| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,864,440 | G→T | 24.9% | R94L (CGC→CTC) | cobM → | precorrin‑4 C(11)‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,864,440 | 0 | G | T | 24.9% | 37.0 / 3.1 | 16 | R94L (CGC→CTC) | cobM | precorrin‑4 C(11)‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (9/3); new base T (2/2); total (11/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
TCAACAGCGCCGAACTGCACCTGGAACAGATCATCGCCGCCATGCGCAGCGCACATGAAAAAGGTCAGGATGTGGCCCGGGTGCACAGCGGCGACCCCAGCCTGTACGGCGCCATCGGTGAGCAGATTCGCCACCTGCAAGCGCTGGGCATCGATTACCAGATCATCCCCGGCGTTACCGCCACGGCAGCGAGCGCCGCCCTGCTCGGCTGCGAGCTGACGTTGCCTCAAGTGGCGCAGACCGTGATCCTCACCCGCTATG > NC_002947/3864311‑3864571 | tCAACAGCGCCGAACTGCACCTGGAACAGATCATCGCCGCCATGCGCAGCGCACATGAAAAAGGTCAGGATGTGGCCCGGGTGCACAGCGGCGACCCCAGCCTGTACGGCGCCATCGGTGAGCAGATTCTCCACCt > 1:284337/1‑136 (MQ=255)tCAACAGCGCCGAACTGCACCTGGAACAGATCATCGCCGCCATGCGCAGCGCACATGAAAAAGGTCAGGATGTGGCCCGGGTGCACAGCGGCGACCCCAGCCTGTACGGCGCCATCGGTGAGCAGATTCTCCACCt > 1:287046/1‑136 (MQ=255) cAGCGCCGAACTGCACCTGGAACAGATCATCGCCGCCATGCGCAGCGCACATGAAAAAGGTCAGGATGTGGCCCGGGTGCACAGCGGCGACCCCAGCCTGTACGGCGCCATCGGTGAGCAGATTCGCCACCTGCaa < 1:469258/136‑1 (MQ=255) gAACTGCACCTGGAACAGATCATCGCCGCCATGCGCAGCGCACATGAAAAAGGTCAGGATGTGGCCCGGGTGCACAGCGGCGACCCCAGCCTGTACGGCGCCATCGGTGAGCAGATTCGCCACCTGCAAGCGCTgg < 1:84575/136‑1 (MQ=255) tGCACCTGGAACAGATCATCGCCGCCATGCGCAGCGCACATGAAAAAGGTCAGGATGTGGCCCGGGTGCACAGCGGCGACCCCAGCCTGTACGGCGCCATCGGTGAGCAGATTCGCCACCTGCAAGCGCTGGGCAt > 1:427788/1‑136 (MQ=255) cTGGAACAGATCATCGCCGCCATGCGCAGCGCACATGAAAAAGGTCAGGATGTGGCCCGGGTGCACAGCGGCGACCCCAGCCTGTACGGCGCCATCGGTGAGCAGATTCGCCACCTGCAAGCGCTGGGCATCGAtt > 2:497075/1‑136 (MQ=255) aTGAAAAAGGTCAGGATGTGGCCCGGGTGCACAGCGGCGACCCCAGCCTGTACGGCGCCATCGGTGAGCAGATTCGCCACCTGCAAGCGCTGGGCATCGATTACCAGATCATCCCCGGCGTTACCGCCACGGCagc > 2:104074/1‑136 (MQ=255) tgTCAGGATGTGGCCCGGGTGCACAGCGGCGACCCCAGCCTGTACGGCGCCATCGGTGAGCAGATTCGCCACCTGCAAGCGCTGTGCATCGATTACCAGATCATCCCCGGCGTTACCGCCACGGCAGCGAGcgccc > 2:344108/2‑135 (MQ=255) aTGTGGCCCGGGTGCACAGCGGCGACCCCAGCCTGTACGGCGCCATCGGTGAGCAGATTCGCCACCTGCAAGCGCTGGGCATCGATTACCAGATCATCCCCGGCGTTACCGCCACGGCAGCGAGCGCCGCCCTGCt > 1:447778/1‑136 (MQ=255) gtgGCCCGGGTGCACAGCGGCGACCCCAGCCTGTACGGCGCCATCGGTGAGCAGATTCGCCACCTGCAAGCGCTGGGCATCGATTACCAGATCATCCCCGGCGTTACCGCCACGGCAGCGAGCGCCGCCCTGCTCg > 1:439285/1‑136 (MQ=255) gtgGCCCGGGTGCACAGCGGCGACCCCAGCCTGTACGGCGCCATCGGTGAGCAGATTCGCCACCTGCAAGCGCTGGGCATCGATTACCAGATCATCCCCGGCGTTACCGCCACGGCAGCGAGCGCCGCCCTGCTCg > 1:49938/1‑136 (MQ=255) aGCGGCGACCCCAGCCTGTACGGCGCCATCGGTGAGCAGATTCTCCACCTGCAAGCGCTGGGCATCTATTACCAGATCATCCCCGGCGTTACCGCCACTGCAGCGAGCGCCGCCCTGCTCGGCTGCGAGCTGACGt < 2:284337/136‑1 (MQ=255) aGCGGCGACCCCAGCCTGTACGGCGCCATCGGTGAGCAGATTCTCCACCTGCAAGCGCTGGGCATCTATTACCAGATCATCCCCGGCGTTACCGCCACTGCAGCGAGCGCCGCCCTGCTCGGCTGCGAGCTGACGt < 2:287046/136‑1 (MQ=255) cgACCCCAGCCTGTACGGCGCCATCGGTGAGCAGATTCGCCACCTGCAAGCGCTGGGCATCGATTACCAGATCATCCCCGGCGTTACCGCCACGGCAGCGAGCGCCGCCCTGCTCGGCTGCGAGCTGACGTTGCCt > 2:198754/1‑136 (MQ=255) cAGCCTGTACGGCGCCATCGGTGAGCAGATTCGCCACCTGCAAGCGCTGGGCATCGATTACCAGATCATCCCCGGCGTTACCGCCACGGCAGCGAGCGCCGCCCTGCTCGGCTGCGAGCTGACGTTGCCTCAAGTg < 1:104074/136‑1 (MQ=255) aTTCGCCACCTGCAAGCGCTGGGCATCGATTACCAGATCATCCCCGGCGTTACCGCCACGGCAGCGAGCGCCGCCCTGCTCGGCTGCGAGCTGACGTTGCCTCAAGTGGCGCAGACCGTGATCCTCACCCGCTATg > 1:306849/1‑136 (MQ=255) | TCAACAGCGCCGAACTGCACCTGGAACAGATCATCGCCGCCATGCGCAGCGCACATGAAAAAGGTCAGGATGTGGCCCGGGTGCACAGCGGCGACCCCAGCCTGTACGGCGCCATCGGTGAGCAGATTCGCCACCTGCAAGCGCTGGGCATCGATTACCAGATCATCCCCGGCGTTACCGCCACGGCAGCGAGCGCCGCCCTGCTCGGCTGCGAGCTGACGTTGCCTCAAGTGGCGCAGACCGTGATCCTCACCCGCTATG > NC_002947/3864311‑3864571 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |