| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,078,315 | G→T | 19.9% | L316M (CTG→ATG) | PP_5327 ← | phosphate ABC transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,078,315 | 0 | G | T | 19.9% | 54.8 / 2.6 | 20 | L316M (CTG→ATG) | PP_5327 | phosphate ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base G (10/6); new base T (2/2); total (12/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
GCAGGAACACCGGCCAGGTTGTTAACCGCAATGCGGATAAGCCGGGTGACCGGGCCCTGCCTGGCGTATTCACGCAGGTACACGGCAGCCAGCACGCCGAACGGGGTAACGATCACGGCCATGATCAGGGTCATCATCACGGTACCGAAGATGGCCGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACA > NC_002947/6078188‑6078440 | gCAGGAACACCGGCCAGGTTGTTAACCGCAATGCGGATAAGCCGGGTGACCGGGCCCTGCCTGGCGTATTCACGCAGGTACACGGCAGCCAGCACGCCGAACGGGGTAACGATCACGGCCATGATCAGGGtcatca > 1:36453/1‑136 (MQ=255)gCAGGAACACCGGCCAGGTTGTTAACCGCAATGCGGATAAGCCGGGTGACCGGGCCCTGCCTGGCGTATTCACGCAGGTACACGGCAGCCAGCACGCCGAACGGGGTAACGATCACGGCCATGATCAGGGtcatca < 1:124249/136‑1 (MQ=255)gCAGGAACACCGGCCAGGTTGTTAACCGCAATGCGGATAAGCCGGGTGACCGGGCCCTGCCTGGCGTATTCACGCAGGTACACGGCAGCCAGCACGCCGAACGGGGTAACGATCACGGCCATGATCAGGGtcatca < 1:475204/136‑1 (MQ=255) gCCAGGTTGTTAACCGCAATGCGGATAAGCCGGGTGACCGGGCCCTGCCTGGCGTATTCACGCAGGTACACGGCAGCCAGCACGCCGAACGGGGTAACGATCACGGCCATGATCAGGGTCATCATCACGGTACCGa < 2:36453/136‑1 (MQ=255) ccAGGTTGTTAACCGCAATGCGGATACGCCGGGTGACCGGGCCCTGCCTGGCGTATTCACGCAGGTACACGGCAGCCAGCACGCCGAACGGGGTAACGATCACGGCCATGATCAGGGTCATCATCACGGTACCGaa > 2:392830/1‑136 (MQ=255) aGGTTGTTAACCGCAATGCGGATAAGCCGGGTGACCGGGCCCTGCCTGGCGTATTCACGCAGGTACACGGCAGCCAGCACGCCGAACGGGGTAACGATCACGGCCATGATCAGGGTCATCATCACGGTACCGAAGa > 2:387206/1‑136 (MQ=255) cGGATAAGCCGGGTGACCGGGCCCTGCCTGGCGTATTCACGCAGGTACACGGCAGCCAGCACGCCGAACGGGGTAACGATCACGGCCATGATCAGGGTCATCATCACGGTACCGAAGATGGCCGGGAAGATACCGc < 1:298838/136‑1 (MQ=255) cGGGTGACCGGGCCCTGCCTGGCGTATTCACGCAGGTACACGGCAGCCAGCACGCCGAACGGGGTAACGATCACGGCCATGATCATGGTCATCATCACGGTACCGAAGATGGCCGGGAAGATACCGCCCTCGgtgt > 2:464588/1‑136 (MQ=255) cGGGTGACCGGGCCCTGCCTGGCGTATTCACGCAGGTACACGGCAGCCAGCACGCCGAACGGGGTAACGATCACGGCCATGATCATGGTCATCATCACGGTACCGAAGATGGCCGGGAAGATACCGCCCTCGgtgt > 2:19312/1‑136 (MQ=255) tGACCGGGCCCTGCCTGGCGTATTCACGCAGGTACACGGCAGCCAGCACGCCGAACGGGGTAACGATCACGGCCATGATCAGGGTCATCATCACGGTACCGAAGATGGCCGGGAAGATACCGCCCTCGGTGTTGGc > 2:517137/1‑136 (MQ=255) aCCGGGCCCTGCCTGGCGTATTCACGCAGGTACACGGCAGCCAGCACGCCGAACGGGGTAACGATCACGGCCATGATCAGGGTCATCATCACGGTACCGAAGATGGCCGGGAAGATACCGCCCTCGGTGTTGGCtt > 1:212514/1‑136 (MQ=255) gCCCTGCCTGGCGTATTCACGCAGGTACACGGCAGCCAGCACGCCGAACGGGGTAACGATCACGGCCATGATCAGGGTCATCATCACGGTACCGAAGATGGCCGGGAAGATACCGCCCTCGGTGTTGGCTTCAcgc < 2:459321/136‑1 (MQ=255) gTATTCACGCAGGTACACGGCAGCCAGCACGCCGAACGGGGTAACGATCACGGCCATGATCATGGTCATCATCACGGTACCGAAGATGGCCGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTg < 1:464588/136‑1 (MQ=255) gTATTCACGCAGGTACACGGCAGCCAGCACGCCGAACGGGGTAACGATCACGGCCATGATCATGGTCATCATCACGGTACCGAAGATGGCCGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTg < 1:19312/136‑1 (MQ=255) aTTCACGCAGGTACACGGCAGCCAGCACGCCGAACGGGGTAACGATCACGGCCATGATCAGGGTCATCATCACGGTACCGAAGATGGCCGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAg > 1:409143/1‑136 (MQ=255) aTTCACGCAGGTACACGGCAGCCAGCACGCCGAACGGGGTAACGATCACGGCCATGATCAGGGTCATCATCACGGTACCGAAGATGGCCGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAg > 1:40847/1‑136 (MQ=255) aCGCAGGTACACGGCAGCCAGCACGCCGAACGGGGTAACGATCACGGCCATGATCAGGGTCATCATCACGGTACCGAAGATGGCCGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAGGAAc > 2:6796/1‑136 (MQ=255) gAACGGGGTAACGATCACGGCCATGATCAGGGTCATCATCACGGTACCGAAGATGGCCGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTg < 1:505671/103‑1 (MQ=255) gAACGGGGTAACGATCACGGCCATGATCAGGGTCATCATCACGGTACCGAAGATGGCCGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTg > 2:505671/1‑103 (MQ=255) gCCATGATCAGGGTCATCATCACGGTACCGAAGATGGCCGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACa > 1:178510/1‑136 (MQ=255) | GCAGGAACACCGGCCAGGTTGTTAACCGCAATGCGGATAAGCCGGGTGACCGGGCCCTGCCTGGCGTATTCACGCAGGTACACGGCAGCCAGCACGCCGAACGGGGTAACGATCACGGCCATGATCAGGGTCATCATCACGGTACCGAAGATGGCCGGGAAGATACCGCCCTCGGTGTTGGCTTCACGCGGGTCGTCGCTGAGGAACTCCCAGACCTTGCCGAAGTAGGTGCCCAACTTGGTGAACGCCGACA > NC_002947/6078188‑6078440 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |