Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,015,840	T→A	intergenic (‑67/‑701)	SBRG_Ecoli_galE ← / → prfC	SBRG_Ecoli_galE/peptide chain release factor 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,015,840	0	T	A	100.0%	26.0 / NA	9	intergenic (‑67/‑701)	SBRG_Ecoli_galE/prfC	SBRG_Ecoli_galE/peptide chain release factor 3
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (6/3);  total (6/3)

AATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACATGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTAAGTGATTATAAAACAGAGGGTTTATGAATGATTGCGCTTTTTATCTGAAAAA  >  NC_002947/1015738‑1015949                                                                                                       |                                                                                                              aaTGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACAAGGAATAAATTAGTGGAATCGTTTACACAAGAAt                                                                              >  2:261035/1‑136 (MQ=255)                      aaCCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACAAGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGa                                                         >  2:263339/1‑136 (MQ=255)                         cAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACAAGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTa                                                      <  1:261035/136‑1 (MQ=255)                                       ttCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACAAGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTAAGTGATTATAAAAc                                        <  2:386182/136‑1 (MQ=255)                                              cATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACAAGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTAAGTGATTATAAAACAGAGGGt                                 >  2:215376/1‑136 (MQ=255)                                              cATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACAAGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTAAGTGATTATAAAACAGAGGGt                                 >  2:265940/1‑136 (MQ=255)                                                        aTGGTATTAAATAACCATAGCATAACAAAGATGCGAATAGTGTGACAATGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTAAGTGATTATAAAACAGAGGGTTTatgaatga                       <  1:227724/136‑1 (MQ=255)                                                           gTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACAAGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTAAGTGATTATAAAACAGAGGGTTTATGAATGATTg                    >  2:351637/1‑136 (MQ=255)                                                                             aTAACAAAGATGCGAAAAGTGTGACAAGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTAAGTGATTATAAAACAGAGGGTTTATGAATGATTGCGCTTTTTATCTGaaaaa  >  2:1544/1‑136 (MQ=255)                                                                                                       |                                                                                                              AATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACATGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTAAGTGATTATAAAACAGAGGGTTTATGAATGATTGCGCTTTTTATCTGAAAAA  >  NC_002947/1015738‑1015949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑