| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,501,020 | G→T | 16.0% | E113* (GAA→TAA) | PP_3987 → | site‑specific recombinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,501,020 | 0 | G | T | 16.0% | 76.2 / 2.2 | 25 | E113* (GAA→TAA) | PP_3987 | site‑specific recombinase |
| Reads supporting (aligned to +/- strand): ref base G (17/4); new base T (2/2); total (19/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
GACGCAATCACTCCTCAAATAGTCGCTCAGTACCGAGACAAGCGATCAGGCAAAGTTCGCGCCAACCGAGAGATATCACTTCTATCGCACATTTATAATATGGCGCGGGAATGGGGCATTACCGACAGGGAAAACCCTGCTGCTGGCGTGCGCAAGAACAAAGAGCGACCCCGTGACTTCTATGCTGGACCGGAGATCTGGAACGCCGTTTACGATCGTGCTGCCAGCGAGCTACGCGACG > NC_002947/4500912‑4501152 | gACGCAATCACTCCTCAAATAGTCGCTCAGTACCGAGACAAGCGATCAGGCAAAGTTCGCGCCAACCGAGAGATATCACTTCTATCGCACATTTATAATATGGCGCGGTAATGGGGCATTACCGACAGGGAAAAcc > 1:251392/1‑136 (MQ=255)gACGCAATCACTCCTCAAATAGTCGCTCAGTACCGAGACAAGCGATCAGGCAAAGTTCGCGCCAACCGAGAGATATCACTTCTATCGCACATTTATAATATGGCGCGGTAATGGGGCATTACCGACAGGGAAAAcc > 1:334330/1‑136 (MQ=255)gACGCAATCACTCCTCAAATAGTCGCTCAGTACCGAGACAAGCGATCAGGCAAAGTTCGCGCCAACCGAGAGATATCACTTCTATCGCACATTTATAATATGGCGCGGGAATGGGGCATTACCGACAGGGAAAAcc > 1:170717/1‑136 (MQ=255)gACGCAATCACTCCTCAAATAGTCGCTCAGTACCGAGACAAGCGATCAGGCAAAGTTCGCGCCAACCGAGAGATATCACTTCTATCGCACATTTATAATATGGCGCGGGAATGGGGCATTACCGACAGGGAAAAcc > 1:61588/1‑136 (MQ=255) aCTCCTCAAATAGTCGCTCAGTACCGAGACAAGCGATCAGGCAAAGTTCGCGCCAACCGAGAGATATCACTTCTATCGCACATTTATAATATGGCGCGGGAATGGGGCATTACCGACAGGGAAAACCctgctgctg < 1:463969/136‑1 (MQ=255) gCTCAGTACCGAGACAAGCGATCAGGCAAAGTTCGCGCCAACCGAGAGATATCACTTCTATCGCACATTTATAATATGGCGCGGGAATGGGGCATTACCGACAGGGAAAACCCTGCTGCTGGCGTGCGAAAGAACa > 2:200464/1‑136 (MQ=255) gCTCAGTACCGACACAAGCGATCAGGCAAAGTTCGCGCCAACCGAGAGATATCACTTCTATCGCACATTTATAATATGGCGCGGGAATGGGGCATTACCGACAGGGAAAACCCTGCTGCTGGCGTGCGCAAGAACa > 2:391791/1‑136 (MQ=255) tCAGTACCGAGACAAGCGATCAGGCAAAGTTCGCGCCAACCGAGAGATATCACTTCTATCGCACATTTATAATATGGCGCGGGAATGGGGCATTACCGACAGGGAAAACCCTGCTGCTGGCGTGCGCAAGAACaaa > 1:171898/1‑136 (MQ=255) tCAGTACCGAGACAAGCGATCAGGCAAAGTTCGCGCCAACCGAGAGATATCACTTCTATCGCACATTTATAATATGGCGCGGGAATGGGGCATTACCGACAGGGAAAACCCTGCTGCTGGCGTGCGCAAGAACaaa > 1:173333/1‑136 (MQ=255) agaCAAGCGATCAGGCAAAGTTCGCGCCAACCGAGAGATATCACTTCTATCGCACATTTATAATATGGCGCGGGAATGGGGCATTACCGACAGGGAAAACCCTGCTGCTGGCGTGCGCAAGAACAAAGAGCGAccc < 2:364316/136‑1 (MQ=255) cAAGCGATCAGGCAAAGTTCGCGCCAACCGAGAGATATCACTTCTATCGCACATTTATAATATGGCGCGGGAATGGGGCATTACCGACAGGGAAAACCctg < 2:503622/101‑1 (MQ=255) cAAGCGATCAGGCAAAGTTCGCGCCAACCGAGAGATATCACTTCTATCGCACATTTATAATATGGCGCGGGAATGGGGCATTACCGACAGGGAAAACCctg > 1:503622/1‑101 (MQ=255) aaGCGATCAGGCAAAGTTCGCGCCAACCGAGAGATATCACTTCTATCGCACATTTATAATATGGCGCGGGAATGGGGCATTACCGACAGGGAAAACCCTGCTGCTGGCGTGCGCAAGAACAAAGAGCGCCCCCGTg > 2:85578/1‑136 (MQ=255) gATCAGGCAAAGTTCGCGCCAACCGAGAGATATCACTTCTATCGCACATTTATAATATGGCGCGGGAATGGGGCATTACCGACAGGGAAAACCCTGCTGCTGGCGTGCGCAAGAACAAAGAGCGACCCCGTGACtt > 2:423297/1‑136 (MQ=255) aCCGAGAGATATCACTTCTATCGCACATTTATAATATGGCGCGGTAATGGGGCATTACCGACAGGGAAAACCCTGCTGCTGGCGTGCGCAAGAACAAAGAGCGACCCCGTGACTTCTATGCTGGACCGGAGATCTg < 2:251392/136‑1 (MQ=255) aCCGAGAGATATCACTTCTATCGCACATTTATAATATGGCGCGGTAATGGGGCATTACCGACAGGGAAAACCCTGCTGCTGGCGTGCGCAAGAACAAAGAGCGACCCCGTGACTTCTATGCTGGACCGGAGATCTg < 2:334330/136‑1 (MQ=255) cGAGAGATATCACTTCTATCGCACATTTATAATATGGCGCGGGAATGGGGCATTACCGACAGGGAAAACCCTGCTGCTGGCGTGCGCAAGAACAAAGAGCGACCCCGTGACTTCTATGCTGGACCGGAGATCTGGa < 1:415796/136‑1 (MQ=255) aCTTCTATCGCACATTTATAATATGGCGCGGGAATGGGGCATTACCGACAGGGAAAACCCTGCTGCTGGCGTGCGCAAGAACAAAGAGCGACCCCGTGACTTCTATGCTGGACCGGAGATCTGGAACGCCGTTTAc > 1:396343/1‑136 (MQ=255) aCTTCTATCGCACATTTATAATATGGCGCGGGAATGGGGCATTACCGACAGGGAAAACCCTGCTGCTGGCGTGCGCAAGAACAAAGAGCGACCCCGTGACTTCTATGCTGGACCGGAGATCTGGAACGCCGTTTAc > 1:140981/1‑136 (MQ=255) tttATAATATGGCGCTGGAATGGGGCATTACCGACAGGGAAAACCCTGCTGCTGGCGTGCGCAAGAACAAAGAGCGACCCCGTGACTTCTATGCTGGACCGGAGATCTGGAACGCCGTTTACGATCGTGCTGCCAg > 1:539561/1‑136 (MQ=255) cgcgGGAATGGGGCATTACCGACAGGGAAAACCCTGCTGCTGGCGTGCGCAAGAACAAAGAGCGACCCCGTGACTTCTATGCTGGACCGGAGATCTGGAACGCCGTTTACGATCGTGCTGCCAGCGAGCTACGCGa > 1:332792/1‑136 (MQ=255) cgcgGGAATGGGGCATTACCGACAGGGAAAACCCTGCTGCTGGCGTGCGCAAGAACAAAGAGCGACCCCGTGACTTCTATGCTGGACCGGAGATCTGGAACGCCGTTTACGATCGTGCTGCCAGCGAGCTACGCGa > 1:277495/1‑136 (MQ=255) cgGGAATGGGGCATTACCGACAGGGAAAACCCTGCTGCTGGCGTGCGCAAGAACAAAGAGCGACCCCGTGACTTCTATGCTGGACCGGAGATCTGGAACGCCGTTTACGATCGTGCTGCCAGCGAGCTACGCGACg > 1:512222/1‑136 (MQ=255) cgGGAATGGGGCATTACCGACAGGGAAAACCCTGCTGCTGGCGTGCGCAAGAACAAAGAGCGACCCCGTGACTTCTATGCTGGACCGGAGATCTGGAACGCCGTTTACGATCGTGCTGCCAGCGAGCTACGCGACg > 2:257544/1‑136 (MQ=255) cgGGAATGGGGCATTACCGACAGGGAAAACCCTGCTGCTGGCGTGCGCAAGAACAAAGAGCGACACAGTGACTTCTATGCTGGACCGGAGATCTGGAACGCCGTTTACGATCGTGCTGCCAGCGAGCTACGCGACg > 2:194392/1‑136 (MQ=255) | GACGCAATCACTCCTCAAATAGTCGCTCAGTACCGAGACAAGCGATCAGGCAAAGTTCGCGCCAACCGAGAGATATCACTTCTATCGCACATTTATAATATGGCGCGGGAATGGGGCATTACCGACAGGGAAAACCCTGCTGCTGGCGTGCGCAAGAACAAAGAGCGACCCCGTGACTTCTATGCTGGACCGGAGATCTGGAACGCCGTTTACGATCGTGCTGCCAGCGAGCTACGCGACG > NC_002947/4500912‑4501152 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |