| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,506,031 | G→T | 14.8% | intergenic (‑258/‑228) | PP_5566 ← / → PP_5567 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,506,031 | 0 | G | T | 14.8% | 81.5 / 2.0 | 27 | intergenic (‑258/‑228) | PP_5566/PP_5567 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (16/7); new base T (2/2); total (18/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01 | |||||||||||
TGTGACGGGCCATTCGGCGATTGCCGGGTCGCCTCCGGGCGCCAGTGAGGAAGATGAAGGATGGCGATATGACATTGCCTTGCAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGC > NC_002947/3505904‑3506161 | tgtgACGGGCCATTCGGCGATTGCCGGGTCGCCTCCGGGCGCCAGTGAGGAAGATGAAGGATGGCGATATGACATTGCCTTGCAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCtt > 1:252661/1‑136 (MQ=255)tgtgACGGGCCATTCGGCGATTGCCGGGTCGCCTCCGGGCGCCAGTGAGGAAGATGAAGGATGGCGATATGACATTGCCTTGCAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCtt > 1:338318/1‑136 (MQ=255) ggCCATTCGGCGATTGCCGGGTCGCCTCCGGGCGCCAGTGAGGAAGATGAAGGATGGCGATATGACATTGCCTTGCAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCgg < 2:338318/136‑1 (MQ=255) cATTCGGCGATTGCCGGGTCGCCTCCGGGCGCCAGTGAGGAAGATGAAGGATGGCGATATGACATTGCCTTGCAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACATGGCAGCTTTTGCCGgtgt > 2:47839/1‑136 (MQ=255) ttCGGCGATTGCCGGGTCGCCTCCGGGCGCCAGTGAGGAAGATGAAGGATGGCGATATGACATTGCCTTGCAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGttt < 1:39031/136‑1 (MQ=255) cGGGCGCCAGTGAGGAAGATGAAGGATGGCGATATGACATTGCCTTGCAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCctggct < 1:304721/136‑1 (MQ=255) tGAGGAAGATGAAGGATGGCGATATGACATTGCCTTGCAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTgg > 2:440701/1‑136 (MQ=255) gAAGGATGGCGATATGACATTGCCTTGCAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAAcc > 2:437859/1‑136 (MQ=255) gAAGGATGGCGATATGACATTGCCTTGCAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCATGGCTGGCATTCTGGAATGTGAAcc > 2:33194/1‑136 (MQ=255) aTGGCGATATGACATTGCCTTGCAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTc > 1:446560/1‑136 (MQ=255) cATTGCCTTGCAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCa < 2:447706/136‑1 (MQ=255) ccTTGCAGGGCGGTATTGCCCCGTTTTCCTATGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATTCa > 2:64269/1‑136 (MQ=255) cTTGCAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCat > 1:508405/1‑136 (MQ=255) ggCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCa > 1:215422/1‑136 (MQ=255) tATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCActtta > 1:409087/1‑135 (MQ=255) ccccGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCActttcctttc > 2:536054/1‑136 (MQ=255) tCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCGTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTgcaggca > 1:503836/1‑136 (MQ=255) tCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTAGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTgcaggca > 1:545251/1‑136 (MQ=255) cTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGt < 2:215422/136‑1 (MQ=255) gCCTGTAGTGAGTTGACATGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCa < 1:47839/136‑1 (MQ=255) tAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTACTTTCTGCAGGCAGTCGCATGttt > 2:327148/1‑136 (MQ=255) tGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCg > 1:294346/1‑136 (MQ=255) gTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCaa < 1:437859/136‑1 (MQ=255) gTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCaa < 1:33194/136‑1 (MQ=255) tGACATGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCA‑TTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGc > 1:87871/1‑135 (MQ=255) tGACATGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCA‑TTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGc < 2:87871/135‑1 (MQ=255) tGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCACGCAGTCGCATGTTTCCGCAAGc > 1:333367/1‑136 (MQ=255) | TGTGACGGGCCATTCGGCGATTGCCGGGTCGCCTCCGGGCGCCAGTGAGGAAGATGAAGGATGGCGATATGACATTGCCTTGCAGGGCGGTATTGCCCCGTTTTCCTAGGCCTGTAGTGAGTTGACAGGGCAGCTTTTGCCGGTGTTTCCTTTGGGCCTTTCTGCCTGGCTGGCATTCTGGAATGTGAACCCTGTCGCATTTATTTCAATGCATGATGGCACTTTCCTTTCTGCAGGCAGTCGCATGTTTCCGCAAGC > NC_002947/3505904‑3506161 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |