| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,092,447 | 0 | A | C | 25.0% | 33.6 / 11.7 | 20 | intergenic (+268/+650) | PP_1865/aroH | transposase/3‑deoxy‑7‑phosphoheptulonate synthase |
| Reads supporting (aligned to +/- strand): ref base A (5/10); new base C (4/1); total (9/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCT > NC_002947/2092316‑2092579 | aGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAg > 2:249371/1‑136 (MQ=255)aGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAg > 2:226736/1‑136 (MQ=255)aGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAAAGCCAAAg > 2:46975/1‑136 (MQ=255)aGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAAAGCCAAAg > 1:341966/1‑136 (MQ=255)aGGCCCAATCCATGGGGCCTCAGCGCTTGACGGGCCACCATGCCCCTCATCTCCCTCCGCAATGCCAACACACCGCCTCCTGAAGTCGCAAACTGCGTAGGCTGGTCTACCGCGCGCGTAGCAGCAAAAGCCAAAg > 2:330870/1‑136 (MQ=14) ggCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCATCGCCTGAACTACCGCGCGCTTAGAAGCAAAAGCCAAAGc > 1:284514/1‑136 (MQ=14) tccatGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAAAGCCAAAGCaagagca < 1:46975/136‑4 (MQ=14) catGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGa > 2:293234/1‑136 (MQ=255) catGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGa > 2:203395/1‑136 (MQ=255) ttGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATcggccg < 1:249371/136‑1 (MQ=255) tGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATcggccgg < 1:335790/136‑1 (MQ=255) ggCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCAccg < 1:171034/136‑1 (MQ=255) aTCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCAccgccg < 1:226736/136‑1 (MQ=255) gCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGccc > 1:213922/1‑136 (MQ=255) ccTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCAt < 2:213922/136‑1 (MQ=255) cTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTg < 1:351949/136‑1 (MQ=255) gTCGCCTGAACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCt < 2:365007/136‑1 (MQ=255) gcgcTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTgg < 1:169539/136‑1 (MQ=255) gcTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGgaa < 1:293234/136‑1 (MQ=255) gcTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGgaa < 1:203395/136‑1 (MQ=255) atgaaaaGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGTACGCCGttgct < 1:368784/133‑1 (MQ=255) | AGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCT > NC_002947/2092316‑2092579 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |