| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 455,991 | G→T | 20.0% | R223R (CGG→AGG) | PP_0375 ← | prolyl oligopeptidase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 455,991 | 0 | G | T | 20.0% | 48.2 / 2.6 | 20 | R223R (CGG→AGG) | PP_0375 | prolyl oligopeptidase family protein |
| Reads supporting (aligned to +/- strand): ref base G (10/6); new base T (2/2); total (12/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
CAGGTACAGGTGCCCAATTGCCAAGGCGCAGCAGCATGGTCCGCCGGTGCAGCAGGCAACGGCTGCCATTGGCCATCGACCTCACCCCAGGGTTGCCAGAAGCCATTGCGGTCGGAAAGGCAGTAGAGCCGACCTTCGGCATCGAAGCGTGGCTGTTGCAGCGATTCTTCGGCGGCGGCTACACATTGCGCCGGTCCCCAGTGGCCGCTGGCATCGCGCACGCGGCACACCAACCGGGTGACGGTCCAGGGCTGCGCCGGGCGG > NC_002947/455861‑456124 | cAGGTACAGGTGCCCAATTGCCAAGGCGCAGCAGCATGGTCCGCCGGTGCAGCAGGCAACGGCTGCCATTGGCCAACGACCTCACCCCAGGGTTGACAGAAGCCATTGCGGTCGGAAAGGAAGTAGATCCGACCta > 2:497056/1‑135 (MQ=255) cccAATTGCCAAGGCGCAGCAGCATGGTCCGCCGGTGCAGCAGGCAACGGCTGCCATTGGCCATCGACCTCACCCCAGGGTTGCCAGAAGCCATTGCGGTCGGAAAGGCAGTAGAGCCGACCTTCGGCATCGAAGc > 1:525910/1‑136 (MQ=255) ccAATTGCCAAGGCGCAGCAGCATGGTCCGCCGGTGCAGCAGGCAACGGCTGCCATTGGCCATCGACCTCACCCCAGGGTTGCCAGAAGCCATTGCGGTCGGAAAGGCAGTAGAGCCGACCTTCGGCATCGAAGCg < 2:115979/136‑1 (MQ=255) aaTTGCCAAGGCGCAGCAGCATGGTCCGCCGGTGCAGCAGGCAACGGCTGCCATTGGCCATCGACCTCACCCCAGGGTTGCCAGAAGCCATTGCGGTCGGAAAGGCAGTAGAGCCTACCTTCGGCATCGAAGCGTg > 1:484729/1‑136 (MQ=255) aaTTGCCAAGGCGCAGCAGCATGGTCCGCCGGTGCAGCAGGCAACGGCTGCCATTGGCCATCGACCTCACCCCAGGGTTGCCAGAAGCCATTGCGGTCGGAAAGGCAGTAGAGCCGACCTTCGGCATCGAAGCGTg > 1:435356/1‑136 (MQ=255) tGGTCCGCCGGTGCAGCAGGCAACGGCTGCCATTGGCCATCGACCTCACCCCAGGGTTGCCAGAAGCCATTGCGGTCGGAAAGGCAGTAGAGCCGACCTTCGGCATCGAAGCGTGGCTGTTGCAGCGATTCTTcgg < 1:473458/136‑1 (MQ=255) tCCGCCGGTGCAGCAGGCAACGGCTGCCATTGGCCATCGACCTCACCCCAGGGTTGCCAGAAGCCATTGCGGTCGGAAAGGCAGTAGAGCCGACCTTCGGCATCGAAGCGTGGCTGTTGCAGCGATTCTTcggcgg > 2:459334/1‑136 (MQ=255) gccgGTGCAGCAGGCAACGGCTGCCATTGGCCATCGACCTCACCCCAGGGTTGCCAGAAGCCATTGCGGTCGGAAAGGCAGTAGAGCCGACCTTCGGCATCGAAGCGTGGCTGTTGCAGCGATTCTTcggcggcgg > 1:425387/1‑136 (MQ=255) aCCTCACCCCAGGGTTGCCAGAAGGCATTGCGGTCGGAAAGGCAGTAGAGCCGACCTTCGGCATCGAAGCGTGGCTGTTGCAGCGATTCTTCGGCGGCGGCTACACATTGCGCCGGTCCCCAGTGGCCGCTGGCAt > 2:367162/1‑136 (MQ=255) ccTCACCCCAGGGTTGCCAGAAGCCATTGCGGTCGGAAAGGCAGTAGAGCCTACCTTCGGCATCGAAGCGTGGCTGTTGCAGCGATTCTTCGGCGGCGGCTACACATTGCGCCGGTCCCCAGTGGCCGCTGGCATc > 2:124510/1‑136 (MQ=255) cccAGGGTTGCCAGAAGCCATTGCGGTCGGAAAGGCAGTAGAGCCGACCTTCGGCATCGAAGCGTGGCTGTTGCAGCGATTCTTCGGCGGCGGCTACACATTGCGCCGGTCCCCAGTGGCCGCTGGCATCGCGCAc > 1:271545/1‑136 (MQ=255) ccAGGGTTGCCAGAAGCCATTGCGGTCGGAAAGGCAGTAGAGCCGACCTTCGGCATCGAAGCGTGGCTGTTGCAGCGATTCTTCGGCGGCGGCTACACATTGCGCCGGTCCCCAGTGGCCGCTGGCATCGCGCAcg < 2:525910/136‑1 (MQ=255) cAGAAGCCATTGCGGTCGGAAAGGCAGTAGAGCCGACCTTCGGCATCGAAGCGTGGCTGTTGCAGCGATTCTTCGGCGGCGGCTACACATTGCGCCGGTCCCCAGTGGCCGCTGGCATCGCGCACGCGGCACACCa > 2:256513/1‑136 (MQ=255) gAAGCCATTGCGGTCGGAAAGGCAGTAGAGCCGACCTTCGGCATCGAAGCGTGGCTGTTGCAGCGATTCTTCGGCGGCGGCTACACATTGCGCCGGTCCCCAGTGGCCGCTGGCATCGCGCACGCGGCACACCAAc > 1:25112/1‑136 (MQ=255) ccATTGCGGTCGGAAAGGCAGTAGAGCCTACCTTCGGCATCGAAGCGTGGCTGTTGCAGCGATTCTTCGGCGGCGGCTACACATTGCGCCGGTCCCCAGTGGCCGCTGGCATCGCGCACGCGGCACACCAACCggg < 1:124510/136‑1 (MQ=255) ccATTGCGGTCGGAAAGGCAGTAGAGCCGACCTTCGGCATCGAAGCGTGGCTGTTGCAGCGATTCTTCGGCGGCGGCTACACATTGCGCCGGTCCCCAGTGGCCGCTGGCATCGCGCACGCGGCACACCAACCggg < 2:25112/136‑1 (MQ=255) gCGGTCGGAAAGGCAGTAGAGCCTACCTTCGGCATCGAAGCGTGGCTGTTGCAGCGATTCTTCGGCGGCGGCTACACATTGCGCCGGTCCCCAGTGGCCGCTGGCATCGCGCACGCGGCACACCAACCGGGTGACg < 2:484729/136‑1 (MQ=255) gCGGTCGGAAAGGCAGTAGAGCCGACCTTCGGCATCGAAGCGTGGCTGTTGCAGCGATTCTTCGGCGGCGGCTACACATTGCGCCGGTCCCCAGTGGCCGCTGGCATCGCGCACGCGGCACACCAACCGGGTGACg < 2:435356/136‑1 (MQ=255) gTAGAGCCGACCTTCGGCATCGAAGCGTGGCTGTTGCAGCGATTCTTCGGCGGCGTCTACACATTGCGCCGGTCCCCAGTGGCCGCTGGCATCGCGCACGCGGCACACCAACCGGGTGACGGTCCAGGGCTGCGcc < 1:497056/136‑1 (MQ=255) ccGACCTTCGGCATCGAAGCGTGGCTGTTGCAGCGATTCTTCGGCGGCGGCTACACATTGCGCCGGTCCCCAGTGGCCGCTGGCATCGCGCACGCGGCACACCAACCGGGTGACGGTCCAGGGCTGCGCCGGGCgg > 1:216801/1‑136 (MQ=255) | CAGGTACAGGTGCCCAATTGCCAAGGCGCAGCAGCATGGTCCGCCGGTGCAGCAGGCAACGGCTGCCATTGGCCATCGACCTCACCCCAGGGTTGCCAGAAGCCATTGCGGTCGGAAAGGCAGTAGAGCCGACCTTCGGCATCGAAGCGTGGCTGTTGCAGCGATTCTTCGGCGGCGGCTACACATTGCGCCGGTCCCCAGTGGCCGCTGGCATCGCGCACGCGGCACACCAACCGGGTGACGGTCCAGGGCTGCGCCGGGCGG > NC_002947/455861‑456124 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |