| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 212,038 | C→A | 26.7% | V5848V (GTC→GTA) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 212,038 | 0 | C | A | 26.7% | 22.3 / 3.1 | 15 | V5848V (GTC→GTA) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base C (6/5); new base A (2/2); total (8/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.46e-01 | |||||||||||
GACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGT > NC_002947/211904‑212155 | gacgaGCCAGGTACGCCGGGCAACGAACGCGCACTGATCAAGGTCACCATCCCCCCCGTCCAGACTTCCGTAGCCGAGAACGTCACACCGCCCTTCAATGTTCACTTCAACCAGCCGCTGGCTCGCGCGCTGGTCg > 1:28778/1‑136 (MQ=11) gaGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTgg > 2:7016/1‑135 (MQ=21) gCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGAcc < 2:194480/136‑1 (MQ=34) gCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGAcc < 2:382394/136‑1 (MQ=34) agaACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCGCAggg > 1:335295/1‑136 (MQ=32) agaACGTCAAACCGACCTTCACTGTTCACGTAAACCAGCCGCTGGCTCACGACCTGGTAGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCGCAggg > 1:129894/1‑136 (MQ=17) agaACGTCAAACCGACCTTCACTGTTCACGTAAACCAGCCGCTGGCTCACGACCTGGTAGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCGCAggg > 1:333439/1‑136 (MQ=17) gaACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGt < 2:259210/136‑1 (MQ=32) gaACGTCAAACCGACCTTCACTGTTCACGTAAACCAGCCGCTGGCTCACGACCTGGTAGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGt < 2:129894/136‑1 (MQ=17) gaACGTCAAACCGACCTTCACTGTTCACGTAAACCAGCCGCTGGCTCACGACCTGGTAGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGt < 2:333439/136‑1 (MQ=17) aaCGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCCGCGCGCCGTACACCCACGCTGCGCAGGGtg > 2:425748/1‑136 (MQ=17) aaCGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATACCCAGGTCACCATCAAGGCCCGTGAAACCAGCGCGCCGTACACCCACGCAGCGCACGGtg > 2:379480/1‑136 (MQ=255) cAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGAc < 1:464115/136‑1 (MQ=32) cGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGATGCTGGCCAg < 1:379480/136‑1 (MQ=34) gCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGt > 2:292977/1‑136 (MQ=34) | GACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGT > NC_002947/211904‑212155 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |