| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,914,939 | C→T | 16.0% | G164D (GGC→GAC) | aprDA ← | alkaline protease secretion ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,914,939 | 0 | C | T | 16.0% | 58.5 / 4.1 | 25 | G164D (GGC→GAC) | aprDA | alkaline protease secretion ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (10/11); new base T (2/2); total (12/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGTTGCGCAGGTTGTTGTTGGCGTAACTGGCCGACGACATGGCTGCCTGGTTGGCTTCGGCCAAGGGTTTTTGCGTGGCCTTTTCAGTGAGGTAGGCCAGGCCGACGAGGATCAGCGAGCCAATCGTGGTGACCAGCCCGAGCCACGGGTGGATCAGGTAGCAAACCAGCAGGTAGATCGGTGTCCAGGGGGCGTCGAAGAACGCGAACAGGCCGTTACCGGTGAGAAACTGCCGTACCTGCGCCAAGTCC > NC_002947/2914819‑2915070 | gCGTTGCGCAGGTTGTTGTTGGCGTAACTGGCCGACGACATGGCTGCCTGGTTGGCTTCGGCCAAGGGTTTTTGCGTGGCCTTTTCAGTGAGGTAGGCCAGGCCGACGAGGATCAGCGAGCCAATCGTGGTGACCa < 1:251879/136‑1 (MQ=255)gCGTTGCGCAGGTTGTTGTTGGCGTAACTGGCCGACGACATGGCTGCCTGGTTGGCTTCGGCCAAGGGTTTTTGCGTGGCCTTTTCAGTGAGGTAGGCCAGGCCGACGAGGATCAGCGAGCCAATCGTGGTGACCa < 1:270630/136‑1 (MQ=255) ttGCGCAGGTTGTTGTTGGCGTAACTGGCCGACGACATGGCTGCCTGGTTGGCTTCGGCCAAGGGTTTTTGCGTGGCCTTTTCAGTGAGGTAGGCCAGGCCGACGAGGATCAGCGAGCCAATCGTGGTGACCAGcc > 2:302544/1‑136 (MQ=255) tGCGCAGGTTGTTGTTGGCGTAACTGGCCGACGACATGGCTGCCTGGTTGGCTTCGGCCAAGGGTTTTTGCGTGGCCTTTTCAGTGAGGTAGGCCAGGCCGACGAGGATCAGCGAGTCAATCGTGGTGACCAGccc > 2:127714/1‑136 (MQ=255) tGCGCAGGTTGTTGTTGGCGTAACTGGCCGACGACATGGCTGCCTGGTTGGCTTCGGCCAAGGGTTTTTGCGTGGCCTTTTCAGTGAGGTAGGCCAGGCCGACGAGGATCAGCGAGTCAATCGTGGTGACCAGccc > 2:125177/1‑136 (MQ=255) aGGTTGTTGTTGGCGTAACTGGCCGACGACATGGCTGCCTGGTTGGCTTCGGCCAAGGGTTTTTGCGTGGCCTTTTCAGTGAGGTAGGCCAGGCCGACGAGGATCAGCGAGCCAATCGTGGTGACCAGCCCGAGcc > 1:121861/1‑136 (MQ=255) aGGTTGTTGTTGGCGTAACTGGCCGACGACATGGCTGCCTGGTTGGCTTCGGCCAAGGGTTTTTGCGTGGCCTTTTCAGTGAGGTAGGCCAGGCCGACGAGGATCAGCGAGCCAATCGTGGTGACCAGCCCGAGcc > 1:3600/1‑136 (MQ=255) tAACTGGCCGACGACATGGCTGCCTGGTTGGCTTCGGCCAAGGGTTTTTGCGTGGCCTTTTCAGTGAGGTAGGCCAGGCCGACGAGGATCAGCGAGCCAATCGTGGTGACCAGCCCGAGCCACGGGTGGATCAGGt > 1:244949/1‑136 (MQ=255) cgacgaCATGGCTGCCTGGTTGGCTTCGGCCAAGGGTTTTTGCGTGGCCTTTTCAGTGAGGTAGGCCAGGCCGACGAGGATCAGCGAGTCAATCGTGGTGACCAGCCCGAGCCACGGGTGGATCAGGTAGCAAAcc < 1:125177/136‑1 (MQ=255) cgacgaCATGGCTGCCTGGTTGGCTTCGGCCAAGGGTTTTTGCGTGGCCTTTTCAGTGAGGTAGGCCAGGCCGACGAGGATCAGCGAGTCAATCGTGGTGACCAGCCCGAGCCACGGGTGGATCAGGTAGCAAAcc < 1:127714/136‑1 (MQ=255) cgacgaCATGGCTGCCTGGTTGGCTTCGGCCAAGGGTTTTTGCGTGGCCTTTTCAGTGAGGTAGGCCAGGCCGACGAGGATCAGCGAGCCAATCGTGGTGACCAGCCCGAGCCACGGGTGGATCAGGTAGCAAAcc < 1:241224/136‑1 (MQ=255) cgacgaCATGGCTGCCTGGTTGGCTTCGGCCAAGGGTTTTTGCGTGGCCTTTTCAGTGAGGTAGGCCAGGCCGACGAGGATCAGCGAGCCAATCGTGGTGACCAGCCCGAGCCACGGGTGGATCAGGTAGCAAAcc < 2:383756/136‑1 (MQ=255) tGGCTGCCTGGTTGGCTTCGGCCAAGGGTTTTTGCGTGGCCTTTTCAGTGAGGTAGGCCAGGCCGACGAGGATCAGCGAGCCAATCGTGGTGACCAGCCCGAGCCACGGGTGGATCAGGTAGCAAACCAGCAGGTa < 2:310237/136‑1 (MQ=255) gCTGCCTGGTTGGCTTCGGCCAAGGGTTTTTGCGTGGCCTTTTCAGTGAGGTAGGCCAGGCCGACGAGGATCAGCGAGCCAATCGTGGTGACCAGCCCGAGCCACGGGTGGATCAGGTAGCAAACCAGCAGGTAGa < 1:302544/136‑1 (MQ=255) tGGTTGGCTTCGGCCAAGGGTTTTTGCGTGGCCTTTTCAGTGAGGTAGGCCAGGCCGACGAGGATCAGCGAGCCAATCGTGGTGACCAGCCCGAGCCACGGGTGGATCAGGTAGCAAACCAGCAGGTAGATCGgtg < 2:121861/136‑1 (MQ=255) ttGGCTTCGGCCAAGGGTTTTTGCGTGGCCTTTTCAGTGAGGTAGGCCAGGCCGACGAGGATCAGCGAGCCAATCGTGGTGACCAGCCCGAGCCACGGGTGGATCAGGTAGCAAACCAGCAGGTAGATCGgtgt > 1:310605/1‑134 (MQ=255) ttGGCTTCGGCCAAGGGTTTTTGCGTGGCCTTTTCAGTGAGGTAGGCCAGGCCGACGAGGATCAGCGAGCCAATCGTGGTGACCAGCCCGAGCCACGGGTGGATCAGGTAGCAAACCAGCAGGTAGATCGgtgt < 2:310605/134‑1 (MQ=255) ggCTTCGGCCAAGGGTTTTTGCGTGGCCTTTTCAGTGAGGTAGGCCAGGCCGACGAGGATCAGCGAGCCAATCGTGGTGACCAGCCCGAGCCACGGGTGGATCAGGTAGCAAACCAGCAGGTAGATCGGTGTCCAg > 1:391193/1‑136 (MQ=255) gTAGGCCAGGCCGACGAGGATCAGCGAGCCAATCGTGGTGACCAGCCCGAGCCACGGGTGGATCAGGTAGCAAACCAGCAGGTAGATCGGTGTCCAGGGGGCGTCGAAGAAcgc > 2:260789/1‑114 (MQ=255) gTAGGCCAGGCCGACGAGGATCAGCGAGCCAATCGTGGTGACCAGCCCGAGCCACGGGTGGATCAGGTAGCAAACCAGCAGGTAGATCGGTGTCCAGGGGGCGTCGAAGAAcgc < 1:260789/114‑1 (MQ=255) aggccGACGAGGATCAGCGAGCCAATCGTGGTGACCAGCCCGAGCCACGGGTGGATCAGGTAGCAAACCAGCAGGTAGATCGGTGTCCAGGGGGCGTCGAAGAACGCGAACAGGCCGTTACCGGTGAGAAACTGcc > 2:521333/1‑136 (MQ=255) gaGGATCAGCGAGCCAATCGTGGTGACCAGCCCGAGCCACGGGTGGATCAGGTAGCAAACCAGCAGGTAGATCGGTGTCCAGGGTGCGTCGAAGAACGCGAACAGGCCGTTACCGGTGAGAAACTGCCGTACCTgc < 2:355594/136‑1 (MQ=255) ggATCAGCGAGCCAATCGTGGTGACCAGCCCGAGCCACGGGTGGATCAGGTAGCAAACCAGCAGGTAGATCGGTGTCCAGGGGGCGTCGAAGAACGCGAACAGGCCGTTACCGGTGAGAAACTGCCGTACCTgcgc > 1:146026/1‑136 (MQ=255) ggATCAGCGAGCCAATCGTGGTGACCAGCCCGAGCCACGGGTGGATCAGGTAGCAAACCAGCAGGTAGATCGGTGTCCAGGGGGCGTCGAAGAACGCGAACAGGCCGGTACCGGTGAGAAACTGCCGTACCTgcgc > 1:545521/1‑136 (MQ=255) ctaGCCAATCGTGGTGACCAGCCCGAGCCACGGGTGGATCAGGTAGCAAACCAGGAGGTAGATCGGTGTCCAGGGGTCGTCGAAGAACGCGAACAGGCCGTTACCGGTGAGAAACTGCCGTACCTGCGCCAAGTcc < 2:4197/134‑1 (MQ=255) | GCGTTGCGCAGGTTGTTGTTGGCGTAACTGGCCGACGACATGGCTGCCTGGTTGGCTTCGGCCAAGGGTTTTTGCGTGGCCTTTTCAGTGAGGTAGGCCAGGCCGACGAGGATCAGCGAGCCAATCGTGGTGACCAGCCCGAGCCACGGGTGGATCAGGTAGCAAACCAGCAGGTAGATCGGTGTCCAGGGGGCGTCGAAGAACGCGAACAGGCCGTTACCGGTGAGAAACTGCCGTACCTGCGCCAAGTCC > NC_002947/2914819‑2915070 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |