| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,953,465 | G→T | 19.0% | P56P (CCC→CCA) | PP_3484 ← | two‑component system response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,953,465 | 0 | G | T | 19.0% | 55.3 / 2.4 | 21 | P56P (CCC→CCA) | PP_3484 | two‑component system response regulator |
| Reads supporting (aligned to +/- strand): ref base G (8/9); new base T (2/2); total (10/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01 | |||||||||||
CTTGAACAGGACGTGACTCACTCCGAGTTCGGCGGCCCGCTCACGCACCTCGACGGTGGGGTGGGCGGTGATCAGCACGAAGTGGCATTGCCTGTTCTTGCGTACAGCCTCCAGAACCTGAAAACCTTCCATGTCGGGCAGGCGGTAATCCAGCACAACTACTTGCGGTGCCAGGCTTTCAGCCTGTTCGATACCACTGGCACCGTCGTGGGCAACATGAACTTCCAGGCCTTGCGCCTGCAGATACGCTTGCAGGTTTTGCGCAAG > NC_002947/3953330‑3953596 | cTTGAACAGGACGTGACTCACTCCGAGTTCGGCGGCCCGCTCACGCACCTCGACGGTGGGGTGGGCGGTGATCAGCACGAAGTGGCATTGCCTGTTCTTGCGTACAGCCTCCAGAACCTGAAAACCTTCCATGTCg < 1:21045/136‑1 (MQ=255) ccTCGACGGTGGGGTGGGCGGTGATCAGCACGAAGTGGCATTGCCTGTTCTTGCGTACAGCCTCCAGAACCTGAAAACCTTCCATGTCGGGCAGGCGGTAATCCAGCACAACTACTTGCGGTGCCAGGCTTTCAGc < 1:151290/136‑1 (MQ=255) ccTCGACGGTGGGGTGGGCGGTGATCAGCACGAAGTGGCATTGCCTGTTCTTGCGTACAGCCTCCAGAACCTGAAAACCTTCCATGTCGGGCAGGCGGTAATCCAGCACAACTACTTGCGGTGCCAGGCTTTCAGc < 1:324940/136‑1 (MQ=255) ccTCGACGGTGGGGTGGGCGGTGATCAGCACGAAGTGGCATTGCCTGTTCTTGCGTACAGCCTCCAGAACCTGAAAACCTTCCATGTCGGGCAGGCGGTAATCCAGCACAACTACTTGCGGTGCCAGGCTTTCAGc < 1:393754/136‑1 (MQ=255) cGGTGGGGTGGGCGGTGATCAGCACGAAGTGGCATTGCCTGTTCTTGCGTACAGCCTCCAGAACCTGAAAACCTTCCATGTCGGGCAGGCGGTAATCCAGCACAACTACTTGCGGTGCCAGGCTTTCAGCCTGTTc > 1:139153/1‑136 (MQ=255) tgggCGGTGATCAGCACGAAGTGGCATTGCCTGTTCTTGCGTACAGCCTCCAGAACCTGAAAACCTTCCATGTCGGGCAGGCGGTAATCCAGCACAACTACTTGCGGTGCCAGGCTTTCAGCCTGTTCGATACCAc > 2:48924/1‑136 (MQ=255) tgggCGGTGATCAGCACGAAGTGGCATTGCCTGTTCTTGCGTACAGCCTCCAGAACCTGAAAACCTTCCATGTCGGGCAGGCGGTAATCCAGCACAACTACTTGCGGTGCCAGGCTTTCAGCCTGTTCGATACCAc > 1:444580/1‑136 (MQ=255) tgggCGGTGATCAGCACGAAGTGGCATTGCCTGTTCTTGCGTACAGCCTCCAGAACCTGAAAACCTTCCATGTCGGGCAGGCGGTAATCCAGCACAACTACTTGCGGTGCCAGCCTTTCAGCCTGTTCGATACCAc > 2:459067/1‑136 (MQ=255) ggCGGTGATCAGCACGAAGTGGCATTGCCTGTTCTTGCGTACAGCCTCCAGAACCTGAAAACCTTCCATGTCGGGCAGGCGGTAATCCAGCACAACTACTTGCGGTGCCAGGCTTTCAGCCTGTTCGATACCACTg < 2:444580/136‑1 (MQ=255) tCAGCACGAAGTGGCATTGCCTGTTCTTGCGTACAGCCTCCAGAACCTGAAAACCTTCCATGTCGGGCAGGCGGTAATCCAGCACAACTACTTGCGGTGCCAGGCTTTCAGCCTGTTCGATACCACTGGCACcgtc > 1:52692/1‑136 (MQ=255) aGCACGAAGTGGCATTGCCTGTTCTTGCGTACAGCCTCCAGAACCTGAAAACCTTCCATGTCGGGCAGGCGGTAATCCAGCACAACTACTTGCGGTGCCAGGCTTTCAGCCTGTTCGATACCACTGGCACcgtcgt < 1:48924/136‑1 (MQ=255) ggCATTGCCTGTTCTTGCGTACAGCCTCCAGAACCTGAAAACCTTCCATGTCGGGCAGGCGGTAATCCAGCACAACTACTTGCGGTGCCAGGCTTTCAGCCTGTTCGATACCACTGGCACCGTCGTGGGCAACATg > 1:173664/1‑136 (MQ=255) ggCATTGCCTGTTCTTGCGTACAGCCTCCAGAACCTGAAAACCTTCCATGTCGGGCAGGCGGTAATCCAGCACAACTACTTGCGGTGCCAGGCTTTCAGCCTGTTCGATACCACTGGCACCGTCGTGGGCAACATg > 2:257717/1‑136 (MQ=255) ccTGTTCTTGCGTACAGCCTCCAGAACCTGAAAACCTTCCATGTCGGGCAGGCGGTAATCCAGCACAACTACTTGCGGTGCCAGGCTTTCAGCCTGTTCGATACCACTGGCACCGTCGTGGGCAACATGAACTTcc < 1:148404/136‑1 (MQ=255) gTTCTTGCGTACAGCCTCCAGAACCTGAAAACCTTCCATGTCGGGCATGCGGTAATCCAGCACAACTACTTGCGGTGCCAGGCTTTCAGCCTGTTCGATACCACTGGCACCGTCGTGGGCAACATGAACTTCCAgg > 2:326554/1‑136 (MQ=255) tGCGTACAGCCTCCAGAACCTGAAAACCTTCCATGTCGGGCAGGCGGTAATCCAGCACAACTACTTGCGGTGCCAGGCTTTCAGCCTGTTCGATACCACTGGCACCGTCGTGGGCAACATGAACTTCCAGGCCTTg < 1:95109/136‑1 (MQ=255) tACAGCCTCCAGAACCTGAAAACCTTCCATGTCTGGCAGGCGGTAATCCAGCACAACTACTTGCGGTGCCAGGCTTTCAGCCTGTTCGATACCACTGGCACCGTCGTGGGCAACATGAACTTCCAGGCCTTGCGcc > 2:404198/1‑136 (MQ=255) tACAGCCTCCAGAACCTGAAAACCTTCCATGTCTGGCAGGCGGTAATCCAGCACAACTACTTGCGGTGCCAGGCTTTCAGCCTGTTCGATACCACTGGCACCGTCGTGGGCAACATGAACTTCCAGGCCTTGCGcc > 2:502393/1‑136 (MQ=255) caaCCTGAAAACCTTCCATGTCGGGCAGGCGGTAATCCAGCACAACTACTTGCGGTGCCAGGCTTTCAGCCTGTTCGATACCACTGGCACCGTCGTGGGCAACATGAACTTCCAGGCCTTGCGCCTGCAGATACGc < 2:524544/135‑1 (MQ=255) aaCCTGAAAACCTTCCATGTCGGGCAGGCGGTAATCCAGCACAACTACTTGCGGTGCCAGGCTTTCAGCCTGTTCGATACCACTGGCACCGTCGTGGGCAACATGAACTTCCAGGCCTTGCGCCTGCAGATACGCt < 2:480190/136‑1 (MQ=255) tGTCTGGCAGGCGGTAATCCAGCACAACTACTTGCGGTGCCAGGCTTTCAGCCTGTTCGATACCACTGGCACCGTCGTGGGCAACATGAACTTCCAGGCCTTGCGCCTGCAGATACGCTTGCAGGTTTTGCGCAag < 1:502393/136‑1 (MQ=255) tGTCTGGCAGGCGGTAATCCAGCACAACTACTTGCGGTGCCAGGCTTTCAGCCTGTTCGATACCACTGGCACCGTCGTGGGCAACATGAACTTCCAGGCCTTGCGCCTGCAGATACGCTTGCAGGTTTTGCGCAag < 1:404198/136‑1 (MQ=255) | CTTGAACAGGACGTGACTCACTCCGAGTTCGGCGGCCCGCTCACGCACCTCGACGGTGGGGTGGGCGGTGATCAGCACGAAGTGGCATTGCCTGTTCTTGCGTACAGCCTCCAGAACCTGAAAACCTTCCATGTCGGGCAGGCGGTAATCCAGCACAACTACTTGCGGTGCCAGGCTTTCAGCCTGTTCGATACCACTGGCACCGTCGTGGGCAACATGAACTTCCAGGCCTTGCGCCTGCAGATACGCTTGCAGGTTTTGCGCAAG > NC_002947/3953330‑3953596 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |