| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,080,607 | A→G | 14.8% | H124R (CAC→CGC) | mreB → | cell wall structural actin‑like protein MreB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,080,607 | 0 | A | G | 14.8% | 61.9 / 5.2 | 27 | H124R (CAC→CGC) | mreB | cell wall structural actin‑like protein MreB |
| Reads supporting (aligned to +/- strand): ref base A (9/14); new base G (2/2); total (11/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01 | |||||||||||
CCGTCGGTACCGAAGCCAAACGCATGCTGGGCCGTACGCCCGGCAACATTGCAGCCATTCGTCCGATGAAGGACGGCGTCATTGCCGACTTCAGCGTCTGCGAAAAAATGTTGCAATATTTTATCAACAAGGTTCACGAGAACAGCTTCCTGCAGCCGAGCCCGCGCGTGCTGATCTGCGTGCCGTGCAAGTCGACCCAGGTCGAGCGCCGCGCCATCCGCGAGTCGGCCCTCGGCGCGGGTGCCCGTGAAGTGTTCCTGATCGAAGAGCC > NC_002947/1080472‑1080742 | ccGTCGGTACCGAAGCCAAACGCATGCTGGGCCGTACGCCCGGCAACATTGCAGCCATTCGTCCGATGAAGGACGGCGTCATTGCCGACTTCAGCGTCTGCGAAAAAATGTTGCAATATTTTATCAACAAGGTTCa > 2:38438/1‑136 (MQ=255) ggTACCGAAGCCAAACGCATGCTGGGCCGTACGCCCGGCAACATTGCAGCCATTCGTCCGATGAAGGACGGCGTCATTGCCGACTTCAGCGTCTGCGAAAAAATGTTGCAATATTTTATCAACAAGGTTCACgaga > 1:230705/1‑136 (MQ=255) gTACCGAAGCCAAACGCATGCTGGGCCGTACGCCCGGCAACATTGCAGCCATTCGTCCGATGAAGGACGGCGTCATTGCCGACTTCAGCGTCTGCGAAAAAATGTTGCAATATTTTATCAACAAGGTTCACGAGaa < 1:227650/136‑1 (MQ=255) cTGGGCCGTACGCCCGGCAACATTGCAGCCATTCGTCCGATGAAGGACGGCGTCATTGCCGACTTCAGCGTCTGCGAAAAAATGTTGCAATATTTTATCAACAAGGTTCACGAGAACAGCTTCCTGCAGCCGAGcc < 1:226213/136‑1 (MQ=255) tACGCCCGGCAACATTGCAGCCATTCGTCCGATGAAGGACGGCGTCATTGCCGACTTCAGCGTCTGCGAAAAAATGTTGCAATATTTTATCAACAAGGTTCACGAGAACAGCTTCCTGCAGCCGAGCCCGCGCGTg > 1:372468/1‑136 (MQ=255) gCCCGGCAACATTGCAGCCATTCGTCCGATGAAGGACGGCGTCATTGCCGACTTCAGCGTCTGCGAAAAAATGTTGCAATATTTTATCAACAAGGTTCACGAGAACAGCTTCCTGCAGCCGAGCCCGCGCGTGCTg > 1:60526/1‑136 (MQ=255) ccGGCAACATTGCAGCCATTCGTCCGATGAAGGACGGCGTCATTGCCGACTTCAGCGTCTGCGAAAAAATGTTGCAATATTTTATCAACAAGGTTCACGAGAACAGCTTCCTGCAGCCGAGCCCGCGCATGCTGAt > 2:385504/1‑136 (MQ=255) gCAACATTGCAGCCATTCGTCCGATGAAGGACGGCGTCATTGCCGACTTCAGCGTCTGCGAAAAAATGTTGCAATATTTTATCAACAAGGTTCGCGAGAACAGCTTCCTGCAGCCGAGCCCGCGCGTGCTGATCTg > 1:255343/1‑136 (MQ=255) gCAACATTGCAGCCATTCGTCCGATGAAGGACGGCGTCATTGCCGACTTCAGCGTCTGCGAAAAAATGTTGCAATATTTTATCAACAAGGTTCGCGAGAACAGCTTCCTGCAGCCGAGCCCGCGCGTGCTGATCTg > 1:257780/1‑136 (MQ=255) gCAACATTGCAGCCATTCGTCCGATGAAGGACGGCGTCATTGCCGACTTCAGCGTCTGCGAAAAAATGTTGCAATATTTTATCAACAAGGTTCACGAGAACAGCTTCCTGCAGCCGAGCCCGCGCGTGCTGATCTg < 2:444612/136‑1 (MQ=255) cAACATTGCAGCCATTCGTCCGATGAAGGACGGCGTCATTGCCGACTTCAGCGTCTGCGAAAAAATGTTGCAATATTTTATCAACAAGGTTCACGAGAACAGCTTCCTGCAGCCGAGCCCGCGCGTGCTGATCTGc > 2:216347/1‑136 (MQ=255) cAACATTGCAGCCATTCGTCCGATGAAGGACGGCGTCATTGCCGACTTCAGCGTCTGCGAAAAAATGTTGCAATATTTTATCAACAAGGTTCACGAGAACAGCTTCCTGCAGCCGAGCCCGCGCGTGCTGATCTGc < 1:442256/136‑1 (MQ=255) aaCATTGCAGCCATTCGTCCGATGAAGGACGGCGTCATTGCCGACTTCAGCGTCTGCGAAAAAATGTTGCAATATTTTATCAACAAGGTTCACGAGAACAGCTTCCTGCAGCCGAGCCCGCGCGTGCTGATCTGcg > 1:502939/1‑136 (MQ=255) cGTCCGATGAAGGACGGCGTCATTGCCGACTTCAGCGTCTGCGAAAAAATGTTGCAATATTTTATCAACAAGGTTCACGAGAACAGCTTCCTGCAGCCGAGCCCGCGCGTGCTGATCTGCGTGCCGTGCAAGTCGa < 2:465699/136‑1 (MQ=255) gTCCGATGAAGGACGGCGTCATTGCCGACTTCAGCGTCTGCGAAAAAATGTTGCAATATTTTATCAACAAGGTTCACGAGAACAGCTTCCTGCAGCCGAGCCCGCGCGTGCTGATCTGCGTGCCGTGCAAGTCGAc > 1:247606/1‑136 (MQ=255) gATGAAGGACGGCGTCATTGCCGACTTCAGCGTCTGCGAAAAAATGTTGCAATATTTTATCAACAAGGTTCGCGAGAACAGCTTCCTGCAGCCGAGCCCGCGCGTGCTGATCTGCGTGCCGTGCAAGTCGACCCAg < 2:257780/136‑1 (MQ=255) gATGAAGGACGGCGTCATTGCCGACTTCAGCGTCTGCGAAAAAATGTTGCAATATTTTATCAACAAGGTTCGCGAGAACAGCTTCCTGCAGCCGAGCCCGCGCGTGCTGATCTGCGTGCCGTGCAAGTCGACCCAg < 2:255343/136‑1 (MQ=255) gATGAAGGACGGCGTCATTGCCGACTTCAGCGTCTGCGAAAAAATGTTGCAATATTTTATCAACAAGGTTCACGAGAACAGCTTCCTGCAGCCGAGCCCGCGCGTGCTGATCTGCGTGCCGTGCAAGTCGACCCAg < 2:248072/136‑1 (MQ=255) tGAAGGACGGCGTCATTGCCGACTTCAGCGTCTGCGAAAAAATGTTGCAATATTTTATCAACAAGGTTCACGAGAACAGCTTCCTGCAGCCGAGCCCGCGCGTGCTGATCTGCGTGCCGTGCAAGTCGACCCAGGt < 1:216347/136‑1 (MQ=255) gACTTCAGCGTCTGCGAAAAAATGTTGCAATATTTTATCAACAAGGTTCACGAGAACAGCTTCCTGCAGCCGAGCCCGCGCGTGCTGATCTGCGTGCCGTGCAAGTCGACCCAGGTCGAGCGCCGCGCCATCcgcg < 2:247606/136‑1 (MQ=255) cAGCGTCTGCGAAAAAATGTTGCAATATTTTATCAACAAGGTTCACGAGAACAGCTTCCTGCAGCCGAGCCCGCGCGTGCTGATCTGCGTGCCGTGCAAGTCGACCCAGGTCGAGCGCCGCGCCATCCGCGAGTCg < 1:118899/136‑1 (MQ=255) cAGCGTCTGCGAAAAAATGTTGCAATATTTTATCAACAAGGTTCACGAGAACAGCTTCCTGCAGCCGAGCCCGCGCATGCTGATCTGCGTGCCGTGCAAGTCGACCCAGGTCGAGCGCCGCGCCATCCGCGAGTCg < 1:385504/136‑1 (MQ=255) aaaaaaTGTTGCAATATTTTATCAACTAGGTTCACGAGAACAGCTTCCTGCAGCCGAGCCCGCGCGTGCTGATCTGCGTGCCGTGCAAGTCGACCCAGGTCGAGCGCCGCGCCATCCGCGAGTCGGCCCTCGgcgc < 1:234044/136‑1 (MQ=255) aaaaaaTGTTGCAATATTTTATCAACAAGGTTCACGAGAACAGCTTCCTGCAGCCGAGCCCGCGCGTGCTGATCTGCGTGCCGTGCAAGTCGACCCAGGTCGAGCGCCGCGCCATCCGCGAGTCGGCCCTCGgcgc > 2:281791/1‑136 (MQ=255) aaaaaaTGTTGCAATATTTTATCAACAAGGTTCACGAGAACAGCTTCCTGCAGCCGAGCCCGCGCGTGCTGATCTGCGTGCCGTGCAAGTCGACCCAGGTCGAGCGCCGCGCCATCCGCGAGTCGGCCCTCGgcgc < 2:101017/136‑1 (MQ=255) aaaaaaTGTTGCAATATTTTATCAACAAGGTTCACGAGAACAGCTTCCTGCAGCCGAGCCCGCGCGTGCTGATCTGCGTGCCGTGCAAGTAGACCCAGGTCGAGCGCCGCGCCATCCGCGAGTCGGCCCTCGgcgc > 2:192832/1‑136 (MQ=255) tGTTGCAATATTTTATCAACAAGGTTCACGAGAACAGCTTCCTGCAGCCGAGCCCGCGCGTGCTGATCTGCGTGCCGTGCAAGTCGACCCAGGTCGAGCGCCGCGCCATCCGCGAGTCGGCCCTCGGCGCGGGTGc < 1:103443/136‑1 (MQ=255) cAATATTTTATCAACAAGGTTCACGAGAACAGCTTCCTGCAGCCGAGCCCGCGCGTGCTGATCTGCGTGCCGTGCAAGTCGACCCAGGTCGAGCGCCGCGCCATCCGCGAGTCGGCCCTCGGCGCGGGTGCCCGTg < 2:60526/136‑1 (MQ=255) aCGAGAAGAGCTTCCTGCAGCCGAGCCCGCGCGTGCTGATCTGCGTGCCGTGCAAGTCGACCCAGGTCGAGCGCCGCGCCATCCGCGAGTCGGCCCTCGGCGCGGGTGCCCGTGAAGTGTTCCTGATCGAAGAGcc < 2:316088/136‑1 (MQ=255) | CCGTCGGTACCGAAGCCAAACGCATGCTGGGCCGTACGCCCGGCAACATTGCAGCCATTCGTCCGATGAAGGACGGCGTCATTGCCGACTTCAGCGTCTGCGAAAAAATGTTGCAATATTTTATCAACAAGGTTCACGAGAACAGCTTCCTGCAGCCGAGCCCGCGCGTGCTGATCTGCGTGCCGTGCAAGTCGACCCAGGTCGAGCGCCGCGCCATCCGCGAGTCGGCCCTCGGCGCGGGTGCCCGTGAAGTGTTCCTGATCGAAGAGCC > NC_002947/1080472‑1080742 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |