Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 501,457 C→T 13.8% Q280* (CAG→TAG)  PP_0412 → polyamine ABC transportersubstrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947501,4570CT13.8% 75.8 / 3.7 29Q280* (CAG→TAG) PP_0412polyamine ABC transportersubstrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (11/14);  new base T (2/2);  total (13/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01

GAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTCGAAGAGGCGAAGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACGCCTGAGAACATTGCCAACCAGGTGCAGATCGAC  >  NC_002947/501333‑501585
                                                                                                                            |                                                                                                                                
gagCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTCGAAGAGGCGAAGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTAc                                                                                                                       >  2:446591/1‑136 (MQ=255)
   cAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTCGAAGAGGCGAAGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGTAGAAGATCTACTcc                                                                                                                    >  2:461144/1‑136 (MQ=255)
   cAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTCGAAGAGGCGAAGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGTAGAAGATCTACTcc                                                                                                                    >  2:458528/1‑136 (MQ=255)
   cAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTCGAAGAGGCGAAGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTcc                                                                                                                    >  1:392394/1‑136 (MQ=255)
        tCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTCGAAGAGGCGAAGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGaaaa                                                                                                               >  1:350813/1‑136 (MQ=255)
        tCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTCGAAGAGGCGAAGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGaaaa                                                                                                               <  1:446591/136‑1 (MQ=255)
        tCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTCGAAGAGGCGAAGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGaaaa                                                                                                               >  1:512307/1‑136 (MQ=255)
         cAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTCGAAGAGGCGAAGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAAc                                                                                                              <  2:46385/136‑1 (MQ=255)
                   tgGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTCGAAGAGGCGAAGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATg                                                                                                    >  1:215206/1‑136 (MQ=255)
                    ggAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTCGAAGAGGCGAAGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATgg                                                                                                   >  2:465952/1‑136 (MQ=255)
                        cggcggCATCTTCGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTCGAAGAGGCGAAGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCg                                                                                               <  1:324059/136‑1 (MQ=255)
                        cggcggCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTCGAAGAGGCGAAGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCg                                                                                               <  1:447755/136‑1 (MQ=255)
                              cATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTCGAAGAGGGGAAGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAAc                                                                                         <  2:114720/136‑1 (MQ=255)
                              cATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTCGAAGAGGCGAAGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCg                                                                                               >  1:251420/1‑130 (MQ=255)
                              cATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTCGAAGAGGCGAAGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCg                                                                                               <  2:251420/130‑1 (MQ=255)
                                           gATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTCGAAGAGGCGAAGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCACGGCCAACTCCAAGGCCGTGa                                                                            <  1:465952/136‑1 (MQ=255)
                                             tGCATGGGCAATTCCCAAAGGCGCCAAAAACGTCGAAGAGGCGAAGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGc                                                                          >  2:418501/1‑136 (MQ=255)
                                                          cccAAAGGCGCCAAAAACGTCGAAGAGGCGAAGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACg                                                             <  1:264518/136‑1 (MQ=255)
                                                                        aaaCGTCGAAGAGGCGAAGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGACTGCTGTCGGACGAAGTGAAGAAAGAc                                               >  2:441033/1‑136 (MQ=255)
                                                                                    ggCGAAGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACg                                   <  1:395612/136‑1 (MQ=255)
                                                                                      cGAAGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACGcc                                 <  2:350813/136‑1 (MQ=255)
                                                                                      cGAAGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACGcc                                 <  2:512307/136‑1 (MQ=255)
                                                                                               tCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACGCCTGAGAACAt                        <  1:353745/136‑1 (MQ=255)
                                                                                                 aTCGCCTACAGCGTCAAGCCCGAGCAGTAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACGCCTGAGAACATTg                      <  1:461144/136‑1 (MQ=255)
                                                                                                 aTCGCCTACAGCGTCAAGCCCGAGCAGTAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACGCCTGAGAACATTg                      <  1:458528/136‑1 (MQ=255)
                                                                                                         cAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACGCCTGAGAACATTGCCAACCAg              <  1:418501/136‑1 (MQ=255)
                                                                                                          aGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACGCCTGAGAACATTGCCAACCAgg             >  1:85486/1‑136 (MQ=255)
                                                                                                                 aGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACGCCTGAGAACATTGCCAACCAGGTGCAGAt      >  1:266739/1‑136 (MQ=255)
                                                                                                                     cGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACGCCTGAGAACATTGCCAACCAGGTGCAGATCGAc  <  2:392394/136‑1 (MQ=255)
                                                                                                                          agcagAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACGCCTGAGAACATTGCCAACCAGGTGCAGATCGAc  <  2:26963/131‑1 (MQ=255)
                                                                                                                          agcagAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACGCCTGAGAACATTGCCAACCAGGTGCAGATCGAc  >  1:26963/1‑131 (MQ=255)
                                                                                                                            |                                                                                                                                
GAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTCGAAGAGGCGAAGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACGCCTGAGAACATTGCCAACCAGGTGCAGATCGAC  >  NC_002947/501333‑501585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑