BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,345,082	G→C	44.7%	intergenic (+74/‑257)	ribAB‑II → / → PP_3814	3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/polyamine ABC transportersubstrate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,345,082	0	G	C	44.7%	107.5 / 187.8	123	intergenic (+74/‑257)	ribAB‑II/PP_3814	3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/polyamine ABC transportersubstrate‑binding protein
Reads supporting (aligned to +/- strand): ref base G (40/28); new base C (31/24); total (71/52)
Fisher's exact test for biased strand distribution p-value = 8.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

Only 100 of 123 total aligned reads displayed.

TACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGCACCTA  >  NC_002947/4344937‑4345230

tACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGc                                                                                                                                                  <  1:3250782/150‑1 (MQ=255)

 aCGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCa                                                                                                                                                 <  2:3316070/150‑1 (MQ=255)

      gggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTcgc                                                                                                                                            <  1:468329/150‑1 (MQ=255)

      gggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTcgc                                                                                                                                            <  1:2246479/150‑1 (MQ=255)

       ggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTcgcg                                                                                                                                           <  1:6451324/150‑1 (MQ=25)

       ggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTcgcg                                                                                                                                           <  1:6451371/150‑1 (MQ=25)

       ggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTcgcg                                                                                                                                           <  1:2518030/150‑1 (MQ=25)

       ggCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTcgcg                                                                                                                                           <  2:6902183/150‑1 (MQ=255)

        gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATTGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg                                                                                                                                          <  2:6384410/150‑1 (MQ=255)

        gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg                                                                                                                                          <  1:5520605/150‑1 (MQ=25)

        gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg                                                                                                                                          <  1:1775442/150‑1 (MQ=25)

        gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg                                                                                                                                          <  1:2537730/150‑1 (MQ=25)

        gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg                                                                                                                                          <  1:5597828/150‑1 (MQ=25)

        gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg                                                                                                                                          <  2:2779971/150‑1 (MQ=25)

        gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg                                                                                                                                          <  2:2233571/150‑1 (MQ=25)

        gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg                                                                                                                                          <  1:7247507/150‑1 (MQ=25)

        gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg                                                                                                                                          <  1:6787567/150‑1 (MQ=25)

        gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg                                                                                                                                          <  2:3960038/150‑1 (MQ=25)

        gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg                                                                                                                                          <  2:4755456/150‑1 (MQ=25)

        gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg                                                                                                                                          <  2:5218923/150‑1 (MQ=255)

        gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg                                                                                                                                          <  1:5899049/150‑1 (MQ=255)

        gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg                                                                                                                                          <  1:5229218/150‑1 (MQ=255)

        gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg                                                                                                                                          <  1:6965759/150‑1 (MQ=255)

        gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg                                                                                                                                          <  2:3795479/150‑1 (MQ=255)

        gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg                                                                                                                                          <  2:6159718/150‑1 (MQ=255)

        gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg                                                                                                                                          <  2:5269477/150‑1 (MQ=255)

        gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATCGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg                                                                                                                                          <  2:773009/150‑1 (MQ=25)

        gCTGGCGGGCTATGAGCTGGAGGTGGGGCAGAGCATCCCGTTCGACCCGGGGATGGATGCCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg                                                                                                                                          <  1:812355/150‑1 (MQ=25)

          tGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGt                                                                                                                                        <  1:4652640/150‑1 (MQ=25)

           ggCGGGCTATGAGCTGGAGGTGGTGCAGCGCATCCCGTTCGACCCGGGGATGGAGGGCTGAGAACACACAAGCAATTGCTTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTa                                                                                                                                       <  1:4906561/150‑1 (MQ=255)

           ggCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACCAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTa                                                                                                                                       <  2:5522073/150‑1 (MQ=255)

           ggCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTa                                                                                                                                       <  1:3877666/150‑1 (MQ=255)

                  tATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTcgc                                                                                                                                            >  1:1770402/1‑138 (MQ=25)

                  tATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTcgc                                                                                                                                            <  2:1770402/138‑1 (MQ=25)

                  tATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTAACCCCGAAAGGGAGGGTGACACCACCGACGCCATCGCGGGGAAACCCGc                                                                                                                                >  2:4640096/1‑150 (MQ=255)

                   aTGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTCTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCt                                                                                                                               <  2:5449617/150‑1 (MQ=255)

                         tGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACACGCAATTGATTTAGCGGGGCCCTGTAGGTGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACa                                                                                                                         <  2:4891651/150‑1 (MQ=18)

                         tGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg                                                                                                                                          <  1:1111049/133‑1 (MQ=21)

                         tGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg                                                                                                                                          >  2:1111049/1‑133 (MQ=21)

                                     agagCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTcgc                                                                                                                                            >  2:2808544/1‑119 (MQ=255)

                                     agagCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTcgc                                                                                                                                            <  1:2808544/119‑1 (MQ=255)

                                         cATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAAc                                                                                                                                    <  1:3514825/123‑1 (MQ=255)

                                         cATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAAc                                                                                                                                    >  2:3514825/1‑123 (MQ=255)

                                                                                                             aGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGat                                     >  2:2390274/1‑150 (MQ=21)

                                                                                                                gggTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATc                                  >  1:16073/1‑150 (MQ=255)

                                                                                                                  gTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTg                                                      <  2:364002/128‑1 (MQ=255)

                                                                                                                  gTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATccc                                >  2:6413132/1‑150 (MQ=255)

                                                                                                                  gTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATccc                                >  2:1353461/1‑150 (MQ=255)

                                                                                                                  gTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCAGACCTCTTGCCAAAATCTTGGAATACCATAATATGATATccc                                >  2:5090675/1‑150 (MQ=255)

                                                                                                                       cccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCtt                                                                  >  2:7056897/1‑111 (MQ=18)

                                                                                                                       cccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGa                           >  1:5925683/1‑150 (MQ=255)

                                                                                                                        ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACCGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCCTACCTCTTGCCCAAATCTTGGAATACCACAATATGAGATCCCGTAGAc                          >  2:5273814/1‑150 (MQ=21)

                                                                                                                        ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCGAATATCTTGGAATAGCATAATATGTTATCGAGTAGag                          >  2:7331280/1‑149 (MQ=21)

                                                                                                                        ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:4239642/1‑150 (MQ=25)

                                                                                                                        ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:1283761/1‑150 (MQ=25)

                                                                                                                        ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:287746/1‑150 (MQ=25)

                                                                                                                        ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  2:5226009/1‑150 (MQ=25)

                                                                                                                        ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:106259/1‑150 (MQ=25)

                                                                                                                        ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  2:3731877/1‑150 (MQ=25)

                                                                                                                        ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:3732026/1‑150 (MQ=25)

                                                                                                                        ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:687307/1‑150 (MQ=25)

                                                                                                                        ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:5317131/1‑150 (MQ=25)

                                                                                                                        ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  2:7098403/1‑150 (MQ=25)

                                                                                                                        ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGAGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTAGCCAAAATCTTGGAATACCATAATCTGATAACCCGTAGAc                          >  1:4989435/1‑150 (MQ=21)

                                                                                                                        ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCCTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:4028012/1‑150 (MQ=25)

                                                                                                                        ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCCTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:2609938/1‑150 (MQ=25)

                                                                                                                        ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  2:5389318/1‑150 (MQ=255)

                                                                                                                        ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:4449835/1‑150 (MQ=255)

                                                                                                                        ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  2:1624461/1‑150 (MQ=255)

                                                                                                                        ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:7060068/1‑150 (MQ=255)

                                                                                                                        ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:534271/1‑150 (MQ=255)

                                                                                                                        ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCGTGCCAAAATCTTGGAATACCATAATATGATCTCCCGTAGAc                          >  1:6451748/1‑150 (MQ=255)

                                                                                                                        ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCCCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc                          >  1:2992002/1‑150 (MQ=255)

                                                                                                                         cgcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAcc                         >  1:753434/1‑150 (MQ=25)

                                                                                                                         cgcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAcc                         >  1:6805215/1‑150 (MQ=25)

                                                                                                                         cgcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTGGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATCCCATAATATGATATCCCGTAGAcc                         >  2:4460474/1‑150 (MQ=25)

                                                                                                                         cgcgAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAcc                         >  1:14915/1‑150 (MQ=255)

                                                                                                                         cgcgAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAcc                         >  1:3791236/1‑150 (MQ=255)

                                                                                                                          ggtgaTGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCt                        <  2:5718526/146‑1 (MQ=255)

                                                                                                                          gcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCtt                                                                  >  1:3091179/1‑108 (MQ=18)

                                                                                                                          gcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCtt                                                                  <  2:3091179/108‑1 (MQ=18)

                                                                                                                          gcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCtt                                                                  <  2:3090531/108‑1 (MQ=18)

                                                                                                                          gcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCt                        >  2:4547522/1‑150 (MQ=25)

                                                                                                                          gcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCt                        >  2:3791491/1‑150 (MQ=25)

                                                                                                                          gcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCt                        >  1:6067092/1‑150 (MQ=25)

                                                                                                                          gcgAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCt                        >  2:737542/1‑150 (MQ=255)

                                                                                                                          gcgAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCt                        >  2:6443436/1‑150 (MQ=255)

                                                                                                                          gcgAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGACCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCt                        >  1:6043975/1‑150 (MQ=255)

                                                                                                                               tGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGt                   >  2:2088954/1‑150 (MQ=255)

                                                                                                                                  gAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATa                                                 >  1:2739558/1‑117 (MQ=255)

                                                                                                                                  gAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATa                                                 <  2:2740329/117‑1 (MQ=255)

                                                                                                                                  gAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTg                >  2:6896550/1‑150 (MQ=255)

                                                                                                                                        gACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATCTCCCGTAGACCTTGAGTCTGCAGGcc          >  1:3284992/1‑150 (MQ=255)

                                                                                                                                          tgtcaccGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCgg        >  2:2665299/4‑150 (MQ=255)

                                                                                                                                           accaccGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGc       >  2:759685/1‑150 (MQ=255)

                                                                                                                                           accaccGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGc       >  1:1054277/1‑150 (MQ=255)

                                                                                                                                             caccGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGCAc     >  1:1609546/1‑150 (MQ=255)

                                                                                                                                              accGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGCAcc    >  2:4133971/1‑150 (MQ=255)

                                                                                                                                              accGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGCAcc    >  1:7121514/1‑150 (MQ=255)

                                                                                                                                                cGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGCAccaa  >  1:2795871/1‑148 (MQ=255)

TACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACCTTGAGTCTGCAGGCCGGCACCTA  >  NC_002947/4344937‑4345230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`