BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,813,538	A→T	6.1%	noncoding (414/434 nt)	PP_mr35 ←	cspA regulatory region

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,813,538	0	A	T	6.1%	863.8 / 29.2	285	noncoding (414/434 nt)	PP_mr35	cspA regulatory region
Reads supporting (aligned to +/- strand): ref base A (146/113); new base T (9/9); total (159/126)
Fisher's exact test for biased strand distribution p-value = 6.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 297 total aligned reads displayed.

CGCAAAATGTGAAAAGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGATGGCTTTGAAGTGTA  >  NC_002947/2813390‑2813685

   aaaaTGTGAAAAGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAggg                                                                                                                                                 >  2:322954/1‑150 (MQ=255)

    aaaTGTGAAAAGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGc                                                                                                                                                >  2:6605163/1‑150 (MQ=255)

      aTGTGAAAAGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCgg                                                                                                                                              <  2:157664/150‑1 (MQ=255)

       tgtgAAAAGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGACCACGGGCAAAGCCCGTGCCCTTCACCCGACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCggg                                                                                                                                             <  2:6809749/150‑1 (MQ=255)

          gAAAAGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGttt                                                                                                                                          <  2:6224952/150‑1 (MQ=255)

          gAAAAGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGttt                                                                                                                                          <  1:4029109/150‑1 (MQ=255)

           aaaaGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGtttt                                                                                                                                         >  1:1971921/1‑150 (MQ=255)

           aaaaGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGtttt                                                                                                                                         <  1:6479650/150‑1 (MQ=255)

            aaaGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCCTTGTGGCGGGttttt                                                                                                                                        <  2:2881907/150‑1 (MQ=39)

             aaGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGtttttt                                                                                                                                       <  1:5482047/150‑1 (MQ=255)

             aaGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGtttttt                                                                                                                                       >  1:6618262/1‑150 (MQ=255)

              aGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGttttttt                                                                                                                                      <  1:6968766/150‑1 (MQ=255)

              aGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGttttttg                                                                                                                                      <  1:33119/150‑2 (MQ=255)

                 cTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACa                                                                                                                                   <  1:4267859/150‑1 (MQ=255)

                 cTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACa                                                                                                                                   <  1:2677600/150‑1 (MQ=255)

                  tGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCCCAAGGGCGGGTTTTTTTACAg                                                                                                                                  >  2:2948155/1‑150 (MQ=255)

                   gcgcGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGc                                                                                                                                 >  2:1461906/1‑150 (MQ=255)

                       ggTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATc                                                                                                                             >  1:1017498/1‑150 (MQ=255)

                           gCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGaa                                                                                                                         <  2:636457/150‑1 (MQ=255)

                             cTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACGAGGGCGGGTTTTTTTACAGCTATCCGACtt                                                                                                                       >  1:3630856/1‑150 (MQ=255)

                             cTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAAtt                                                                                                                       >  1:1220307/1‑150 (MQ=255)

                              ttGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTACGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTa                                                                                                                      >  2:7133941/1‑150 (MQ=255)

                               tGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTAc                                                                                                                     <  1:6717167/150‑1 (MQ=255)

                                    gAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGttttt                                                                                                                                        >  1:6890104/1‑126 (MQ=255)

                                      gCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGAc                                                                                                              >  1:5339957/1‑150 (MQ=255)

                                          gTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAAc                                                                                                          <  2:7348135/150‑1 (MQ=255)

                                            tCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCt                                                                                                        >  2:7285642/1‑150 (MQ=255)

                                            tCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCt                                                                                                        <  2:606218/150‑1 (MQ=255)

                                            tCACCCGCGAACACGGGCAAACCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCt                                                                                                        <  2:4927027/150‑1 (MQ=255)

                                                ccGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGc                                                                                                    >  2:3124641/1‑150 (MQ=255)

                                                  gcgAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGtttt                                                                                                                                         <  1:6400385/111‑1 (MQ=255)

                                                  gcgAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAg                                                                                                  >  1:5696943/1‑150 (MQ=255)

                                                     aaCACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCt                                                                                               <  2:2684521/150‑1 (MQ=255)

                                                      gcacGGGCTTTGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATTATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCAGTGCAGCCTg                                                                                              >  1:21809/2‑150 (MQ=255)

                                                       cacGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGc                                                                                             <  2:5063544/150‑1 (MQ=255)

                                                        acGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAg                                                                                                                                  <  1:366185/112‑1 (MQ=255)

                                                        acGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCa                                                                                            <  2:1093610/150‑1 (MQ=255)

                                                        acGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCa                                                                                            >  1:1391812/1‑150 (MQ=255)

                                                        acGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGGTTCGCAGGCAATCCCGCCCCTTCAGATGATGCCTCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCa                                                                                            >  2:6963773/1‑150 (MQ=255)

                                                          gggCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATg                                                                                          >  2:86361/1‑150 (MQ=255)

                                                          gggCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATg                                                                                          >  2:4646984/1‑150 (MQ=255)

                                                          gggCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATg                                                                                          >  2:7425413/1‑150 (MQ=255)

                                                                aGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCACCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTc                                                                                    <  2:1675366/150‑1 (MQ=255)

                                                                 gCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCt                                                                                   <  1:4780276/150‑1 (MQ=255)

                                                                 gCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCGATCCGACTTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCt                                                                                   <  1:44958/150‑1 (MQ=255)

                                                                   ccGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTgg                                                                                 >  2:1972624/1‑150 (MQ=255)

                                                                         cATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCgcgc                                                                           >  2:2340413/1‑150 (MQ=255)

                                                                          aTTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCt                                                                          >  2:4240940/1‑150 (MQ=255)

                                                                           ttCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGCATTACTCCGGACGAACCGGTGCAGCCTGCATGCCCGTCTGGCCgcgcgc                                                                         >  2:5283283/1‑148 (MQ=255)

                                                                           ttCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTc                                                                         <  1:7390498/150‑1 (MQ=255)

                                                                                ccTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGcga                                                                    <  1:4665897/150‑1 (MQ=255)

                                                                                ccTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGcga                                                                    >  2:4665897/1‑150 (MQ=255)

                                                                                 cTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGcgac                                                                   >  2:5885499/1‑150 (MQ=255)

                                                                                 cTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGcgac                                                                   >  2:4025924/1‑150 (MQ=255)

                                                                                    cAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAACAAACCCGCCACAAGGGCGGGTTTTTTTACAGCGATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGaa                                                                <  2:4502317/150‑1 (MQ=255)

                                                                                     aGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑ACACAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAg                                                               <  2:1815976/150‑1 (MQ=255)

                                                                                       tCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCt                                                                                   >  1:395200/1‑128 (MQ=255)

                                                                                       tCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCt                                                                                   <  2:4114109/128‑1 (MQ=255)

                                                                                         ggTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGaaa                                                           <  2:26483/150‑1 (MQ=255)

                                                                                         ggTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGaaa                                                           <  2:5015580/150‑1 (MQ=255)

                                                                                          gTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAAc                                                          >  1:3875511/1‑150 (MQ=255)

                                                                                           tttCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACa                                                         <  1:654449/150‑1 (MQ=255)

                                                                                             tCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGt                                                       <  1:7076691/150‑1 (MQ=255)

                                                                                              cGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTc                                                      <  1:3528402/150‑1 (MQ=255)

                                                                                               gCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCt                                                     >  2:1036421/1‑150 (MQ=255)

                                                                                               gCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCt                                                     >  1:4318625/1‑150 (MQ=255)

                                                                                               gCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCt                                                     >  2:3416017/1‑150 (MQ=255)

                                                                                                cAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGc                                                                      <  2:6642872/132‑1 (MQ=255)

                                                                                                cAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTg                                                    <  1:2948155/150‑1 (MQ=255)

                                                                                                cAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTg                                                    >  2:3788167/1‑150 (MQ=255)

                                                                                                 aGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGaa                                                                >  2:7394749/1‑138 (MQ=255)

                                                                                                 aGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTgg                                                   >  1:717407/1‑150 (MQ=255)

                                                                                                 aGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTgg                                                   >  1:1701233/1‑150 (MQ=255)

                                                                                                   gCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCGTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGcc                                                 >  2:1954490/1‑150 (MQ=255)

                                                                                                     aaTCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCctt                                               >  1:164281/1‑150 (MQ=255)

                                                                                                         ccGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCttt                                           >  1:4469078/1‑150 (MQ=255)

                                                                                                         ccGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCttt                                           >  2:5649170/1‑150 (MQ=255)

                                                                                                          cGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTc                                          <  1:6310728/150‑1 (MQ=255)

                                                                                                           gCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGATTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCa                                         <  1:6053429/150‑1 (MQ=255)

                                                                                                                ttCAGATGATGCATCACCCAT‑AAAAAAACCCCCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCAGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGctc                                    <  1:2779345/150‑1 (MQ=255)

                                                                                                                 tCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGctct                                   <  1:1041073/150‑1 (MQ=255)

                                                                                                                  cAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGTAACAGTCTGGCCTTCTTTCAGGCTCtt                                  <  1:5500021/150‑1 (MQ=255)

                                                                                                                        atgCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGcc                            <  1:1461906/150‑1 (MQ=255)

                                                                                                                            aTCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCa                        >  1:1682221/1‑150 (MQ=255)

                                                                                                                             tCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAg                       >  1:4100379/1‑150 (MQ=255)

                                                                                                                               aCCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGAt                     >  1:782429/1‑150 (MQ=255)

                                                                                                                                cccAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGAtt                    >  1:3575520/1‑150 (MQ=255)

                                                                                                                                   aT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGa                 >  1:6280410/1‑150 (MQ=255)

                                                                                                                                   aT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGa                 >  1:4188928/1‑150 (MQ=255)

                                                                                                                                   aT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGa                 >  1:2386692/1‑150 (MQ=255)

                                                                                                                                    t‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGAt                <  2:1220307/150‑1 (MQ=255)

                                                                                                                                    t‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGAt                <  1:6865161/150‑1 (MQ=255)

                                                                                                                                      aaaaaaaCCCGCCACACGGGCGGGTTTTTTTACAGCTATCCGACTTACTCCGGACGACCCTGTGCAGTCTGCAGGCCCTTCTGGCCGCGCTCGGCGACGACGGAAACAGTCTGGCCTTCTTTCAGGCGCTTGAAGCCGTCAGATTCGATg               >  2:6508488/1‑150 (MQ=255)

                                                                                                                                          aaaCCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGATGGCtt           >  1:7278889/1‑150 (MQ=255)

                                                                                                                                           aaCCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGATGGCttt          >  1:5282278/1‑150 (MQ=255)

                                                                                                                                             cccGCCACAAGGGCGGGATTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGATGGCTTTGa        <  2:5217775/150‑1 (MQ=255)

                                                                                                                                              ccGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGTCGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGATGGCTTTGaa       <  1:504481/150‑1 (MQ=255)

                                                                                                                                              ccGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGATGGCTTTGaa       <  2:3630856/150‑1 (MQ=255)

                                                                                                                                               cGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGATGGCTTTGAAg      >  1:4890088/1‑150 (MQ=255)

                                                                                                                                                 ccACTAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACATTCTGGCCTTCTTTCAGGATCTTGAAGCCGTCAGATTCGATGGCTTTGAAgtg    <  1:1070717/150‑1 (MQ=255)

CGCAAAATGTGAAAAGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGATGGCTTTGAAGTGTA  >  NC_002947/2813390‑2813685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`