Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,241,784	T→C	12.1%	F113F (TTT→TTC)	ybaK →	Cys‑tRNAPro deacylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,241,784	0	T	C	12.1%	81.4 / 4.7	33	F113F (TTT→TTC)	ybaK	Cys‑tRNAPro deacylase
Reads supporting (aligned to +/- strand):  ref base T (17/12);  new base C (2/2);  total (19/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.27e-01

CCTGGCCCATGCTGCCGGGGTAAAAAAGTGCGAGATGGCCGACCCGGCAGCGGCGCAGCGGGCGACCGGTTACCTGGTGGGCGGCATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACCTTTATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGG  >  NC_002947/1241661‑1241912                                                                                                                            |                                                                                                                                 ccTGGCCCATGCTGCCGGGGTAAAAAAGTGCGAGATGGCCGACCCGGCAGCGGCGCAGCGGGCGACCGGTTACCTGGTGGGCGGCATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACCTTTATTGATGACTCg                                                                                                                      <  2:359459/136‑1 (MQ=255)      cccATGCTGCCGGGGTAAAAAAGTGCGAGATGGCCGACCCGGCAGCGGCGCAGCGGGCGACCGGTTACCTGGTGGGCGGCATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACCTTTATTGATGACTCGGCGCa                                                                                                                 >  2:551341/1‑136 (MQ=255)      cccATGCTGCCGGGGTAAAAAAGTGCGAGATGGCCGACCCGGCAGCGGCGCAGCGGGCGACCGGATACCTGGTGGGCGGCATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACCTTTATTGATGACTCGGCGCa                                                                                                                 >  1:372199/1‑136 (MQ=255)            ctgcCGGAGTAAAAAAGTGCGAGATGGCCGACCCGGCAGCGGCGCAGCGGGCGACCGGTTACCTGGTGGGCGGCATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACCTTTATTGATGACTCGGCGCAGAAttt                                                                                                           >  2:504317/1‑136 (MQ=255)             tgcCGGGGTAAAAAAGTGCGAGATGGCCGACCCGGCAGCGGCGCAGCGGGCGACCGGTTACCTGGTGGGCGGCATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACCTTTATTGATGACTCGGCGCAGAAtttt                                                                                                          >  2:534251/1‑136 (MQ=255)             tgcCGGGGTAAAAAAGTGCGAGATGGCCGACCCGGCAGCGGCGCAGCGGGCGACCGGTTACCTGGTGGGCGGCATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACCTTTATTGATGACTCGGCGCAGAAtttt                                                                                                          >  2:457375/1‑136 (MQ=255)             tgcCGGGGTAAAAAAGTGCGAGATGGCCGACCCGGCAGCGGCGCAGCGGGCGACCGGTTACCTGGTGGGCGGCATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACCTTTATTGATGACTCGGCGCAGAAtttt                                                                                                          >  1:56898/1‑136 (MQ=255)             tgcCGGGGTAAAAAAGTGCGAGATGGCCGACCCGGCAGCGGCGCAGCGGGCGACCGGTTACCTGGTGGGCGGCATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACCTTTATTGATGACTCGGCGCAGAAtttt                                                                                                          >  1:90239/1‑136 (MQ=255)                  gggTAAAAAAGTGCGAGATGGCCGACCCGGCAGCGGCGCAGCGGGCGACCGGTTACCTGGTGGGCGGCATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACCTTTATTGATGACTCGGCGCAGAATTTTGCAAc                                                                                                     >  1:366336/1‑136 (MQ=255)                    gTAAAAAAGTGCGAGATGGCCGACCCGGCAGCGGCGCAGCGGGCGACCGGTTACCTGGTGGGCGGCATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACCTTTATTGATGACTCGGCGCAGAATTTTGCAACTa                                                                                                   >  2:324495/1‑136 (MQ=255)                                 agaTGGCCGACCCGGCAGCGGCGCAGCGGGCGACCGGTTACCTGGTGGGCGGCATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACC‑‑CATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCg                                                                                    >  1:297388/1‑136 (MQ=255)                                 agaTGGCCGACCCGGCAGCGGCGCAGCGGGCGACCGGTTACCTGGTGGGCGGCATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACC‑‑CATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCg                                                                                    >  1:521569/1‑136 (MQ=255)                                         gACCCGGCAGCGGCGCAGCGGGCGACCGGTTACCTGGTGGGCGGCATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACCTTTATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGc                                                                              >  1:173454/1‑136 (MQ=255)                                         gACCCGGCAGCGGCGCAGCGGGCGACCGGTTACCTGGTGGGCGGCATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACCTTTATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGc                                                                              >  1:6464/1‑136 (MQ=255)                                                 aGCGGCGCAGCGGGCGACCGGTTACCTGGTGGGCGGCATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACCTTTATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTg                                                                      >  2:373645/1‑136 (MQ=255)                                                            gggCGACCGGTTACCTGGTGGGCGGCATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACCTTTATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGtt                                                           <  2:56898/136‑1 (MQ=255)                                                            gggCGACCGGTTACCTGGTGGGCGGCATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACCTTTATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGtt                                                           >  1:68288/1‑136 (MQ=255)                                                                 aCCGGTTACCTGGTGGGCGGCATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACCTTTATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCg                                                      <  2:366336/136‑1 (MQ=255)                                                                   cGGTTACCTGGTGGGCGGCATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACCTTTATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGtt                                                           >  2:372133/1‑129 (MQ=255)                                                                   cGGTTACCTGGTGGGCGGCATCAGCCCGCGGGGGCAGAAGAAGCGCCTGCGGACCTTTATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGtt                                                           <  1:372133/129‑1 (MQ=255)                                                                          cTGGTGGGCGGCATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACCTTTATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCATCGCCGGCAGTCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGc                                             <  2:372199/136‑1 (MQ=255)                                                                           tggtggGCGGCATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACCTTTATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCt                                            <  2:492123/136‑1 (MQ=255)                                                                                 gcggcATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACC‑‑CATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGt                                    <  2:297388/136‑1 (MQ=255)                                                                                 gcggcATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACC‑‑CATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGt                                    <  2:521569/136‑1 (MQ=255)                                                                                     cATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACCTTTATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGtat                                  <  1:534251/136‑1 (MQ=255)                                                                                               cTGGGGCAGAAGAAGCGCCTGCGGACCTTTATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCa                        <  1:457375/136‑1 (MQ=255)                                                                                                           aagCGCCTGCGGACCTTTATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGa            >  2:54516/1‑136 (MQ=255)                                                                                                             gcgcCTGCGGACCTTTATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGAtt          <  1:324495/136‑1 (MQ=255)                                                                                                               gcCTGCGGACCTTTATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTgg        <  1:180811/136‑1 (MQ=255)                                                                                                                ccTGCGGACCTTTATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGt       >  1:321964/1‑136 (MQ=255)                                                                                                                    gggACCTTTATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAg   <  2:502610/135‑1 (MQ=255)                                                                                                                    cGGACCTTTATTGATGACTCGGCGAAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAg   <  1:212133/136‑1 (MQ=255)                                                                                                                     ggACCTTTATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAgg  >  1:311595/1‑136 (MQ=255)                                                                                                                            |                                                                                                                                 CCTGGCCCATGCTGCCGGGGTAAAAAAGTGCGAGATGGCCGACCCGGCAGCGGCGCAGCGGGCGACCGGTTACCTGGTGGGCGGCATCAGCCCGCTGGGGCAGAAGAAGCGCCTGCGGACCTTTATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGG  >  NC_002947/1241661‑1241912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑