| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,525,911 | C→A | 14.8% | G274C (GGT→TGT) | icd ← | NADP(+)‑specific isocitrate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,525,911 | 0 | C | A | 14.8% | 82.8 / 2.1 | 27 | G274C (GGT→TGT) | icd | NADP(+)‑specific isocitrate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (13/10); new base A (2/2); total (15/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01 | |||||||||||
TGCGGCCAGGGCATCGGACAGGTAGTCACCGTTGAGGTTGAGGGTGGCGATCACGTCGTACTCGGCCGGGCGTAGCAGGATCTGCTGCAGCATGGCGTCGGCGATGGCGTCCTTGACGATGACCTCGCGACCGGTCTTGGGGTTCTTGAACTTCATCCAAGGGCCGCCATCAAGCAGTTCAGCGCCGAACTCGTCACGCGCCACCTCGTACCCCCAGTCCTTGAAGGCACCCTCGGTGAACTTCATGATGTTGCCCTTGTGC > NC_002947/4525780‑4526041 | tGCGGCCAGGGCATCGGACAGGTAGTCACCGTTGAGGTTGAGGGTGGCGATCACGTCGTACTCGGCCGGGCGTAGCAGGATCTGCTGCAGCATGGCGTCGGCGATGGCGTCCTTGACGATGACCTCGCGACCGGtg > 1:424823/1‑135 (MQ=255) tAGTCACCGTTGAGGTTGAGGGTGGCGATCACGTCGTACTCGGCCGGGCGTAGCAGGATCTGCTGCAGCATGGCGTCGGCGATGGCGTCCTTGACGATGACCTCGCGACCGGTCTTGGGGTTCTTGAACTTCATcc > 2:274039/1‑136 (MQ=255) cccGTGGAGGTTGAGGGGGGCGATCACGTCGTACTCGGCCGGGCGTAGCAGGATCTGCTGCAGCATGGCGTCGGCGATGGCGTCCTTGACGATGACCTCGCGACCGGTCTTGGGGTTCTTGAACTTCATCCAAggg < 2:464547/135‑1 (MQ=255) cGTTGAGGTTGAGGGTGGCGATCACGTCGTACTCGGCCGGGCGTAGCAGGATCTGCTGCAGCATGGCGTCGGCGATGGCGTCCTTGACGATGACCTCGCGACCGGTCTTGGGGTTCTTGAACTTCATCCAAGGgcc > 1:521922/1‑136 (MQ=255) gttgaggttgagGGTGGCGATCACGTCGTACTCGGCCGGGCGTAGCAGGATCTGCTGCAGCATGGCGTCGGCGATGGCGTCCTTGACGATGACCTCGCGACCGGTCTTGGGGTTCTTGAACTTCATCCAAGGgccg > 1:488718/1‑136 (MQ=255) ggTGGCGATCACGTCGTACTCGGCCGGGCGTAGCAGGATCTGCTGCAGCATGGCGTCGGCGATGGCGTCCTTGACGATGACCTCGCGACCGGTCTTGGGGTTCTTGAACTTCATCCAAGGGCCGCCATCAAGCAGt > 2:363947/1‑136 (MQ=255) ggTGGCGATCACGTCGTACTCGGCCGGGCGTAGCAGGATCTGCTGCAGCATGGCGTCGGCGATGGCGTCCTTGACGATGACCTCGCGACCGGTCTTGGGGTTCTTGAACTTCATCCAAGGGCCGCCATCAAGCAGt > 2:357913/1‑136 (MQ=255) gtACTCGGCCGGGCGTAGCAGGATCTGCTGCAGCATGGCGTCGGCGATGGCGTCCTTGACGATGACCTCGCGACCGGTCTTGGGGTTCTTGAACTTCATCCAAGGGCCGCCATCAAGCAGTTCAGCGCCGAACTCg > 1:325793/1‑136 (MQ=255) cGTAGCAGGATCTGCTGCAGCATGGCGTCGGCGATGGCGTCCTTGACGATGACCTCGCGACCGGTCTTGGGGTTCTTGAACTTCATCCAAg < 2:441620/91‑1 (MQ=255) cGTAGCAGGATCTGCTGCAGCATGGCGTCGGCGATGGCGTCCTTGACGATGACCTCGCGACCGGTCTTGGGGTTCTTGAACTTCATCCAAg > 1:441620/1‑91 (MQ=255) ctggAGCTGCTGCAGCATGGCGTGGGCGATGGCGTCCTTGACGATGACCTCGCGACCGGTCTTGGGGTTCTTGAACTTCATCCAAGGGCCGCCATCAAGCAGTTCAGCGCCGAACTCGTCACGCGCCACCTCGTAc < 1:194837/134‑1 (MQ=255) aGGATCTGCTGCAGCATGGCGTCGGCGATGGCGTCCTTGACGATGACCTCGCGACCGGTCTTGGGGTTCTTGAACTTCATCCAAGGGCCGCCATCAAGCAGTTCAGCGCCGAACTCGTCACGCGCCACCTCGTAcc > 2:378660/1‑136 (MQ=255) gATCTGCTGCAGCATGGCGTCGGCGATGGCGTCCTTGACGATGACCTCGCGACCGGTCTTGGGGTTCTTGAACGTCATCCAAGGGCCGCCATCAAGCAGTTCAGCGCCGACCTCGTCCCGCGCCACCTCGTAcccc > 2:486067/1‑136 (MQ=255) tgctgcAGCATGGCGTCGGCGATGGCGTCCTTGACGATGACCTCGCGACAGGTCTTGGGGTTCTTGAACTTCATCCAAGGGCCGCCATCAAGCAGTTAAGCGCCGAACTCGTCACGCGCCAcc < 2:202863/123‑1 (MQ=255) tgctgcAGCATGGCGTCGGCGATGGCGTCCTTGACGATGACCTCGCGACAGGTCTTGGGGTTCTTGAACTTCATCCAAGGGCCGCCATCAAGCAGTTAAGCGCCGAACTCGTCACGCGCCAcc < 2:348755/123‑1 (MQ=255) tgctgcAGCATGGCGTCGGCGATGGCGTCCTTGACGATGACCTCGCGACAGGTCTTGGGGTTCTTGAACTTCATCCAAGGGCCGCCATCAAGCAGTTAAGCGCCGAACTCGTCACGCGCCAcc > 1:348755/1‑123 (MQ=255) tgctgcAGCATGGCGTCGGCGATGGCGTCCTTGACGATGACCTCGCGACAGGTCTTGGGGTTCTTGAACTTCATCCAAGGGCCGCCATCAAGCAGTTAAGCGCCGAACTCGTCACGCGCCAcc > 1:202863/1‑123 (MQ=255) gctgcAGCATGGCGTCGGCGATGGCGTCCTTGACGATGACCTCGCGACCGGTCTTGGGGTTCTTGAACTTCATCCAAGGGCCGCCATCAAGCAGTTCAGCGCCGAACTCGTCACGCGCCACCTCGTACCCCCAGTc > 1:11262/1‑136 (MQ=255) gCGTCGGCGATGGCGTCCTTGACGATGACCTCGCGACCGGTCTTGGGGTTCTTGAACTTCATCCAAGGGCCGCCATCAAGCAGTTCAGCGCCGAACTCGTCACGCGCCACCTCGTACCCCCAGTCCTTGAAGGCAc < 2:398143/136‑1 (MQ=255) cGTCGGCGATGGCGTCCTTGACGATGACCTCGCTACCGGTCTTGGGGTTCTTGAACTTCATCCAAGGGCCGCCATCAAGCAGTTCAGCGCCGAACTCGTCACGCGCCACCTCGTACCCCCAGTCCTTGAAGGCAcc > 1:90266/1‑136 (MQ=255) gTCGGCGATGGCGTCCTTGACGATGACCTCGCGACCGGTCTTGGGGTTCTTGAACTTCATCCAAGGGCCGCCATCAAGCAGTTCAGCGCCGAACTCGTCACGCGCCACCTCGTACCCACAGTCCTTGAAGGCAccc < 1:236061/136‑1 (MQ=255) ggCGATGGCGTCCTTGACGATGACCTCGCGACCGGTCTTGGGGTTCTTGAACTTCATCCAAGGGCCGCCATCAAGCAGTTCAGCGCCGAACTCGTCACGCGCCACCTCGTACCCCCAGTCCTTGAAGGCACCCTCg < 1:391258/136‑1 (MQ=255) gCGATGGCGTCCTTGACGATGACCTCGCGACCGGTCTTGGGGTTCTTGAACATCATCCAAGGGCCGCCATCAAGCAGTTCAGCGCCGAACTCGTCACGCGCCACCTCGTACCCCCAGTCCTTGAAGGCACCCTCgg < 1:307681/136‑1 (MQ=255) gCGATGGCGTCCTTGACGATGACCTCGCGACCGGTATAGGGGATCTTGAACTTCATCCAAGGGCCGCCATCAACCAGTTCAGCTCCGAACTCGTCACGCGCAACATCGTACCCACACACCTTGAACGCACCCTCgg > 1:375172/1‑136 (MQ=255) gATGACCTCGCGACCGGTCTTGGGGTTCTTGAACTTCATCCAAGGGCCGCCATCAAGCAGTTCAGCGCCGAACTCGTCACGCGCCACCTCGTACCCCCAGTCCTTGAAGGCACCCTAGGTGAACTTCATGATGTTg < 2:424823/136‑1 (MQ=255) tGACCTCGCGACCGGTCTTGGGGTTCTTGAACTTCATCCAAGGGCCGCCATCAAGCAGTTCAGCGCCGAACTCGTCACGCGCCACCTCGTACCCCCAGTCCTTGAAGGCACCCTCGGTGAACTTCATGATGTTGcc < 2:324008/136‑1 (MQ=255) gcgACCGGTCTTGGGGTTCTTGAACTTCATCCAAGGGCCGCCATCAAGCAGTTCAGCGCCGAACTCGTCACGCGCCACCTCGTACCCCCAGTCCTTGAAGGCACCCTCGGTGAACTTCATGATGTTGCCCTTGTGc < 2:184301/136‑1 (MQ=255) | TGCGGCCAGGGCATCGGACAGGTAGTCACCGTTGAGGTTGAGGGTGGCGATCACGTCGTACTCGGCCGGGCGTAGCAGGATCTGCTGCAGCATGGCGTCGGCGATGGCGTCCTTGACGATGACCTCGCGACCGGTCTTGGGGTTCTTGAACTTCATCCAAGGGCCGCCATCAAGCAGTTCAGCGCCGAACTCGTCACGCGCCACCTCGTACCCCCAGTCCTTGAAGGCACCCTCGGTGAACTTCATGATGTTGCCCTTGTGC > NC_002947/4525780‑4526041 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |