Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,376,667	C→A	23.5%	G34W (GGG→TGG)	PP_2968 ←	high‑affinity nickel/cobalt transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,376,667	0	C	A	23.5%	38.1 / 2.9	17	G34W (GGG→TGG)	PP_2968	high‑affinity nickel/cobalt transporter
Reads supporting (aligned to +/- strand):  ref base C (7/6);  new base A (2/2);  total (9/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.22e-01

ATGGCCACCAGCCAAACCACTGCGGTGTGGGAAAGCGTTGAGGCCAGTCCCAGCAGAACAGCCTGCTTCACCGTGCCGCGAATGGCCACGATAAACGCCGCCATCATGGTTTTCGAATGCCCTGGCTCTAGGCCATGCAAGGCTCCCAGCAGGATGGCACTGGGGAAGTACAACCAGGCCTGCGAGGCTCCCTGTTGCAGCAGTTCAGCGAAGCTCGACATGGGAAAAGGCCTAGAGGTACTTAGTGATTTGC  >  NC_002947/3376546‑3376798                                                                                                                          |                                                                                                                                    aTGGCCACCAGCCAAACCACTGCGGTGTGGGAAAGCGTTGAGGCCAGTCCCAGCAGAACAGCCTGCTTCACCGTGCCGCGAATGGCCACGATAAACGCCGCCATCATGGTTTTCGAATGCCCTGGCTCTAGGCCAt                                                                                                                       >  1:220872/1‑136 (MQ=255)     ccaccaGCCAAACCACTGCGGTGTGGGAAAGCGTTGAGGCCAGTCCCAGCAGAACAGCCTGCTTCACCGTGCCGCGAATGGCCACGATAAACGCCGCCATCATGGTTTTCGAATGCCATg                                                                                                                                   >  2:544233/1‑120 (MQ=255)     ccaccaGCCAAACCACTGCGGTGTGGGAAAGCGTTGAGGCCAGTCCCAGCAGAACAGCCTGCTTCACCGTGCCGCGAATGGCCACGATAAACGCCGCCATCATGGTTTTCGAATGCCATg                                                                                                                                   <  1:544233/120‑1 (MQ=255)               aaCCACTGCGGTGTGGGAAAGCGTTGAGGCCAGTCCCAGCAGAACAGCCTGCTTCACCGTGCCGCGAATGGCCACGATAAACGCCGCCATCATGGTTTTCGAATGCCCTGGCTCTAGGCCATGCAAGGCTCCcagc                                                                                                         >  2:394524/1‑136 (MQ=255)                aCCACTGCGGTGTGGGAAAGCGTTGAGGCCAGTCCCAGCAGAACAGCCTGCTTCACCGTGCCGCGAATGGCCACGATAAACGCCGCCATCATGGTTTTCGAATGCCCTGGCTCTAGGCCATGCAAGGCTCCcagca                                                                                                        <  1:264466/136‑1 (MQ=255)                   aCTGCGGTGTGGGAAAGCGTTGAGGCCAGTCCCAGCAGAACAGCCTGCTTCACCGTGCCGCGAATGGCCACGATAAACGCCGCCATCATGGTTTTCGAATGCCCTgg                                                                                                                                  <  1:141378/107‑1 (MQ=255)                   aCTGCGGTGTGGGAAAGCGTTGAGGCCAGTCCCAGCAGAACAGCCTGCTTCACCGTGCCGCGAATGGCCACGATAAACGCCGCCATCATGGTTTTCGAATGCCCTgg                                                                                                                                  >  2:141378/1‑107 (MQ=255)                                                 cccAGCAGAACAGCCTGCTTCACCGTGCCGCGAATGGCCACGATAAACGCCGCCATCATGGTTTTCGAATGCCCTGGCTCTAGGCCATGCAAGGCTCCCAGCAGGATGGCACTg                                                                                             <  2:341643/114‑1 (MQ=255)                                                 cccAGCAGAACAGCCTGCTTCACCGTGCCGCGAATGGCCACGATAAACGCCGCCATCATGGTTTTCGAATGCCCTGGCTCTAGGCCATGCAAGGCTCCCAGCAGGATGGCACTg                                                                                             >  1:341643/1‑114 (MQ=255)                                                                             cgcgAATGGCCACGATAAACGCCGCCATCATGGTTTTCGAATGCCCTGGCTCTAGGCCATGCAAGGCTCCCAGCAGGATGGCACTGGGGAAGTACAACCAGGCCTGCg                                                                       <  1:101884/108‑1 (MQ=255)                                                                             cgcgAATGGCCACGATAAACGCCGCCATCATGGTTTTCGAATGCCCTGGCTCTAGGCCATGCAAGGCTCCCAGCAGGATGGCACTGGGGAAGTACAACCAGGCCTGCg                                                                       >  2:101884/1‑108 (MQ=255)                                                                                         cGATAAACGCCGCCATCATGGTTTTCGAATGCCCTGGCTCTAGGCCATGCAAGGCTCCCAGCAGGATGGCACTGGGGAAGTACAACCAGGCCTGCGAGGCTCCCTGTTGCAGCAGTTCAGCGAAGCTCGACATggg                               >  1:346533/1‑136 (MQ=255)                                                                                         cGATAAACGCCGCCATCATGGTTTTCGAATGCCCTGGCTCTAGGCCATGCAAGGCTCCCAGCAGGATGGCACTGGGGAAGTACAACCAGGCCTGCGAGGCTCCCTGTTGCAGCAGTTCAGCGAAGCTCGACATggg                               <  1:238745/136‑1 (MQ=255)                                                                                                       atcatGGTTTTCGAATGCCCTGGCTCTAGGCCATGCAAGGCTCCCAGCAGGATGGCACTGGGGAAGTACAACCAGGCCTGCGAGGCTCCCTGTTGCAGCAGTTCAGCGAAGCTCGACATGGGAAAAGGCCTAGAgg                 >  1:116742/1‑136 (MQ=255)                                                                                                                    aaTGCCATGGCTCTAGGCCATGCAAGGCTCCCAGCAGGATGGCACTGGGGAAGTACAACCAGGCCTGCGAGGCTCCCTGTTGCAGCAGTTCAGCGAAGCTCGACATGGGAAAAGGCCTAGAGGTACTTAGTGAttt    >  1:336380/1‑136 (MQ=255)                                                                                                                      tGCCCTGGCTCTAGGCCATGCAAGGCTCCCAGCAGGATGGCACTGGGGAAGTACAACCAGGCCTGCGAGGCTCCCTGTTGCAGCAGTTCAGCGAAGCTCGACATGGGAAAAGGCCTAGAGGTACTTAGTGATTTGc  <  2:346533/136‑1 (MQ=255)                                                                                                                      tGCCATGGCTCTAGGCCATGCAAGGCTCCCAGCAGGATGGCACTGGGGAAGTACAACCAGGCCTGCGAGGCTCCCTGTTGCAGCAGTTCAGCGAAGCTCGACATGGGAAAAGGCCTAGAGGTACTTAGTGATTTGc  <  2:336380/136‑1 (MQ=255)                                                                                                                          |                                                                                                                                    ATGGCCACCAGCCAAACCACTGCGGTGTGGGAAAGCGTTGAGGCCAGTCCCAGCAGAACAGCCTGCTTCACCGTGCCGCGAATGGCCACGATAAACGCCGCCATCATGGTTTTCGAATGCCCTGGCTCTAGGCCATGCAAGGCTCCCAGCAGGATGGCACTGGGGAAGTACAACCAGGCCTGCGAGGCTCCCTGTTGCAGCAGTTCAGCGAAGCTCGACATGGGAAAAGGCCTAGAGGTACTTAGTGATTTGC  >  NC_002947/3376546‑3376798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑