Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,822,6300CG25.0% 79.2 / 29.8 44F207L (TTC→TTG) bamA‑IIouter membrane protein assembly factor
Reads supporting (aligned to +/- strand):  ref base C (10/23);  new base G (0/11);  total (10/34)
Fisher's exact test for biased strand distribution p-value = 4.57e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GCGCAACCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAACGACGACAAGT  >  NC_002947/3822483‑3822646
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gcgcAACCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCa                 <  1:8701003/149‑1 (MQ=32)
gcgcAACCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCa                 <  1:7510249/149‑1 (MQ=32)
gcgcAACCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCa                 <  1:7510219/149‑1 (MQ=32)
gcgcAACCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCa                 >  2:8162432/1‑149 (MQ=32)
gcgcAACCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCa                 <  1:6216919/149‑1 (MQ=32)
   cAACCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACACCGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGa              <  1:711476/149‑1 (MQ=17)
   cAACCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGa              <  1:6750401/149‑1 (MQ=32)
   cAACCGTGTCGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGa              <  2:3271639/149‑1 (MQ=17)
    aaCCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGaa             >  1:1543252/1‑149 (MQ=32)
     aCCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAac            >  1:436006/1‑149 (MQ=32)
     aCCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAac            >  1:435618/1‑149 (MQ=32)
     aCCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAac            >  1:1122545/1‑149 (MQ=32)
      ccGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTGAAGAacg           <  2:4345532/149‑1 (MQ=17)
      ccGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAacg           <  1:2401659/149‑1 (MQ=32)
      ccGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAacg           <  2:4309511/149‑1 (MQ=32)
      ccGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAacg           <  1:80456/149‑1 (MQ=32)
      ccGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAacg           <  1:1906696/149‑1 (MQ=32)
       cGTGTTGCGCAGAAGATCAAGATCAACGAAGGCACCGTCGCAGCAATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCATGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGATTTCGATATTCAATAaaga          >  2:7683730/1‑149 (MQ=11)
       cGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTGAAGAacga          <  2:5125139/149‑1 (MQ=17)
       cGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAacga          >  2:5246715/1‑149 (MQ=32)
       cGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAacga          <  2:8522820/149‑1 (MQ=32)
       cGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAacga          <  2:8589945/149‑1 (MQ=32)
        gtgtGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTGAAGAacgac         <  2:2441854/149‑1 (MQ=17)
        gtgtGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTGAAGAacgac         <  2:2819192/149‑1 (MQ=17)
        gtgtGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTGAAGAacgac         <  2:2819317/149‑1 (MQ=17)
        gtgtGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAacgac         <  2:3329044/149‑1 (MQ=32)
        gtgtGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAacgac         <  2:3329109/149‑1 (MQ=32)
         tgtgGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTGAAGAacgaca        <  1:7644187/149‑2 (MQ=17)
         tgtgGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTGAAGAacgaca        <  1:7644997/149‑2 (MQ=17)
         tgtgGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTGAAGAacgaca        <  2:2280164/149‑2 (MQ=17)
         tgtgGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTGAAGAacgaca        <  2:4195988/149‑2 (MQ=17)
         tgtgGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAacgacg        >  1:7806353/1‑149 (MQ=32)
         tgtgGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAacgacg        <  1:6328818/149‑1 (MQ=32)
          gtgGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTGAAGAacgacag       <  1:3672243/149‑3 (MQ=17)
          gtgGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAAcgacga       <  2:7621326/149‑1 (MQ=32)
          gtgGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAAcgacga       <  1:4579741/149‑1 (MQ=32)
             gcgcTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAACGACGACaa    <  2:1051147/149‑1 (MQ=32)
             gcgcTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAACGACGACaa    >  2:542589/1‑149 (MQ=32)
             gcgcTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAACGACGACaa    >  2:5458817/1‑149 (MQ=32)
              cgcTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTGAAGAacgacagccag   <  1:4122662/149‑7 (MQ=9)
              cgcTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAACGACGACAAg   <  2:3926041/149‑1 (MQ=31)
               gcTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAACGACGACAAGt  <  2:3042667/149‑1 (MQ=11)
               gcTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAACGACGACAAGt  <  2:2629001/149‑1 (MQ=11)
               gcTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAACGACGACAAGt  <  1:3246349/149‑1 (MQ=11)
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GCGCAACCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAACGACGACAAGT  >  NC_002947/3822483‑3822646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑