| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,694,596 | G→T | 20.0% | G161C (GGC→TGC) | yejF → | microcin C ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,694,596 | 0 | G | T | 20.0% | 49.4 / 2.6 | 20 | G161C (GGC→TGC) | yejF | microcin C ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (13/3); new base T (2/2); total (15/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
AACGAAATCCTCCTGCTGCACAAGGGCCTGACGGGCAAGGAAGCGACTGCGCGCACCCTGGAGTTGCTGGATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCAGCGCCAGCGGGTAATGATCGCCATGGCGCTGGCCAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGACCACGGCGCTGGATGTGACCGTGCAGCTGAAGATCCTCGAGCTGCTCAAGGAATTGCAGGCGC > NC_002947/4694464‑4694731 | aaCGAAATCCTCCTGCTGCACAAGGGCCTGACGGGCAAGGAAGCGACTGCGCGCACCCTGGAGTTGCTGGATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGgcc > 2:91536/1‑136 (MQ=255) cGGGCAAGGAAGCGACTGCGCGCACCCTGGAGTTGCTGGATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCCCGAAATGTCGGGCGGCCACCGCCAGCCGGTAATGAACGCCATGGCGCt > 1:422597/1‑136 (MQ=255) gcACCCTGGAGTTGCTGGATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCAGCGCCAGCGGGTAATGATCGCCATGGCGCTGGCCAACGAGCCCGAgctgct > 1:276050/1‑136 (MQ=255) cTGGAGTTGCTGGATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCACCGCCAGCGGGTAATGATCGCCATGGCGCAGGCCCACGCGCCCGAGCAGCAGAttt > 2:283665/1‑135 (MQ=255) tGGATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCTGCCAGCGCCAGCGGGTAATGATCGCCATGGCGCTGGCCAACGAGCCCGAGCTGCTGATTGCCGACTAGcc > 1:16922/1‑136 (MQ=255) tGGATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCTGCCAGCGCCAGCGGGTAATGATCGCCATGGCGCTGGCCAACGAGCCCGAGCTGCTGATTGCCGACTAGcc > 1:384926/1‑136 (MQ=255) gATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCCGCGCCCGCGGGGAATGATAGCCATGGCGCTGGCCAACGCGCCCGCGCAGCTGATTGCCGACGAGCCgc > 1:32907/1‑135 (MQ=255) gATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCAGCGCCAGCGGGGAATGATCCCCATGGCGCAGGCCAACGAGCCCGAGCTGCAGATTGCcgccgcgccgc > 2:409207/1‑135 (MQ=255) gATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCAGCCCCCGCGGGTTATTATAGCCATGGCCCTGGGCAACGCGCCCGCGCTGCTGCTTTCCGACCAGCCgc > 2:346073/1‑135 (MQ=255) ggTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCAGCGCCAGCGGGTAATGATCGCCATGGCGCTGGCCAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGACCACg < 1:102600/136‑1 (MQ=255) ggTCGGCATACCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCAGCGCCAGCGGGTAATGATCGCCATGGCGCTGGCCAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGACCACg < 1:283665/136‑1 (MQ=255) gcGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCAGCGCCAGCGGGTAATGATCGCCATGGCGCTGGCCAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGACCACGgc < 2:395606/135‑1 (MQ=255) cgcAAGCGCCTCAAGGCCCTGCCCAACGAATTGTCGGGCGGCCAGCGCCAGCGGGTAATGATCGCCATGGCGCTGGCCAACGAGCCCGCGCTGCTGATTGCCGACGAGCCGACCACGGCGCTGGATGTGACCGTGc > 1:200251/1‑136 (MQ=255) aaGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCAGCCCCAGCGGGTAAAGATCGCCAAGGCGCTGGCCAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGACCACCGCGCGGGATGTGACCGTGCAGc > 1:511528/1‑136 (MQ=255) cccACGAATTGTCGGGCGGCCAGCGCCAGCGGGTAATGATCGCCAAGGCGCTGGCCAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGACCACGGCGCTGGATGTGACCGTGCAGCTGAAGATCCTCGAgctgct > 2:199549/1‑136 (MQ=255) cccACGAATTGTCGGGCGGCCAGCGCCAGCGGGTAATGATAGCCATGGCGCTGGCCAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGACCACGGCGCTGGATGTGACCGTGCAGCTGAAGATCCTCGAgctgct > 1:9392/1‑136 (MQ=255) tCGGGCTGCCAGCGCCAGCGGGTAATGATCGCCATGGCGCTGGCCAACGAGCCCGAGCTGCTGATTGCCGACTAGCCGACCACGGCGCTGGATGTGACCGTGCAGCTGAAGATCCTCGAGCTGCTCAAGGAATTGc < 2:16922/136‑1 (MQ=255) tCGGGCTGCCAGCGCCAGCGGGTAATGATCGCCATGGCGCTGGCCAACGAGCCCGAGCTGCTGATTGCCGACTAGCCGACCACGGCGCTGGATGTGACCGTGCAGCTGAAGATCCTCGAGCTGCTCAAGGAATTGc < 2:384926/136‑1 (MQ=255) cGGGCGGCCAGCGCCAGCGGGTAATGATCGCCATGGCGCTGGCCAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGACCACGGCGCTGGATTTGACCGTGCAGCTGAAGATCCTCGAGCTGCTCAAGGAATTGCa > 2:320810/1‑136 (MQ=255) cGGGCGGCCAGCGCCAGCGGGTAATGATCGCCATGGCGCTGGCCAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGACCACGGCGCTGGATGTGACCGTGCAGCTGAAGATCCTCGAGCTGCTCAAGGAATTGCa > 2:294902/1‑136 (MQ=255) ggcCAGCGCCAGCGGGTAATGATCGCCATGGCGCTGGCCAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGACCACGGCGCTTGATGTGACCGTGCAGCTGAAGATCCTCGAGCTGCTCAAGGAATTGCAGgcgc < 1:332853/136‑1 (MQ=255) | AACGAAATCCTCCTGCTGCACAAGGGCCTGACGGGCAAGGAAGCGACTGCGCGCACCCTGGAGTTGCTGGATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCAGCGCCAGCGGGTAATGATCGCCATGGCGCTGGCCAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGACCACGGCGCTGGATGTGACCGTGCAGCTGAAGATCCTCGAGCTGCTCAAGGAATTGCAGGCGC > NC_002947/4694464‑4694731 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |