Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,855,277 Δ1 bp 13.8% coding (977/1842 nt) ilvD → dihydroxy‑acid dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,855,2770G.13.8% 89.9 / 8.4 29coding (977/1842 nt)ilvDdihydroxy‑acid dehydratase
Reads supporting (aligned to +/- strand):  ref base G (12/13);  new base . (2/2);  total (14/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCACCTGCTGGCAGCAGCACAGGAAGGCGAAGTGGCGTTCGACCTGCGCGACATCGACCGCCTGTCACGCAAAGTGCCGCAGCTGTGCAAAGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTCCACCGCGCCGGCGGCATCTTCAGCATCCTCGGTTCGCTGGCCCGTGGCGGCCTGCTGCACACCGACCTGCCGACCGTGCACAGCCGCAGCATGGAAGAAGCCATCGCCAAATGGGACATCACCCAG  >  NC_002947/5855143‑5855398
                                                                                                                                      |                                                                                                                         
gCACCTGCTGGCAGCAGCACAGGAAGGCGAAGTGGCGTTCGACCTGCGCGACATCGACCGCCTGTCACGCAAAGTGCCGCAGCTGTGCAAAGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTCCACcgc                                                                                                                          >  1:409714/1‑136 (MQ=255)
 caactgctgTCTGCAGCACAGTAAGGCGAAGTGGCGTTCTACCTTCGCGTCATCGACCGCCTGTCACTCAAAGTGCCGCAGCTGTGCAAATGGGCGCCGAAAATACAGAAGTACCACATGGAAGACGTCCACagcg                                                                                                                         <  2:357789/133‑1 (MQ=255)
 cACCTGCTGGCAGCAGCACAGGAAGGCGAAGTGGCGTTCGACCTGCGCGACATCGACCGCCTGTCACGCAAAGTGCCGCAGCTGTGCAAAGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTCCACcgcg                                                                                                                         <  2:286874/136‑1 (MQ=255)
    ctgctgGCAGCAGCACAGGAAGGCGAAGTGGCGTTCGACCTGCGCGACATCGACCGCCTGTCACGCAAAGTGCCGCAGCTGTGCAAAGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTCCACCGCGCcg                                                                                                                      <  1:24363/136‑1 (MQ=255)
     tgctgGCAGCAGCACAGGAAGGCGAAGTGGCGTTCGACCTGCGCGACATCGACCGCCTGTCACGCAAAGTGCCGCAGCTGTGCAAAGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTCCACCGCGCcgg                                                                                                                     >  2:39445/1‑136 (MQ=255)
     tgctgGCAGCAGCACAGGAAGGCGAAGTGGCGTTCGACCTGCGCGACATCGACCGCCTGTCACGCAAAGTGCCGCAGCTGTGCAAAGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTCCACCGCGCcgg                                                                                                                     >  2:13712/1‑136 (MQ=255)
         ggCAGCAGCACAGGAAGGCGAAGTGGCGTTCGACCTGCGCGACATCGACCGCCTGTCACGCAAAGTGCCGCAGCTGTGCAAAGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTCCACCGCGCCggcggc                                                                                                                 >  1:342020/1‑136 (MQ=255)
         ggCAGCAGCACAGGAAGGCGAAGTGGCGTTCGACCTGCGCGACATCGACCGCCTGTCACGCAAAGTGCCGCAGCTGTGCAAAGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTCCACCGCGCCggcggc                                                                                                                 >  1:180153/1‑136 (MQ=255)
         ggCAGCAGCACAGGAAGGCGAAGTGGCGTTCGACCTGCGCGACATCGACCGCCTGTCACGCAAAGTGCCGCAGCTGTGCAAAGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTCCACCGCGCCggcggc                                                                                                                 >  1:542229/1‑136 (MQ=255)
            agcagcaCAGGAAGGCGAAGTGGCGTTCGACCTGCGCGACATCGACCGCCTGTCACGCAAAGTGCCGCAGCTGTGCAAAGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTCCACCGCGCCGGCGGCATc                                                                                                              <  2:409714/136‑1 (MQ=255)
                         ggCGAAGTGGCGTTCGACCTGCGCGACATCGACCGCCTGTCACGCAAAGTGCCGCAGCTGTGCAAAGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTCCACCGCGCCGGCGGCATCTTCAGCATCCTCg                                                                                                 >  2:428009/1‑136 (MQ=255)
                            gAAGTGGCGTTCGACCTGCGCGACATCGACCGCCTGTCACGCAAAGTGCCGCAGCTGTGCAAAGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTCCAC‑‑CGCCGGCGGCATCTTCAGCATCCTCGGTTCg                                                                                            >  2:290063/1‑136 (MQ=255)
                            gAAGTGGCGTTCGACCTGCGCGACATCGACCGCCTGTCACGCAAAGTGCCGCAGCTGTGCAAAGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTCCAC‑‑CGCCGGCGGCATCTTCAGCATCCTCGGTTCg                                                                                            >  2:287321/1‑136 (MQ=255)
                                        caCCTGCGCGACATCGACCGCCTGTCACGCAAAGTGCCGCAGCTGTGCAAAGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTCCACCGCGCCGGCGGCATCTTCAGCATCCTCGGTTCGCTGGCCCGTg                                                                                  <  1:188066/135‑1 (MQ=255)
                                                 gACATCGACCGCCTGTCACGCAAAGTGCCGCAGCTGTGCAAAGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTCCACCGCGCCGGCGGCATCTTCAGCATCCTCGGTTCGCTGGc                                                                                       >  1:500656/1‑122 (MQ=255)
                                                 gACATCGACCGCCTGTCACGCAAAGTGCCGCAGCTGTGCAAAGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTCCACCGCGCCGGCGGCATCTTCAGCATCCTCGGTTCGCTGGc                                                                                       <  2:500656/122‑1 (MQ=255)
                                                                           gCCGCAGCTGTGCAAAGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTCCAC‑‑CGCCGGCGGCATCTTCAGCATCCTCGGTTCGCTGGCCCGTGGCGGCCTGCTGCACACCGACCTGCCGACCGTGCACAg                                             <  1:290063/136‑1 (MQ=255)
                                                                           gCCGCAGCTGTGCAAAGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTCCAC‑‑CGCCGGCGGCATCTTCAGCATCCTCGGTTCGCTGGCCCGTGGCGGCCTGCTGCACACCGACCTGCCGACCGTGCACAg                                             <  1:287321/136‑1 (MQ=255)
                                                                             cGCAGCTGTGCAAAGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTCCACCGCGCCGGCGGCATCTTCAGCATCCTCGGTTCGCTGGCCCGTGGCGGCCTGCTGCACACCGACCTGCCGACCGTGCACAg                                             <  1:529888/136‑1 (MQ=255)
                                                                             cGCAGCTGTGCAAAGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTCCACCGCGCCGGCGGCATCTTCAGCATCCTCGGTTCGCTGGCCCGTGGCGGCCTGCTGCACACCGACCTGCCGACCGTGCACAg                                             <  1:222250/136‑1 (MQ=255)
                                                                                     tgCAAAGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTCCACCGCGCCGGCGGCATGCTTAGCCTCCTAGGTTACCGTGCCCGTTGCGGCCTGCTTCACCCCGCCCATCCGACCGTGCCCAGccccctca                                     >  1:20702/1‑130 (MQ=255)
                                                                                     tgCAAAGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTCCACCGCGCCGGCGGCATCTTCAGCATCCTCGGTTCGCTGGCCCGTGGCGGCCTGCTGCACACCGACCTGCCGACCGTGCACAGCCgcagca                                     >  1:223472/1‑136 (MQ=255)
                                                                                       cAAAGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTCCACCGCGCCGGCGGCATCTTCAGCATCCTCGGTTCGCTGGCCCGTGGCGGCCTGCTGCACACCGACCTGCCGACCGTGCACAGCCGCAGCATg                                   <  1:228550/136‑1 (MQ=255)
                                                                                                         ccAGAAGTACCACATGGAAGACGTCCACCGCGCCGGCGGCATCTTCAGCATCCTCGGTTCGCTGGCCCGTGGCGGCCTGCTGCACACCGACCTGCCGACCGTGCACAGCCGCAGCATGGAAGAAGCCATCGCCaaa                 >  2:323644/1‑136 (MQ=255)
                                                                                                            gAAGTACCACATGGAAGACGTCCACCGCGCCGGCGGCATCTTCAGCATCCTCGGTTCGCTGGCCCGTGGCGGCCTGCTGCACACCGACCTGCCGACCGTGCACAGCCGCAGCATGGAAGAAGCCATCGCCAAATgg              <  1:547468/136‑1 (MQ=255)
                                                                                                            gAAGTACCACATGGAAGACGTCCACCGCGCCGGCGGCATCTTCAGCATCCTCGGTTCGCTGGCCCGTGGCGGCCTGCTGCACACCGACCTGCCGACCGTGCACAGCCGCAGCATGGAAGAAGCCATCGCCAAATgg              <  1:445935/136‑1 (MQ=255)
                                                                                                                      aTGGAAGACGTCCACCGCGCCGGCGGCATCTTCAGCATCCTCGGTTCGCTGGCCCGTGGCGGCCTGCTGCACACCGACCTGCCGACCGTGCACAGCCGCAGCATGGAAGAAGCCATCGCCAAATGGGACATCAccc    >  2:110706/1‑136 (MQ=255)
                                                                                                                      aTGGAAGACGTCCACCGCGCCGGCGGCATCTTCAGCATCCTCGGTTCGCTGGCCCGTGGCGGCCTGCTGCACACCGACCTGCCGACCGTGCACAGCCGCAGCATGGAAGAAGCCATCGCCAAATGGGACATCAccc    >  2:501802/1‑136 (MQ=255)
                                                                                                                        ggtaGACGTCCACCGCGCCGGCGGCATCTTCAGCATCCTCGGTTCGCTGGCCCGTGGCGGCCTGCTGCACACCGACCTGCCGACCGTGCACAGCCGCAGCATGGAAGAAGCCATCGCCAAATGGGACATCACCCAg  <  2:180153/133‑1 (MQ=255)
                                                                                                                        ggAAGACGTCCACCGCGCCGGCGGCATCTTCAGCATCCTCGGTTCGCTGGCCCGTGGCGGCCTGCTGCACACCGACCTGCCGACCGTGCACAGCCGCAGCATGGAAGAAGCCATCGCCAAATGGGACATCACCCAg  <  2:342020/136‑1 (MQ=255)
                                                                                                                        ggAAGACGTCCACCGCGCCGGCGGCATCTTCAGCATCCTCGGTTCGCTGGCCCGTGGCGGCCTGCTGCACACCGACCTGCCGACCGTGCACAGCCGCAGCATGGAAGAAGCCATCGCCAAATGGGACATCACCCAg  <  1:501802/136‑1 (MQ=255)
                                                                                                                        ggAAGACGTCCACCGCGCCGGCGGCATCTTCAGCATCCTCGGTTCGCTGGCCCGTGGCGGCCTGCTGCACACCGACCTGCCGACCGTGCACAGCCGCAGCATGGAAGAAGCCATCGCCAAATGGGACATCACCCAg  <  2:542229/136‑1 (MQ=255)
                                                                                                                                      |                                                                                                                         
GCACCTGCTGGCAGCAGCACAGGAAGGCGAAGTGGCGTTCGACCTGCGCGACATCGACCGCCTGTCACGCAAAGTGCCGCAGCTGTGCAAAGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTCCACCGCGCCGGCGGCATCTTCAGCATCCTCGGTTCGCTGGCCCGTGGCGGCCTGCTGCACACCGACCTGCCGACCGTGCACAGCCGCAGCATGGAAGAAGCCATCGCCAAATGGGACATCACCCAG  >  NC_002947/5855143‑5855398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑