| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,557,342 | C→A | 21.0% | G87V (GGG→GTG) | rutR ← | transcriptional regulator, TetR family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,557,342 | 0 | C | A | 21.0% | 38.9 / 2.6 | 19 | G87V (GGG→GTG) | rutR | transcriptional regulator, TetR family |
| Reads supporting (aligned to +/- strand): ref base C (10/5); new base A (2/2); total (12/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.03e-01 | |||||||||||
TGGGTAGACGCCCAGATCATATACAGCAACGCCGAAGGCTCTACCGGCAGAATACGCCCCTCTTCCACCCAGCAGCGGATCTTGGCTTCTTTCATCTTCGCCCAGGGCACCAGGCTTTCGTCGAGGTTAACCCCCAGCACCGGCGCCCCGTGCAGCATCTCTTCGGCCCAGATCTTCGAGCCCAGCGGCCGCGAGCGCGAATGGCCCATCTTCGCCCGGATGTAGCTGGTCAGCACCACACGCGGGTCGTCGAAGTTC > NC_002947/4557210‑4557467 | tGGGTAGACGCCCAGATCATATACAGCAACGCCGAAGGCTCTACCGGCAGAATACGCCCCTCTTCCACCCAGCAGCGGATCTTGGCTTCTTTCATCTTCGCCCAGGGCACCAGGCTTTCGTCGAGGTTAAcccccc > 1:285548/1‑135 (MQ=255) aTCATATACAGCAACGCCGAAGGCTCTACCGGCAGAATACGCCCCTCTTCCACCCAGCAGCGGATCTTGGCTTCTTTCATCTTCGCCCAGGGCACCAGGCTTTCGTCGAGGTTAACCCCCAGCACCGGCGCCCCgg > 1:38348/1‑135 (MQ=255) aTCATATACAGCAACGCCGAAGGCTCTACCGGCAGAATACGCCCCTCTTCCACCCAGCAGCGGATCTTGGCTTCTTTCATCTTCGCCCAGGGCACCAGGCTTTCGTCGAGGTTAACCCCCAGCACCGGCGCCCCGt > 1:427072/1‑136 (MQ=255) aGCAACGCCGAAGGCTCTACCGGCAGAATACGCCCCTCTTCCACCCAGCAGCGGATCTTGGCTTCTTTCATCTTCGCCCAGGGCACCAGGCTTTCGTCGAGGTTAACCCCCAGCACCGGCGCCCCGTGCAGCAtct < 1:358420/136‑1 (MQ=255) aGCAACGCCGAAGGCTCTACCGGCAGAATACGCCCCTCTTCCACCCAGCAGCGGATCTTGGCTTCTTTCATCTTCGCCCAGGGCACCAGGCTTTCGTCGAGGTTAACCCCCAGCACCGGCGCCCCGTGCAGCAtct < 1:441528/136‑1 (MQ=255) cGAAGGCTCTACCGGCAGAATACGCCCCTCTTCCACCCAGCAGCGGATCTTGGCTTCTTTCATCTTCGCCCAGGGCACCAGGCTTTCGTCGAGGTTAACCCCCAGCACCGGCGCCCCGTGCAGCATCTCTTCGGcc > 1:2642/1‑136 (MQ=255) cGAAGGCTCTACCGGCAGAATACGCCCCTCTTCCACCCAGCAGCGGATCTTGGCTTCTTTCATCTTCGCCCAGGGCACCAGGCTTTCGTCGAGGTTAACCCCCAGCACCGGCGCCCCGTGCAGCATCTCTTCGGcc < 2:436467/136‑1 (MQ=255) cGAAGGCTCTACCGGCAGAATACGCCCCTCTTCCACCCAGCAGCGGATCTTGGCTTCTTTCATCTTCGCCCAGGGCACCAGGCTTTCGTCGAGGTTAACCCCCAGCACCGGCGCCCCGTGCAGCATCTCTTCGGcc > 1:117458/1‑136 (MQ=255) cGAAGGCTCTACCGGCAGAATACGCCCCTCTTCCACCCAGCAGCGGATCTTGGCTTCTTTCATCTTCGCCCAGGGCACCAGGCTTTCGTCGAGGTTAACCACCAGCACCGGCGCCCCGTGCAGCATCTCTTCGGcc > 1:471408/1‑136 (MQ=255) cGAAGGCTCTACCGGCAGAATACGCCCCTCTTCCACCCAGCAGCGGATCTTGGCTTCTTTCATCTTCGCCCAGCGCACCAGGCTTTCGTCGAGGTTAACCACCAGCACCGGCGCCCCGTGCAGCATCTCTTCggac > 1:335853/1‑134 (MQ=255) gCTCTACCGGCAGAATACGCCCCTCTTCCACCCAGCAGCGGATCTTGGCTTCTTTCATCTTCGCCCAGGGCACCAGGCTTTCGTCGAGGTTAACCCCCAGCACCGGCGCCCCGTGCAGCATCTCTTCGGCCCAGAt > 1:44083/1‑136 (MQ=255) gCTCTACCGGCAGAATACGCCCCTCTTCCACCCAGCAGCGGATCTTGGCTTCTTTCATCTTCGCCCAGGGCACCAGGCTTTCGTCGAGGTTAACCCCCAGCACCGGCGCCCCGTGCAGCATCTCTTCGGCCCAGAt > 1:278865/1‑136 (MQ=255) cGGCAGAATACGCCCCTCTTCCACCCAGCAGCGGATCTTGGCTTCTTTCATCTTCGCCCAGGGCACCAGGCTTTCGTCGAGGTTAACCCCCAGCACCGGCGCCCCGTGCAGCATCTCTTCGGCCCAGATCTTCGAg > 1:4980/1‑136 (MQ=255) tCCACCCAGCAGCGGATCTTGGCTTCTTTCATCTTCGCCCAGGGCACCAGGCTTTCGTCGAGGTTAACCCCCAGCACCGGCGCCCCGTGCAGCATCTCTTCGGCCCAGATCTTCGAGCCCAGCGGCCGCGAGcgcg > 2:290565/1‑136 (MQ=255) cttcttTCATCTTCGCCCAGGGCACCAGGCTTTCGTCGAGGTTAACCCCCAGCACCGGCGCCCCGTGCAGCATCTCTTCGGCCCAGATCTTCGAGCCCAGCGGCCGCGAGCGCGAATGGCCCATCTTCGCCCGGAt > 2:490807/1‑136 (MQ=255) ttcttTCATCTTCGCCCAGGGCACCAGGCTTTCGTCGAGGTTAACCCCCAGCACCGGCGCCCCGTGCAGCATCTCTTCGGCCCAGATCTTCGAGCCCAGCGGCCGCGAGCGCGAATGGCCCATCTTCGCCCGGATg < 2:278865/136‑1 (MQ=255) aTCTTCGCCCAGGGCACCAGGCTTTCGTCGAGGTTAACCACCAGCACCGGCGCCCCGTGCAGCATCTCTTCGGCCCAGATCTTCGAGCCCTGCGGCCGCGAGCGCGAATGGCCCATCTTCGCCCGGATGTAGCTgg < 2:335853/136‑1 (MQ=255) aTCTTCGCCCAGGGCACCAGGCTTTCGTCGAGGTTAACCACCAGCACCGGCGCCCCGTGCAGCATCTCTTCGGCCCAGATCTTCGAGCCCAGCGGCCGCGAGCGCGAATGGCCCATCTTCGCCCGGATGTAGCTgg < 2:471408/136‑1 (MQ=255) aCCAGGCTTTCGTCGAGGTTAACCCCCAGCACCGGCGCCCCGTGCAGCATCTCTTCGGCCCAGATCTTCGAGCCCAGCGGCCGCGAGCGCGAATGGCCCATCTTCGCCCGGATGTAGCTGGTCAGCACCACAcgcg > 2:494570/1‑136 (MQ=255) gAGGTTAACCCCCAGCACCGGCGCCCCGTGCAGCATCTCTTCGGCCCAGATCTTCGAGCCCAGCGGCCGCGAGCGCGAATGGCCCATCTTCGCCCGGATGTAGCTGGTCAGCACCACACGCGGGTCGTCGAAGTtc < 2:44083/136‑1 (MQ=255) | TGGGTAGACGCCCAGATCATATACAGCAACGCCGAAGGCTCTACCGGCAGAATACGCCCCTCTTCCACCCAGCAGCGGATCTTGGCTTCTTTCATCTTCGCCCAGGGCACCAGGCTTTCGTCGAGGTTAACCCCCAGCACCGGCGCCCCGTGCAGCATCTCTTCGGCCCAGATCTTCGAGCCCAGCGGCCGCGAGCGCGAATGGCCCATCTTCGCCCGGATGTAGCTGGTCAGCACCACACGCGGGTCGTCGAAGTTC > NC_002947/4557210‑4557467 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |