Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,729,3060TG22.7% 532.1 / 23.5 266intergenic (‑203/+91)PP_5024/opgHamino acid ABC transporter substrate‑binding protein/glucans biosynthesis glucosyltransferase H
Reads supporting (aligned to +/- strand):  ref base T (79/119);  new base G (41/17);  total (130/136)
Fisher's exact test for biased strand distribution p-value = 4.42e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 275 total aligned reads displayed.

ACTGGCCGAAGCGTCGTATCGGAGAGGGATGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCAGGGTGCAACGGCAACAGCGGC  >  NC_002947/5729158‑5729454
                                                                                                                                                      |                                                                                                                                                     
 cTGGCCGAAGCGTCGTATCGGAGAGGGATGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCATGTGTATCCCTg                                                                                                                                                      <  2:1856611/149‑1 (MQ=255)
 cTGGCCGAAGCGTCGTATCGGAGAGGGATGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCATGTGTATCCCTg                                                                                                                                                      <  1:3376066/149‑1 (MQ=255)
 cTGGCCGAAGCGTCGTATCGGAGAGGGATGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCATGTGTATCCCTg                                                                                                                                                      <  2:7667748/149‑1 (MQ=255)
 cTGGCCGAAGCGTCGTATCGGAGAGGGATGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCAACAAGGGCATGTGTATCCCTg                                                                                                                                                      >  2:3560179/1‑149 (MQ=255)
 cTGGCCGAAGCGGCGTATCGGCGAGGGATTCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCTACCAGGGCAAGTGTATCCCTg                                                                                                                                                      <  2:452051/149‑1 (MQ=255)
  tGGCCGAAGCGTCGTATCGGAGAGGGATGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAAACCGCTCCTACAAGGGCATGTTTATACCggg                                                                                                                                                     >  1:5066219/1‑149 (MQ=255)
   ggCCGAAGCGTCGTATCGGAGAGGGATGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCATGTGTATCCCTGGt                                                                                                                                                    <  2:8614876/149‑1 (MQ=255)
     ccGAAGCGTCGTATCGGAGAGGGATGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGTAACCCCGCCAACAAGGGGATGTGTATACCGGGTAg                                                                                                                                                  >  2:1784356/1‑149 (MQ=255)
      cGAAGCGTCGTATCGGAGAGGGATGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCATGTGTATCCCTGGTAgg                                                                                                                                                 <  1:3895262/149‑1 (MQ=255)
       gAAGCGTCGTATCGGAGAGGGATGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCAACAAGGGCATGTGTATACCTGGTAGGa                                                                                                                                                >  2:1704130/1‑149 (MQ=255)
       gAAGCGTCGTATCGGAGAGGGATGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCAACAAGGGCATGTGTATACCAGGTAGGa                                                                                                                                                >  2:1368590/1‑149 (MQ=255)
        aaGCGTCGTATCGGAGAGGGATGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGCCCAGCCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCATGTGTATCCCTGGTAGGAg                                                                                                                                               <  1:120404/149‑1 (MQ=255)
          gCGTCGTATCGGAGAGGGATGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGCCAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCATGTGTATCCCTGGTAGGAGCg                                                                                                                                             <  1:6103074/149‑1 (MQ=255)
            gtcgtATCGGAGAGGGATGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCATGTGTATCCCAGGTAGGAGCggg                                                                                                                                           >  2:2165641/1‑149 (MQ=255)
             tcgtATCGGAGAGGGATGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAACTGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCATGTGTATCCCTGGTAGGAGCGGGt                                                                                                                                          <  1:8305736/149‑1 (MQ=255)
              cgtATCGGAGAGGGATGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGTACCCCCGCCAAAAAGGGCACGTGGATGCCGGGGAGGGGCggggt                                                                                                                                         >  1:2419157/1‑147 (MQ=255)
              cgtATCGGAGAGGGATGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCATGTGTATCCCTGGTAGGAGCGGGtt                                                                                                                                         <  2:4709972/149‑1 (MQ=255)
              cgtATCGGAGAGGGATGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCATGTGTATCCCTGGTAGGAGCGGGtt                                                                                                                                         <  2:2482322/149‑1 (MQ=255)
              cgtATCGGAGAGGGATGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCATGTGTATCCCTGGTAGGAGCGGGtt                                                                                                                                         <  1:4466976/149‑1 (MQ=255)
                  tCGGAGAGGGATGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGGAACCGCGCCGACAAGGGCATGTGTATCCCTGGGAGGCGCGGGttgaac                                                                                                                                     >  2:3249410/1‑145 (MQ=255)
                      agagGGATGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAAAACGCTCCTACAAGGGGGAGGGTATACCAGGTAGGAGAGGGGTGACCcgcc                                                                                                                                 >  1:5158882/1‑148 (MQ=255)
                      agagGGAAGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCTACAACGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCcgcg                                                                                                                                 >  2:5831211/1‑149 (MQ=255)
                          ggATGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGT‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCATGTGCATCCCTGGTAGGAGCGGGTTCACCCGCGAaga                                                                                                                             <  2:7340110/149‑1 (MQ=255)
                          ggATGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAaga                                                                                                                             <  2:8281617/149‑1 (MQ=255)
                             tGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGTACCCGCTCCTACAAGGGCATGTGTATCCCGGGTAGGAGCGGGTTCACCCGCGAAGAGGc                                                                                                                          >  2:661041/1‑149 (MQ=255)
                                tAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGGGAACCCCCGCCAAAAAGGGCATGTTTATACCAGGGTGGGGCGGGGTCACCCCCCCAGAGGcccc                                                                                                                       >  1:922569/1‑148 (MQ=255)
                                   cGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAT‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCATGTGTATACCGGGGAGGTGCGGGTTCACCCGCGCAGAGGCCCGGTc                                                                                                                    >  2:4642261/1‑149 (MQ=39)
                                         gcggcCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑Tgcgc                                                                                                             <  2:9035556/149‑1 (MQ=255)
                                            gcCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAAACCCCTCCAACAAGGGCAAGTGTATACCGGGTAGGAGCGGGTTCACCCCCGAAGAGGCCCGGTCA‑TGCGcccc                                                                                                          >  1:9020345/1‑149 (MQ=255)
                                                gtgtACCTCGAGCGGCGGGGGGACTTTGTTGGCCAACATGT‑GGGGGTTAGGCAAAAAT‑GCTTCGGGGTTTAACCCGCTCCTACCCGGGCTTGTGTTTCCCTGGTTGGAGGGGGTTAACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAg                                                                                                      <  2:3316712/149‑1 (MQ=255)
                                                gTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGACCCCGCTCCTACACGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAg                                                                                                      <  2:5893636/149‑1 (MQ=255)
                                                   gACATAGCGCCGCGGGGGGATTTTGTTGGCAAAATTGT‑GGGGGTTCGGCCAAAAC‑GCTGCGGGGGTGAACCCGCCCCTACACGGGCTTGTTTAGCCGTGGTTGGAGGGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGc                                                                                                   <  2:73056/149‑1 (MQ=255)
                                                     ccTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGGACCCCCGCCTACAAGGGCCTGTGTATCCCAGGGAGGAGCGGGGTCACCCCCGAAGAGGCCCGGGCA‑TGCGCCCCGGAGGGCgg                                                                                                 >  2:3795827/1‑149 (MQ=255)
                                                      cacgtgCCGCTGGGGGACTTTGTTGGCCAAACTGC‑GGGGGTCCGCCCAAACA‑GCTTCGCGGGTGACCCCGCGCCTACACGGGCATGTGTACCCCTGGTTGGTGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCggg                                                                                                <  1:7526934/144‑1 (MQ=255)
                                                       tAGAGCCGCAGGGGGACTTTGTTGGCCAAAGTGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGGTAAACCCCGCCAGCAAGGGCGGGGGGTTACCGGGGAGGGGCCGGGTGACCCCCGACGGGGGGCGGGGC‑TGCGCCCCGGAGGGCgggg                                                                                               >  1:5405881/1‑148 (MQ=255)
                                                         gagCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGTAACCCCGCCAACAAGGGCCAGTGTATACCAGGGAGGAGCGGGTTGCCCCCCCAACAGGCCCCGGCC‑GGCGCCCCCGAGGGCggggta                                                                                             >  1:4366638/1‑146 (MQ=255)
                                                            ccGCAGGGGGACTTTGTTGGCCAACATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCTCCAAGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATc                                                                                          <  2:7444010/149‑1 (MQ=255)
                                                             cGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCATGTGTATCCCTGGGAGGAGCGGGTTCACCCGCGCAGAGGCCCGGTCA‑TTCGCCCCGGAGGGCGGGTTCATCg                                                                                         >  2:4280979/1‑149 (MQ=255)
                                                              gcggggggACTTTGTT‑GCCAAATTGG‑TGGGGTTTCGCAAAAAATGCTGCGGGGGTCACCCCGCGCCTACACGGGCTTGTGTATCCCTGGTTGGCGGGGTTACACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCgg                                                                                        <  1:6271343/146‑1 (MQ=255)
                                                              gccgggggACTTTGTTGGCCAAACTGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCTACACGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCgg                                                                                        <  2:4424463/146‑1 (MQ=255)
                                                               cAGGGGGACTTTGTTGGCCACACTGC‑GGGGGTTCGGCCAAAAC‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCggg                                                                                       <  2:2809425/149‑1 (MQ=38)
                                                               cAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAT‑GCTTCGCGGGTGTAACCGGTGCTAGAAGGGGGTGTGTTTGGGTGGTTGGTGGGGGTTGTCCCGCGGAGTTGCCCGGTGATTGGGCCCGGTGGGGGGGGtgataggg                                                                                        >  2:8530087/1‑142 (MQ=255)
                                                               cAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAT‑GCTTCGCGGGTGTAACCGCTCCAACAAGGGGATGTGTATACCGGGTTGGTGGGGGTTGACCCGCGTAGTTGCCCCGGCA‑TGGGCCCCCTAGGGCGGGGTCATCggg                                                                                       >  1:3650467/1‑149 (MQ=255)
                                                                  ggggACTTGGTTGGCCAAAATGA‑GGGGGTTAGGCCAAACA‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCATGTGTATCCCTGGTAGGCGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGgcg                                                                                    <  2:4392682/149‑1 (MQ=255)
                                                                        tttGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAAACCGCTCCTAAAAGGGCAAGTGTATCCCGGGGAGGAGCGGGTTCAACCGCGAAGAGGCCCGGTCA‑TGCGCCCCCTAGGGCCGGTTCATCGGGGGGCCGAAc                                                                              >  2:3429145/1‑149 (MQ=255)
                                                                         ttgttgGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCGCCTACAAGGGCCTGTGTATCCCGGGGAGGCGCGGGGTCACCCGCGGAGAGGCCCGGTCC‑TGCGCCCCCTAGGGCGGGGTCAGCGGGGCGCCgcccc                                                                             >  2:4782950/1‑145 (MQ=255)
                                                                              gggcAAAATGGGGGGGGTGCGCCCAAAAC‑GCTTCGGGGGTCACCCCGCTCCTACACGGGCTTGTTTATCCGTGGTCGGCGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAAccgccg                                                                         <  1:7280027/146‑1 (MQ=255)
                                                                                ccAAAATGC‑GGGGGTTCGGCCAAAAA‑GCTTCGCGGGTTAACCCGCTCCTACAAGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGa                                                                      <  1:2096968/149‑1 (MQ=255)
                                                                                  aataTGA‑GGGGGTTAGGCCAAAAT‑GCTTCGCGGGTGAACCCGCTCCTACTAGGGCATGTGTATCCCTGGTTGGTGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAAc                                                                    <  2:3653603/146‑1 (MQ=39)
                                                                                  aaaaTGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGTAACCCCTCCAACAAGGGGCGGTGTATCACGGGTAGGATCGGGTTGACCCGCGTAGAGGGCCCGGCC‑TGCGCCCCCTGGGGCGGGTTAATAGGGGCGCCGGACCCCCGCGAAc                                                                    >  1:5180803/1‑149 (MQ=255)
                                                                                     aTGA‑GGGGGTTTGGCAAAACA‑GCTGCGCGGGTGAACCCGCTCCTACAAGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGa                                                                 <  2:3995086/149‑1 (MQ=255)
                                                                                       tgtgggggTTAGGCCAAAAT‑GGTCCGGGGGTGAACCCGCTCCTCCCCGGGCTTTTGTATCCCTGTTCGGTGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGAc                                                                <  2:2235975/146‑1 (MQ=255)
                                                                                       tgtgggggTTAGGCCAAAAC‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCATGTGTATCCCGGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGAc                                                                <  2:2872071/146‑1 (MQ=255)
                                                                                       tgtgggggTCCGGCCAAAAC‑GCGTCGCGGGTGAACCCGCGCCTACATGGGCTTGTGTACCCCTGGTAGGTGGGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGAc                                                                <  1:3726707/146‑1 (MQ=255)
                                                                                       tgcgggggTTAGGCCAAAAT‑GCTTCGCGGGTGAACCCGCTCCTACACGGGCATGTGTATCCCTGGTTGGTGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGAc                                                                <  2:6477521/146‑1 (MQ=39)
                                                                                            gggTTAGGCCAAAAA‑GCTTCGCGGGTGTACCCGCGCCTAAAAGGGCCGGTGTATCCCGGGGAGGAGCGGGGTCACCCCCGGAGAGGCCCCGTCA‑TGCGCCCCCGTGGGCGGGTTCAACGGGGCGCCGAACCGCCGCGGACAGCACCGAt                                                           >  1:8263758/1‑149 (MQ=255)
                                                                                              gTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCa                                                         <  1:2932007/149‑1 (MQ=255)
                                                                                               ttAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCAAGTGTATACCTGGTAGGAGCGGGTTCAACCCCGAAGAGGCCCGGGCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCaa                                                        >  2:2033360/1‑149 (MQ=39)
                                                                                                   cccAAAAA‑GCTTCGCGGGTGAACCCGCTCCAACACGGGCATGTGTAGCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAgcgc                                                    <  1:6732281/148‑1 (MQ=255)
                                                                                                      aaaaa‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCATGTGTAACCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCcga                                                 <  2:3686786/149‑1 (MQ=255)
                                                                                                        aaa‑GCTTCGGGGGTCACCCCGCTCCTACATGGGCATTTGTATCCCTGGTCGGAGGGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCcgacg                                               <  1:5876655/149‑1 (MQ=255)
                                                                                                         aacgCTTCGCGGGTGAACCCGCTCCTACACGGGCTTGTGTAGCCCGGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCcgacg                                               <  2:1601117/146‑1 (MQ=38)
                                                                                                          a‑GCTTCGCGGGTGAACCCGCTCCCACAAGGGCATGTGTATCCCTGGTAGGAGCGCGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAg                                             <  2:8583967/149‑1 (MQ=255)
                                                                                                                    ggTGAACCCGCTCCTACAAGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTg                                    >  2:7244857/1‑149 (MQ=255)
                                                                                                                    ggTGAACCCGCTCCTACAAGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTg                                    >  2:4918957/1‑149 (MQ=255)
                                                                                                                         ccccGCCCCCCCATGGGCTGGTGTAGCCGGGGTTGGGGGGGGTCCCCCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTg                               <  1:8594565/148‑1 (MQ=255)
                                                                                                                         aCCCGCTCCTACACGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTg                               <  2:5463038/149‑1 (MQ=255)
                                                                                                                         aCCCGCTCCTACAAGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTg                               >  2:843139/1‑149 (MQ=255)
                                                                                                                           ccGCTCCTACAAGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCg                             >  1:6026487/1‑149 (MQ=255)
                                                                                                                            cGCTCCTACAAGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGAGCCGATCAAGCGCCGACGAGGCTGGGTTGGCCTGCGt                            >  1:6476532/1‑149 (MQ=255)
                                                                                                                            cGCTCCTACAAGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGt                            >  1:1918413/1‑149 (MQ=255)
                                                                                                                            cGCTCCTACAAGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGt                            >  1:574267/1‑149 (MQ=255)
                                                                                                                            cGCTCCTACAAGGGCATGTGTATCCCGGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGt                            >  2:8393409/1‑149 (MQ=255)
                                                                                                                              cTCCTACAAGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGttt                          <  2:6738065/149‑1 (MQ=255)
                                                                                                                                ccTACAAGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTc                        <  2:5158882/149‑1 (MQ=255)
                                                                                                                                 cTACACGGGCATGGGTAGCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCa                       <  2:2699879/149‑1 (MQ=255)
                                                                                                                                 cTACAAGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCa                       >  1:5135724/1‑149 (MQ=255)
                                                                                                                                  tACATGGGCATTTTTATCCCTGGTTGGTGGGGGTCCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCAg                      <  1:7867335/149‑1 (MQ=255)
                                                                                                                                  tACAAGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCAg                      >  2:827075/1‑149 (MQ=255)
                                                                                                                                   aCAAGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCAgg                     >  1:3263567/1‑149 (MQ=255)
                                                                                                                                    catgggCTTGTTAATCCCTGGTTGGCGGGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCAggg                    <  1:7759265/146‑1 (MQ=255)
                                                                                                                                    cacgggCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCAggg                    <  2:8714767/146‑1 (MQ=255)
                                                                                                                                    cacgggCATGTGTAGCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCAggg                    <  1:8100299/146‑1 (MQ=255)
                                                                                                                                    cacgggCATGTGTAGCCCGGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCAggg                    <  2:2666910/146‑1 (MQ=255)
                                                                                                                                    cAAGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCAggg                    >  1:4161937/1‑149 (MQ=255)
                                                                                                                                     cagggattgtgTATCCCTGTTAGGCGGGGGTACACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCAGGGt                   <  1:8006918/142‑1 (MQ=255)
                                                                                                                                      cgggCTTGTGTACCCGTGGTGGGGGGGGGTCCCCCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCAGGGTg                  <  1:1074958/148‑1 (MQ=255)
                                                                                                                                      aGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCAGGGTg                  >  1:1602154/1‑149 (MQ=255)
                                                                                                                                      aGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCAGGGTg                  >  1:4719855/1‑149 (MQ=255)
                                                                                                                                        ggCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCAGGGTGCa                >  2:8376623/1‑149 (MQ=255)
                                                                                                                                         gCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCAGGGTGCaa               >  2:6211008/1‑149 (MQ=255)
                                                                                                                                          cATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCAGGGTGCAAc              >  1:1480486/1‑149 (MQ=255)
                                                                                                                                              tgtATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCAGGGTGCAACGGCa          <  2:8917515/149‑1 (MQ=255)
                                                                                                                                              tgtATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCAGGGTGCAACGGCa          >  1:7051265/1‑149 (MQ=255)
                                                                                                                                              tgtATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGGTGTCAGGGTGCAACGGCa          >  2:6364907/1‑149 (MQ=255)
                                                                                                                                                   cccTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCAGGGTGCAACGGCAACAgc     <  2:8016598/149‑1 (MQ=255)
                                                                                                                                                    ccTGGTTGGTGGGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCAGGGTGCAACGGCAACAgcg    <  1:5022550/149‑1 (MQ=255)
                                                                                                                                                     cTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCAGGGTGCAACGGCAACAgcgg   >  2:5179254/1‑149 (MQ=255)
                                                                                                                                                      tGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCAGGGTGCAACGGCAACAgcggc  >  2:5085533/1‑149 (MQ=255)
                                                                                                                                                      tGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCAGGGTGCAACGGCAACAgcggc  >  2:6564690/1‑149 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
ACTGGCCGAAGCGTCGTATCGGAGAGGGATGCTAACGCATGGCGGCCCGTGGACCTAGAGCCGCAGGGGGACTTTGTTGGCCAAAATGA‑GGGGGTTAGGCCAAAAA‑GCTTCGCGGGTGAACCCGCTCCTACAAGGGCATGTGTATCCCTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGGTCA‑TGCGCCCCGTAGGGCGGGTTCATCGGGGCGCCGAACCGCCGCGAACAGACCCGATCAAGCGCCGACGAGGCTCGGTTGGCCTGCGTTTTCAGGGTGCAACGGCAACAGCGGC  >  NC_002947/5729158‑5729454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑