BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,005,757	T→G	5.3%	V77V (GTA→GTC)	PP_2624 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,005,757	0	T	G	5.3%	1277.4 / 40.7	361	V77V (GTA→GTC)	PP_2624	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (139/203); new base G (10/9); total (149/212)
Fisher's exact test for biased strand distribution p-value = 3.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 366 total aligned reads displayed.

TTCAGGGCGACCAGTGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGCTCCACCTC  >  NC_002947/3005608‑3005906

ttCAGGGCGACCAGTGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGt                                                                                                                                                       >  2:5292926/1‑150 (MQ=255)

   aGGGCGACCAGTGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTAcc                                                                                                                                                    <  2:4953439/150‑1 (MQ=255)

   aGGGCGACCAGTGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTAcc                                                                                                                                                    >  2:386075/1‑150 (MQ=255)

          ccAGTGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGt                                                                                                                                             >  1:1812505/1‑150 (MQ=255)

           cAGTGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTc                                                                                                                                            <  1:974940/150‑1 (MQ=255)

              tGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGTGACGGTACCGTCCAg                                                                                                                                         <  1:2106693/150‑1 (MQ=37)

               gCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGTGACGGTACCGTCCAgg                                                                                                                                        <  1:3333016/150‑1 (MQ=37)

               gCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAgg                                                                                                                                        <  1:4873803/150‑1 (MQ=255)

                cTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGTGACGGTACCGTCCAGGc                                                                                                                                       <  1:2936467/150‑1 (MQ=38)

                    gcgcAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTcac                                                                                                                                   >  1:6991609/1‑150 (MQ=255)

                      gcAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACAt                                                                                                                                 >  2:7014494/1‑150 (MQ=255)

                       cAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACAtt                                                                                                                                >  1:4010680/1‑150 (MQ=255)

                        aGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTg                                                                                                                               <  2:1499163/150‑1 (MQ=255)

                          cgcCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGc                                                                                                                             <  1:5912278/150‑1 (MQ=255)

                            ccAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGcgt                                                                                                                           <  2:2487035/150‑1 (MQ=255)

                            ccAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGcgg                                                                                                                           >  1:2585142/1‑149 (MQ=255)

                             cagcagCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGcgtc                                                                                                                          <  2:193200/150‑1 (MQ=255)

                             cagcagCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGcgtc                                                                                                                          <  2:816679/150‑1 (MQ=255)

                                cagcTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGtt                                                                                                                       <  1:547973/150‑1 (MQ=255)

                                cagcTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGtt                                                                                                                       <  1:6055735/150‑1 (MQ=255)

                                 agcTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTc                                                                                                                      <  2:3738967/150‑1 (MQ=255)

                                 agcTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTc                                                                                                                      <  1:1046322/150‑1 (MQ=255)

                                    ttGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAac                                                                                                                   <  1:222811/150‑1 (MQ=255)

                                      gTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAacac                                                                                                                 >  1:1705116/1‑150 (MQ=255)

                                      gTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAacac                                                                                                                 <  2:338550/150‑1 (MQ=255)

                                          aGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTg                                                                                                             >  2:6663077/1‑150 (MQ=255)

                                          aGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTg                                                                                                             >  2:4265529/1‑150 (MQ=255)

                                            cTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGtt                                                                                                           >  2:1144210/1‑150 (MQ=255)

                                            cTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGtt                                                                                                           <  1:7227320/150‑1 (MQ=255)

                                               gggCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCTCGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGaa                                                                                                        <  2:5528615/150‑1 (MQ=255)

                                               gggCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGaa                                                                                                        <  1:216725/150‑1 (MQ=255)

                                                  cACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACAGCGTGTTGaacga                                                                                                     <  1:5334483/150‑4 (MQ=255)

                                                  cACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAatgt                                                                                                     <  1:376566/150‑1 (MQ=255)

                                                   aCCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAatgta                                                                                                    <  1:6673720/150‑1 (MQ=255)

                                                    ccTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAatgtat                                                                                                   <  1:1167875/150‑1 (MQ=255)

                                                     cTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAatgtctg                                                                                                  >  2:6059772/1‑150 (MQ=255)

                                                          gcgcgATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTAt                                                                                             <  1:553918/150‑1 (MQ=255)

                                                          gcgcgATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTAt                                                                                             <  1:3004299/150‑1 (MQ=255)

                                                              gATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGAt                                                                                         >  2:7215139/1‑150 (MQ=255)

                                                              gATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGAt                                                                                         <  1:1448805/150‑1 (MQ=255)

                                                               aTCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATg                                                                                        <  1:4061036/150‑1 (MQ=255)

                                                               aTCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATg                                                                                        <  1:6737628/150‑1 (MQ=255)

                                                                  cggtccggCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATgcgc                                                                                     <  1:2012170/150‑1 (MQ=255)

                                                                  cggtccggCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATgcgc                                                                                     <  2:261912/150‑1 (MQ=255)

                                                                  cggtccggCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATgcgc                                                                                     <  1:3718874/150‑1 (MQ=255)

                                                                   ggtccggCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCa                                                                                    >  1:131846/1‑150 (MQ=255)

                                                                    gtccggCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCAt                                                                                   >  2:3550348/1‑150 (MQ=255)

                                                                            cGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGAc                                                                           <  2:2301060/150‑1 (MQ=255)

                                                                                aTCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTc                                                                       <  2:4003438/150‑1 (MQ=255)

                                                                                aTCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTc                                                                       <  1:2332163/150‑1 (MQ=255)

                                                                                   gTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTc                                                                    >  1:6490551/1‑150 (MQ=255)

                                                                                   gTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTc                                                                    >  2:1765499/1‑150 (MQ=255)

                                                                                    ttGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCa                                                                   >  1:5807778/1‑150 (MQ=255)

                                                                                     tGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCaa                                                                  >  1:7072774/1‑150 (MQ=255)

                                                                                      gATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAg                                                                 <  2:1376715/150‑1 (MQ=255)

                                                                                       gtccaCCTGCAGTTCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAgc                                                                >  1:7367382/5‑150 (MQ=255)

                                                                                       aTGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACCTTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCGGACGGTCCTCAAgc                                                                >  2:5478139/1‑150 (MQ=255)

                                                                                       aTGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAgc                                                                >  2:5928234/1‑150 (MQ=255)

                                                                                       aTGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAgc                                                                >  1:4736949/1‑150 (MQ=255)

                                                                                          gACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCa                                                             <  2:6278504/150‑1 (MQ=255)

                                                                                           aCCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCAc                                                            >  2:1065420/1‑150 (MQ=255)

                                                                                             cTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTg                                                                                                                               >  2:5420896/1‑81 (MQ=255)

                                                                                             cTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACgg                                                          <  2:4406140/150‑1 (MQ=255)

                                                                                              tGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGc                                                         >  2:3105524/1‑150 (MQ=255)

                                                                                               gAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCt                                                        <  1:359539/150‑1 (MQ=255)

                                                                                               gAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCt                                                        <  1:7270075/150‑1 (MQ=255)

                                                                                                   tGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCa                                                    <  2:7051359/150‑1 (MQ=255)

                                                                                                      aGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATcc                                                 <  2:7300179/150‑1 (MQ=255)

                                                                                                        gTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAg                                               >  2:4466223/1‑150 (MQ=255)

                                                                                                        gTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAAAACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAg                                               >  1:773759/1‑150 (MQ=255)

                                                                                                         tGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAgg                                              <  2:1978362/150‑1 (MQ=255)

                                                                                                              ggCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGaa                                         <  2:2797602/150‑1 (MQ=255)

                                                                                                              ggCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGaa                                         <  1:3100672/150‑1 (MQ=255)

                                                                                                              ggCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGaa                                         <  1:1519536/150‑1 (MQ=255)

                                                                                                               gCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAg                                        <  1:161647/150‑1 (MQ=255)

                                                                                                                ccAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGt                                       >  1:4552316/1‑150 (MQ=255)

                                                                                                                 caacTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTc                                      >  1:5087013/4‑150 (MQ=255)

                                                                                                                    cTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGcc                                   <  1:4195359/150‑1 (MQ=255)

                                                                                                                    cTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGcc                                   <  1:4448980/150‑1 (MQ=255)

                                                                                                                     tCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGATGTCATCCAGGCCGAAGTCGccc                                  >  2:4783207/1‑150 (MQ=255)

                                                                                                                       ggTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCccag                                >  1:6388566/1‑150 (MQ=255)

                                                                                                                       ggTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCccag                                <  2:2175220/150‑1 (MQ=255)

                                                                                                                         tATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCccagca                              <  2:1189154/150‑1 (MQ=255)

                                                                                                                          aTCCGGGGCGGTACCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCccagcac                             <  2:3170582/150‑1 (MQ=255)

                                                                                                                           tCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGtt                                                                                                           <  1:4340717/71‑1 (MQ=255)

                                                                                                                           tCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCccagcacc                            >  1:4079894/1‑150 (MQ=255)

                                                                                                                               gggCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGa                                                                                 >  1:3880505/1‑93 (MQ=255)

                                                                                                                                  ccaatgtgAGCCTGGACGTGACGGTACCGTCCAGGCTCGCATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCt                     >  2:5873841/8‑150 (MQ=21)

                                                                                                                                  cGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAatgtat                                                                                                   <  1:114333/72‑1 (MQ=255)

                                                                                                                                   ggTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCtt                    <  2:6969366/150‑1 (MQ=255)

                                                                                                                                      gCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTtgtg                 <  2:4825793/150‑1 (MQ=255)

                                                                                                                                         gAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCgg              >  1:3476516/1‑150 (MQ=255)

                                                                                                                                            ccTGGACGTGACGGTACCGTCCAGGCTCGCATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAgc                                                                <  1:2671653/97‑1 (MQ=21)

                                                                                                                                              tGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGCt         >  2:6179691/1‑150 (MQ=255)

                                                                                                                                              tGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGCt         >  2:3498123/1‑150 (MQ=255)

                                                                                                                                                gACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGCTcc       >  1:4629645/1‑150 (MQ=255)

                                                                                                                                                  cGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGCTCCAc     <  1:5963455/150‑1 (MQ=255)

                                                                                                                                                   ggTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATACAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGCTCCAcc    >  2:2153018/1‑150 (MQ=255)

                                                                                                                                                     tACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGCTCCACCTc  >  1:5313272/1‑150 (MQ=255)

                                                                                                                                                     tACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGCTCCACCTc  >  1:6306854/1‑150 (MQ=255)

TTCAGGGCGACCAGTGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGCTCCACCTC  >  NC_002947/3005608‑3005906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`