BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,276,255	C→G	5.8%	intergenic (+84/‑136)	PP_2002 → / → PP_t31	oxidoreductase/tRNA‑Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,276,255	0	C	G	5.8%	664.3 / 23.1	207	intergenic (+84/‑136)	PP_2002/PP_t31	oxidoreductase/tRNA‑Val
Reads supporting (aligned to +/- strand): ref base C (118/69); new base G (7/5); total (133/74)
Fisher's exact test for biased strand distribution p-value = 7.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

Only 100 of 219 total aligned reads displayed.

GCATTTGTCACCGGCCAGGAGTTTGTGGTCGATGGCGGCATGACCCGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAACCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAACATCGCGAGGGTGATTAGC  >  NC_002947/2276106‑2276401

 cATTTGTCACCGGCCAGGAGTTTGTGGTCGATGGCGGCATGACCCGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAACCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTAcc                                                                                                                                                    <  2:6836650/150‑1 (MQ=255)

   tttGTCACCGGCCAGGAGTTTGTGGTCGATGGCGGCATGACCCGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCGAATCGCCGCTAAACCCCCACCAACA‑AACGTTAATGTCCCACCGTAGGAACCGGTTTAaccc                                                                                                                                                  >  2:300699/1‑149 (MQ=255)

    ttGTCACCGGCCAGGAGTTTGTGGTCGATGGCGGCATGACCCGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAACCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCcgc                                                                                                                                                 <  1:2528039/150‑1 (MQ=255)

       tCACCGGCCAGGAGTTTGTGGTCGATGGCGGCATGACCCGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGTCGGACATTAACCG‑TTGTAGGAGCGGGTTTAGCCGCGaa                                                                                                                                              <  2:182929/150‑1 (MQ=18)

       tCACCGGCCAGGAGTTTGTGGTCGATGGCGGCATGACCCGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAACCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGaa                                                                                                                                              <  2:5820643/150‑1 (MQ=255)

       tCACCGGCCAGGAGTTTGTGGTCGATGGCGGCATGACCCGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAACCCGCTCCTACA‑ACGGTTAAAGTCCGACTGTAGGAGCGGGTTTACCCGCGaa                                                                                                                                              >  2:5304082/1‑150 (MQ=255)

         aCCGGCCAGGAGTTTGTGGTCGATGGCGGCATGACCCGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAACCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAaga                                                                                                                                            <  2:5637450/150‑1 (MQ=255)

         aCCGGCCAGGAGTTTGTGGTCGATGGCGGCATGACCCGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAAACCGCTCCTAAA‑AAGGTTTATGTGCGAGTGTTTGAGCGGGTTTTACCGCGAagt                                                                                                                                            >  1:4242347/1‑149 (MQ=255)

         aCCGGCCAGGAGTTTGTGGTCGATGGCGGCATGACCCGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTAGCGGCTAAAACCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGaata                                                                                                                                            >  2:2270803/1‑148 (MQ=255)

         aCCGGCCAGGAGTTTGTGGTCGATGGCGGCATGACCCGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCTATTCGCGGCTAAACCCGCTCCTAAA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAaga                                                                                                                                            >  2:2613287/1‑150 (MQ=255)

          ccGGCCAGGAGTTTGTGGTCGATGGCGGCATGACCCGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAACCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAagag                                                                                                                                           <  2:808064/150‑1 (MQ=255)

          ccGGCCAGGAGTTTGTGGTCGATGGCGGCATGACCCGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCAGCCTCTTCGCGGCTAAACCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAagag                                                                                                                                           >  2:4297601/1‑150 (MQ=255)

              ccAGGAGTTTGTGGTCGATGGCGGCATGACCCGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAACCCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCg                                                                                                                                       <  2:3767823/150‑1 (MQ=255)

              ccAGGAGTTTGTGGTCGATGGCGGCATGACCCGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCGCTTCGCGGCTAAACCCGCACCTACA‑ACGGTTAAAGTACGACGGGAGGAGCGGGTTTACCCGCCAAGAGGCCg                                                                                                                                       >  1:2316266/1‑150 (MQ=255)

               cAGGAGTTTGTGGTCGATGGCGGCATGACCCGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAAACCGCTCCTACA‑AAGGTTAATGTGCGTCTGTAGGAGCGGGTTTTACCGGGAAGAGGCCgg                                                                                                                                      >  1:1112294/1‑150 (MQ=255)

                      ttGTGGTCGATGGCGGCATGACCCGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAAACCGCTCCAACA‑ACCGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGc                                                                                                                               >  1:4914594/1‑150 (MQ=255)

                         tgGTCGATGGCGGCATGACCCGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAACCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCaaa                                                                                                                            >  2:6692597/1‑149 (MQ=255)

                           gTCGATGGCGGCATGACCCGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAACCCGCACCTACA‑ACGGTTAATGTGCGACGGGAGGAGCGGGGTTACCCCCGGAGAGGCCGGCACAGGCaaaaa                                                                                                                          >  1:1725147/1‑147 (MQ=255)

                                 ggcggaATGACCCGAAAGATGATCTATACCTGAGCACCTTTTTTAAACTGCGCCGGCCTCTTCGCGGCTAAACCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCTAAGAGGCCGGCACAGGCAatcacatcac                                                                                                                    <  2:5254140/150‑1 (MQ=255)

                                    ggcATGACCCGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAACCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGtt                                                                                                                 >  2:3638787/1‑150 (MQ=255)

                                       aTGACCCGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAACCCGCTCCTACA‑CCGTTTAATGTCCGACTGTAGGAGCGGTTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGcc                                                                                                              <  2:3414598/150‑1 (MQ=255)

                                            ccGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAACCCGCTCCTACA‑CCGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCttttt                                                                                                         <  2:3052727/150‑1 (MQ=255)

                                            ccGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAACCCGCTCCTACA‑ACGGTTAATGTCCGACTGTTGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCttttt                                                                                                         <  1:4264016/150‑1 (MQ=255)

                                            ccGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAACCCGCTCCAACA‑ACGGTTAATGTCCGACTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAAGCACATCACGATGCCttttt                                                                                                         >  2:5318900/1‑150 (MQ=255)

                                              gCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAAACCGCTCCTAAA‑AAGGTTAATTTGCGACTGTTGGTGCGGGTTTTCCCGCGAAGTTGCCGGCACAGGCAATAACATAACGTTGGCTTTTTgt                                                                                                       >  2:2204979/1‑149 (MQ=255)

                                                     gatCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAACCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAAt                                                                                               >  2:6141231/1‑150 (MQ=255)

                                                         tataCCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAACCCGCTCCTACA‑ACGGTTAATTTCCGACTGTAGGAGCGGGTTTACCCGCGGAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCaa                                                                                           >  2:4115469/1‑150 (MQ=255)

                                                         tataCCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAACCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACACGCAATCACATCACCTTGCCTTTTTG‑AAAAAAATTCaa                                                                                           >  2:3862984/1‑150 (MQ=255)

                                                          ataCCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAACCCGCTCCTACA‑ACGTTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAAt                                                                                          <  2:4406570/150‑1 (MQ=255)

                                                              cTGAGCACCTTGTTTAAACTGCGCCGGCCGCTTCGCGGCTAAAACCCCTACAAAA‑AACGTTAAAGTACGACTGTATGAGCGGGTTTAACCCCGAAGAGGCCCGCCCACGCAAAAACAAAACCTTTCAATTTTG‑AAAAAAAATAAATgggg                                                                                      >  2:1263185/1‑150 (MQ=255)

                                                                 aGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAACCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTAc                                                                                                                                                     >  2:1497345/1‑85 (MQ=255)

                                                                      cTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAACCCGCTCCTACC‑ACGGTTAATGTCCGACGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGAc                                                                              >  2:2949607/1‑150 (MQ=255)

                                                                      cTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAACCCGCTCCTACA‑ACGGTTAAAGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGCACAGGCAATCACATCACCTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGAc                                                                              >  2:7092066/1‑150 (MQ=255)

                                                                      cTTGTTTAAACTGCGCCGGCCACTTCGCGGCAAAACCCCCTACTAAA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACACGCAATAACAACACCGTGCCTTTTTG‑AAAAAAATTCAAAGGGGCGATTGAc                                                                              >  2:4055082/1‑150 (MQ=37)

                                                                           ttAAACTGCGCCGGCCTCTTCGCGGCTAAACCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGc                                                                         <  2:4914594/150‑1 (MQ=255)

                                                                              aaCTGCGCCGGCCTCTTCGCGGCTAAACCCGCTCCTACA‑ACGGTTAAGGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTc                                                                      <  2:1385653/150‑1 (MQ=255)

                                                                                     ccGGCCTCTCCGCGGCTAAACCCGCCCCCACA‑CCGTTAAATGTCCGACTTTAGGAGGGGTTTCACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCt                                                               <  1:6476144/150‑1 (MQ=255)

                                                                                           tctTCGCGGCTAAACCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTGAAAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTa                                                          >  2:5718689/1‑150 (MQ=255)

                                                                                           tctTCGCGGCTAAACCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTaa                                                         >  2:4759776/1‑150 (MQ=255)

                                                                                           tctTCGCGGCTAAACCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTaa                                                         >  2:6491198/1‑150 (MQ=255)

                                                                                            ctTCGCGGCTAAACCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGTTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTaaa                                                        <  1:5512047/150‑1 (MQ=255)

                                                                                             ttCGCGGCTAAACCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACCTAGCTTCGCCACCTGCGTAAAt                                                       >  1:1139062/1‑150 (MQ=255)

                                                                                                  ggCTAAACCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTcgc                                                  <  2:7392017/150‑1 (MQ=255)

                                                                                                     tAAACCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGc                                               >  2:835723/1‑150 (MQ=255)

                                                                                                       aaCCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCt                                             >  2:7127123/1‑150 (MQ=255)

                                                                                                       aaCCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCt                                             >  1:5754226/1‑150 (MQ=255)

                                                                                                        aCCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTc                                            >  2:1244555/1‑150 (MQ=255)

                                                                                                        aCCCGCACCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTAGCAGCGAAGAGGCCGGCACAGGCACTCACATCACGTTGCCTTTTTG‑AACAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTc                                            >  2:903243/1‑150 (MQ=255)

                                                                                                         cccGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCa                                           >  2:3174132/1‑150 (MQ=255)

                                                                                                          ccGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAg                                          >  2:7352451/1‑150 (MQ=255)

                                                                                                          ccGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAg                                          >  2:171897/1‑150 (MQ=255)

                                                                                                           cGCTCCTACA‑AGGTTTAATGTCCGACTGTTGGAGGGGGTTTACCCGCTAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGc                                         <  1:3803136/150‑1 (MQ=255)

                                                                                                            gCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCg                                        >  1:6908836/1‑150 (MQ=255)

                                                                                                            gCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCg                                        >  2:4656475/1‑150 (MQ=255)

                                                                                                              tCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGaa                                      <  2:1811193/150‑1 (MQ=255)

                                                                                                              tCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGaa                                      >  1:5151124/1‑150 (MQ=255)

                                                                                                              tCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAAGTTCGCGGCCTCAGCGac                                      >  2:7010061/1‑149 (MQ=255)

                                                                                                                cTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGc                                    >  1:4745840/1‑150 (MQ=255)

                                                                                                                  aCA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTAGCCGCGAAGAGGCCGGcgca                                                                                                                                  >  1:4486071/1‑55 (MQ=18)

                                                                                                                  aCA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCaa                                  >  2:1948187/1‑150 (MQ=255)

                                                                                                                   cA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCaaa                                 <  2:5086308/150‑1 (MQ=255)

                                                                                                                   cA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCaaa                                 >  1:3265700/1‑150 (MQ=255)

                                                                                                                    a‑aCGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAAc                                >  1:1967762/1‑150 (MQ=255)

                                                                                                                     accgtttATTGTCCGACTGTTGGTGCGGTTTTACCCGCTAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAAc                                <  1:2587956/145‑1 (MQ=255)

                                                                                                                        ggTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGca                             >  2:6877261/1‑150 (MQ=255)

                                                                                                                         gTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGcaa                            <  1:2415603/150‑1 (MQ=255)

                                                                                                                          ttAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGcaac                           >  2:4709877/1‑150 (MQ=255)

                                                                                                                          ttAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGcaac                           >  1:4859995/1‑150 (MQ=255)

                                                                                                                            attGTCCGCTTGTCGGCGGGGGTTCACCCGCTATGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGcaacaa                         <  1:6232851/148‑1 (MQ=255)

                                                                                                                            aaTGTCCGACTGTAGGATCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGcaacaa                         <  2:4502533/150‑1 (MQ=255)

                                                                                                                                tCCGACTGTTGGAGCGGTTTTACCCGCTACGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCaa                     <  1:3429867/150‑1 (MQ=255)

                                                                                                                                tCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCaa                     <  2:2909684/150‑1 (MQ=255)

                                                                                                                                tCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCaa                     >  1:4344843/1‑150 (MQ=255)

                                                                                                                                tCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCaa                     >  2:7066001/1‑150 (MQ=255)

                                                                                                                                 ccGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGTAAATCACATAACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTAAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAAc                    >  2:589246/1‑150 (MQ=255)

                                                                                                                                   gACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAACAt                  >  1:3165364/1‑150 (MQ=255)

                                                                                                                                   gACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAACAt                  >  2:2599095/1‑150 (MQ=255)

                                                                                                                                   gACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAACAt                  >  2:1123051/1‑150 (MQ=255)

                                                                                                                                   gACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAACAt                  >  2:118506/1‑150 (MQ=255)

                                                                                                                                      tGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAACATcgc               >  2:5965979/1‑150 (MQ=255)

                                                                                                                                        tcttcgCGGGTAAACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAACCTCGCGa             >  2:1331708/6‑150 (MQ=255)

                                                                                                                                        tAGGAGCGGTTTTACCCGCGTTGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAACATCGCGa             <  1:593029/150‑1 (MQ=255)

                                                                                                                                        tAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAACATCGCGa             >  2:3432064/1‑150 (MQ=255)

                                                                                                                                        tAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAACAAACGCAACAAGCAACATCGCGa             >  1:1432069/1‑150 (MQ=255)

                                                                                                                                          ttcgCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAACATCGCGAgg           >  1:6868122/4‑150 (MQ=255)

                                                                                                                                          ttcgCGGGTAAACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAACATCGCGAgg           >  2:4434333/4‑150 (MQ=255)

                                                                                                                                          ggtgCGGGTTTACCCGCTAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAACATCGCGAgg           <  1:7023832/147‑1 (MQ=255)

                                                                                                                                          ggAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAACATCGCGAgg           <  2:6353504/150‑1 (MQ=255)

                                                                                                                                          ggAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAACATCGCGAgg           <  2:6713560/150‑1 (MQ=255)

                                                                                                                                           gAGGGGTTTTACCCGCTAAGTGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAACATCGCGAggg          <  1:2270803/150‑1 (MQ=255)

                                                                                                                                           gAGCGGGTTTAGCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAACATCGCGAggg          >  1:1055454/1‑150 (MQ=255)

                                                                                                                                             gCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTaa                                                         <  1:377748/101‑1 (MQ=255)

                                                                                                                                               gggTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAACATCGCGAGGGTGAt      >  2:4984074/1‑150 (MQ=255)

                                                                                                                                                ggTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAACATCGCGAGGGTGAtt     <  1:4298165/150‑1 (MQ=255)

                                                                                                                                                ggTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAACATCGCGAGGGTGAtt     <  1:7388795/150‑1 (MQ=255)

                                                                                                                                                ggTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAACATCGCGAGGGTGAtt     <  2:3318629/150‑1 (MQ=255)

                                                                                                                                                ggTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAACATCGCGAGGGTGAtt     >  2:1982520/1‑150 (MQ=255)

                                                                                                                                                   ttACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAACATCGCGAGGGTGATTAGc  <  1:5575439/150‑1 (MQ=255)

                                                                                                                                                   ttACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAACATCGCGAGGGTGATTAGc  <  1:6692597/150‑1 (MQ=255)

                                                                                                                                                      cccGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGc                                                             >  2:2525622/1‑88 (MQ=255)

GCATTTGTCACCGGCCAGGAGTTTGTGGTCGATGGCGGCATGACCCGCAAGATGATCTATACCTGAGCACCTTGTTTAAACTGCGCCGGCCTCTTCGCGGCTAAACCCGCTCCTACA‑ACGGTTAATGTCCGACTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATCACATCACGTTGCCTTTTTG‑AAAAAAATTCAATGGGGCTATTGACTTAGCTTCGCCACCTGCGTAAATTTCGCGGCCTCAGCGAAGCAAACGCAACAAGCAACATCGCGAGGGTGATTAGC  >  NC_002947/2276106‑2276401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`