Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,011,323	+T	intergenic (‑258/+192)	PP_0871 ← / ← SBRG_Ecoli_galM	glycine betaine/carnitine/choline ABC transporter permease/SBRG_Ecoli_galM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,011,323	1	.	T	100.0%	15.7 / NA	6	intergenic (‑258/+192)	PP_0871/SBRG_Ecoli_galM	glycine betaine/carnitine/choline ABC transporter permease/SBRG_Ecoli_galM
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (1/5);  total (1/5)

ACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATG‑CTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTG  >  NC_002947/1011225‑1011413                                                                                                    |                                                                                           aCGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATtat                                                        >  1:25439/1‑136 (MQ=255)           gggCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGcga                                              <  2:319139/136‑1 (MQ=255)              cGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGcgacga                                           <  2:15636/136‑1 (MQ=255)                      ttCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAg                                   <  2:25439/136‑1 (MQ=255)                                                       tCGCTGGGTTGCGGGTTTTTGGGCGAGAAATACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTg  <  2:278470/136‑1 (MQ=255)                                                       tCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTg  <  1:354655/136‑1 (MQ=255)                                                                                                    |                                                                                           ACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATG‑CTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTG  >  NC_002947/1011225‑1011413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑