BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,450,244	C→G	6.2%	G117R (GGG→CGG) ‡	nicE ←	maleate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,450,244	0	C	G	6.2%	1269.7 / 54.0	371	G117R (GGG→CGG) ‡	nicE	maleate isomerase
Reads supporting (aligned to +/- strand): ref base C (191/157); new base G (9/14); total (200/171)
Fisher's exact test for biased strand distribution p-value = 1.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 385 total aligned reads displayed.

TCGGGGATTTCCAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCGGACAGCT  >  NC_002947/4450096‑4450393

tCGGGGATTTCCAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCg                                                                                                                                                      <  1:3825320/150‑1 (MQ=255)

  ggggATTTCCAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGgc                                                                                                                                                    >  1:3244143/1‑150 (MQ=255)

   gggATTTCCAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGgcg                                                                                                                                                   >  2:6219725/1‑150 (MQ=255)

      aTTTCCAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTg                                                                                                                                                <  2:2011807/150‑1 (MQ=255)

       tttCCAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTgg                                                                                                                                               <  2:6062583/150‑1 (MQ=255)

       tttCCAGCGCGCGCCAGTCCTGCACCTCGAAGCCGTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCGGGGCGCTgg                                                                                                                                               <  2:1891408/150‑1 (MQ=255)

         tCCAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCGGGGCGCTGGtc                                                                                                                                             <  2:5442409/150‑2 (MQ=255)

           cAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCGGGGCGCTGGtcga                                                                                                                                           <  1:1565216/150‑4 (MQ=255)

           cAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGAt                                                                                                                                           >  1:6305622/1‑150 (MQ=255)

            aGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATc                                                                                                                                          <  2:4072840/150‑1 (MQ=255)

                  cgcCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGt                                                                                                                                    <  2:4572718/150‑1 (MQ=255)

                     cAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGcc                                                                                                                                 >  2:3908735/1‑150 (MQ=255)

                     cAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGcc                                                                                                                                 >  1:3049619/1‑150 (MQ=255)

                      aGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCa                                                                                                                                <  1:71175/150‑1 (MQ=255)

                        tCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGt                                                                                                                              <  2:5210334/150‑1 (MQ=255)

                         ccTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTg                                                                                                                             >  2:68023/1‑150 (MQ=255)

                          cTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGc                                                                                                                            >  1:43335/1‑150 (MQ=255)

                          cTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGc                                                                                                                            <  2:1495145/150‑1 (MQ=255)

                           tGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCGGGGCGCTGGtcga                                                                                                                                           >  2:5875335/1‑131 (MQ=255)

                           tGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGca                                                                                                                           <  2:1223709/150‑1 (MQ=255)

                                cTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCAtt                                                                                                                      <  1:4207631/150‑1 (MQ=255)

                                 tCGAAGCCTTCCTCGCGGATGTAGTCCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACAATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCAtt                                                                                                                      <  1:2403455/149‑1 (MQ=255)

                                   gAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTc                                                                                                                   >  1:767009/1‑150 (MQ=255)

                                    aaGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTcg                                                                                                                  >  2:7035047/1‑150 (MQ=255)

                                        cTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTcggcgg                                                                                                              >  1:5792387/1‑150 (MQ=255)

                                         ttCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGc                                                                                                                            >  1:5594191/1‑135 (MQ=255)

                                         ttCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGt                                                                                                             >  2:5618379/1‑150 (MQ=255)

                                         ttCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGt                                                                                                             >  2:1374735/1‑150 (MQ=255)

                                         ttCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGt                                                                                                             >  2:579258/1‑150 (MQ=255)

                                          tCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTg                                                                                                            <  2:3076094/150‑1 (MQ=255)

                                            cTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGc                                                                                                          >  1:6046529/1‑150 (MQ=255)

                                             tCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGcc                                                                                                         >  2:85658/1‑150 (MQ=255)

                                             tCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGcc                                                                                                         >  1:6398899/1‑150 (MQ=255)

                                              cgcgGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCct                                                                                                        <  1:5400/150‑1 (MQ=255)

                                              cgcgGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCct                                                                                                        >  1:6150615/1‑150 (MQ=255)

                                                 ggATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCctgct                                                                                                     <  1:3784608/150‑1 (MQ=255)

                                                     gTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAg                                                                                                 <  2:2404243/150‑1 (MQ=255)

                                                      tAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAgg                                                                                                >  2:1151261/1‑150 (MQ=255)

                                                         ttCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGgcgc                                                                                             <  1:259353/150‑1 (MQ=255)

                                                            accaccAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCttt                                                                                          >  1:4567194/1‑150 (MQ=255)

                                                              caccaACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCACTGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTc                                                                                        >  2:3312030/1‑150 (MQ=255)

                                                              caccaACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTc                                                                                        >  2:4165534/1‑150 (MQ=255)

                                                              caccaACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTc                                                                                        >  1:7189068/1‑150 (MQ=255)

                                                              caccaACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTc                                                                                        >  1:906453/1‑150 (MQ=255)

                                                              caccaACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTc                                                                                        >  2:6424169/1‑150 (MQ=255)

                                                               accaACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCa                                                                                       <  1:2469432/150‑1 (MQ=255)

                                                                  aaCTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGAt                                                                                    <  1:7058876/150‑1 (MQ=255)

                                                                       ggTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCg                                                                               <  2:6305622/150‑1 (MQ=255)

                                                                       ggTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCg                                                                               <  2:1828949/150‑1 (MQ=255)

                                                                         tCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGt                                                                             <  1:5740228/150‑1 (MQ=255)

                                                                         tCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGt                                                                             >  2:2621960/1‑150 (MQ=255)

                                                                         tCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGt                                                                             <  2:674512/150‑1 (MQ=255)

                                                                           aGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGa                                                                           <  1:4889603/150‑1 (MQ=255)

                                                                             cGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATa                                                                         >  1:104026/1‑150 (MQ=255)

                                                                                  tCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATagccca                                                                    <  2:2284377/150‑1 (MQ=255)

                                                                                   cATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATagcccag                                                                   >  2:5547780/1‑150 (MQ=255)

                                                                                    aTGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATagcccagc                                                                  >  1:739459/1‑150 (MQ=255)

                                                                                         cGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCAt                                                             <  1:5492716/150‑1 (MQ=255)

                                                                                          ggCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATg                                                            >  1:6653036/1‑150 (MQ=255)

                                                                                           gcgcGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATgg                                                           <  1:980907/150‑1 (MQ=255)

                                                                                             gcgACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGcc                                                         >  2:1657851/1‑150 (MQ=255)

                                                                                                    tGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCg                                                  >  2:3502956/1‑150 (MQ=255)

                                                                                                             gcTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAaggc                                         >  2:273264/1‑150 (MQ=255)

                                                                                                              cTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAaggca                                        <  1:498619/150‑1 (MQ=255)

                                                                                                              cTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAaggca                                        <  1:853696/150‑1 (MQ=255)

                                                                                                               tttgCCTTCATCACATGCACGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAaggcag                                       <  2:2153070/147‑1 (MQ=255)

                                                                                                               ttGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAaggcag                                       >  2:5114908/1‑150 (MQ=255)

                                                                                                               ttGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAaggcag                                       >  2:4838927/1‑150 (MQ=255)

                                                                                                               ttGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAaggcag                                       <  1:6883463/150‑1 (MQ=255)

                                                                                                                  gCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAggcaggca                                    >  2:3803493/1‑150 (MQ=255)

                                                                                                                    cTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATa                                  >  1:1678389/1‑150 (MQ=255)

                                                                                                                    cTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATa                                  <  1:2963213/150‑1 (MQ=255)

                                                                                                                      tcatcaCATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGc                                <  2:4378908/150‑1 (MQ=255)

                                                                                                                         tcaCATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCa                             <  2:654900/150‑1 (MQ=255)

                                                                                                                         tcaCATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCa                             >  2:2818247/1‑150 (MQ=255)

                                                                                                                          cacaTGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCg                                                                               >  1:1784236/1‑99 (MQ=255)

                                                                                                                           acaTGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGc                           >  2:4211108/1‑150 (MQ=255)

                                                                                                                             aTGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGcac                         >  1:1514963/1‑150 (MQ=255)

                                                                                                                                    gCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGAcc                  >  2:6146772/1‑150 (MQ=255)

                                                                                                                                       tCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTg               <  1:6586296/150‑1 (MQ=255)

                                                                                                                                       tCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTg               <  1:649081/150‑1 (MQ=255)

                                                                                                                                        tcaCCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGcc                                                         <  2:3668715/105‑1 (MQ=255)

                                                                                                                                        tcaCCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGcc                                                         >  1:3668715/3‑107 (MQ=255)

                                                                                                                                        cGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTgg              <  1:1237445/150‑1 (MQ=255)

                                                                                                                                         caCCAGCGCCGGGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGa                                                                                     <  2:7144346/77‑1 (MQ=255)

                                                                                                                                          aCCAGCGCCGGGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCg            >  1:6777780/1‑150 (MQ=255)

                                                                                                                                            cAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTc          >  1:2887401/1‑150 (MQ=255)

                                                                                                                                            cAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTc          <  1:517688/150‑1 (MQ=255)

                                                                                                                                            cAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTc          >  1:2036134/1‑150 (MQ=255)

                                                                                                                                             aGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATgg                                                           <  1:3572842/100‑1 (MQ=255)

                                                                                                                                             aGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCg         >  2:2953915/1‑150 (MQ=255)

                                                                                                                                             aGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCg         <  1:3355807/150‑1 (MQ=255)

                                                                                                                                             aGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCg         <  2:5064999/150‑1 (MQ=255)

                                                                                                                                                 ccccGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCGGACa     >  1:2069590/1‑150 (MQ=255)

                                                                                                                                                  cccGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCGGACa     >  1:5152385/1‑149 (MQ=255)

                                                                                                                                                  cccGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCGGACa     <  2:5152385/149‑1 (MQ=255)

                                                                                                                                                  cccGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCGGACAg    >  2:6616415/1‑150 (MQ=255)

                                                                                                                                                  cccGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCGGACAg    <  2:5030680/150‑1 (MQ=255)

                                                                                                                                                   ccGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCGGACAGc   <  1:3984158/150‑1 (MQ=255)

                                                                                                                                                    cGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCGGACAGCt  <  2:3244143/150‑1 (MQ=255)

TCGGGGATTTCCAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCGGACAGCT  >  NC_002947/4450096‑4450393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`