| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,947,598 | C→A | 23.5% | A13A (GCC→GCA) | PP_4350 → | class V NifS/IscS family aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,947,598 | 0 | C | A | 23.5% | 36.8 / 2.8 | 17 | A13A (GCC→GCA) | PP_4350 | class V NifS/IscS family aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (7/6); new base A (2/2); total (9/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
GGCAGATCTGGGAGGGAGCGGGGAGCTCACGACCGAAGAAATATACAGCAAAGGCATCCATATCGTCACCTATAGAACTTGAGAGGCCAGCAATGCAACCAGACTCGGTAATCTATCTAGATGCTGCAGCCACTACTCCGTGCGCTGATGACGTCATAAAGGAAATGGCGAAATATGACAACATCAACTACGGAAACCCCTCATCTGCGCACATTCTAGGCCGTCTTGCACGGAGTGCTGTCTTTGAAAGTGCTTCACATATTT > NC_002947/4947468‑4947731 | ggCAGATCTGGGAGGGAGCGGGGAGCTCACGACCGAAGAAATATACAGCAAAGGCATCCATATCGTCACCTATAGAACTTGAGAGGCCAGCAATGCAACCAGACTCGGTAATCTATCTAGATGCTGCAGCCactac > 1:228374/1‑136 (MQ=255) aGATCTGGGAGGGAGCGGGGAGCTCACGACCGAAGAAATATACAGCAAAGGCATCCATATCGTCACCTATAGAACTTGAGAGGCCAGCAATGCAACCAGACTCGGTAATCTATCTAGATGCTGCAGCCACTACTcc < 1:496087/136‑1 (MQ=255) gATCTGGGAGGGAGCGGGGAGCTCACGACCGAAGAAATATACAGCAAAGGCATCCATATCGTCACCTATAGAACTTGAGAGGCCAGCAATGCAACCAGACTCGGTAATCTATCTAGATGCTGCAGCCACTACTccc > 2:116034/1‑135 (MQ=255) gagggagCGGGCAGCTCACGACCGAAGAAATATACAGCAAAGGCATCCATATCGTCACCTATAGAACTTGAGAGGCCAGCAATGCAACCAGACTCTGTAATCTATCTAGATGCTGCAGCCACTACTCCGTGCGCtg < 2:502567/136‑1 (MQ=255) aGGCATCCATATCGTCACCTATAGAACTTGAGAGGCCAGCAATGCAACCAGACTCGGTAATCTATCTAGATGCTGCAGCCACTACTCCGTGCGCTGATGACGTCATAAAGGAAATGGCGAAATATGACAACATCaa > 2:143332/1‑136 (MQ=255) aGGCATCCATATCGTCACCTATAGAACTTGAGAGGCCAGCAATGCAACCAGACTCGGTAATCTATCTAGATGCTGCAGCCACTACTCCGTGCGCTGATGACGTCATAAAGGAAATGGCGAAATATGACAACATCaa > 2:246857/1‑136 (MQ=255) atatCGTCACCTATAGAACTTGAGAGGCCAGCAATGCAACCAGACTCGGTAATCTATCTAGATGCTGCAGCCACTACTCCGTGCGCTGATGACGTCATAAAGGAAATGGCGAAATATGACAACATCAACTACGGaa > 2:395734/1‑136 (MQ=255) tCACCTATAGAACTTGAGAGGCCAGCAATGCAACCAGACTCGGTAATCTATCTAGATGCTGCAGCCACTACTCCGTGCGCTGATGACGTCATAAAGGAAATGGCGAAATATGACAACATCAACTACGGAAACCCCt < 1:529812/136‑1 (MQ=255) aGAACTTGAGAGGCCAGCAATGCAACCAGACTCGGTAATCTATCTAGATGCTGCAGCCACTACTCCGTGCGCTGATGACGTCATAAAGGAAATGGCGAAATATGACAACATCAACTACGGAAACCCCTCATCTgcg < 1:313905/136‑1 (MQ=255) gagGCCAGCAATGCAACCAGACTCGGTAATCTATCTAGATGCTGCAGCAACTACTCCGTGCGCTGATGACGTCATAAAGGAAATGGCGAAATATGACAACATCAACTACGGAAACCCCTCATCTGCGCACATTCTa > 1:530061/1‑136 (MQ=255) gagGCCAGCAATGCAACCAGACTCGGTAATCTATCTAGATGCTGCAGCAACTACTCCGTGCGCTGATGACGTCATAAAGGAAATGGCGAAATATGACAACATCAACTACGGAAACCCCTCATCTGCGCACATTCTa > 1:532582/1‑136 (MQ=255) agGCCAGCAATGCAACCAGACTCGGTAATCTATCTAGATGCTGCAGCAACTACTCCGTGCGCTGATGACGTCATAAAGGAAATGGCGAAATATGACAACATCAACTACGGAAACCCCTCATCTGCGCACATTCTAg < 2:532582/136‑1 (MQ=255) agGCCAGCAATGCAACCAGACTCGGTAATCTATCTAGATGCTGCAGCAACTACTCCGTGCGCTGATGACGTCATAAAGGAAATGGCGAAATATGACAACATCAACTACGGAAACCCCTCATCTGCGCACATTCTAg < 2:530061/136‑1 (MQ=255) tctatctaGATGCTGCAGCCACTACTCCGTGCGCTGATGACGTCATAAAGGAAATGGCGAAATATGACAACATCAACTACGGAAACCCCTCATCTGCGCACATTCTAGGCCGTCTTGCACGGAGTGCTGTCTTTGa < 1:116034/136‑1 (MQ=255) cAGCCACTACTCCGTGCGCTGATGACGTCATAAAGGAAATGGGGAAATATGACAACATCAACTACGGAAACCCCTCATCTGCGCACATTCTAGGCCGTCTTGCACGGAGTGCTGTCTTTGAAAGTGCTTCACatat > 2:433683/1‑136 (MQ=255) gCCACTACTCCGTGCGCTGATGACGTCATAAAGGAAATGGCGAAATATGACAACATCAACTACGGAAACCCCTCATCTGCGCACATTCTAGGCCGTCTTGCACGGAGTGCTGTCTTTGAAAGTGCTTCACATAttt < 1:433683/136‑1 (MQ=255) gCCACTACTCCGTGCGCTGATGACGTCATAAAGGAAATGGCGAAATATGACAACATCAACTACGGAAACCCCTCATCTGCGCACATTCTAGGCCGTCTTGCACGGAGTGCTGTCTTTGAAAGTGCTTCACATAttt > 1:184269/1‑136 (MQ=255) | GGCAGATCTGGGAGGGAGCGGGGAGCTCACGACCGAAGAAATATACAGCAAAGGCATCCATATCGTCACCTATAGAACTTGAGAGGCCAGCAATGCAACCAGACTCGGTAATCTATCTAGATGCTGCAGCCACTACTCCGTGCGCTGATGACGTCATAAAGGAAATGGCGAAATATGACAACATCAACTACGGAAACCCCTCATCTGCGCACATTCTAGGCCGTCTTGCACGGAGTGCTGTCTTTGAAAGTGCTTCACATATTT > NC_002947/4947468‑4947731 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |