BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,450,243	C→G	6.2%	G117A (GGG→GCG) ‡	nicE ←	maleate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,450,243	0	C	G	6.2%	1261.0 / 52.9	371	G117A (GGG→GCG) ‡	nicE	maleate isomerase
Reads supporting (aligned to +/- strand): ref base C (191/157); new base G (9/14); total (200/171)
Fisher's exact test for biased strand distribution p-value = 1.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 384 total aligned reads displayed.

TCGGGGATTTCCAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCGGACAGC  >  NC_002947/4450096‑4450392

  ggggATTTCCAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGgc                                                                                                                                                   >  1:3244143/1‑150 (MQ=255)

   gggATTTCCAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGgcg                                                                                                                                                  >  2:6219725/1‑150 (MQ=255)

      aTTTCCAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTg                                                                                                                                               <  2:1934100/150‑1 (MQ=255)

       tttCCAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTgg                                                                                                                                              <  2:6062583/150‑1 (MQ=255)

       tttCCAGCGCGCGCCAGTCCTGCACCTCGAAGCCGTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCGGGGCGCTgg                                                                                                                                              <  2:1891408/150‑1 (MQ=255)

         tCCAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCGGGGCGCTGGtc                                                                                                                                            <  2:5442409/150‑2 (MQ=255)

           cAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCGGGGCGCTGGtcga                                                                                                                                          <  1:1565216/150‑4 (MQ=255)

           cAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGAt                                                                                                                                          >  1:6305622/1‑150 (MQ=255)

            aGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATc                                                                                                                                         <  2:4072840/150‑1 (MQ=255)

                  cgcCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGt                                                                                                                                   <  2:4572718/150‑1 (MQ=255)

                   gcCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTg                                                                                                                                  >  1:3037733/1‑150 (MQ=255)

                   gcCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTg                                                                                                                                  >  2:2882212/1‑150 (MQ=255)

                     cAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGcc                                                                                                                                >  2:3908735/1‑150 (MQ=255)

                      aGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCa                                                                                                                               <  1:71175/150‑1 (MQ=255)

                        tCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGt                                                                                                                             <  2:5210334/150‑1 (MQ=255)

                         ccTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTg                                                                                                                            >  2:68023/1‑150 (MQ=255)

                          cTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGc                                                                                                                           <  1:5780852/150‑1 (MQ=255)

                          cTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGc                                                                                                                           >  1:43335/1‑150 (MQ=255)

                            gCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACTGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGcat                                                                                                                         >  2:3349332/1‑150 (MQ=255)

                              aCCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGcatca                                                                                                                       >  2:2806587/1‑150 (MQ=255)

                                cTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCAtt                                                                                                                     <  1:4207631/150‑1 (MQ=255)

                                 tCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTg                                                                                                                    >  2:6006035/1‑150 (MQ=255)

                                 tCGAAGCCTTCCTCGCGGATGTAGTCCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACAATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCAtt                                                                                                                     >  2:2403455/1‑149 (MQ=255)

                                 tCGAAGCCTTCCTCGCGGATGTAGTCCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACAATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCAtt                                                                                                                     <  1:2403455/149‑1 (MQ=255)

                                    aaGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTcg                                                                                                                 >  2:7035047/1‑150 (MQ=255)

                                         ttCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGc                                                                                                                           >  1:5594191/1‑135 (MQ=255)

                                         ttCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGt                                                                                                            >  2:579258/1‑150 (MQ=255)

                                         ttCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGt                                                                                                            >  2:5618379/1‑150 (MQ=255)

                                          tCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTg                                                                                                           <  1:3816906/150‑1 (MQ=255)

                                            cTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGc                                                                                                         >  2:148828/1‑150 (MQ=255)

                                             tCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGcc                                                                                                        >  1:6398899/1‑150 (MQ=255)

                                             tCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGcc                                                                                                        >  2:85658/1‑150 (MQ=255)

                                              cgcgGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCct                                                                                                       <  1:5400/150‑1 (MQ=255)

                                              cgcgGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCct                                                                                                       >  1:6150615/1‑150 (MQ=255)

                                                 ggATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCctgct                                                                                                    <  1:3784608/150‑1 (MQ=255)

                                                      tAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAgg                                                                                               >  2:1151261/1‑150 (MQ=255)

                                                         ttCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGgcgc                                                                                            <  1:259353/150‑1 (MQ=255)

                                                         ttCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGgcgc                                                                                            <  2:1175455/150‑1 (MQ=255)

                                                            accaccAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCttt                                                                                         >  1:4567194/1‑150 (MQ=255)

                                                              caccaACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTc                                                                                       >  2:4165534/1‑150 (MQ=255)

                                                              caccaACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTc                                                                                       >  2:6424169/1‑150 (MQ=255)

                                                              caccaACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTc                                                                                       >  1:906453/1‑150 (MQ=255)

                                                               accaACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCa                                                                                      <  1:2469432/150‑1 (MQ=255)

                                                               accaACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCa                                                                                      >  1:6570032/1‑150 (MQ=255)

                                                                  aaCTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGAt                                                                                   <  1:7058876/150‑1 (MQ=255)

                                                                      cGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGc                                                                               <  1:6604532/150‑1 (MQ=255)

                                                                       ggTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCg                                                                              <  2:6305622/150‑1 (MQ=255)

                                                                       ggTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCg                                                                              <  2:1828949/150‑1 (MQ=255)

                                                                        gTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCgg                                                                             >  2:2108166/1‑150 (MQ=255)

                                                                         tCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGt                                                                            <  1:5740228/150‑1 (MQ=255)

                                                                         tCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGt                                                                            <  2:674512/150‑1 (MQ=255)

                                                                         tCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGt                                                                            >  2:2621960/1‑150 (MQ=255)

                                                                         tCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGt                                                                            <  1:5148783/150‑1 (MQ=255)

                                                                          cAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTg                                                                           <  1:3908735/150‑1 (MQ=255)

                                                                           aGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGa                                                                          <  1:4889603/150‑1 (MQ=255)

                                                                             cGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATa                                                                        >  1:104026/1‑150 (MQ=255)

                                                                                   cATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATagcccag                                                                  >  2:5547780/1‑150 (MQ=255)

                                                                                    aTGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATagctcagc                                                                 <  2:1336859/150‑1 (MQ=255)

                                                                                         cGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCAt                                                            <  1:5492716/150‑1 (MQ=255)

                                                                                           gcgcGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATgg                                                          <  1:980907/150‑1 (MQ=255)

                                                                                             gcgACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGcc                                                        >  2:1657851/1‑150 (MQ=255)

                                                                                              cgacgaTGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCa                                                       <  2:5160568/150‑1 (MQ=255)

                                                                                                   aTGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATc                                                  <  2:3216621/150‑1 (MQ=255)

                                                                                                             gcTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAaggc                                        >  2:273264/1‑150 (MQ=255)

                                                                                                              cTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAaggca                                       <  1:7170199/150‑1 (MQ=255)

                                                                                                              cTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAaggca                                       <  1:498619/150‑1 (MQ=255)

                                                                                                              cTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAaggca                                       <  1:853696/150‑1 (MQ=255)

                                                                                                               ttGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAaggcag                                      >  2:4838927/1‑150 (MQ=255)

                                                                                                               ttGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAaggcag                                      >  2:5114908/1‑150 (MQ=255)

                                                                                                               ttGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAaggcag                                      <  1:6883463/150‑1 (MQ=255)

                                                                                                                  gCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAggcaggca                                   >  2:3803493/1‑150 (MQ=255)

                                                                                                                   ccTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCAt                                  >  2:4296564/1‑150 (MQ=255)

                                                                                                                    cTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATa                                 <  1:2963213/150‑1 (MQ=255)

                                                                                                                    cTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATa                                 >  1:1678389/1‑150 (MQ=255)

                                                                                                                         tcaCATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCa                            <  2:654900/150‑1 (MQ=255)

                                                                                                                          cacaTGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCg                                                                              >  1:1784236/1‑99 (MQ=255)

                                                                                                                           acaTGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGc                          >  2:2278600/1‑150 (MQ=255)

                                                                                                                             aTGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGcac                        >  1:1514963/1‑150 (MQ=255)

                                                                                                                                 aaGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCg                    >  2:3563884/1‑150 (MQ=255)

                                                                                                                                 aaGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCg                    >  1:761958/1‑150 (MQ=255)

                                                                                                                                    gCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGAcc                 >  2:6146772/1‑150 (MQ=255)

                                                                                                                                       tCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGTCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTg              >  2:3064237/1‑150 (MQ=255)

                                                                                                                                       tCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTg              <  1:649081/150‑1 (MQ=255)

                                                                                                                                        tcaCCAGCGCCGGGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTgg             >  1:7317248/3‑150 (MQ=255)

                                                                                                                                        tcaCCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGcc                                                        <  2:3668715/105‑1 (MQ=255)

                                                                                                                                        tcaCCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGcc                                                        >  1:3668715/3‑107 (MQ=255)

                                                                                                                                        cGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTgg             <  1:1237445/150‑1 (MQ=255)

                                                                                                                                         caCCAGCGCCGGGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGa                                                                                    <  2:7144346/77‑1 (MQ=255)

                                                                                                                                         caCCAGCGCCGGGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTGTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGc            >  2:4490254/2‑150 (MQ=255)

                                                                                                                                          aCCAGCGCCGGGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCg           >  1:6777730/1‑150 (MQ=255)

                                                                                                                                          aCCAGCGCCGGGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCg           >  1:6035765/1‑150 (MQ=255)

                                                                                                                                            cAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTc         >  1:2036134/1‑150 (MQ=255)

                                                                                                                                            cAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTc         <  1:517688/150‑1 (MQ=255)

                                                                                                                                            cAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTc         >  1:2887401/1‑150 (MQ=255)

                                                                                                                                             aGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATgg                                                          <  1:3572842/100‑1 (MQ=255)

                                                                                                                                             aGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCg        <  1:3355807/150‑1 (MQ=255)

                                                                                                                                             aGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCg        <  2:5064999/150‑1 (MQ=255)

                                                                                                                                                 ccccGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCGGACa    >  1:2069590/1‑150 (MQ=255)

                                                                                                                                                  cccGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCGGACAg   <  2:5030680/150‑1 (MQ=255)

                                                                                                                                                  cccGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCGGACAg   >  2:6616415/1‑150 (MQ=255)

TCGGGGATTTCCAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCGGACAGC  >  NC_002947/4450096‑4450392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`