| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,292,711 | C→A | 17.4% | D132Y (GAC→TAC) | xthA ← | exodeoxyribonuclease III/apurinic/apyrimidinic endodeoxyribonuclease VI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,292,711 | 0 | C | A | 17.4% | 64.0 / 2.4 | 23 | D132Y (GAC→TAC) | xthA | exodeoxyribonuclease III/apurinic/apyrimidinic endodeoxyribonuclease VI |
| Reads supporting (aligned to +/- strand): ref base C (8/11); new base A (2/2); total (10/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.33e-01 | |||||||||||
TCAGCCAGCGTTTGGCGTTGTCCGGGCCAATGCCGATATCACAGTCTTGCGGCGAGATGTTCATGTCGCCCATCACCAGCACTGGCTGGTCGTTGCGGAACTGGCTTTCCAGCAGTGCCTGCAGGTCGCTGTAGAAGCGCTGCTTGGCCGGGAACTTGGTGGGGTGGTCGCGGCTTTCGCCTTGGGGGAAATAGCCGTTCATGATGGTGATCGGGCTGCCGTTGGCATCGGCAAAGGTACCCCAGATGAAGCGGCGCTGG > NC_002947/3292585‑3292844 | tCAGCCAGCGTTTGGCGTTGTCCGGGCCAATGCCGATATCACAGTCTTGCGGCGAGATGTTCATGTCGCCCATCACCAGCACTGGCTGGTCGTTGCGGAACTGGCTTTCCAGCAGTGCCTGCAGGTCGCTGTAGaa > 2:456290/1‑136 (MQ=255)tCAGCCAGCGTTTGGCGTTGTCCGGGCCAATGCCGATATCACAGTCTTGCGGCGAGATGTTCATGTCGCCCATCACCAGCACTGGCTGGTCGTTGCGGAACTGGCTTTCCAGCAGTGCCTGCAGGTCGCTGTAGaa < 1:352674/136‑1 (MQ=255) gCGTTGTCCGGGCCAATGCCGATATCACAGTCTTGCGGCGAGATGTTGATGTCGCCCATCACCAGCACTGGCTGGTCGTTGCGGAACTGGCTTTCCAGCAGTGCCTGCAGGTCGCTGTAGAAGCGCTGCTTGGCCg < 1:508568/136‑1 (MQ=255) gCCGATATCACAGTCTTGCGGCGAGATGTTCATGTCGCCCATCACCAGCACTGGCTGGTCGTTGCGGAACTGGCTTTCCAGCAGTGCCTGCAGGTCGCTGTAGAAGCGCTGCTTGGCCGGGAACTTggtggggtgg < 1:176315/136‑1 (MQ=255) tGCGGCGAGATGTTCATGTCGCCCATCACCAGCACTGGCTGGTCGTTGCGGAACTGGCTTTCCAGCAGTGCCTGCAGGTCGCTGTAGAAGCGCTGCTTGGCCGGGAACTTGGTGGGGTGGTCGCGGCTTTCGCCtt > 2:405890/1‑136 (MQ=255) tGCGGCGAGATGTTCATGTCGCCCATCACCAGCACTGGCTGGTCGTTGCGGAACTGGCTTTCCAGCAGTGCCTGCAGGTCGCTGTAGAAGCGCTGCTTGGCCGGGAACTTGGTGGGGTGGTCGCGGCTTTCGCCtt > 2:116372/1‑136 (MQ=255) tCGCCCATCACCAGCACTGGCTGGTCGTTGCGGAACTGGCTTTCCAGCAGTGCCTGCAGGTCGCTGTAGAAGCGCTGCTTGGCCGGGAACTTGGTGGGGTGGTCGCGGCTTTCGCCTTGGGGGAAATAGCCGTTCa < 2:448833/136‑1 (MQ=255) cAGCACTGGCTGGTCGTTGCGGAACTGGCTTTCCAGCAGTGCCTGCAGGTCGCTGTAGAAGGGCTGCTTGGCCGGGAACTTGGTGGGGTGGTCGCGGCTTTCGCCTTGGGGGAAATAGCCGTTCATGATGGTGATc < 1:334444/136‑1 (MQ=255) cAGCACTGGCTGGTCGTTGCGGAACTGGCTTTCCAGCAGTGCCTGCAGGTCGCTGTAGAAGCGCTGCTTGGCCGGGAACTTGGTGGGGTGGTCGCGGCTTTCGCCTTGGGGGAAATAGCCGTTCATGATGGTGATc < 1:223497/136‑1 (MQ=255) cAGCACTGGCTGGTCGTTGCGGAACTGGCTTTCCAGCAGTGCCTGCAGGTCGCTGTAGAAGCGCTGCTTGGCCGGGAACTTGGTGGGGTGGTCGCGGCTTTCGCCTTGGGGGAAATAGCCGTTCATGATGGTGATc < 1:491631/136‑1 (MQ=255) ggTCGTTGCGGAACTGGCTTTCCAGCAGTGCCTGCAGGTCGCTGTAGAAGCGCTGCTTGGCCGGGAACTTGGTGGGGTGGTCGCGGGGTTGGGCGTGGGGGGAATAGCCGTTGATGATGGTGGTCGGGCTGCCggt > 1:301316/1‑134 (MQ=255) ggTCGTTGCGGAACTGGCTTTCCAGCAGTGCCTGCAGGTCGCTGTAGAAGCGCTGCTTGGCCGGGAACTTGGTGGGGTGGTCGCGGGGTTAGCCGTGGGGGGAATAGCCGGTGATGATGGTGATCGGGCTGGCggt > 1:291175/1‑134 (MQ=255) ttGCGGAACTGGCTTTCCAGCAGTGCCTGCAGGTCGCTGTAGAAGCGCTGCTTGGCCGGGAACTTGGTGGGGTGGTCGCGGGGTTAGCCGTGGGGGAAATAGCCGGTCATGTTGGTTATCGGGCTGGCCTTGGCaa > 1:277475/1‑135 (MQ=255) tGCGGAACTGGCTTTCCAGCAGTGCCTGCAGGTCGCTGTAGAAGCGCTGCTTGGCCGGGAACTTGGTGGGGTGGTCGCGGCTTTCGCCTTGGGGGAAATAGCCGTTCATGATGGTGATCGGGCTGCCGTTGGCATc < 1:116372/136‑1 (MQ=255) tGCGGAACTGGCTTTCCAGCAGTGCCTGCAGGTCGCTGTAGAAGCGCTGCTTGGCCGGGAACTTGGTGGGGTGGTCGCGGCTTTCGCCTTGGGGGAAATAGCCGTTCATGATGGTGATCGGGCTGCCGTTGGCATc < 1:405890/136‑1 (MQ=255) gAACTGGCTTTCCAGCAGTGCCTGCAGGTAGCTGTAGAAGCGCTGCTTGGCCGGGAACTTGGTGGGGTGGTCGCGGCTTTCGCCTTGGGGGAAATAGCCGTTCATGATGGTGATCGGGCTGCCGTTGGCATCGGCa > 2:373905/1‑136 (MQ=255) gAACTGGCTTTCCAGCAGTGCCTGCAGGTAGCTGTAGAAGCGCTGCTTGGCCGGGAACTTGGTGGGGTGGTCGCGGCTTTCGCCTTGGGGGAAATAGCCGTTCATGATGGTGATCGGGCTGCCGTTGGCATCGGCa > 2:376714/1‑136 (MQ=255) gcagTGCCTGCAGGTCGCTGTAGAAGCGCTGCTTGGCCGGGAACTTGGTGGGGTGGTCGCGGCTTTGGCCGTGGGGGAAATAGCCGTTGATGATGGTGATAGGGCTGGCGGTGGCATAGGCAAAGGTAACCCAGAt > 2:292366/1‑136 (MQ=255) ccTGCAGGTAGCTGTAGAAGCGCTGCTTGGCCGGGAACTTGGTGGGGTGGTCGCGGCTTTCGCCTTGGGGGAAATAGCCGTTCATGATGGTGATCGGGCTGCCGTTGGCATCGGCAAAGGTACCCCAGATGAAgcg < 1:376714/136‑1 (MQ=255) ccTGCAGGTAGCTGTAGAAGCGCTGCTTGGCCGGGAACTTGGTGGGGTGGTCGCGGCTTTCGCCTTGGGGGAAATAGCCGTTCATGATGGTGATCGGGCTGCCGTTGGCATCGGCAAAGGTACCCCAGATGAAgcg < 1:373905/136‑1 (MQ=255) gCAGGTCGCTGTAGAAGCGCTGCTTGGCCGGGAACTTGGTGGGGTGGTCGCGGCTTTCGCCTTGGGGGAAATAGCCGGTGATGATGGTGATCGGGCTGCCGTTGGGATCGGCAAAGGAACCCCAGATGTAgcggcg > 1:104462/1‑136 (MQ=255) gTCGCTGTAGAAGCGCTGCTTGGCCGGGAACTTGGTGGGGTGGTCGCGGCTTTCGCCTTGGGGGAAATAGCCGTTCATGATGGTGATCGGGCTGCCGTTGGCATCGGCAAAGGTACCCCAGATGAAGCGGCGCTgg < 1:300720/136‑1 (MQ=255) gTCGCTGTAGAAGCGCTGCTTGGCCGGGAACTTGGTGGGGTGGGCGCGGCTTTCGCCTTGGGGGAAATAGCCGTTCATGATGGTGATCGGGCTGCCGTTGGCATCGGCAAAGGTACCCCAGATGAAGCGGCGCTgg < 1:446215/136‑1 (MQ=255) | TCAGCCAGCGTTTGGCGTTGTCCGGGCCAATGCCGATATCACAGTCTTGCGGCGAGATGTTCATGTCGCCCATCACCAGCACTGGCTGGTCGTTGCGGAACTGGCTTTCCAGCAGTGCCTGCAGGTCGCTGTAGAAGCGCTGCTTGGCCGGGAACTTGGTGGGGTGGTCGCGGCTTTCGCCTTGGGGGAAATAGCCGTTCATGATGGTGATCGGGCTGCCGTTGGCATCGGCAAAGGTACCCCAGATGAAGCGGCGCTGG > NC_002947/3292585‑3292844 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |