Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,494,386	G→T	26.6%	P824Q (CCA→CAA)	PP_3982 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,494,386	0	G	T	26.6%	31.4 / 2.8	15	P824Q (CCA→CAA)	PP_3982	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (4/7);  new base T (2/2);  total (6/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CGACATCTTCAACCAAGCCCGCATCAAAATCTTCTCGATCGATTTCCTCATGGGTGCCCTTGTTCAGATACCTCCAGATCAAGGTCTGCTCGCCGATTCCGAGGATTCGAACCAGTGCATCGATCAGTGGCTGCTTGTCTGCATGCTCAAACGTGTTGGCTCCTCGAAGCTTTGAACAGATAGATGCGCAGAGGTTACTCAGGCCAGGTTGATCTCCGTCACGAGCCAGCTGGACGGTCAAGACACCCTGACTGCATCTTCCCA  >  NC_002947/4494258‑4494521                                                                                                                                 |                                                                                                                                        cgacATCTTCAACCAAGCCCGCATCAAAATCTTCTCGATCGATTTCCTCATGGGTGCCCTTGTTCAGATACCTCCAGATCAAGGTCTGCTCGCCGATTCCGAGGATTCGAACCAGTGCATCGATCAGTGGCTGCtt                                                                                                                                  >  2:400117/1‑136 (MQ=255)                                     gatcgatTTCCTCATTGGTGCCCTTGTTCAGATACCTCCAGATCAAGGTCTGCTCGCCGATTCCGAGGATTCGAACCAGTGCATCGATCAGTTGCTGCTTGTCTGCATGCTCAAACGTGTTGGCTCCTCGAAGCtt                                                                                              >  1:377102/1‑136 (MQ=255)                                     gatcgatTTCCTCATTGGTGCCCTTGTTCAGATAACTCCAGATCAAGGTCTGCTAGCCGATTAAGAGGATTCGAACCAGTGCATCGATAAGTTGCTCCTTGTCTGCATGCTCAAACGAGTTGGCTCCTCGAAGCtt                                                                                              >  1:377101/1‑136 (MQ=255)                                     gatcgatTTCCTCATGGGTGCCCTTGTTCAGATACCTCCAGATCAAGGTCTGCTCGCCGATTCCGAGGATTCGAACCAGTGCATCGATCAGTGGCTGCTTGTCTGCATGCTCAAACGTGTTGGCTCCTCGAAGCtt                                                                                              <  1:488507/136‑1 (MQ=255)                                                   tGGGTGCCCTTGTTCAGATACCTCCAGATCAAGGTCTGCTCGCCGATTCCGAGGATTCGAACCAGTGCATCGATCAGTGGCTGCTTGTCTGCATGCTCAAACGTGTTGGCTCCTCGAAGCTTTGAACAGATAGATg                                                                                <  2:382947/136‑1 (MQ=255)                                                       tGCCCTTGTTCAGATACCTCCAGATCAAGGTCTGCTCGCCGATTCCGAGGATTCGAACCAGTGCATCGATCAGTGGCTGCTTGTCTGCATGCTCAAACGTGTTGGCTCCTCGAAGCTTTGAACAGATAGATGCGCa                                                                            <  2:359840/136‑1 (MQ=255)                                                            ttGTTCAGATACCTCCAGATCAAGGTCTGCTCGCCGATTCCGATGATTCGAACCAGTGCATCGATCAGTGgc                                                                                                                                       >  1:365347/1‑72 (MQ=255)                                                            ttGTTCAGATACCTCCAGATCAAGGTCTGCTCGCCGATTCCGATGATTCGAACCAGTGCATCGATCAGTGgc                                                                                                                                       <  2:365347/72‑1 (MQ=255)                                                                 cAGATACCTCCAGATCAAGGTCTGCTCGCCGATTCCGAGGATTCGAACCAGTGCATCGATCAGTTGCTGCTTGTCTGCATGCTCAAACGTGTTGGCTCCTCGAAGCTTTGAACAGATAGATGCGCAGAGGTTACTc                                                                  <  2:377102/136‑1 (MQ=255)                                                                 cAGATACCTCCAGATCAAGGTCTGCTCGCCGATTCCGAGGATTCGAACCAGTGCATCGATCAGTGGCTGCTTGTCTGCATGCTCAAACGTGTTGGCTCCTCGAAGCTTTGAACAGATAGATGCGCAGAGGTTACTc                                                                  <  1:331822/136‑1 (MQ=255)                                                                 cAGATACCTCCAGATCAAGGTCTGCTCGCCGATTCCGAGGATTCGAACCAGTGCAGCGATCAGTTGCTGCTTGTCTGCATGCTCAAACGTGTTGGCTCCTCGAAGCTTTGAACAGATAGATGCGCAGAGGTTACTc                                                                  <  2:377101/136‑1 (MQ=255)                                                                              ttCAAGGTCTGCTCGCCGATTCCGAGGATTCGAACCAGTGCATCGATCAGTGGCTGCTTGTCTGCATGCTCAAACGTGTTGGCTCCTCGAAGCTTTGAACAGATAGATGCGCAGAGGTTACTCAGGCCAGGTTGAt                                                     <  1:326772/135‑1 (MQ=255)                                                                                     tCTGCTCGCCGATTCCGAGGATTCGAACCAGTGCATCGATCAGTGGCTGCTTGTCTGCATGCTCAAACGTGTTGGCTCCTCGAAGCTTTGAACAGATAGATGCGCAGAGGTTACTCAGGCCAGGTTGATCTCCGTc                                              >  2:479232/1‑136 (MQ=255)                                                                                           cGCCGATTCCGAGGATTCGAACCAGTGCATCGATCAGTGGCTGCTTGTCTGCATGCTCAAACGTGTTGGCTCCTCGAAGCTTTGAACAGATAGATGCGCAGAGGTTACTCAGGCCAGGTTGATCTCCGTCACGAGc                                        >  1:113586/1‑136 (MQ=255)                                                                                               gATTCCGAGGATTCGAACCAGTGCATCGATCAGTGGCTGCTTGTCTGCATGCTCAAACGTGTTGGCTCCTCGAAGCTTTGAACAGATAGATGCGCAGAGGTTACTCAGGCCAGGTTGATCTCCGTCACGAGCCAGc                                    <  2:417941/136‑1 (MQ=255)                                                                                                                                 ggCTGCTTGTCTGCATGCTCAAACGTGTTGGCTCCTCGAAGCTTTGAACAGATAGATGCGCAGAGGTTACTCAGGCCAGGTTGATCTCCGTCACGAGCCAGCTGGACGGTCAAGACACCCTGACTGCATCTTCCCa  >  2:485881/1‑136 (MQ=255)                                                                                                                                 |                                                                                                                                        CGACATCTTCAACCAAGCCCGCATCAAAATCTTCTCGATCGATTTCCTCATGGGTGCCCTTGTTCAGATACCTCCAGATCAAGGTCTGCTCGCCGATTCCGAGGATTCGAACCAGTGCATCGATCAGTGGCTGCTTGTCTGCATGCTCAAACGTGTTGGCTCCTCGAAGCTTTGAACAGATAGATGCGCAGAGGTTACTCAGGCCAGGTTGATCTCCGTCACGAGCCAGCTGGACGGTCAAGACACCCTGACTGCATCTTCCCA  >  NC_002947/4494258‑4494521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑