Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,144,362 C→A 22.2% L153M (CTG→ATG)  PP_4524 → sodium‑solute symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,144,3620CA22.2% 61.2 / 6.0 27L153M (CTG→ATG) PP_4524sodium‑solute symporter
Reads supporting (aligned to +/- strand):  ref base C (12/9);  new base A (3/3);  total (15/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

GCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCGGCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGACCGATATCGTCCAGTTCGTGATCAAGACCGTCGGCCTGATGTTCATCCTGCTGCCAGTGT  >  NC_002947/5144231‑5144488
                                                                                                                                   |                                                                                                                              
gCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGAt                                                                                                                            >  1:281703/1‑136 (MQ=255)
 cTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATg                                                                                                                           >  1:322912/1‑136 (MQ=255)
      aCCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTcgg                                                                                                                      <  1:224102/136‑1 (MQ=255)
           aCCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTcggcggcg                                                                                                                 <  2:122441/136‑1 (MQ=255)
             cGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTcggcggcggg                                                                                                               >  2:480854/1‑135 (MQ=255)
             cGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTATGATGCTCggcggcggc                                                                                                               >  2:405629/1‑136 (MQ=255)
             cGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTATGATGCTCggcggcggc                                                                                                               >  2:195224/1‑136 (MQ=255)
             cGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTATGATGCTCggcggcggc                                                                                                               >  2:14135/1‑136 (MQ=255)
              gCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGgcggcggcg                                                                                                              >  1:92481/1‑136 (MQ=255)
               cACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGgcggcggcgg                                                                                                             >  2:462786/1‑135 (MQ=255)
               cACGCCAGGCCAGCGCCGTCATAATGCAGGCTTAAGCACAGATGATCGGGGTCACCTCCCACCTGGCCATGGCCACCGACCTGCAGCTGGAGCTGGACCTCCCGATCAGGGCGACACAGATGCTCGGCGGCGGCGt                                                                                                             >  1:338527/1‑136 (MQ=255)
                                             cTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCGGCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCAt                                                                               <  2:281703/136‑1 (MQ=255)
                                             cTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCGGCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCAt                                                                               <  2:18244/136‑1 (MQ=255)
                                             cTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTATGATGCTCGGCGGCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCAt                                                                               <  1:14135/136‑1 (MQ=255)
                                             cTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTATGATGCTCGGCGGCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCAt                                                                               <  1:405629/136‑1 (MQ=255)
                                             cTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTATGATGCTCGGCGGCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCAt                                                                               <  1:195224/136‑1 (MQ=255)
                                               tATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCGGCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCAtgt                                                                             >  1:288373/1‑136 (MQ=255)
                                                 tGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCGGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCGGCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTgg                                                                           <  1:454473/136‑1 (MQ=255)
                                                    aCTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCAGATGCTCGGCGGCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCg                                                                        >  1:331976/1‑136 (MQ=255)
                                                            tCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCGGCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCt                                                                <  1:462786/136‑1 (MQ=255)
                                                                     ccTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCGGCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGACCGatat                                                       >  1:314259/1‑136 (MQ=255)
                                                                            ccTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCGGCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGACCGATATCGTCCAg                                                <  1:92477/136‑1 (MQ=255)
                                                                             cTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCGGCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGACCGATATCGTCCAGt                                               >  1:116071/1‑136 (MQ=255)
                                                                                                           ctgGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCGGCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGTTCGCTAACCCTGACCGATATCGTCCAGTTCGTGATCAAGACCGTCGGCCTGATGTTCa                 <  2:322912/136‑1 (MQ=255)
                                                                                                                      gTTCTGGGCTTCTCTGATGCTCGGCGGCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGACCGATATCGTCCAGTTCGTGATCAAGACCGTCGGCCTGATGTTCATCCTGCTGCCa      >  2:401840/1‑136 (MQ=255)
                                                                                                                      gTTCTGGGCTTCTCTGATGCTCGGCGGCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGACCGATATCGTCCAGTTCGTGATCAAGACCGTCGGCCTGATGTTCATCCTGCTGCCa      >  2:3966/1‑136 (MQ=255)
                                                                                                                          tGGGCTTCTCTGATGCTCGGCGGCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTAGCTAACCCTGACCGATATCGTCCAGTTCGTGATCAAGACCGTCGGCCTGATGTTCATCCTGCTGCCAgtgt  <  2:92481/136‑1 (MQ=255)
                                                                                                                                   |                                                                                                                              
GCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATGCACTGATGATCGGGGTCACCTCGACCCTGGCCATGGCCACCGTCCTGCAGGTGCTGCTGGACCTGCCGTTCTGGGCTTCTCTGATGCTCGGCGGCGGCGTGGTAGTGCTGTACTCGACCATCGGCGGCATGTGGTCGCTAACCCTGACCGATATCGTCCAGTTCGTGATCAAGACCGTCGGCCTGATGTTCATCCTGCTGCCAGTGT  >  NC_002947/5144231‑5144488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑