| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 539,661 | A→G | 100% | D679G (GAC→GGC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 539,661 | 0 | A | G | 100.0% | 66.8 / NA | 19 | D679G (GAC→GGC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (6/13); total (6/13) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCTGGTAGCAGTTCGTCACCTGAACGAATTCACCGTCAAGGCGCCGGAAGACGTCACCCTGATGGACGTTTCGCCGAAGCAGGTTGTTTCCGTTGCAGCGTCGCTGATTCCGTTCCTCGAGCACGACGACGCCAACCGTGCGTTGATGGGTTCGAACATGCAGCGTCAGGCTGTACCGACCCTGCGTGCCGACAAGCCGCTGGTAGGTACCGGCATGGAGCGCAACGTTGCCCGTGACTCCGGTGTCTGCGTGGT > NC_002947/539536‑539790 | gCTGGTAGCAGTTCGTCACCTGAACGAATTCACCGTCAAGGCGCCGGAAGACGTCACCCTGATGGACGTTTCGCCGAAGCAGGTTGTTTCCGTTGCAGCGTCGCTGATTCCGTTCCTCGAGCACGGCGACGCCAAc < 2:422439/136‑1 (MQ=255)gCTGGTAGCAGTTCGTCACCTGAACGAATTCACCGTCAAGGCGCCGGAAGACGTCACCCTGATGGACGTTTCGCCGAAGCAGGTTGTTTCCGTTGCAGCGTCGCTGATTCCGTTCCTCGAGCACGGCGACGCCAAc < 2:333918/136‑1 (MQ=255) gTAGCAGTTCGTCACCTGAACGAATTCACCGTCAAGGCGCCGGAAGACGTCACCCTGATGGACGTTTCGCCGAAGCAGGTTGTTTCCGTTGCAGCGTCGCTGATTCCGTTCCTCGAGCACGGCGACGCCAACCGTg < 2:535355/136‑1 (MQ=255) cGTCACCTGAACGAATTCACCGTCAAGGCGCCGGAAGACGTCAGCCTGATGGACGTTTCGCCGAAGCAGGTTGTTTCCGTTGCAGCGTCGCTGATTCCGTTCCTCGAGCACGGCGACGCCAACCGTGCGTTGATgg < 1:363295/136‑1 (MQ=255) cGTCACCTGAACGAATTCACCGTCAAGGCGCCGGAAGACGTCACCCTGATGGACGTTTCGCCGAAGCAGGTTGTTTCCGTTGCAGCGTCGCTGATTCCGTTCCTCGAGCACGGCGACGCCAACCGTGCGTTGATgg > 1:179357/1‑136 (MQ=255) cGTCACCTGAACGAATTCACCGTCAAGGCGCCGGAAGACGTCACCCTGATGGACGTTTCGCCGAAGCAGGTTGTTTCCGTTGCAGCGTCGCTGATTCCGTTCCTCGAGCACGGCGACGCCAACCGTGCGTTGATgg < 2:406175/136‑1 (MQ=255) gTCACCTGAACGAATTCACCGTCAAGGCGCCGGAAGACGTCACCCTGATGGACGTTTCGCCGAAGCAGGTTGTTTCCGTTGCAGCGTCGCTGATTCCGTTCCTCGAGCACGGCGACGCCAACCGTGCGTTGATggg > 1:321701/1‑136 (MQ=255) aaCGAATTAACCGTCAAGGCGCCGGAAGACGTCACCCTGATGGACGTTTCGCCGAAGCAGGTTGTTTCCGTTGCAGCGTCGCTGATTCCGTTCCTCGAGCACGGCGACGCCAACCGTGCGTTGATGGGTTCGAACa < 1:515833/136‑1 (MQ=255) aaTTCACCGTCAAGGCGCCGGAAGACGTCACCCTGATGGACGTTTCGCCGAAGCAGGTTGTTTCCGTTGCAGCGTCGCTGATTCCGTTCCTCGAGCACGGCGACGCCAACCGTGCGTTGATGGGTTCGAACATGCa < 1:426503/136‑1 (MQ=255) aaTTCACCGTCAAGGCGCCGGAAGACGTCACCCTGATGGACGTTTCGCCGAAGCAGGTTGTTTCCGTTGCAGCGTCGCTGATTCCGTTCCTCGAGCACGGCGACGCCAACCGTGCGTTGATGGGTTCGAACATGCa < 1:230522/136‑1 (MQ=255) aaTTCACCGTCAAGGCGCCGGAAGACGTCACCCTGATGGACGTTTCGCCGAAGCAGGTTGTTTCCGTTGCAGCGTCGCTGATTCCGTTCCTCGAGCACGGCGACGCCAACCGTGCGTTGATGGGTTCGAACATGCa < 1:166681/136‑1 (MQ=255) aTTCACCGTCAAGGCGCCGGTAGACGTCACCCTGATGGACGTTTCGCCGAAGCAGGTTGTTTCCGTTGCAGCGTCGCTGATTCCGTTCCTCGAGCACGGCGACGCCAACCGTGCGTTGATGGGTTCGAACATGCAg < 1:52201/136‑1 (MQ=255) gAAGACGTCACCCTGATGGACGTTTCGCCGAAGCAGGTTGTTTCCGTTGCAGCGTCGCTGATTCCGTTCCTCGAGCACGGCGACGCCAACCGTGCGTTGATGGGTTCGAACATGCAGCGTCAGGCTGTACCGAccc > 1:81926/1‑136 (MQ=255) aCGTTTCGCCGAAGCAGGTTGTTTCCGTTGCAGCGTCGCTGATTCCGTTCCTCGAGCACGGCGACGCCAACCGTGCGTTGATGGGTTCGAACATGCAGCGTCAGGCTGTACCGACCCTGCGTGCCGACAAGCCGCt < 1:244963/136‑1 (MQ=255) tCGCCGAAGCAGGTTGTTTCCGTTGCAGCGTCGCTGATTCCGTTCCTCGAGCACGGCGACGCCAACCGTGCGTTGATGGGTTCGAACATGCAGCGTCAGGCTGTACCGACCCTGCGTGCCGACAAGCCGCTggtag < 1:322544/136‑1 (MQ=255) gCCGAAGCAGGTTGTTTCCGTTGCAGCGTCGCTGATTCCGTTCCTCGAGCACGGCGACGCCAACCGTGCGTTGATGGGTTCGAACATGCAGCGTCAGGCTGTACCGACCCTGCGTGCCGACa < 1:475939/122‑1 (MQ=255) gCCGAAGCAGGTTGTTTCCGTTGCAGCGTCGCTGATTCCGTTCCTCGAGCACGGCGACGCCAACCGTGCGTTGATGGGTTCGAACATGCAGCGTCAGGCTGTACCGACCCTGCGTGCCGACa > 2:475939/1‑122 (MQ=255) tgttTCCGTTGCAGCGTCGCTGATTCCGTTCCTCGAGCACGGCGACGCCAACCGTGCGTTGATGGGTTCGAACATGCAGCGTCAGGCTGTACCGACCCTGCGTGCCGACAAGCCGCTGGTAGGTACCGGCATGGAg > 2:1129/1‑136 (MQ=255) aGCACGGCGACGCCAACCGTGCGTTGATGGGTTCGAACATGCAGCGTCAGGCTGTACCGACCCTGCGTGCCGACAAGCCGCTGGTAGGTACCGGCATGGAGCGCAACGTTGCCCGTGACTCCGGTGTCTGCGTGGt > 2:400853/1‑136 (MQ=255) | GCTGGTAGCAGTTCGTCACCTGAACGAATTCACCGTCAAGGCGCCGGAAGACGTCACCCTGATGGACGTTTCGCCGAAGCAGGTTGTTTCCGTTGCAGCGTCGCTGATTCCGTTCCTCGAGCACGACGACGCCAACCGTGCGTTGATGGGTTCGAACATGCAGCGTCAGGCTGTACCGACCCTGCGTGCCGACAAGCCGCTGGTAGGTACCGGCATGGAGCGCAACGTTGCCCGTGACTCCGGTGTCTGCGTGGT > NC_002947/539536‑539790 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |