| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 289,318 | G→T | 16.6% | D246Y (GAC→TAC) | oprE → | outer‑membrane porin E |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 289,318 | 0 | G | T | 16.6% | 73.6 / 2.2 | 24 | D246Y (GAC→TAC) | oprE | outer‑membrane porin E |
| Reads supporting (aligned to +/- strand): ref base G (12/8); new base T (2/2); total (14/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTGATTACAAGCTCACCCCAGACCTGACTCTGCAGTACTACTACTCGAACCTGGAAGACTTCTACAAGCAGCACTTCCTGGGTGCGACCCACGTATTCAAGATTGCCGACAACCAGTCGTTCAAGACTGACCTGCGCTACTTCGACAGCAGCAGCGACGGCAAGAATGGCCAGACTGGCTATGCCTTCGACAACAACGGTGGCTACGCGAAGAACTCGGGCGAGGTCGACAACAAGACCTGGAGCGCCATGTTCACCTAC > NC_002947/289189‑289449 | ggTGATTACAAGCTCACCCCAGACCTGACTCTGCAGTACTACTACTCGAACCTGGAAGACTTCTACAAGCAGCACTTCCTGGGTGCGACCCACGTATTCAAGATTGCCGACAACCAGTCGTTCAAGACTGACCTgc < 1:172110/136‑1 (MQ=255) cTCACCCCAGACCTGACTCTGCAGTACTACTACTCGAACCTGGAAGACTTCTACAAGCAGCACTTCCTGGGTGCGACCCACGTATTCAAGATTGCCGACAACCAGTCGTTCAAGACTGACCTGCGCTACTTCGAca > 2:253091/1‑136 (MQ=255) aCCCCAGACCTGACTCTGCAGTACTACTACTCGAACCTGGAAGACTTCTACAAGCAGCACTTCCTGGGTGCGACCCACGTATTCAAGATTGCCGACAACCAGTCGTTCAAGACTGACCTGCGCTACTTCGAcagca > 1:158525/1‑136 (MQ=255) cccAGACCTGACTCTGCAGTACTACTACTCGAACCTGGAAGACTTCTACAAGCAGCACTTCCTGTGTGCGACCCACGTATTCAAGATTGCCGACAACCAGTCGTTCAAGACTTACCTGCGCt < 1:35075/122‑1 (MQ=255) cccAGACCTGACTCTGCAGTACTACTACTCGAACCTGGAAGACTTATACAAGCAGCACTTCCTGTGTGCGACCCACGTATTCAAGATTGCCGACAACCAGTCGTTCAAGACTTACCTGCGCt > 2:35075/1‑122 (MQ=255) tGACTCTGCAGTACTACTACTCGAACCTGGAAGACTTCTACAAGCAGCACTTCCTGGGTGCGACCCACGTATTCAAGATTGCCGACAACCAGTCGTTCAAGACTGACCTGCGCTACTTCGACAGCAGCAGCGACgg > 1:144730/1‑136 (MQ=255) ctGCAGTACTACTACTCGAACCTGGAAGACTTCTACAAGCAGCACTTCCTGGGTGCGACCCACGTATTCAAGATTGCCGACAACCAGTCGTTCAAGACTGACCTGCGCTACTTCGACAGCAGCAGCGACGGCAAGa > 2:104042/1‑136 (MQ=255) tactactacTCGAACCTGGAAGACTTCTACAAGCAGCACTTCCTGGGTGCGACCCACGTATTCAAGATTGCCGACAACCAGTCGTTCAAGACTGACCTGCGCTACTTCGACAGCAGCAGCGACGGCAAGAATGGcc < 1:524593/136‑1 (MQ=255) ctCGAACCTGGAAGACTTCTACAAGCAGCACTTCCTGGGTGCGACCCACGTATTCAAGATTGCCGACAACCAGTCGTTCAAGACTTACCTGCGCTACTTCGACAGCAGCAGCGACGGCAAGAATGGCCAGACTGGc > 1:87728/1‑136 (MQ=255) gAAGACTTCTACAAGCAGCACTTCCTGGGTGCGACCCACGTATTCAAGATTGCCGACAACCAGTCGTTCAAGACTGACCTGCGCTACTTCGACAGCAGCAGCGACGGCAAGAATGGCCAGACTGGCTATGCCTTCg > 2:478395/1‑136 (MQ=255) gAAGACTTCTACAAGCAGCACTTCCTGGGTGCGACCCACGTATTCAAGATTGCCGACAACCAGTCGTTCAAGACTGACCTGCGCTACTTCGACAGCAGCAGCGACGGCAAGAATGGCCAGACTGGCTATGCCTTCg > 2:459733/1‑136 (MQ=255) gACTTCTACAAGCAGCACTTCCTGGGTGCGACCCACGTATTCAAGATTGCCGACAACCAGTCGTTCAAGACTGACCTGCGCTACTTCGACAGCAGCAGCGACGGCAAGAATGGCCAGACTGGCTATGCCTTCGaca > 2:279692/1‑136 (MQ=255) aCTTCCTGGGTGCGACCCACGTATTCAAGATTGCCGACAACCAGTCGTTCAAGACTGACCTGCGCTACTTCGACAGCAGCAGCGACGGCAAGAATGGCCAGACTGGCTATGCCTTCGACAACAACGGTGGCTAcgc > 2:139751/1‑136 (MQ=255) aCCCACGTATTCAAGATTGCCGACAACCAGTCGTTCAAGACTTACCTGCGCTACTTCGACAGCAGCAGCGACGGCAAGAATGGCCAGACTGGCTATGCCTTCGACAACAACGGTGGCTACGCGAAGAACTCGGGCg < 2:87728/136‑1 (MQ=255) cccACGTATTCAAGATTGCCGACAACCAGTCGTTCAAGACTGACCTGCGCTACTTCGACAGCAGCAGCGACGGCAAGAATGGCCAGACTGGCTATGCCTTCGACAACAACGGTGGCTACGCGAAGAACTCGGGCGa > 2:29655/1‑136 (MQ=255) cACGTATTCAAGATTGCCGACAACCAGTCGTTCAAGACTGACCTGCGCTACTTCGACAGCAGCAGCGACGGCAAGAATGGCCAGACTGGCTATGCCTTCGACAACAACGGTGGCTACGCGAAGAACTCGGGCGAgg > 2:114303/1‑136 (MQ=255) cACGTATTCAAGATTGCCGACAACCAGTCGTTCAAGACTGACCTGCGCTACTTCGACAGCAGCAGCGACGGCAAGAATGGCCAGACTGGCTATGCCTTCGACAACAACGGTGGCTACGCGAAGAACTCGGGCGAgg > 1:286369/1‑136 (MQ=255) aTTCAAGATTGCCGACAACCAGTCGTTCAAGACTGACCTGCGCTACTTCGACAGCAGCAGCGACGGCAAGAATGGCCAGACTGGCTATGCCTTCGACAACAACGGTGGCTACGCGAAGAACTCGGGCGAGGTCGac < 1:237860/136‑1 (MQ=255) gATTGCCGACAACCAGTCGTTCAAGACTGACCTGCGCTACTTCGACAGCAGCAGCGACGGCAAGAATGGCCAGACTGGCTATGCCTTCGACAACAACGGTGGCTACGCGAAGAACTCGGGCGAGGTCGACAACAAg > 1:165827/1‑136 (MQ=255) gCCGACAACCAGTCGTTCAAGACTGACCTGCGCTACTTCGACAGCAGCAGCGACGGCAAGAATGGCCAGACTGGCTATGCCTTCGACAACAACGGTGGCTACGCGAAGAACTCGGGCGAGGTCGACAACAAGACCt < 1:64897/136‑1 (MQ=255) aGTCGTTCAAGACTGACCTGCGCTACTTCGACAGCAGCAGCGACGGCAAGAATGGCCAGACTGGCTATGCCTTCGACAACAACGGTGGCTACGCGAAGAACTCGGGCGAGGTCGACAACAAGACCTGGAGCGCCAt < 1:478395/136‑1 (MQ=255) aGTCGTTCAAGACTGACCTGCGCTACTTCGACAGCAGCAGCGACGGCAAGAATGGCCAGACTGGCTATGCCTTCGACAACAACGGTGGCTACGCGAAGAACTCGGGCGAGGTCGACAACAAGACCTGGAGCGCCAt < 1:459733/136‑1 (MQ=255) gTTCAAGACTGACCTGCGCTACTTCGACAGCAGCAGCGACGGCAAGAATGGCCAGACTGGCTATGCCTTCGACAACAACGGTGGCTACGCGAAGAACTCGGGCGAGGTCGACAACAAGACCTGGAGCGCCATGTTc < 1:253091/136‑1 (MQ=255) gACTGACCTGCGCTACTTCGACAGCAGCAGCGACGGCAAGAATGGCCAGACTGGCTATGCCTTCGACAACAACGGTGGCTACGCGAAGAACTCGGGCGAGGTCGACAACAAGACCTGGAGCGCCATGTTCACCTac < 1:132931/136‑1 (MQ=255) | GGTGATTACAAGCTCACCCCAGACCTGACTCTGCAGTACTACTACTCGAACCTGGAAGACTTCTACAAGCAGCACTTCCTGGGTGCGACCCACGTATTCAAGATTGCCGACAACCAGTCGTTCAAGACTGACCTGCGCTACTTCGACAGCAGCAGCGACGGCAAGAATGGCCAGACTGGCTATGCCTTCGACAACAACGGTGGCTACGCGAAGAACTCGGGCGAGGTCGACAACAAGACCTGGAGCGCCATGTTCACCTAC > NC_002947/289189‑289449 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |