| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,990,934 | G→T | 21.7% | D66Y (GAC→TAC) | PP_5245 → | AraC family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,990,934 | 0 | G | T | 21.7% | 56.2 / 4.8 | 23 | D66Y (GAC→TAC) | PP_5245 | AraC family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (9/9); new base T (2/3); total (11/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-01 | |||||||||||
GATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTGCAGCACGGCAATATCGGCTGCTGACCTTGGCCGAGGGGCAAGCAGTGGTACGCGGCTCATGCGGCCTGAAGGTAGTGGCCGACCGGCGCCTGGAAGACTTGCCGGAGGCCGTCGACCTGTTGCTGGTGCCGGGCGGCCCTGGGGCCTATGACATCGCCCTGCCAGGGATCGAGCGCTGGCTGCCCAAGGCCGTGCGCCAG > NC_002947/5990802‑5991053 | gATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTGCAGCACGGCAATATCGGCTGCTGACCTTGGCCGAGGGGCAAGCAGTGGTACGCGGCTCATGCGGCCTGAAGGTAGTGGCCGAcc < 2:370638/136‑1 (MQ=255) tGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTGCAGCACGGCAATATCGGCTGCTGACCTTGGCCGAGGGGCAAGCAGTGGTACGCGGCTCATGCGGCCTGAAGGTAGTGGCCGACCgg > 1:42826/1‑136 (MQ=255) cGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTGCAGCACGGCAATATCGGCTGCTGACCTTGGCCGAGGGGCAAGCAGTGGTACGCGGCTCATGCGGCCTGAAGGTAGTGGCCGACCGGCGCCTg > 1:436865/1‑136 (MQ=255) caACCGGTTCTTGCCTGCAGCACGGCAATATCGGCTGCTGACCTTGGCCGAGGGGCAAGCAGTGGTACGCGGCTCATGCGGCCTGAAGGTAGTGGCCGACCGGCGCCTGGAAGACTTGCCGGAGGCCGTCGACCTg > 1:275068/1‑136 (MQ=255) gTTCTTGCCTGCAGCACGGCAATATCGGCTGCTGACCTTGGCCGAGGGGCAAGCAGTGGTACGCGGCTCATGCGGCCTGAAGGTAGTGGCCGACCGGCGCCTGGAAGACTTGCCGGAGGCCGTCGACCTGTTGCTg < 1:76945/136‑1 (MQ=255) tGCCTGCAGCACGGCAATATCGGCTGCTGACCTTGGCCGAGGGGCAAGCAGTGGTACGCGGCTCATGCGGCCTGAAGGTAGTGGCCGACCGGCGCCTGGAAGACTTGCCGGAGGCCGTCGACCTGTTGCTGGTGcc < 2:436865/136‑1 (MQ=255) tGCCTGCAGCACGGCAATATCGGCTGCTGACCTTGGCCGAGGGGCAAGCAGTGGTACGCGGCTCATGCGGCCTGAAGGTAGTGGCCGACCGGCGCCTGGAAGACTTGCCGGAGGCCGTCGACCTGTTGCTGGTGcc < 1:527658/136‑1 (MQ=255) gcaCGGCAATATCGGCTGCTGACCTTGGCCGAGGGGCAAGCAGTGGTACGCGGCTCATGCGGCCTGAAGGTAGTGGCCGTCCGGCGCCTGGAAGACTTGCCGGAGGCCGTCGACCTGTTGCTGGTGCCGGGCGGcc < 1:471716/136‑1 (MQ=255) ccaCGGCAATATCGGCTGCTGACCTTGGCCGAGGGGCAAGCAGTGGTACGCGGCTCATGCGGCCTGAAGGTAGTGGCCGACCGGCGCCTGGAAGACTTGCCGGAGGCCGTCGACCTGTTGCTGGTGCCGGGCGGcc < 1:517309/135‑1 (MQ=255) caCGGCAATATCGGCTGCTGACCTTGGCCGAGGGGCAAGCAGTGGTACGCGGCTCATGCGGCCTGAAGGTAGTGGCCGACTGGCGCCTGGAAGACTTGCCGGAGGCCGTCGACCTGTTGCTGGTGCCGGGCGGccc > 1:85879/1‑136 (MQ=255) caCGGCAATATCGGCTGCTGACCTTGGCCGAGGGGCAAGCAGTGGTACGCGGCTCATGCGGCCTGAAGGTAGTGGCCGACCGGCGCCTGGAAGACTTGCCGGAGGCCGTCGACCTGTTGCTGGTGCCGGGCGGccc > 1:295414/1‑136 (MQ=255) gctgctGACCTTGGCCGAGGGGCAAGCAGTGGTACGCGGCTCATGCGGCCTGAAGGTAGTGGCCTACCGGCGCCTGGAAGACTTGCCGGAGGCCGTCGACCTGTTGCTGGTGCCGGGCGGCCCTGGGGCCTATGAc > 2:476157/1‑136 (MQ=255) gctgctGACCTTGGCCGAGGGGCAAGCAGTGGTACGCGGCTCATGCGGCCTGAAGGTAGTGGCCTACCGGCGCCTGGAAGACTTGCCGGAGGCCGTCGACCTGTTGCTGGTGCCGGGCGGCCCTGGGGCCTATGAc > 2:470431/1‑136 (MQ=255) gctgACCTTGGCCGAGGGGCAAGCAGTGGTACGCGGCTCATGCGGCCTGAAGGTAGTGGCCGACCGGCGCCTGGAAGACTTGCCGGAGGCCGTCGACCTGTTGCTGGTGCCGGGCGGCCCTGGGGCCTATGACATc < 1:115484/136‑1 (MQ=255) ccTTGGCCGAGGGGCAAGCAGTGGTACGCGGCTCATGCGGCCTGAAGGTAGTGGCCTACCGGCGCCTGGAAGACTTGCCGGAGGCCGTCGACCTGTTGCTGGTGCCGGGCGGCCCTGGGGCCTATGACATCGCCCt < 1:151117/136‑1 (MQ=255) ttGGCCGAGGGGCAAGCAGTGGTACGCGGCTCATGCGGCCTGAAGGTAGTGGCCGACCGGCGCCTGGAAGACTTGCCTGAGGCCGTCGACCTGTTGCTGGTGCCGGGCGGCCCTGGGGCCTATGACATCGCCCTGc < 2:37431/136‑1 (MQ=255) cGAGGGGCAAGCAGTGGTACGCGGCTCATGCGGCCTGAAGGTAGTGGCCGACCGGCGCCTGGAAGACTTGCCGGAGGCCGTCGACCTGTTGCTGGTGCCGGGCGGCCCTGGGGCCTATGACATCGCCCTGCCAggg > 2:497888/1‑136 (MQ=255) cgGCTCATGCGGCCTGAAGGTAGTGGCCTACCGGCGCCTGGAAGACTTGCCGGAGGCCGTCGACCTGTTGCTGGTGCCGGGCGGCCCTGGGGCCTATGACATCGCCCTGCCAGGGATCGAGCGCTGGCTGCCCAAg < 1:476157/136‑1 (MQ=255) cgGCTCATGCGGCCTGAAGGTAGTGGCCTACCGGCGCCTGGAAGACTTGCCGGAGGCCGTCGACCTGTTGCTGGTGCCGGGCGGCCCTGGGGCCTATGACATCGCCCTGCCAGGGATCGAGCGCTGGCTGCCCAAg < 1:470431/136‑1 (MQ=255) ggCTCATGCGGCCTGAAGGTAGTGGCCGACCGGCGCCTGGAAGACTTGCCGGAGGCCGTCGACCTGTTGCTGGTGCCGGGCGGCCCTGGGGCCTATGACATCGCCCTGCCAGGGATCGAGCGCTGGCTGCCCAAgg > 1:465857/1‑136 (MQ=255) aTGCGGCCTGAAGGTAGTGGCCGACATTAGCCTGGAAGACTTGCCGGAGGCCGTCGACCTGTTGCTGGTGCCGGGCGGCCCTGGGGCCTATGACATCGCCCTGCCAGGGATCGAGCGCTGGCTGCCCAAGGCCGTg > 2:278771/1‑136 (MQ=255) aTGCGGCCTGAAGGTAGTGGCCGACAAGAGCCTGGAAGACTTGCCGGAGGCCGTCGACCTGTTGCTGGTGCCGGGCGGCCCTGGGGCCTATGACATCGCCCTGCCAGGGATCGAGCGCTGGCTGCCCAAGGCCGTg > 2:314006/1‑136 (MQ=255) ccTGAAGGTAGTGGCCGACTGGCGCCTGGAAGACTTGCCGGAGGCCGTCGACCTGTTGCTGGTGCCGGGCGGCCCTGGGGCCTATGACATCGCCCTGCCAGGGATCGAGCGCTGGCTGCCCAAGGCCGTGCGCCAg < 2:85879/136‑1 (MQ=255) | GATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTGCAGCACGGCAATATCGGCTGCTGACCTTGGCCGAGGGGCAAGCAGTGGTACGCGGCTCATGCGGCCTGAAGGTAGTGGCCGACCGGCGCCTGGAAGACTTGCCGGAGGCCGTCGACCTGTTGCTGGTGCCGGGCGGCCCTGGGGCCTATGACATCGCCCTGCCAGGGATCGAGCGCTGGCTGCCCAAGGCCGTGCGCCAG > NC_002947/5990802‑5991053 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |