BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,813,540	G→T	5.6%	noncoding (412/434 nt)	PP_mr35 ←	cspA regulatory region

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,813,540	0	G	T	5.6%	964.2 / 31.0	293	noncoding (412/434 nt)	PP_mr35	cspA regulatory region
Reads supporting (aligned to +/- strand): ref base G (152/123); new base T (9/8); total (162/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

Only 100 of 299 total aligned reads displayed.

GCAAAATGTGAAAAGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGATGGCTTTGAAGTGTACGAA  >  NC_002947/2813391‑2813689

  aaaaTGTGAAAAGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAggg                                                                                                                                                     >  2:322954/1‑150 (MQ=255)

   aaaTGTGAAAAGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGc                                                                                                                                                    >  2:6605163/1‑150 (MQ=255)

     aTGTGAAAAGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCgg                                                                                                                                                  <  2:157664/150‑1 (MQ=255)

      tgtgAAAAGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGACCACGGGCAAAGCCCGTGCCCTTCACCCGACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCggg                                                                                                                                                 <  2:6809749/150‑1 (MQ=255)

         gAAAAGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGttt                                                                                                                                              <  2:6224952/150‑1 (MQ=255)

          aaaaGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AACACAACCCGCCACAAGGGCGGGtttt                                                                                                                                             <  1:2905175/150‑1 (MQ=255)

          aaaaGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGtttt                                                                                                                                             <  1:6479650/150‑1 (MQ=255)

          aaaaGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGtttt                                                                                                                                             >  1:1971921/1‑150 (MQ=255)

           aaaGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCCTTGTGGCGGGttttt                                                                                                                                            <  2:2881907/150‑1 (MQ=39)

            aaGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGtttttt                                                                                                                                           <  1:5482047/150‑1 (MQ=255)

             aGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGttttttt                                                                                                                                          <  1:6968766/150‑1 (MQ=255)

               ccTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCCTTGTGGCGGGTTTTTTTat                                                                                                                                        <  1:665764/150‑2 (MQ=39)

                 tGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCCCAAGGGCGGGTTTTTTTACAg                                                                                                                                      >  2:2948155/1‑150 (MQ=255)

                  gcgcGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGc                                                                                                                                     >  2:1461906/1‑150 (MQ=255)

                      ggTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATc                                                                                                                                 >  1:1017498/1‑150 (MQ=255)

                          gCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGaa                                                                                                                             >  1:4410340/1‑150 (MQ=255)

                          gCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGaa                                                                                                                             <  2:636457/150‑1 (MQ=255)

                            cTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAAtt                                                                                                                           >  1:1220307/1‑150 (MQ=255)

                             ttGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTACGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTa                                                                                                                          >  2:7133941/1‑150 (MQ=255)

                                   gAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGttttt                                                                                                                                            >  1:6890104/1‑126 (MQ=255)

                                   gAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCgg                                                                                                                    >  1:2742784/1‑150 (MQ=255)

                                     gCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGAc                                                                                                                  >  1:5339957/1‑150 (MQ=255)

                                         gTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAAc                                                                                                              <  2:7348135/150‑1 (MQ=255)

                                           tCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCt                                                                                                            <  2:606218/150‑1 (MQ=255)

                                           tCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCt                                                                                                            >  2:7285642/1‑150 (MQ=255)

                                           tCACCCGCGAACACGGGCAAACCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCt                                                                                                            <  2:4927027/150‑1 (MQ=255)

                                               ccGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGc                                                                                                        >  2:3124641/1‑150 (MQ=255)

                                                 gcgAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGtttt                                                                                                                                             <  1:6400385/111‑1 (MQ=255)

                                                 gcgAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAg                                                                                                      >  1:5696943/1‑150 (MQ=255)

                                                    aaCACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCt                                                                                                   <  2:2684521/150‑1 (MQ=255)

                                                     gcacGGGCTTTGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATTATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCAGTGCAGCCTg                                                                                                  >  1:21809/2‑150 (MQ=255)

                                                      cacGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGc                                                                                                 <  2:5063544/150‑1 (MQ=255)

                                                       acGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCa                                                                                                >  1:1391812/1‑150 (MQ=255)

                                                       acGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCa                                                                                                <  2:1093610/150‑1 (MQ=255)

                                                       acGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGGTTCGCAGGCAATCCCGCCCCTTCAGATGATGCCTCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCa                                                                                                >  2:6963773/1‑150 (MQ=255)

                                                         gggCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATg                                                                                              >  2:86361/1‑150 (MQ=255)

                                                         gggCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATg                                                                                              >  2:7425413/1‑150 (MQ=255)

                                                         gggCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATg                                                                                              >  2:4646984/1‑150 (MQ=255)

                                                               aGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCACCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTc                                                                                        <  2:1675366/150‑1 (MQ=255)

                                                                  ccGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTgg                                                                                     >  2:1972624/1‑150 (MQ=255)

                                                                  ccGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTgg                                                                                     <  1:6605163/150‑1 (MQ=255)

                                                                        cATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCgcgc                                                                               >  2:2340413/1‑150 (MQ=255)

                                                                         aTTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCt                                                                              >  2:4240940/1‑150 (MQ=255)

                                                                          ttCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGCATTACTCCGGACGAACCGGTGCAGCCTGCATGCCCGTCTGGCCgcgcgc                                                                             >  2:5283283/1‑148 (MQ=255)

                                                                          ttCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTc                                                                             <  1:7390498/150‑1 (MQ=255)

                                                                               ccTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGcga                                                                        >  1:4079528/1‑150 (MQ=255)

                                                                               ccTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGcga                                                                        >  2:4665897/1‑150 (MQ=255)

                                                                                cTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGcgac                                                                       >  2:5885499/1‑150 (MQ=255)

                                                                                cTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGcgac                                                                       >  2:4025924/1‑150 (MQ=255)

                                                                                  aCAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCGCGGcgacga                                                                     >  1:4231839/1‑150 (MQ=255)

                                                                                   cAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAACAAACCCGCCACAAGGGCGGGTTTTTTTACAGCGATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGaa                                                                    <  2:4502317/150‑1 (MQ=255)

                                                                                    aGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑ACACAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAg                                                                   <  2:1815976/150‑1 (MQ=255)

                                                                                    aGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAg                                                                   >  1:681748/1‑150 (MQ=255)

                                                                                      tCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCt                                                                                       <  2:4114109/128‑1 (MQ=255)

                                                                                        ggTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGaaa                                                               <  2:5015580/150‑1 (MQ=255)

                                                                                        ggTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGaaa                                                               <  2:26483/150‑1 (MQ=255)

                                                                                          tttCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACa                                                             <  1:654449/150‑1 (MQ=255)

                                                                                          tttCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACa                                                             <  1:50056/150‑1 (MQ=255)

                                                                                            tCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGt                                                           <  1:7076691/150‑1 (MQ=255)

                                                                                             cGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTc                                                          <  1:3528300/150‑1 (MQ=255)

                                                                                              gCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCt                                                         >  2:3416017/1‑150 (MQ=255)

                                                                                              gCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCt                                                         >  2:1036421/1‑150 (MQ=255)

                                                                                               cAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGc                                                                          <  2:6642872/132‑1 (MQ=255)

                                                                                               cAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTg                                                        >  2:3788167/1‑150 (MQ=255)

                                                                                                aGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGaa                                                                    >  2:7394749/1‑138 (MQ=255)

                                                                                                aGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTgg                                                       >  1:717407/1‑150 (MQ=255)

                                                                                                aGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTgg                                                       <  1:4646984/150‑1 (MQ=255)

                                                                                                aGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTgg                                                       >  1:1701233/1‑150 (MQ=255)

                                                                                                 ggCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑ACAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGc                                                      <  1:3561755/150‑1 (MQ=255)

                                                                                                  gCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCGTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGcc                                                     >  2:1954490/1‑150 (MQ=255)

                                                                                                    aaTCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCctt                                                   >  1:164281/1‑150 (MQ=255)

                                                                                                        ccGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCttt                                               >  1:3941124/1‑150 (MQ=255)

                                                                                                        ccGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCttt                                               >  2:5649170/1‑150 (MQ=255)

                                                                                                         cGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTc                                              >  1:3789889/1‑150 (MQ=255)

                                                                                                         cGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTc                                              <  1:6310728/150‑1 (MQ=255)

                                                                                                          gCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCa                                             <  1:267755/150‑1 (MQ=255)

                                                                                                          gCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGATTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCa                                             <  1:6053429/150‑1 (MQ=255)

                                                                                                                tCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGctct                                       <  1:1041073/150‑1 (MQ=255)

                                                                                                                 cAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGTAACAGTCTGGCCTTCTTTCAGGCTCtt                                      <  1:5500021/150‑1 (MQ=255)

                                                                                                                       atgCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGcc                                <  1:1461906/150‑1 (MQ=255)

                                                                                                                           aTCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCa                            >  1:1682221/1‑150 (MQ=255)

                                                                                                                              aCCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGAt                         >  1:4495599/1‑150 (MQ=255)

                                                                                                                              aCCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGAt                         >  1:3640767/1‑150 (MQ=255)

                                                                                                                              aCCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGAt                         >  1:782429/1‑150 (MQ=255)

                                                                                                                               cccAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGCATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGAtt                        >  1:3576238/1‑150 (MQ=255)

                                                                                                                                  aT‑AAACAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCGGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGa                     <  1:4309737/150‑1 (MQ=255)

                                                                                                                                  aT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGa                     >  1:2386692/1‑150 (MQ=255)

                                                                                                                                  aT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGa                     >  1:6280410/1‑150 (MQ=255)

                                                                                                                                   t‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGAt                    <  2:1220307/150‑1 (MQ=255)

                                                                                                                                     aaaaaaaCCCGCCACACGGGCGGGTTTTTTTACAGCTATCCGACTTACTCCGGACGACCCTGTGCAGTCTGCAGGCCCTTCTGGCCGCGCTCGGCGACGACGGAAACAGTCTGGCCTTCTTTCAGGCGCTTGAAGCCGTCAGATTCGATg                   >  2:6508488/1‑150 (MQ=255)

                                                                                                                                      aaaaaaCCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGATgg                  >  1:4772892/1‑150 (MQ=255)

                                                                                                                                         aaaCCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGATGGCtt               >  1:7278889/1‑150 (MQ=255)

                                                                                                                                            cccGCCACAAGGGCGGGATTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGATGGCTTTGa            <  2:5217775/150‑1 (MQ=255)

                                                                                                                                             ccGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGATGGCTTTGaa           >  1:5265505/1‑150 (MQ=255)

                                                                                                                                             ccGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGATGGCTTTGaa           <  2:3630856/150‑1 (MQ=255)

                                                                                                                                              cGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGATGGCTTTGAAg          >  1:4139464/1‑150 (MQ=255)

                                                                                                                                              cGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGATGGCTTTGAAg          >  1:4886849/1‑150 (MQ=255)

                                                                                                                                                ccACTAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACATTCTGGCCTTCTTTCAGGATCTTGAAGCCGTCAGATTCGATGGCTTTGAAgtg        <  1:1070717/150‑1 (MQ=255)

                                                                                                                                                 cacaAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGATGGCTTTGAAgtgt       >  1:3165863/1‑150 (MQ=255)

                                                                                                                                                  acaAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGATGGCTTTGAAGTGTa      >  1:4022341/1‑150 (MQ=255)

GCAAAATGTGAAAAGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCAT‑AAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGATGGCTTTGAAGTGTACGAA  >  NC_002947/2813391‑2813689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`