Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,125,189 C→A 18.1% P204P (CCC→CCA)  adhB → alcohol dehydrogenase cytochrome c subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,125,1890CA18.1% 95.3 / 6.4 33P204P (CCC→CCA) adhBalcohol dehydrogenase cytochrome c subunit
Reads supporting (aligned to +/- strand):  ref base C (17/10);  new base A (2/4);  total (19/14)
Fisher's exact test for biased strand distribution p-value = 3.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.17e-01

CGACATCCCCTGGCCGCTGAACCTGCGCTGGCCCATCGCCTTGTGGAACGGCCTGTTCGCCGCCACTACGCCTTACACCGACAAGGCCGGGCAGGATGCACAGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCCGGACACTGCGGCAGTTGCCATACGCCACGTGGCCTGGCCTTCAATGAAAAGGCGCTGGATGACAGCGGCAAGCCGTTCCTGTCCGGCGCACTGCTCGACGGCTGGTATGCACCCAGCCTACGCGCCGATC  >  NC_002947/4125054‑4125319
                                                                                                                                       |                                                                                                                                  
cGACATCCCCTGGCCGCTGAACCTGCGCTGGCCCATCGCCTTGTGGAACGGCCTGTTCGCCGCCACTACGCCTTACACCGACAAGGCCGGGCAGGATGCACAGTGGAACCGTGGCGCCTATATCGTCCAGGGcccc                                                                                                                                    <  1:406248/136‑1 (MQ=255)
   cATCCCCTGGCCGCTGAACCTGCGCTGGCCCATCGCCTTGTGGAACGGCCTGTTCGCCGCCACTACGCCTTACACCGACAAGGCCGGGCAGGATGCACAGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCCGGa                                                                                                                                 >  1:445453/1‑136 (MQ=255)
        ccTGGCCGCTGAACCTGCGCTGGCCCATCGCCTTGTGGAACGGCCTGTTCGCCGCCACTCCGCCTAACACCGACAAGGCCGGGCAGGATGCACAGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCCGGACACTg                                                                                                                            >  1:165710/1‑136 (MQ=255)
        ccTGGCCGCTGAACCTGCGCTGGCCCATCGCCTTGTGGAACGGCCTGTTCGCCGCCACTACGCCTTACACCGACAAGGCCGGGCAGGATGCACAGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCCGGACACTg                                                                                                                            >  2:23008/1‑136 (MQ=255)
           ggCCGCTGAACCTGCGCTGGCCCATCGCCTTGTGGAACGGCCTGTTCGCCGCCACTACGCCTTACACCGACAAGGCCGGGCAGGATGCACAGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCCGGACACTGCgg                                                                                                                         <  2:209718/136‑1 (MQ=255)
                         cgcTGGCCCATCGCCTTGTGGAACGGCCTGTTCGCCGCCACTACGCCTTACACCGACAAGGCCGGGCAGGATGCACAGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCCGGACACTGCGGCAGTTGCCATACGc                                                                                                           <  1:310557/136‑1 (MQ=255)
                         cgcTGGCCCATCGCCTTGTGGAACGGCCTGTTCGCCGCCACTACGCCTTACACCGACAAGGCCGGGCAGGATGCACAGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCCGGACACTGCGGCAGTTGCCATACGc                                                                                                           >  2:15373/1‑136 (MQ=255)
                          gcTGGCCCATCGCCTTGTGGAACGGCCTGTTCGCCGCCACTACGCCTTACACCGACAAGGCCGGGCAGGATGCACAGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCCGGACACTGCGGCAGTTGCCATACGcc                                                                                                          <  1:180278/136‑1 (MQ=255)
                            tGGCCCATCGCCTTGTGGAACGGCCTGTTCGCCGCCACTACGCCTTACACCGACAAGGCCGGGCAGGATGCACAGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCCGGACACTGCGGCAGTTGCCATACGCCAc                                                                                                        >  2:509976/1‑136 (MQ=255)
                                    cGCCTTGTGGAACGGCCTGTTCGCCGCCACTACGCCTTACACCGACAAGGCCGGGCAGGATGCACAGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCCGGACACTGCGGCAGTTGCCATACGCCACGtggcctg                                                                                                <  1:127638/136‑1 (MQ=255)
                                      ccTTGTGGAACGGCCTGTTCGCCGCCACTACGCCTTACACCGACAAGGCCGGGCAGGATGCACAGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCCGGACACTGCGGCAGTTGCCATACGCCACGtggcctggc                                                                                              >  1:514756/1‑136 (MQ=255)
                                                   ccTGTTCGCCGCCACTACGCCTTACACCGACAAGGCCGGGCAGGATGCACAGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCAGGACACTGCGGCAGTTGCCATACGCCACGTGGCCTGGCCTTCAATGAAAAg                                                                                 >  1:334609/1‑136 (MQ=255)
                                                          gccgccACTACGCCTTACACCGACAAGGCCGGGCAGGATGCACAGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCCGGACACTGCGGCAGTTGCCATACGCCACGTGGCCTGGCCTTCAATGAAAAGGCGCTgg                                                                          >  1:415118/1‑136 (MQ=255)
                                                                       cTTACACCGACAAGGCCGGGCAGGATGCACAGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCCGGACACTGCGGCAGTTGCCATACGCCACGTGGCCTGGCCTTCAATGAAAAGGCGCTGGATGACAGCGGCaa                                                             >  2:196617/1‑136 (MQ=255)
                                                                                 cAAGGCCGGGCAGGATGCACAGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCCGGACACTGCTGCAGTTGCCATACGCCACGTGGCCTGGCCTTCAATGAAAAGGCGCTGGATGACAGCGGCAAGCCGTTCCTg                                                   >  2:37597/1‑136 (MQ=255)
                                                                                       cGGGCAGGATGCACAGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCAGGACACTGCGGCAGTTGCCATACGCCACGTGGCCTGGCCTTCAATGAAAAGGCGCTGGATGACAGCGGCAAGCCGTTCCTGTCCGgc                                             <  2:351471/136‑1 (MQ=255)
                                                                                       cGGGCAGGATGCACAGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCAGGACACTGCGGCAGTTGCCATACGCCACGTGGCCTGGCCTTCAATGAAAAGGCGCTGGATGACAGCGGCAAGCCGTTCCTGTCCGgc                                             <  2:334609/136‑1 (MQ=255)
                                                                                       cGGGCAGGATGCACAGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCAGGACACTGCGGCAGTTGCCATACGCCACGTGGCCTGGCCTTCAATGAAAAGGCGCTGGATGACAGCGGCAAGCCGTTCCTGTCCGgc                                             <  2:100438/136‑1 (MQ=255)
                                                                                       cGGGCAGGATGCACAGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCAGGACACTGCGGCAGTTGCCATACGCCACGTGGCCTGGCCTTCAATGAAAAGGCGCTGGATGACAGCGGCAAGCCGTTCCTGTCCGgc                                             <  2:145151/136‑1 (MQ=255)
                                                                                        gggCAGGATGCACAGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCCGGACACTGCGGCAGTTGCCATACGCCACGTGGCCTGGCCTTCAATGAAAAGGCGCTGGATGACAGCGGCAAGCCGTTCCTGTCCGgcg                                            <  1:489705/136‑1 (MQ=255)
                                                                                           cAGGATGCACAGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCCGGACACTGCGGCAGTTGCCATACGCCACGTGGCCTGGCCTTCAATGAAAAGGCGCTGGATGACAGCGGCAAGCCGTTCCTGTCCGGCGCAc                                         >  1:43144/1‑136 (MQ=255)
                                                                                           cAGGATGCACAGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCCGGACACTGCGGCAGTTGCCATACGCCACGTGGCCTGGCCTTCAATGAAAAGGCGCTGGATGACAGCGGCAAGCCGTTCCTGTCCGGCGCAc                                         >  2:501059/1‑136 (MQ=255)
                                                                                                  cacaGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCCGGACACTGCGGCAGTTGCCATACGCCACGTGGCCTGGCCTTCAATGAAAAGGCGCTGGATGACAGCGGCAAGCCGTTCCTGTCCGGCGCACTGCTCGa                                  <  2:107915/136‑1 (MQ=255)
                                                                                                    caGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCCGGACACTGCGGCAGTTGCCATACGCCACGTGGCCTGGCCTTCAATGAAAAGGCGCTGGATGACAGCGGCAAGCCGTTCCTGTCCGGCGCACTGCTCGACg                                <  1:430099/136‑1 (MQ=255)
                                                                                                    caGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCCGGACACTGCGGCAGTTGCCATACGCCACGTGGCCTGGCCTTCAATGAAAAGGCGCTGGATGACAGCGGCAAGCCGTTCCTGTCCGGCGCACTGCTCGACg                                <  1:509976/136‑1 (MQ=255)
                                                                                                           aCCGTGGCGCCTATATCGTCCAGGGCCCCGGACACTGCGGCAGTTGCCATACGCCACGTGGCCTGGCCTTCAATGAAAAGGCGCTGGATGACAGCGGCAAGCCGTTCCTGTCCGGCGCCCTGCTCGACGGCTGGTa                         >  2:357160/1‑136 (MQ=255)
                                                                                                               tGGCGCCTATATCGTCCAGGGCCCCGGACACTGCGGCAGTTGCCATACGCCACGTGGCCTGGCCTTCAATGAAAAGGCGCTGGATGACAGCGGCAAGCCGTTCCTGTCCGGCGCACTGCTCGACGGCTGGTATGCa                     >  1:200404/1‑136 (MQ=255)
                                                                                                                  cgcCTATATCGTCCAGGGCCCCGGACACTGCGGCAGTTGCCATACGCCACGTGGCCTGGCCTTCAATGAAAAGGCGCTGGATGACAGCGGCAAGCCGTTCCTGTCCGGCGCACTGCTCGACGGCTGGTATGCAccc                  >  2:97993/1‑136 (MQ=255)
                                                                                                                  cgcCTATATCGTCCAGGGCCCCGGACACTGCGGCAGTTGCCATACGCCACGTGGCCTGGCCTTCAATGAAAAGGCGCTGGATGACAGCGGCAAGCCGTTCCTGTCCGGCGCACTGCTCGACGGCTGGTATGCAccc                  >  1:317322/1‑136 (MQ=255)
                                                                                                                  cgcCTATATCGACCAAGGCCCCGGACAATGCGGCAGTTGACATACGCCACGTGGCCTGGCCTTCAATGAAAAGACGCAGGATGACAGCGGCAAGCCATTCCTGTCCGGCTCACACCTCTACGCCTTGTATGCAccc                  >  2:64790/1‑136 (MQ=255)
                                                                                                                    ccTATATCGTCCAGGGCCCCGGACACTGCGGCAGTTGCCATACGCCACGTGGCCTGGCCTTCAATGAAAAGGCGCTGGATGACAGCGGCAAGCCGTTCCTGTCCGGCGCACTGCTCGACGGCTGGTATGCCCCCAg                <  1:388930/136‑1 (MQ=255)
                                                                                                                    ccTATATCGTCCAGGGCCCCGGACACTGCGGCAGTTGCCATACGCCACGTGGCCTGGCCTTCAATGAAAAGGCGCTGGATGACAGCGGCAAGCCGTTCCTGTCCGGCGCACTGCTCGACGGCTGGTATGCACCCAg                <  1:430467/136‑1 (MQ=255)
                                                                                                                                aGGGCCCCGGACACTGCGGCAGTTGCCATACGCCACGTGGCCTGGCCTTCAATGAAAAGGCGCTGGATGACAGCGGCAAGCCGTTCCTGTCCGGCGCACTGCTCGACGGCTGGTATGCACCCAGCCTACGCGCCGa    >  2:383015/1‑136 (MQ=255)
                                                                                                                                  ggCCCAGGACACTGCGGCAGTTGCCATACGCCACGTGGCCTGGCCTTCAATGAAAAGGCGCTGGATGACAGCGGCAAGCCGTTACTGTCCGGCGCACTGCTCGACGGCTGGTATGCACCAAGCCTACGCGCCGATc  >  1:311664/1‑136 (MQ=255)
                                                                                                                                       |                                                                                                                                  
CGACATCCCCTGGCCGCTGAACCTGCGCTGGCCCATCGCCTTGTGGAACGGCCTGTTCGCCGCCACTACGCCTTACACCGACAAGGCCGGGCAGGATGCACAGTGGAACCGTGGCGCCTATATCGTCCAGGGCCCCGGACACTGCGGCAGTTGCCATACGCCACGTGGCCTGGCCTTCAATGAAAAGGCGCTGGATGACAGCGGCAAGCCGTTCCTGTCCGGCGCACTGCTCGACGGCTGGTATGCACCCAGCCTACGCGCCGATC  >  NC_002947/4125054‑4125319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑