| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,755,122 | G→T | 22.2% | P109T (CCG→ACG) | mqsA ← | type II toxin‑antitoxin MqsRA system antitoxin/DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,755,122 | 0 | G | T | 22.2% | 44.7 / 2.9 | 18 | P109T (CCG→ACG) | mqsA | type II toxin‑antitoxin MqsRA system antitoxin/DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base G (9/5); new base T (2/2); total (11/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
GGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGGAGAACGCATTGACCCCACCACCGAAGATTTCCCCCGCTTCACGCTGGTCGAGGTCGAACTTTCTGCGTATGGAGGCAATAAAGGAAGGATC > NC_002947/4754989‑4755239 | ggCAACAAAAAGCCCCCGGTTGTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGTTGGCGATCCAGCAGCGTGAGCAGTTTCACCAACACCACTGGCgggg > 1:52083/1‑136 (MQ=255)ggCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCgggg > 2:353267/1‑136 (MQ=255) aaaaGCCCCCGGTTCTCATTAGCCGGGGGCGTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGTGTTATGGATGGCGATCCAGCAGCTTTAGCAGTTTAACCAACGCCCCTGGCGGGGTGGTGtt > 2:24658/1‑136 (MQ=255) cATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACgg < 2:398684/136‑1 (MQ=255) aTGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACggg < 1:305014/136‑1 (MQ=255) aGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGTGGTGGTCTTGCCGTTTTCATAACGGgaga > 2:414856/1‑136 (MQ=255) aGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTAAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGTGGTGGTCTTGCCGTTTTCATAACAGgaga > 2:92907/1‑136 (MQ=255) gCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGGAGAACGCATTg > 2:127377/1‑136 (MQ=255) tATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGGAGAACGCATTGACCCCACCACCGAAGAttt < 1:396565/136‑1 (MQ=255) cAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGGAGAACGCATTGACCCCACCACCGAAGATTTCCCCCGc > 2:280525/1‑136 (MQ=255) gTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGGAGAACGCATTGACCCCACCACCGAAGAttt > 1:423737/1‑127 (MQ=255) gTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGGAGAACGCATTGACCCCACCACCGAAGAttt < 2:423737/127‑1 (MQ=255) gTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGGAGAACGCATTGACCCCACCACCGAAGATTTCCCCCGCTTCACGCTGGTCGAGGTCGaa > 2:539046/1‑136 (MQ=255) ggCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGGAGAACGCATTGACCCCACCACCGAAGATTTCCCCCGCTTCACGCTGGTCGAGGTCGAACTTTCTGCg < 2:87578/136‑1 (MQ=255) tCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGGAGAACGCATTGACCCCACCACCGAAGATTTCCCCCGCTTCACGCTGGTCGAGGTCGAACTTTCTGCGTATgg > 1:437261/1‑136 (MQ=255) tCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGGAGAACGCATTGACCCCACCACCGAAGATTTCCCCCGCTTCACGCTGGTCGAGGTCGAACTTTCTGCGTATgg > 1:128018/1‑136 (MQ=255) gCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGGAGAACGCATTTACCCCACCACCGAAGATTTCCCCCGCTTCACGCTGGTCGAGGTCGAACTTTCTGCGTATGGAGGCAATAaagga > 1:377212/1‑136 (MQ=255) gCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGGAGAACGCATTGACCCCACCACCGAAGATTTCCCCCGCTTCACGCTGGTCGAGGTCGAACTTTCTGCGTATGGAGGCAATAaagga > 2:513647/1‑136 (MQ=255) tCACCAACGCCACTGGCGTGGTGGTCTTGCCGTTTTCATAACGGGAGAACGCATTGACCCCACCACCGAAGATTTCCCCCGCTTCACGCTGGTCGAGGTCGAACTTTCTGCGTATGGAGGCAATAAAGGAAGGATc < 1:414856/136‑1 (MQ=255) tCACCAACGCCACTGGCGTGGTGGTCTTGCCGTTTTCATAACGGGAGAACGCATTGACCCCACCACCGAAGATTTCCCCCGCTTCACGCTGGTCGAGGTCGAACTTTCTGCGTATGGAGGCAATAAAGGAAGGATc < 1:92907/136‑1 (MQ=255) | GGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGGAGAACGCATTGACCCCACCACCGAAGATTTCCCCCGCTTCACGCTGGTCGAGGTCGAACTTTCTGCGTATGGAGGCAATAAAGGAAGGATC > NC_002947/4754989‑4755239 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |