| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,143,420 | G→T | 19.9% | Q189H (CAG→CAT) | PP_2754 → | OprD family outer membrane porin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,143,420 | 0 | G | T | 19.9% | 51.6 / 2.7 | 20 | Q189H (CAG→CAT) | PP_2754 | OprD family outer membrane porin |
| Reads supporting (aligned to +/- strand): ref base G (5/11); new base T (2/2); total (7/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
CTTCGACACCGAGCTGCGCTATGGCGACGTCAGCCCGACCAGCCCGGTGCTGCACGCCAGCGACATTCGCCTGCTGCCGCAGACGCTGCGCGGCTTCATCTTCAACAACAACTCGCTGGATGGCCTGAGCCTGCAGGGCGGCAAGCTGGAGTCGAGCAGTGACCGCAACGCCAGCAACCACCGTGGTGATCTCGGCACCGTCTATGCCGGGCGTATCAAGGGCGCCAACGATGTGGTGTACCTGGGTGGCGATTACCGGTTCAACGACCA > NC_002947/3143285‑3143554 | cTTCGACACCGAGCTGCGCTATGTCGACGTCAGCCCGACCAGCAAGGTGCTGCACGCCAGCGACATTCGCCTGCTGCCGCAGACGCTGAGCGGCTTCATCATCAACAACAACACGCTGGATGGCCTGATCCTGCAg > 1:425250/1‑136 (MQ=255) cgtgcggcgcTAGGGCGACGTCAGCCCGACCTGCCCGGTGCTGCACGCCAGCGACATTCGCCTGCTGCCGCAGACGCTGCGCGGCTTCATCTTCAACAACAACTCGCTGGATGGCCTGAGCCTGCAGGGCGGCAAg < 2:475322/130‑1 (MQ=255) gtGCGCTATGGCGACGTCAGCCCGACCAGCCCGGTGCTGCACGCCAGCGACATTCGCCTGCTGCCGCAGACGCTGCGCGGCTTCATCTTCAACAACAACTCGCTGGATGGCCTGAGCCTGCAGGGCGGCAAGCTgg < 2:425250/135‑1 (MQ=255) tGCGCTATGGCGACGTCAGCCCGACCAGCCCGGTGCTGCACGCCAGCGACATTCGCCTGCTGCCGCAGACGCTGCGCGGCTTCATCTTCAACAACAACTCGCTGGATGGCCTGAGCCTGCAGGGCGGCAAGCTGGa > 2:501314/1‑136 (MQ=255) tCAGCCCGACCAGCCCGGTGCTGCACGCCAGCGACATTCGCCTGCTGCCGCAGACGCTGCGCGGCTTCATCTTCAACAACAACTCGCTGGATGGCCTGAGCCTGCATGGCGGCAAGCTGGAGTCGAGCAGTGACCg > 2:287936/1‑136 (MQ=255) gACCAGCCCGGTGCTGCACGCCAGCGACATTCGCCTGCTGCCGCAGACGCTGCGCGGCTTCATCTTCAACAACAACTCGCTGGATGGCCTGAGCCTGCAGGGCGGCAAGCTGGAGTCGAGCAGTGACCGCAACGcc < 1:323021/136‑1 (MQ=255) gCCCGGTGCTGCACGCCAGCGACATTCGCCTGCTGCCGCAGACGCTGCGCGGCTTCATCTTCAACAACAACTCGCTGGATGGCCTGAGCCTGCAGGGCGGCAAGCTGGAGTCGAGCAGTGACCg > 2:514253/1‑124 (MQ=255) gCCCGGTGCTGCACGCCAGCGACATTCGCCTGCTGCCGCAGACGCTGCGCGGCTTCATCTTCAACAACAACTCGCTGGATGGCCTGAGCCTGCAGGGCGGCAAGCTGGAGTCGAGCAGTGACCg < 1:514253/124‑1 (MQ=255) gCCCGGTGCTGCACGCCAGCGACATTCGCCTGCTGCCGCAGACGCTGCGCGGCTTCATCTTCAACAACAACTCGCTGGATGGCCTGAGCCTGCAGGGCGGCAAGCTGGAGTCGAGCAGTGACCGCAACGCCAGCaa < 1:308658/136‑1 (MQ=255) ccGCAGACGCTGCGCGGCTTCATCTTCAACAACAACTCGCTGGATGGCCTGAGCCTGCATGGCGGCAAGCTGGAGTCGAGCAGTGACCGCAACGCCAGCAACCACCGTGGTGATCTCGGCACCGTCTATGCCGGGc > 1:363759/1‑136 (MQ=255) tGCGCGGCTTCATCTTCAACAACAACTCGCTGGATGGCCTGAGCCTGCAGGGCGGCAAGCTGGAGTCGAGCAGTGACCGCAACGCCAGCAACCACCGTGGTGATCTCGGCACCGTCTATGCCGGGCGTATCAAggg < 2:206154/136‑1 (MQ=255) cgGCTTCATCTTCAACAACAACTCGCTGGATGGCCTGAGCCTGCAGGGCGGCAAGCTGGAGTCGAGCAGTGACCGCAACGCCAGCAACCACCGTGGTGATCTCGGCACCGTCTATGCCGGGCGTATCAAGGGCGcc > 2:249605/1‑136 (MQ=255) ttCATCTTCAACAACAACTCGCTGGATGGCCTGAGCCTGCAGGGCGGCAAGCTGGAGTCGAGCAGTGACCGCAACGCCAGCAACCACCGTGGTGATCTCGGCACCGTCTATGCCGGGCGTATCAAGGGCGCCAACg < 1:249605/136‑1 (MQ=255) cATCTTCAACAACAACTCGCTGGATGGCCTGAGCCTGCAGGGCGGCAAGCTGGAGTCGAGCAGTGACCGCAACGCCAGCAACCACCGTGGTGATCTCGGCACCGTCTATGCCGGGCGTATCAAGGGCGCCAACGAt < 2:193914/136‑1 (MQ=255) aacaacTCGCTGGATGGCCTGAGCCTGCAGGGCGGCAAGCTGGAGTCGAGCAGTGACCGCAACGCCAGCAACCACCGTGGTGATCTCGGCACCGTCTATGCCGGGCGTATCAAGGGCGCCAACGATGTGGTGTAcc > 1:356974/1‑136 (MQ=255) aacTCGCTGGATGGCCTGAGCCTGCAGGGAGGCAAGCTGGAGTCGAGCAGTGACCGCAACGCCAGCAACCACCGTGGTGATCTCGGCACCTTCTATGCCGGGCGTATCAAGGGCGCCAACGATGTTGTGTACCTgg < 2:334231/136‑1 (MQ=255) ggATGGCCTGAGCCTGCATGGCGGCAAGCTGGAGTCGAGCAGTGACCGCAACGCCAGCAACCACCGTGGTGATCTCGGCACCGTCTATGCCGGGCGTATCAAGGGCGCCAACGATGTGGTGTACCTGGGTGGCGAt < 2:363759/136‑1 (MQ=255) ggATGGCCTGAGCCTGCATGGCGGCAAGCTGGAGTCGAGCAGTGACCGCAACGCCAGCAACCACCGTGGTGATCTCGGCACCGTCTATGCCGGGCGTATCAAGGGCGCCAACGATGTGGTGTACCTGGGTGGCGAt < 1:287936/136‑1 (MQ=255) tGAGCCTGCAGGGCGGCAAGCTGGAGTCGAGCAGTGACCGCAACGCCAGCAACCACCGTGGTGATCTCGGCACCGTCTATGCCGGGCGTATCAAGGGCGCCAACGATGTGGTGTACCTGGGTGGCGATTACCGGtt > 2:460445/1‑136 (MQ=255) aGGGCGGCAAGCTGGAGTCGAGCAGTGACCGCAACGCCAGCAACCACCGTGGTGATCTCGGCACCGTCTATGCCGGGCGTATCAAGGGCGCCAACGATGTGGTGTACCTGGGTGGCGATTACCGGTTCAACGACCa < 1:446389/136‑1 (MQ=255) aGGGCGGCAAGCTGGAGTCGAGCAGTGACCGCAACGCCAGCAACCACCGTGGTGATCTCGGCACCGTCTATGCCGGGCGTATCAAGGGCGCCAACGATGTGGTGTACCTGGGTGGCGATTACCGGTTCAACGACCa < 1:100014/136‑1 (MQ=255) | CTTCGACACCGAGCTGCGCTATGGCGACGTCAGCCCGACCAGCCCGGTGCTGCACGCCAGCGACATTCGCCTGCTGCCGCAGACGCTGCGCGGCTTCATCTTCAACAACAACTCGCTGGATGGCCTGAGCCTGCAGGGCGGCAAGCTGGAGTCGAGCAGTGACCGCAACGCCAGCAACCACCGTGGTGATCTCGGCACCGTCTATGCCGGGCGTATCAAGGGCGCCAACGATGTGGTGTACCTGGGTGGCGATTACCGGTTCAACGACCA > NC_002947/3143285‑3143554 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |