| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,785,269 | G→T | 20.0% | D190Y (GAC→TAC) | cvrA → | K(+)/H(+) antiporter NhaP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,785,269 | 0 | G | T | 20.0% | 50.7 / 2.6 | 20 | D190Y (GAC→TAC) | cvrA | K(+)/H(+) antiporter NhaP |
| Reads supporting (aligned to +/- strand): ref base G (9/7); new base T (2/2); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
AGTGTTCTCGCTGCTCGGCGGCAAAGGCCTGAACGAGCGGGTTTCCGCTACCCTCGAGATCGAGTCGGGCAGCAACGACCCGATGGCAGTGTTCCTCACTGTCACCTTGATCGACATGATCGCCAGCGGCGAGACCGGCCTGCACTGGAGCCTGCTGGGCCACTTGCTGCGTGAGTTCGGTATCGGCACGCTGCTGGGCCTGGGCGGCGGCTGGCTGATGCTGCAACTGGTAAACCGTATCA > NC_002947/5785157‑5785398 | aGTGTTCTCGCTGCTCGGCGGCAAAGGCCTGAACGAGCGGGTTTCCGCTACCCTCGAGATCGAGTCGGGCAGCAACGACCCGATGGCAGTGTTCCTCACTGTCACCTTGATCGACATGATCGCCAGCGGCGAGAcc < 2:372530/136‑1 (MQ=255) gggtTCTCGCTGCTCGGCGGCAAAGGCCTGAACGAGCGGGGTTCCGCTACCCTCGAGATCGAGTCGGGCTGCAACGACCCGATGGCAGTGTTCCTCACTGTCACGTTGATCGACATGATCGCCAGCGGCGAGAGCg < 2:260346/134‑1 (MQ=255) tcGCTGCTCGGCGGCAAAGGCCTGAACTAGCGGGTTTCCGCTACCCTCGAGATCGAGTCGGGCAGCAACGACCCGATGGCAGTGTTCCTCACTGTCACCTTGATCTACATGATCGCCAGCGGCGAGACCGGCCTGc > 2:397205/1‑136 (MQ=255) tcGCTGCTCGGCGGCAAAGGCCTGAACGAGCGGGTTTCCGCTACCCTCGAGATCGAGTCGGGCAGCAACGACCCGATGGCAGTGTTCCTCACTGTCACCTTGATCTACATGATCGCCAGCGGCGAGACCGGCCTGc > 2:305624/1‑136 (MQ=255) tcGCTGCTCGGCGGCAAAGGCCTGAACGAGCGGGTTTCCGCTACCCTCGAGATCGAGTCGGGCAGCAACGACCCGATGGCAGTGTTCCTCACTGTCACCTTGATCGACATGATCGCCAGCGGCGAGACCGGCCTGc > 2:108029/1‑136 (MQ=255) aaaaGGCCTGAACGAGCGGGTTTACGCTACCCTCGAGATCGAGTCGGGCAGCAACGACCAGATGGCAGTGTTCCTCACTGTCACCTTGATCGACATGATCGCCAGCGGCGAGACCGGCCTGCACTGGAGCctgctg < 1:87692/135‑1 (MQ=255) gCCTGAACGAGCGGGTTTCCGCTACCCTCGAGATCGAGTCGGGCAGCAACGACCCGATGGCAGTGTTCCTCACTGTCACCTTGATCGACATGATCGCCAGCGGCGAGACCGGCCTGCACTGGAGCCTGCTGGGCCa > 2:275671/1‑136 (MQ=255) gAGCGGGTTTCCGCTACCCTCGAGATCGAGTCGGGCAGCAACGACCCGATGGCAGTGTTCCTCACTGTCACCTTGATCGACATGATCGCCAGCGGCGAGACCGGCCTGCACTGGAGCCTGCTGGGCCACTtgctgc > 1:272746/1‑136 (MQ=255) gtgTTTCCGCTACCCTCGAGATCGAGTCGGTCAGCAACGACCCGATGGCAGTGTTGCTCACTGTCACCTTGATCGACATGATCGCCAGCGGCGAGACCGGCCTGCACTGGAGCCTGCTGGGCCACTTGCTGCGTGa < 2:387219/134‑1 (MQ=255) ggTTTCCGCTACCCTCGAGATCGAGTCGGGCAGCAACGACCCGATGGCAGTGTTCCTCACTGTCACCTTGATCGACATGATCGCCAGCGGCGAGACCGGCCTGCACTGGAGCCTGCTGGGCCACTTGCTGCGTGAg > 1:321116/1‑136 (MQ=255) ggTTTCCGCTACCCTCGAGATCGAGTCGGGCAGCAACGACCCGATGGCAGTGTTCCTCACTGTCACCTTGATCGACATGATCGCCAGCGGCGAGACCGGCCTGCACTGGAGCCTGCTGGGCCACTTGCTGCGTGAg > 1:195861/1‑136 (MQ=255) ggTTTCCGCTACCCTCGAGATCGAGTCGGGCAGCAACGACCCGATGGCAGTGTTCCTCACTGTCACCTTGATCGACATGATCGCCAGCGGCGAGACCGGCCTGCACTGGAGCCTGCTGGGCCACTTGCTGCGTGAg > 1:193911/1‑136 (MQ=255) gagaTCGAGTCGGGCAGCAACGACCCGATGGCAGTGTTCCTCACTGTCACCTTGATCGACTTGATCGCCAGCGGCGAGACCGGCCTGCACTGGAGCCTGCTGGGCCACTTGCTGCGTGAGTTCGGTATCGGCACgc < 2:123911/136‑1 (MQ=255) agcaACGACCCGATGGCAGTGTTCCTCACTGTCACCTTGATCGACATGATCGCCAGCGGCGAGACCGGCCTGCACTGGAGCCTGCTGGGCCACTTGCTGCGTGAGTTCGGTATCGGCACGCTGCTGGGCCTGggcg > 2:196856/1‑136 (MQ=255) gCAGTGTTCCTCACTGTCACCTTGATCGACATGATCGCCAGCGGCGAGACCGGCCTGCACTGGAGCCTGCTGGGCCACTTGCTGCGTGAGTTCGGTATCGGCACGCTGCTGGGCCTTGGCGGCGGCTGGCTGAtgc < 1:108029/136‑1 (MQ=255) gCAGTGTTCCTCACTGTCACCTTGATCGACATGATCGCCAGCGGCGAGACCGGCCTGCACTGGAGCCTGCTGGGCCACTTGCTGCGTGAGTTCGGTATCGGCACGCTGCTGGGCCTGGGCGGCGGCTGGCTGAtgc < 1:211326/136‑1 (MQ=255) tCACTGTCACCTTGATCTACATGATCGCCAGCGGCGAGACCGGCCTGCACTGGAGCCTGCTGGGCCACTTGCTGCGTGAGTTCGGTATCGGCACGCTGCTGGGCCTGGGCGGCGGCTGGCTGATGCTGCAACTGGt < 1:397205/136‑1 (MQ=255) tCACTGTCACCTTGATCTACATGATCGCCAGCGGCGAGACCGGCCTGCACTGGAGCCTGCTGGGCCACTTGCTGCGTGAGTTCGGTATCGGCACGCTGCTGGGCCTGGGCGGCGGCTGGCTGATGCTGCAACTGGt < 1:305624/136‑1 (MQ=255) cACTGTCACCTTGATCGACATGATCGCCAGCGGCGAGACCGGCCTGCACTGGAGCCTGCTGGGCCACTTGCTGCGTGAGTTCGGTATCGGCACGCTGCTGGGCCTGGGCGGCGGCTGGCTGATGCTGCAACTCGTa > 1:339392/1‑136 (MQ=255) ttGATCGACATGATCGCCAGCGGCGAGACCGGCCTGCACTGGAGCCTGCTGGGCCACTTGCTGCGTGAGTTCGGTATCGGCACGCTGCTGGGCCTGGGCGGCGGCTGGCTGATGCTGCAACTGGTAAACCGTATCa > 1:424684/1‑136 (MQ=255) | AGTGTTCTCGCTGCTCGGCGGCAAAGGCCTGAACGAGCGGGTTTCCGCTACCCTCGAGATCGAGTCGGGCAGCAACGACCCGATGGCAGTGTTCCTCACTGTCACCTTGATCGACATGATCGCCAGCGGCGAGACCGGCCTGCACTGGAGCCTGCTGGGCCACTTGCTGCGTGAGTTCGGTATCGGCACGCTGCTGGGCCTGGGCGGCGGCTGGCTGATGCTGCAACTGGTAAACCGTATCA > NC_002947/5785157‑5785398 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |