| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,936,492 | G→T | 26.7% | A93A (GCC→GCA) | PP_5604 ← | subtilisin‑like serine protease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,936,492 | 0 | G | T | 26.7% | 29.6 / 2.9 | 15 | A93A (GCC→GCA) | PP_5604 | subtilisin‑like serine protease |
| Reads supporting (aligned to +/- strand): ref base G (5/6); new base T (2/2); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
GCCCACCCTGCGCCGGGCTGGATGCACACAACAGCACACCTGCGGCCAAAGCCTCGGCACAGGCCTGGTGCAGCACGGGCCGGTCCTGCTGCAAGCCCAGGCTGAGGTTGACCAGCGTGGCTCCCGACTCCACCAGCCACAGCAATGCGGCAGCCACCTGCAACGCACTGGTGCTGGCCTGGGCACTGAACACCTGGGCCACCAGCACCGACACAGGACCGGCCTGGTGTTGCAAGCTGGCGAGCACCG > NC_002947/3936374‑3936622 | gCCCACCCTGCGCCAGGCTGGATGCACACAACAGCACACCTGCGGCCAAAGCCTCGGCACAGGCCTGGTGCAGCACGGGCCGGTCCTGCTGCAAGCCCAGGCTGAGGTTGACCAGCGTGGCTCCCGACTCCACCAg < 2:418976/136‑1 (MQ=255) tGGATGCACACAACAGCACACCTGCGGCCAAAGCCTCGGCACAGGCCTGGTGCAGCACGGGCCGGTCCTGCTGCAAGCCCAGGCTGAGGTTGACCAGCGTTGCTCCCGACTCCACCAGCCACAGCAATGCGGCAGc > 1:10280/1‑136 (MQ=255) tGGATGCACACAACAGCACACCTGCGGCCAAAGCCTCGGCACAGGCCTGGTGCAGCACGGGCCGGTCCTGCTGCAAGCCCAGGCTGAGGTTGACCAGCGTTGCTCCCGACTCCACCAGCCACAGCAATGCGGCAGc > 1:45217/1‑136 (MQ=255) acCTGCGGCCAAAGCCTCGGCACAGGCCTGGTGCAGCACGGGCCGGTCCTGCTGCAAGCCCAGGCTGAGGTTGACCAGCGTGGCTCCCGACTCCACCAGCCACAGCAATGCGGCAGCCACCTGCAACGCACTGGTg > 1:200751/1‑136 (MQ=255) cGGCCAAAGCCTCGGCACAGGCCTGGTGCAGCACGGGCCGGTCCTGCTGCAAGCCCAGGCTGAGGTTGACCAGCGTGGCTCCCGACt < 2:128574/87‑1 (MQ=255) cGGCCAAAGCCTCGGCACAGGCCTGGTGCAGCACGGGCAGGTCCTGCTGCAAGCCCAGGCTGAGGTTGACCAGCGTGGCTCCCGACt > 1:128574/1‑87 (MQ=255) gcagcaCGGGCCGGTCCTGCTGCAAGCCCAGGCTGAGGTTGACCAGCGTGGCTCCCGACTCCACCAGCCACAGCAATGCGGCAGCCACCTGCAACGCACTGGTGCTGGCCTGGGCACTGAACACCTGGGCCACCAg < 1:90070/136‑1 (MQ=255) ccagcaCGGGCCGGTCCTGCTGCAAGCCCAGGCTGAGGTTGACCAGCGTGGCTCCCGACTCCACCAGCCACAGCAATGCGGCAGCCACCTGCAACGCACTGGTGCTGGCCTGGGCACTGAACACCTGGGCCACCAg < 1:417739/135‑1 (MQ=255) cagcaCGGGCCGGTCCTGCTGCAAGCCCAGGCTGAGGTTGACCAGCGTGGCTCCCGACTCCACCAGCCACAGCAATGCGGCAGCCACCTGCAACGCACTGGTGCTGGCCTGGGCACTGAACACCTGGGCCACCAGc > 2:254503/1‑136 (MQ=255) ccTGCTGCAAGCCCAGGCTGAGGTTGACCAGCGTTGCTCCCGACTCCACCAGCCACAGCAATGCGGCAGCCACCTGCAACGCACTGGTGCTGGCCTGGGCACTGAACACCTGGGCCACCAGCACCGACACAGGAcc < 2:10280/136‑1 (MQ=255) ccTGCTGCAAGCCCAGGCTGAGGTTGACCAGCGTTGCTCCCGACTCCACCAGCCACAGCAATGCGGCAGCCACCTGCAACGCACTGGTGCTGGCCTGGGCACTGAACACCTGGGCCACCAGCACCGACACAGGAcc < 2:45217/136‑1 (MQ=255) ctgctgCAAGCCCAGGCTGAGGTTGACCAGCGTGGCTCCCGACTCCACCAGCCACAGCAATGCGGCAGCCACCTGCAACGCACTGGTGCTGGCCTGGGCACTGAACACCTGGGCCACCAGCACCGACACAGGACCg < 2:200751/136‑1 (MQ=255) cAGGCTGAGGTTGACCAGCGTGGCTCCCGACTCCACCAGCCACAGCAATGCGGCAGCCACCTGCAACGCACTGGTGCTGGCCTGGGCACTGAACACCTGGGCCACCAGCACCGACACAGGACCGGCCTGGTGTTGc < 1:83300/136‑1 (MQ=255) cTGAGGTTGACCAGCGTGGCTCCCGACTCCACCAGCCACAGCAATGCGGCAGCCACCTGCAACGCACTGGTGCTGGCCTGGGCTCTGAACACCTGGGCCACCAGCACCGACACAGGACCGGCCTGGTGTTGCAAGc > 2:365552/1‑136 (MQ=255) aGCGTGGCTCCCGACTCCACCAGCCACAGCAATGCGGCAGCCACCTGCAACGCACTGGTGCTGGCCTGGGCACTGAACACCTGGGCCACCAGCACCGACACAGGACCGGCCTGGTGTTGCAAGCTGGCGAGCAccc > 1:79459/1‑135 (MQ=255) | GCCCACCCTGCGCCGGGCTGGATGCACACAACAGCACACCTGCGGCCAAAGCCTCGGCACAGGCCTGGTGCAGCACGGGCCGGTCCTGCTGCAAGCCCAGGCTGAGGTTGACCAGCGTGGCTCCCGACTCCACCAGCCACAGCAATGCGGCAGCCACCTGCAACGCACTGGTGCTGGCCTGGGCACTGAACACCTGGGCCACCAGCACCGACACAGGACCGGCCTGGTGTTGCAAGCTGGCGAGCACCG > NC_002947/3936374‑3936622 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |