BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,407,303	A→T	5.8%	L19Q (CTG→CAG)	PP_2103 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,407,303	0	A	T	5.8%	1681.3 / 55.4	488	L19Q (CTG→CAG)	PP_2103	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (232/227); new base T (16/12); total (248/240)
Fisher's exact test for biased strand distribution p-value = 5.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 501 total aligned reads displayed.

CGGCACCGATGCCCCACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACACCGCCGCCTTCAGGCGCACGCCGC  >  NC_002947/2407154‑2407452

  gCACCGATGCCCCACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCagcagc                                                                                                                                                     <  1:3150070/150‑1 (MQ=255)

  gCACCGATGCCCCACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCagcagc                                                                                                                                                     >  2:4510124/1‑150 (MQ=255)

  gCACCGATGCCCCACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCAGGCGC‑AGGCGCagcagc                                                                                                                                                     <  2:4148081/150‑1 (MQ=255)

      cGATGCCCCACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCt                                                                                                                                                 <  1:2962685/150‑1 (MQ=255)

       gATGCCCCACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCtt                                                                                                                                                <  1:4113288/150‑1 (MQ=255)

        aTGCCCCACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTg                                                                                                                                               <  2:1866035/150‑1 (MQ=255)

        aTGCCCCACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTg                                                                                                                                               <  2:4871436/150‑1 (MQ=255)

         tGCCCCACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTgc                                                                                                                                              <  2:3884229/150‑1 (MQ=255)

           ccccACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTgcgc                                                                                                                                            >  2:2676442/1‑150 (MQ=255)

            cccACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCa                                                                                                                                           >  2:6731603/1‑150 (MQ=255)

            cccACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCa                                                                                                                                           <  1:3301104/150‑1 (MQ=255)

            cccACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCa                                                                                                                                           >  1:6600046/1‑150 (MQ=255)

             ccACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGCAAGGCGCTGCTGCGCC‑TGCGCat                                                                                                                                          <  2:6348660/150‑2 (MQ=255)

             ccACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAg                                                                                                                                          >  1:6756496/1‑150 (MQ=255)

             ccACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGCTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAg                                                                                                                                          >  1:5640292/1‑150 (MQ=255)

             ccACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGATGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAg                                                                                                                                          >  1:6078686/1‑150 (MQ=255)

              cacaGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGCAAGGCGCTGCTGCGCC‑TGCGCatg                                                                                                                                         <  1:1187745/150‑3 (MQ=255)

                caGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGCAAGGCGCTGCTGCGCC‑TGCGCatgga                                                                                                                                       <  1:5479975/150‑5 (MQ=255)

                  gTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTcgc                                                                                                                                     >  2:6633582/1‑150 (MQ=255)

                   tGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTcgcg                                                                                                                                    <  1:4438662/150‑1 (MQ=255)

                    ggCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTcgcgc                                                                                                                                   <  1:1447120/150‑1 (MQ=255)

                     gcgcATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTcgcgcg                                                                                                                                  <  2:544843/150‑1 (MQ=255)

                      cgcATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCgg                                                                                                                                 <  1:5316816/150‑1 (MQ=255)

                        cATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGt                                                                                                                               >  2:5902180/1‑150 (MQ=255)

                             ttGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTAcgc                                                                                                                          >  2:369614/1‑150 (MQ=255)

                             ttGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTAcgc                                                                                                                          >  1:402991/1‑150 (MQ=255)

                             ttGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTAcgc                                                                                                                          >  2:1777793/1‑150 (MQ=255)

                              tGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTAcgcg                                                                                                                         >  2:1328239/1‑150 (MQ=255)

                                 tACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCgtgt                                                                                                                      <  1:2719788/150‑1 (MQ=255)

                                   cccAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGttt                                                                                                                    <  1:5378720/150‑1 (MQ=255)

                                   cccAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGAGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGttt                                                                                                                    >  1:3874944/1‑150 (MQ=255)

                                    ccAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCCGCAGCCCCTTTCGCAGTTCCCGCCGGTTAAGCGTGTTTg                                                                                                                   >  1:4688202/1‑150 (MQ=255)

                                    ccAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGtt                                                                                                                     <  2:3025316/148‑1 (MQ=255)

                                    ccAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTg                                                                                                                   >  2:2616687/1‑150 (MQ=255)

                                    ccAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTg                                                                                                                   >  1:3765105/1‑150 (MQ=255)

                                     cAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTCCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTgg                                                                                                                  >  2:3383886/1‑150 (MQ=255)

                                       ggCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGGTGTTCATTTCCATGCTC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCa                                                                                                                <  1:1301716/150‑1 (MQ=255)

                                            tCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCt                                                                                                           <  1:28127/150‑1 (MQ=255)

                                              aTGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTgg                                                                                                         >  1:3507863/1‑150 (MQ=255)

                                                  aGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAgg                                                                                                     <  2:6782377/150‑1 (MQ=255)

                                                     gAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTc                                                                                                  <  1:3668160/150‑1 (MQ=255)

                                                        cTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATg                                                                                               <  2:168244/150‑1 (MQ=255)

                                                        cTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATg                                                                                               >  2:74696/1‑150 (MQ=255)

                                                           cccATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCa                                                                                            >  1:3039906/1‑150 (MQ=255)

                                                           cccATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCa                                                                                            >  2:3124076/1‑150 (MQ=255)

                                                             cATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAAc                                                                                          >  1:3427195/1‑150 (MQ=255)

                                                                  cgcgCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTCCGCGTGTTTg                                                                                                                   <  1:2620165/120‑1 (MQ=255)

                                                                  cgcgCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGgcg                                                                                     >  1:2667919/1‑150 (MQ=255)

                                                                  cgcgCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGgcg                                                                                     >  1:4486157/1‑150 (MQ=255)

                                                                   gcgcACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGgcgc                                                                                    <  2:3313965/150‑1 (MQ=255)

                                                                    cgcACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCt                                                                                   >  1:4222605/1‑150 (MQ=255)

                                                                              gcAGCTGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCTGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGATCATGGCAACAGGCGCTCCCTGTCCtt                                                                         <  2:2380583/150‑1 (MQ=255)

                                                                              gcAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCtt                                                                         >  2:5149000/1‑150 (MQ=255)

                                                                              gcAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCtt                                                                         <  1:2496797/150‑1 (MQ=255)

                                                                              gcAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCtt                                                                         <  2:1439899/150‑1 (MQ=255)

                                                                              gcAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCtt                                                                         <  1:5071708/150‑1 (MQ=255)

                                                                               cAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTg                                                                        <  1:1000632/150‑1 (MQ=255)

                                                                               cAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTg                                                                        <  1:743918/150‑1 (MQ=255)

                                                                                aGCGGTTCAAGCAACTGACCTGACTCATGACGGATGTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTccaggg                                                                       <  2:3449379/150‑5 (MQ=255)

                                                                                  cGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCa                                                                     <  1:3935796/150‑1 (MQ=255)

                                                                                    gTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGAGC‑AGGCGCAGCAGCGACTCGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTAGGCAAg                                                                   >  1:1963580/1‑150 (MQ=255)

                                                                                       cAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTtc                                                                >  1:6526269/1‑150 (MQ=255)

                                                                                        aaGCAACTGACCCGACTCATGACGGATATCCTGTCGGTGAATTTCCATGCGC‑ACCCGCAGCAGCGCCTTGCGCAGATCGCGCGCCTTACGCGTGATTGGCAGATCTGGCATCCTCATCCCAACAGGCGCTCCAAGACCTTGGCAATTtct                                                               >  1:5913960/1‑149 (MQ=255)

                                                                                          gCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTtcgtc                                                             >  1:2119415/1‑150 (MQ=255)

                                                                                          gCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTtcgtc                                                             >  2:4951302/1‑150 (MQ=255)

                                                                                            aaCTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGa                                                           <  2:2872052/150‑1 (MQ=255)

                                                                                             aCTGACCGGACTCATGACGGATTTCCTGGCGGTGCAGTTCCATGCGC‑AGGCGCAGCAGCGCCGTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAg                                                          <  2:7170845/150‑1 (MQ=255)

                                                                                               tGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAggg                                                        >  2:7265418/1‑150 (MQ=255)

                                                                                               tGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAg                                                          <  1:2257793/148‑1 (MQ=255)

                                                                                                 aCCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTg                                                      >  2:6549542/1‑150 (MQ=255)

                                                                                                    ggACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGgcg                                                   >  2:255455/1‑150 (MQ=255)

                                                                                                     gACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTc                                                                            >  2:4755333/1‑124 (MQ=255)

                                                                                                     gACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGgcgc                                                  <  2:6225072/150‑1 (MQ=255)

                                                                                                          ctGACGGATGTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAg                                             <  2:4296767/149‑1 (MQ=255)

                                                                                                          aTGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTTGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAg                                             >  2:5528977/1‑150 (MQ=255)

                                                                                                          aTGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAg                                             >  2:3488696/1‑150 (MQ=255)

                                                                                                          aTGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAg                                             <  1:7115499/150‑1 (MQ=255)

                                                                                                          aTGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAg                                             >  1:475705/1‑150 (MQ=255)

                                                                                                            gACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAggg                                           <  2:3784454/150‑1 (MQ=255)

                                                                                                             aCGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGc                                          <  1:1777793/150‑1 (MQ=255)

                                                                                                               ggATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCga                                        >  1:7360382/1‑150 (MQ=255)

                                                                                                                gATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCgat                                       <  1:7265418/150‑1 (MQ=255)

                                                                                                                  tttCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCgatga                                     <  2:1212338/150‑1 (MQ=255)

                                                                                                                     ccTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATtc                                  <  1:6646709/150‑1 (MQ=255)

                                                                                                                       tGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATtcgt                                <  2:2142677/150‑1 (MQ=255)

                                                                                                                        ggCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATtcgtc                               <  1:2375883/150‑1 (MQ=255)

                                                                                                                        ggCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATtcgtc                               <  1:582385/150‑1 (MQ=255)

                                                                                                                        ggCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCGGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATtcgtc                               <  2:5640292/150‑1 (MQ=255)

                                                                                                                           ggTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGaa                            >  1:6952656/1‑150 (MQ=255)

                                                                                                                              gCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAacac                         >  1:895116/1‑150 (MQ=255)

                                                                                                                               cATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTc                                                                                  >  2:594425/1‑92 (MQ=255)

                                                                                                                                aTTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACAccg                       >  1:1931444/1‑150 (MQ=255)

                                                                                                                                   gaactGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACAccgccg                    >  2:5257187/5‑150 (MQ=255)

                                                                                                                                      ctGCGCAAGGCGCTGCTGCGCC‑TGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTtcgt                                                              >  1:6332419/2‑105 (MQ=38)

                                                                                                                                      aTGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCgat                                       <  1:6480527/128‑1 (MQ=255)

                                                                                                                                        gcgc‑aggcgcagCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACACCGCCGCCTTc               <  2:1583047/150‑1 (MQ=255)

                                                                                                                                         cgc‑aggcgcagCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACACCGCCGCCTTCa              <  1:4999079/150‑1 (MQ=255)

                                                                                                                                                cgcagCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACACCGCCGCCTTCAGGCGCa        >  1:5569911/1‑150 (MQ=255)

                                                                                                                                                 gcagcaGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCCAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACACCACCGCCTTCAGGCGCAc       >  2:707413/1‑150 (MQ=255)

                                                                                                                                                     cagcGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGa                                                           >  2:5335128/1‑94 (MQ=255)

CGGCACCGATGCCCCACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACACCGCCGCCTTCAGGCGCACGCCGC  >  NC_002947/2407154‑2407452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`