BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,698,795	G→A	5.0%	A32T (GCC→ACC)	PP_2362 →	usher protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,698,795	0	G	A	5.0%	919.3 / 23.7	293	A32T (GCC→ACC)	PP_2362	usher protein
Reads supporting (aligned to +/- strand): ref base G (146/129); new base A (4/10); total (150/143)
Fisher's exact test for biased strand distribution p-value = 9.96e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.58e-01

Only 100 of 298 total aligned reads displayed.

TCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCAGCAGCGGGCCG  >  NC_002947/2698646‑2698942

tCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTgg                                                                                                                                                     <  1:6906078/150‑1 (MQ=255)

  aaTGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGcc                                                                                                                                                   >  1:6365665/1‑150 (MQ=255)

  aaTGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGcc                                                                                                                                                   <  2:5566113/150‑1 (MQ=255)

  aaTGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGcc                                                                                                                                                   >  1:7090370/1‑150 (MQ=255)

  aaTGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGcc                                                                                                                                                   >  1:7033230/1‑150 (MQ=255)

     ggTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATg                                                                                                                                                <  1:6433185/150‑1 (MQ=255)

     ggTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATg                                                                                                                                                <  1:5848334/150‑1 (MQ=255)

      gTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATgg                                                                                                                                               <  1:5072154/150‑1 (MQ=255)

      gTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATgg                                                                                                                                               <  2:6804805/150‑1 (MQ=255)

       tCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGt                                                                                                                                              <  1:138803/150‑1 (MQ=255)

         aaCACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCa                                                                                                                                            >  1:6680400/1‑150 (MQ=255)

          acacTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAg                                                                                                                                           >  2:283898/1‑150 (MQ=255)

           cacTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGc                                                                                                                                          <  1:1591246/150‑1 (MQ=255)

            acTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGcc                                                                                                                                         <  1:6045212/150‑1 (MQ=255)

            acTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGcc                                                                                                                                         >  2:6210792/1‑150 (MQ=255)

            acTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGcc                                                                                                                                         >  2:6310655/1‑150 (MQ=255)

            acTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGGGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGcc                                                                                                                                         >  2:2861518/1‑150 (MQ=255)

             cTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGccc                                                                                                                                        >  1:4382482/1‑150 (MQ=255)

             cTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGACCATGGCCAGccc                                                                                                                                        <  2:4178938/150‑1 (MQ=255)

                 gcggACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCCGGGGCTGACCATGGCCAGccccagg                                                                                                                                    <  1:770826/150‑4 (MQ=255)

                    gACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCCGGGGCTGACCATGGCCAGccccaggcac                                                                                                                                 <  1:1011688/150‑7 (MQ=255)

                      cGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGt                                                                                                                               >  1:3553324/1‑150 (MQ=255)

                       gCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGGGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTa                                                                                                                              <  2:4914871/150‑1 (MQ=255)

                         cATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACt                                                                                                                            >  2:1556363/1‑150 (MQ=255)

                         cATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACt                                                                                                                            >  2:4423140/1‑150 (MQ=255)

                         cATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATCGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACt                                                                                                                            >  1:7126560/1‑150 (MQ=255)

                           tCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTgg                                                                                                                          <  2:2012757/150‑1 (MQ=255)

                            ccGCCACGGCCAATGCGCCAAGGGCCGAATGAAGTGCTGGGGCCTGGGAGGACCCGGCATTGGTTTGAACCCGTGTGTGAACTGGCTCCCGGCGACGCCGTGCGGCGGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGc                                                                                                                         <  2:4536800/150‑1 (MQ=255)

                            ccGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGc                                                                                                                         <  2:3609615/150‑1 (MQ=255)

                            ccGCCAAGGCCAATCAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGTAGGACCCGGCAATGGTTTGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCAGGCCATGGTCACCCCCGCGCTGGTACTCGc                                                                                                                         <  2:6390832/150‑1 (MQ=255)

                              gacAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTg                                                                                                                                 <  1:3232812/138‑1 (MQ=255)

                               ccAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCcga                                                                                                                      >  2:5198031/1‑150 (MQ=255)

                                cAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCcgac                                                                                                                     >  1:2334371/1‑150 (MQ=255)

                                cAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCcgac                                                                                                                     >  1:2464665/1‑150 (MQ=255)

                                  aGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCACTGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCcgacga                                                                                                                   <  2:6474606/150‑1 (MQ=255)

                                  aGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCcgacga                                                                                                                   >  2:2749076/1‑150 (MQ=255)

                                   ggCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGTCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGACTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCgacgac                                                                                                                  >  2:5905960/1‑150 (MQ=255)

                                     caaaTGAGCCAACGGCCGAATGAAGGGGTGGGGCCAGGGTGGACCGGGATTTGGTTGGAAACGGTGTGTTAAATGGCGCCCGGCGACGACGTGCCGGTGGGGCGTGGGGCTGGCCTTGGACAGCCCCGCGCTGGTACTGGCCGACGACtt                                                                                                                <  1:5015192/148‑1 (MQ=255)

                                        aTGAGCCAACGGCCGAAGGACGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCGCCCGGCGACGCCGTGCCGCGGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGcc                                                                                                             <  2:3752280/150‑1 (MQ=255)

                                            gCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCa                                                                                                         <  2:6044639/150‑1 (MQ=255)

                                                     cGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACg                                                                                                >  2:5441457/1‑150 (MQ=255)

                                                      gAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTCCTGGCCGACGACTTGCCCCCACCGCCCACgg                                                                                               >  2:6502800/1‑150 (MQ=255)

                                                       aaTGATGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAATTGGCTCCCGGCGACGACGTGCCGCTGGTGCGTGGGGCTGGCAAGGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGga                                                                                              <  2:4127974/150‑1 (MQ=255)

                                                       aaTGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGga                                                                                              >  1:3850001/1‑150 (MQ=255)

                                                       aaTGAAGGGCTGGGGCCTGGGAGGACCCGGCATTGTTTGTAAACGGTGTGTGAATTGGCTCCCGGCGACGACGTGCCGGTGGTGCCTGGGGCTGGCCATGGCCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGga                                                                                              <  1:6572669/150‑1 (MQ=255)

                                                         tGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGgaga                                                                                            >  1:6255976/1‑150 (MQ=255)

                                                         tGAAGGGCAGGGGCCTGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGgaga                                                                                            <  2:216026/150‑1 (MQ=255)

                                                             gTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTc                                                                                        >  1:4628820/1‑150 (MQ=255)

                                                                cAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGCCGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGc                                                                                     <  2:2381467/150‑1 (MQ=255)

                                                                         gggAGGACCCGGCATTGGTTGGAACCCGTGTGTGAACTGGCTCCCGGCGCCGCCGTGCCGGGGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGa                                                                            <  1:1025766/150‑1 (MQ=255)

                                                                              gaCCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCa                                                                       >  1:4393350/1‑150 (MQ=255)

                                                                                cccGGCTTTGTTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGCCGTGCCGCTGGTGCCTGGGGCGGGCAATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCAcc                                                                     <  1:7298915/150‑1 (MQ=255)

                                                                                 ccGGCATTGGTTGGCAACCGTGTGGGACAGGGCTCCCTGCGAGGAGGGGCGGCTGGTGCCGGGGGCTGGCCATGGCCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCAccc                                                                    <  1:2184518/150‑1 (MQ=255)

                                                                                 ccGGAATTGGTTGGAAACGTTTTGTTAATTGGCTCCCGGCGACGACGTGCCGGTGGTGCCTGGGGCGGGCCTTGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCAccc                                                                    <  1:6286701/150‑1 (MQ=255)

                                                                                   ggCAATGGTTGGAACCCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCtt                                                                  <  2:1042987/150‑1 (MQ=255)

                                                                                    gCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCttt                                                                 >  1:5606414/1‑150 (MQ=255)

                                                                                      aaTGGTTGGAAACAGTGTGAGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGACATCGACGAGGCCACCCTTTAc                                                               >  2:6596112/1‑150 (MQ=255)

                                                                                          gTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCg                                                           >  2:169818/1‑150 (MQ=255)

                                                                                          gTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCg                                                           >  1:392016/1‑150 (MQ=255)

                                                                                           ttGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGa                                                          >  2:3817239/1‑150 (MQ=255)

                                                                                           gtGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGa                                                          >  2:1431778/2‑150 (MQ=255)

                                                                                              gAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCt                                                       >  2:3239690/1‑150 (MQ=255)

                                                                                                aaCCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCtgg                                                     <  2:7029430/150‑1 (MQ=255)

                                                                                                aaCCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCtgg                                                     >  2:3487117/1‑150 (MQ=255)

                                                                                                 aCCGGGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCtggt                                                    <  2:3872360/150‑1 (MQ=255)

                                                                                                  ccGTGTGTGACCTGGCTCCCGGCGACGACGTGCCGCCGGTGCCTGGGGCCGGCCAGGGACAGCCCCGCGCTGGTACTGGCCGACGACGGGCCCCCACCGCCCACCGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCtggtg                                                   <  2:291516/150‑1 (MQ=255)

                                                                                                  ccGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCtggtg                                                   <  2:3653076/150‑1 (MQ=255)

                                                                                                    gtgtgtGAACTGGCTCCCGGCGACGAGGGGCCGCTGGGGCGTGGGGCTGGCCTGGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTggtggt                                                 <  1:6471047/150‑1 (MQ=255)

                                                                                                    gtgtgtGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTggtggt                                                 <  2:5724905/150‑1 (MQ=255)

                                                                                                       tgtgAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGaa                                              >  2:3219494/1‑150 (MQ=255)

                                                                                                       tgtgAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGaa                                              >  1:2087384/1‑150 (MQ=255)

                                                                                                         tgAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAAcc                                            >  1:5811708/1‑150 (MQ=255)

                                                                                                                  tCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGcccc                                   >  1:199743/1‑149 (MQ=255)

                                                                                                                  tCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCa                                   <  2:7345926/150‑1 (MQ=255)

                                                                                                                  tCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCa                                   >  1:6134810/1‑150 (MQ=255)

                                                                                                                    ccGGCGACGCCGGGCGGCTGGTGCGTGGGGCGGGCCCGGGCCCGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAgg                                 <  1:2930391/150‑1 (MQ=255)

                                                                                                                    ccGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAgg                                 >  1:1654179/1‑150 (MQ=255)

                                                                                                                     cGGCGACGACGTGCCGGTGGTGCCTGGGGCTGGCCAGGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAggg                                <  1:361282/150‑1 (MQ=255)

                                                                                                                     cGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAggg                                >  2:1898053/1‑150 (MQ=255)

                                                                                                                     cGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAggg                                >  2:5993809/1‑150 (MQ=255)

                                                                                                                            gacgTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCt                         >  1:1373728/1‑150 (MQ=255)

                                                                                                                            gacgTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCt                         >  1:4175625/1‑150 (MQ=255)

                                                                                                                              cgTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGa                       <  2:7033230/150‑1 (MQ=255)

                                                                                                                               gTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGAt                      >  1:114942/1‑150 (MQ=255)

                                                                                                                                 gCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATAc                    <  2:7090370/150‑1 (MQ=255)

                                                                                                                                   cggtGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCg                  <  1:5969906/147‑1 (MQ=255)

                                                                                                                                   cGCTGGTGCGTGGGGCTGGCCATGGTCCGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCg                  <  1:1200285/150‑1 (MQ=255)

                                                                                                                                    gCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCgg                 >  2:2694577/1‑150 (MQ=255)

                                                                                                                                      tGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTg               <  1:2861473/150‑1 (MQ=255)

                                                                                                                                        gggCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTgca             <  1:4848545/148‑1 (MQ=255)

                                                                                                                                        gTGCCTGGGGCTGGCCATGGTCCCCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTgca             <  1:5316308/150‑1 (MQ=255)

                                                                                                                                        gTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTgca             >  1:4055239/1‑150 (MQ=255)

                                                                                                                                           ccTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTgcagca          >  1:3390880/1‑150 (MQ=255)

                                                                                                                                           ccTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGCCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTgcagca          >  1:2706217/1‑150 (MQ=255)

                                                                                                                                             gggggggggCCTTGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCagcagc        <  1:4504126/143‑1 (MQ=255)

                                                                                                                                               gggCTGGCCATGGCCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCAGCAGcgg      <  1:5535031/150‑1 (MQ=255)

                                                                                                                                                 gCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCAGCAGcgggc    >  2:4995173/1‑150 (MQ=255)

                                                                                                                                                   tGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCAGCAGcgggccg  >  1:1978116/1‑150 (MQ=255)

                                                                                                                                                   tGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCAGCAGcgggccg  >  1:5412037/1‑150 (MQ=255)

                                                                                                                                                   tGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCAGCAGcgggccg  >  1:2983187/1‑150 (MQ=255)

TCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCAGCAGCGGGCCG  >  NC_002947/2698646‑2698942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`