Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,964,170 C→A 15.3% R432L (CGG→CTG)  fliF ← flagellar M‑ring protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,964,1700CA15.3% 77.5 / 2.2 26R432L (CGG→CTG) fliFflagellar M‑ring protein
Reads supporting (aligned to +/- strand):  ref base C (9/13);  new base A (2/2);  total (11/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-01

GGCACGTTGATCACCGTCACGCTGTCGCCACGGCTGGCATCGAAGCCGACTGCGTCCTGCACCAGGCGGGTAAAGCGCGCCAGGTCCTCGGCACCCCACGGTGCCCGCGTGGTATCGCCTGTGGCCGGGTCGATCTTGACCTGGTCGTCCACCACCACCGCTACCGACAGGCGGGCCATGCGCCCCTGCTGCTGGCGGGTGTGGCTGATGGAACGGTCCAGCTCGAAATTCTTGGT  >  NC_002947/4964065‑4964300
                                                                                                         |                                                                                                                                  
ggCACGTTGATCACCGTCACGCTGTCGCCACGGCTGGCATCGAAGCCGACTGCGTCCTGCACCAGGCGGGTAAAGCGCGCCAGGTCCTCGGCACCCCACGGTGCCCGCGTGGTATCGCCTGTGGCCGGGTCGATCt                                                                                                      >  1:24618/1‑136 (MQ=255)
ggCACGTTGATCACCGTCACGCTGTCGCCACGGCTGGCATCGAAGCCGACTGCGTCCTGCACCAGGCGGGTAAAGCGCGCCAGGTCCTCGGCACCCCACGGTGCCCGCGTGGTATCGCCTGTGGCCGGGTCGATCt                                                                                                      >  1:263879/1‑136 (MQ=255)
  cACGTTGATCACCGTCACGCTGTCGCCACGGCTGGCATCGAAGCCGACTGCGTCCTGCACCAGGCGGGTAAAGCGCGCCAGGTCCTCGGCACCCCACGGTGCCCGCGTGGTATCGCCTGTGGCCGGGTCGATCTTg                                                                                                    <  2:539313/136‑1 (MQ=255)
     gTTGATCACCGTCACGCTGTCGCCACGGCTGGCATCGAAGCCGACTGCGTCCTGCACCAGGCGGGTAAAGCGCGCCAGGTCCTCGGCACCCCACGGTGCCCGCGTGGTATCGCCTGTGGcc                                                                                                                >  2:452044/1‑121 (MQ=255)
     gTTGATCACCGTCACGCTGTCGCCACGGCTGGCATCGAAGCCGACTGCGTCCTGCACCAGGCGGGTAAAGCGCGCCAGGTCCTCGGCACCCCACGGTGCCCGCGTGGTATCGCCTGTGGcc                                                                                                                <  1:452044/121‑1 (MQ=255)
      ttGATCACCGTCACGCTGTCGCCACGGCTGGCATCGAAGCCGACTGCGTCCTGCACCAGGCGGGTAAAGCGCGCCAGGTCCTCGGCACCCCACGGTGCCCGCGTGGTATCGCCTGTGGCCGGGTCGATCTTGACCt                                                                                                <  1:93155/136‑1 (MQ=255)
               gTCACGCTGTCGCCACGGCTGGCATCGAAGCCGACTGCGTCCTGCACCAGGCGGGTAAAGCGCGCCAGGTCCTCGGCACCCCACGGTGCCCGCGTGGTATCGCCTGTGGCCGGGTCGATCTTGACCTGGTCGTcca                                                                                       >  2:9402/1‑136 (MQ=255)
               gTCACGCTGTCGCCACGGCTGGCATCGAAGCCGACTGCGTCCTGCACCAGGCGGGTAAAGCGCGCCAGGTCCTCGGCACCCCACGGTGCCCGCGTGGTATCGCCTGTGGCCGGGTCGATCTTGACCTGGTCGTcca                                                                                       >  2:11981/1‑136 (MQ=255)
                tCACGCTGTCGCCACGGCTGGCATCGAAGCCGACTGCGTCCTGCACCAGGCGGGTAAAGCGCGCCAGGTCCTCGGCACCCCACGGTGCCCGCGTGGTATCGCCTGTGGCCGGGTCGATCTTGACCTGGTCGTccac                                                                                      <  2:429541/136‑1 (MQ=255)
                      tGTCGCCACGGCTGGCATCGAAGCCGACTGCGTCCTGCACCAGGCGGGTAAAGCGCGCCAGGTCCTCGGCACCCCACGGTGCCCGCGTGGTATCGCCTGTGGCCGGGTCGATCTTGACCTGGTCGTCcaccaccac                                                                                >  2:444871/1‑136 (MQ=255)
                          gCCACGGCTGGCATCGAAGCCGACTGCGTCCTGCACCAGGCGGGTAAAGCGCGCCAGGTCCTCGGCACCCCACGGTGCCCGCGTGGTATCGCCTGTGGCCGGGTCGATCTTGACCTGGTCGTCCACCACCACCGCt                                                                            <  2:259115/136‑1 (MQ=255)
                           ccACGGCTGGCATCGAAGCCGACTGCGTCCTGCACCAGGCGGGTAAAGCGCGCCAGGTCCTCGGCACCCCACGGTGCCCGCGTGGTATCGCCTGTGGCCGGGTCGATCTTGACCTGGTCGTccaccac                                                                                   >  1:411184/1‑128 (MQ=255)
                           ccACGGCTGGCATCGAAGCCGACTGCGTCCTGCACCAGGCGGGTAAAGCGCGCCAGGTCCTCGGCACCCCACGGTGCCCGCGTGGTATCGCCTGTGGCCGGGTCGATCTTGACCTGGTCGTccaccac                                                                                   <  2:411184/128‑1 (MQ=255)
                           ccACGGCTGGCATCGAAGCCGACTGCGTCCTGCACCAGGCGGGTAAAGCGCGCCAGGTCCTCGGCACCCCACGGTGCCCGCGTGGTATCGCCTGTGGCCGGGTCGATCTTGACCTGGTCGTCCACCACCACCGCTa                                                                           >  1:410608/1‑136 (MQ=255)
                                     cATCGAAGCCGACTGCGTCCTGCACCAGGCGGGTAAAGCGCGCCAGGTCCTCGGCACCCCACGGTGCCCGCGTGGTATCGCCTGTGGCCGGGTCGATCTTGACCTGGTCGTCCACCACCACCGCTACCGACAGGCg                                                                 >  1:36757/1‑136 (MQ=255)
                                         gAAGCCGACTGCGTCCTGCACCAGGCGGGTAAAGCGCGCCAGGTCCTCGGCACCCCACGGTGCCCGCGTGGTATCGCCTGTGGCCGGGTCGATCTTGACCTGGTCGTCCACCACCACCGCTACCGACAGGCGGGcc                                                             <  1:499577/136‑1 (MQ=255)
                                           aGCCGACTGCGTCCTGCACCAGGCGGGTAAAGCGCGCCAGGTCCTCGGCACCCCACGGTGCCCGCGTGGTATCGCCTGTGGCCGGGTCGATCTTGACCTGGTCGTCCACCACCACCGCTACCGACAGGCGGGCCAt                                                           <  1:425804/136‑1 (MQ=255)
                                                  tGCGTCCTGCACCAGGCGGGTAAAGCGCGCCAGGTCCTCGGCACCACACGGTGCCAGCGTGGTATCGCCTGTGGCCGGGTCGATCTTGACCTGGTCGTCCACAACCACCGCTACCGACAGGCGGGCCATGCGcccc                                                    >  1:425431/1‑136 (MQ=255)
                                                  tGCGTCATGCACCAGGCGGGTAAAGCGCGCCAGGTCCTCGGCACCACACGGTGCCAGCGTGGTATCGCCTGTGGCCGGGTCGATCTTGACCTGGTCGTCCACAACCACCGCTACCGACAGGCGGGCCATGCGcccc                                                    >  1:42935/1‑136 (MQ=255)
                                                       ccTGCACCAGGCGGGTAAAGCGCGCCAGGTCCTCGGCACCCCACGGTGCCCGCGTGGTATCGCCTGTGGCCGGGTCGATCTTGACCTGGTCGTCCACCACCACCGCTACCGACAGGCGGGCCATGCGCCCctgctg                                               <  2:410608/136‑1 (MQ=255)
                                                                                             ccccACGGTGCCCGCGTGGTATCGCCTGTGGCCGGGTCGATCTTGACCTGGTCGTCCACCACCACCGCTACCGACAGGCGGGCCATGCGCCCCTGCTGCTGGCGGGTGTGGCTGATGGAACGGTCCAGCTCGAAAt         <  1:11981/136‑1 (MQ=255)
                                                                                             ccccACGGTGCCCGCGTGGTATCGCCTGTGGCCGGGTCGATCTTGACCTGGTCGTCCACCACCACCGCTACCGACAGGCGGGCCATGCGCCCCTGCTGCTGGCGGGTGTGGCTGATGGAACGGTCCAGCTCGAAAt         <  1:9402/136‑1 (MQ=255)
                                                                                                  cGGTGCCAGCGTGGTATCGCCTGTGGCCGGGTCGATCTTGACCTGGTCGTCCACAACCACCGCTACCGACAGGCGGGCCATGCGCCCCTGCTGCTGGCGGGTGTGGCTGATGGAACGGTCCAGCTCGAAATTCTTg    <  2:42935/136‑1 (MQ=255)
                                                                                                  cGGTGCCAGCGTGGTATCGCCTGTGGCCGGGTCGATCTTGACCTGGTCGTCCACAACCACCGCTACCGACAGGCGGGCCATGCGCCCCTGCTGCTGGCGGGTGTGGCTGATGGAACGGTCCAGCTCGAAATTCTTg    <  2:425431/136‑1 (MQ=255)
                                                                                                    gTGCCCGCGTGGTATCGCCTGTGGCCGGGTCGATCTTGACCTGGTCGTCCACCACCACCGCTACCGACAGGCGGGCCATGCGCCCCTGCTGCTGGCGGGTGTGGCTGATGGAACGGTCCAGCTCGAAATTCTTGGt  <  1:129585/136‑1 (MQ=255)
                                                                                                    gTGCCCGCGTGGTATCGCCTGTGGCCGGGTCGATCTTGACCTGGTCGTCCACCACCACCGCTACCGACAGGCGGGCCATGCGCCCCTGCTGCTGGCGGGTGTGGCTGATGGAACGGTCCAGCTCGAAATTCTTGGt  <  1:425828/136‑1 (MQ=255)
                                                                                                         |                                                                                                                                  
GGCACGTTGATCACCGTCACGCTGTCGCCACGGCTGGCATCGAAGCCGACTGCGTCCTGCACCAGGCGGGTAAAGCGCGCCAGGTCCTCGGCACCCCACGGTGCCCGCGTGGTATCGCCTGTGGCCGGGTCGATCTTGACCTGGTCGTCCACCACCACCGCTACCGACAGGCGGGCCATGCGCCCCTGCTGCTGGCGGGTGTGGCTGATGGAACGGTCCAGCTCGAAATTCTTGGT  >  NC_002947/4964065‑4964300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑