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGggtggt                             <  2:1847093/150‑1 (MQ=255)
                                                                                                                        tATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTa                            <  2:5413456/150‑1 (MQ=255)
                                                                                                                            ttCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAgc                        <  2:177912/150‑1 (MQ=255)
                                                                                                                               ggTCGTCGCTTGCCCGTGGGGTTTCTCCCCGAGGCCAGCGGACCGCGAGGTTTCACCAACAACACCAAGATGCGCCCCAGCTTTCGGGGGCAGGTCGGCTTGGACAGCAACAGGCCAAGGCCGGTGGGCAGGTACGGGGGGTACaccccc                     >  2:4329634/1‑145 (MQ=255)
                                                                                                                                gtcgtcGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCt                    <  1:1342328/150‑1 (MQ=255)
                                                                                                                                   gtcgCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCt                 <  1:5719070/150‑1 (MQ=255)
                                                                                                                                     cgCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCg               >  2:3794185/1‑150 (MQ=255)
                                                                                                                                      gCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCCGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCgg              >  2:466723/1‑150 (MQ=255)
                                                                                                                                       cTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCggt             >  2:5916039/1‑150 (MQ=255)
                                                                                                                                       cTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCggt             <  1:2419058/150‑1 (MQ=255)
                                                                                                                                        tGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCggtg            <  2:2487363/150‑1 (MQ=255)
                                                                                                                                        tGGCCGGTGGGGGTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCggtg            <  1:5666436/150‑1 (MQ=255)
                                                                                                                                           ccGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTc         <  1:7164313/150‑1 (MQ=255)
                                                                                                                                           ccGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTc         >  1:6248810/1‑150 (MQ=255)
                                                                                                                                            cGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGGCg        >  1:4760544/1‑150 (MQ=255)
                                                                                                                                              ttGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCggg      <  1:543340/150‑1 (MQ=255)
                                                                                                                                               tGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGc     <  2:3599467/150‑1 (MQ=255)
                                                                                                                                                ggggTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGca    <  1:3292942/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGCACA  >  NC_002947/1847655‑1847950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑