| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 498,893 | G→T | 30.7% | G122W (GGG→TGG) | uhpA → | two‑component system response regulator UhpA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 498,893 | 0 | G | T | 30.7% | 22.0 / 3.4 | 13 | G122W (GGG→TGG) | uhpA | two‑component system response regulator UhpA |
| Reads supporting (aligned to +/- strand): ref base G (4/5); new base T (2/2); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTGGCGGGCGTTATATTGACCCGTCGCTGGCCGACCGCATGGTGTTCGAAGTAGGCCTCACCGAATCCCGGCCGTTGCACACACTGCTCTCGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGC > NC_002947/498764‑499018 | cTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTGGCGGGCGtt > 2:348616/1‑136 (MQ=255) gATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTGGCGGGCGTTATATTGACCAGTCGCTgg < 1:128389/136‑1 (MQ=255) gCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTGGCGGGCGTTATATTGACCCGTCGCTGGCCGAcc < 2:101175/136‑1 (MQ=255) gcgcGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTGGCGGGCGTTATATTGACCCGTCGCTGGCCGACCGCATGGTGTTCg > 1:95182/1‑136 (MQ=255) gcgcTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTGGCGGGCGTTATATTGACCCGTCGCTGGCCGACCGCATGGTGTTCGAAGTAg < 1:348616/136‑1 (MQ=255) cgcTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTggcgg < 1:129093/88‑1 (MQ=255) cgcTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTggcgg > 2:129093/1‑88 (MQ=255) cggGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTGGCTGGCGTTATATTGACCCGTCGCTGGCCGACCGCATGGTGTTCGAAGTAGGCCTCACCGAATCCCGGcc > 1:193139/1‑136 (MQ=255) cggGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTGGCTGGCGTTATATTGACCCGTCGCTGGCCGACCGCATGGTGTTCGAAGTAGGCCTCACCGAATCCCGGcc > 1:450557/1‑136 (MQ=255) tGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTGGCGGGCGTTATATTGACCCGTCGCTGGCCGACCGCATGGTGTTCGAAGTAGGCCTCACCGAATCCCGGCCGTTGcac < 1:263370/136‑1 (MQ=255) aGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTGGCGGGCGTTATATTGa > 1:106098/1‑66 (MQ=255) aGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTGGCGGGCGTTATATTGa < 2:106098/66‑1 (MQ=255) gATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTGGCGGGCGTTATATTGACCCGTCGCTGGCCGACCGCATGGTGTTCGAAGTAGGCCTCACCGAATCCCGGCCGTTGCACACACTGCTCTCGGAGc > 2:188780/1‑136 (MQ=255) ggCCGGTGGCTGGCGTTATATTGACCCGTCGCTGGCCGACCGCATGGTGTTCGAAGTAGGCCTCACCGAATCCCGGCCGTTGCACACACTGCTCTCGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGgc < 2:193139/136‑1 (MQ=255) ggCCGGTGGCTGGCGTTATATTGACCCGTCGCTGGCCGACCGCATGGTGTTCGAAGTAGGCCTCACCGAATCCCGGCCGTTGCACACACTGCTCTCGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGgc < 2:450557/136‑1 (MQ=255) | CTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTGGCGGGCGTTATATTGACCCGTCGCTGGCCGACCGCATGGTGTTCGAAGTAGGCCTCACCGAATCCCGGCCGTTGCACACACTGCTCTCGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGC > NC_002947/498764‑499018 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |