| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,279,499 | G→T | 21.1% | intergenic (‑459/+23) | PP_1115 ← / ← PP_5462 | lipoprotein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,279,499 | 0 | G | T | 21.1% | 40.0 / 2.6 | 19 | intergenic (‑459/+23) | PP_1115/PP_5462 | lipoprotein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (8/7); new base T (2/2); total (10/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.53e-01 | |||||||||||
CACCTCGGAAGCGTCAGTGGCCATCCAGATGAATAGGGCAGCAGTTGCAATTGCGATAGCTACGTAATACAACTCGTTCTTATCTATTGATGAGTTCATGTTCAGCCTCCATTCAGATTGAGCTACTGCAAAGTGGCTTTAGCGGTCCCCTTTGCTGTCATTCCGTGCGCGATATTGGGAATAGCTCCTCTGGATTGCGTGATTCAGAGTTCATTTTTAAGCACTCCGCTTCACCTGCCGACCTTGTTGGGAATCCACGCTTGAGTCG > NC_002947/1279365‑1279632 | cACCTCGGAAGCGTCAGTGGCCATCCAGATGAATAGGGCAGCAGTTGCAATTGCGATAGCTACGTAATACAACTCGTTCTTATCTATTGATGAGTTCATGTTCAGCCTCCATTCAGATTGAGCTACTGCAAAGTgg < 2:36581/136‑1 (MQ=255)cACCTCGCAACCGTCAGTGGCCATCCAGAGCAATAGCGCAGCAGTTGCAATTGCGATAGCTACGTAATACAACTCGTTCTTATCTATTGATGAGTTCATGTTCAGCCTCCATTCAGATTGAGCTACTGCAAAGTgg < 2:212959/136‑1 (MQ=255) cGGAAGCGTCAGTGGCCATCCAGATGAATAGGGCAGCAGTTGCAATTGCGATAGCTACGTAATACAACTCGTTCTTATCTATTGATGAGTTCATGTTCAGCCTCCATTCAGATTGAGCTACTGCAAAGTGGCTTTa > 1:106774/1‑136 (MQ=255) gAAGCGTCAGTGGCCATCCAGATGAATAGGGCAGCAGTTGCAATTGCGATAGCTACGTAATACAACTCGTTCTTATCTATTGATGAGTTCATGTTCAGCCTCCATTCAGATTGAGCTACTGCAAAGTGGCTTTAGc < 2:106774/136‑1 (MQ=255) aTAGGGCAGCAGTTGCAATTGCGATAGCTACGTAATACAACTCGTTCTTATCTATTGATGAGTTCATGTTCAGCCTCCATTCAGATTGAGCTACTGCAAAGTGGCTTTAGCGGTCCCCTTTGCTGTCATTCCGTgc < 1:222351/136‑1 (MQ=255) gcagcaGTTGCAATTGCGATAGCTACGTAATACAACTCGTTCTTATCTATTGATGAGTTCATGTTCAGCCTCCATTCAGATTGAGCTACTGCAAAGTGGCTTTAGCGGTCCCCTTTGCTGTCATTCCGTGCGCGat < 1:480858/136‑1 (MQ=255) agcagTTGCAATTGCGATAGCTACGTAATACAACTCGTTCTTATCTATTGATGAGTTCATGTTCAGCCTCCATTCAGATTGAGCTACTGCAAAGTGGCTTTAGCGGTCCCCTTTGCTGTCATTCCGTGCGCGatat > 2:217448/1‑136 (MQ=255) tGCAATAGCGATAGCTACGTAATACATCTCGTTCTTATCTATTGATGAGTTCATGTTCAGCCTCCATTCAGATTGAGCTACTGCAAAGTGGCTTTAGCGGTCCCCTTTGCTGTCATTCCGTGCGCGATATTTGGaa < 2:316148/136‑1 (MQ=255) gTTCTTATCTATTGATGAGTTCATGTTCAGCCTCCATTCAGATTGAGCTACTGCAAAGTGGCTTTAGCGGTCCCCTTTGCTGTCATTCCGTGCGCGATATTGGGAATAGCTCCTCTGGATTGCGTGATTCAGAGtt > 1:59194/1‑136 (MQ=255) cTTATCTATTGATGAGTTCATGTTCAGCCTCCATTCAGATTGAGCTACTGCAAAGTGGCTTTAGCGGTCCCCTTTGCTGTCATTCCGTGCGCGATATTGGGAATAGCTCCTCTGGATTGCGTGATTCAGAGTTCAt > 2:14160/1‑136 (MQ=255) tATCTATTGATGAGTTCATGTTCAGCCTCCATTCAGATTGAGCTACTGCAAAGTGGCTTTAGCGGTCCCCTTTGCTGTCATTCCGTGCGCGATATTGGGAATAGCTCCTCTGGATTGCGTGATTCAGAGTTCAttt > 1:26749/1‑136 (MQ=255) aTGTTCAGCCTCCATTCAGATTGAGCTACTGCAAAGTGGCTTTAGCGGTCCCCTTTGCTGTCATTCCGTGCGCGATATTGGGAATAGCTCCTCTGGATTGCGTGATTCAGAGTTCATTTTTAAGCACTCCGCTTcc > 1:82710/1‑135 (MQ=255) tCAGCCTCCATTCAGATTGAGCTACTGCAAAGTGGCTTTAGCGGTCCCCTTTGCTGTCATTCCGTGCGCGATATTGGGAATAGCTCCTCTGGATTGCGTGATTCAGAGTTCATTTTTAAGCACTCCGCTTCACCTg < 1:191443/136‑1 (MQ=255) tCCATTCAGATTGAGCTACTGCAAAGTTGCTTTAGCGGTCCCCTTTGCTGTCATTCCGTGCGCGATATTGGGaa < 1:538901/74‑1 (MQ=255) tCCATTCAGATTGAGCTACTGCAAAGTTGCTTTAGCGGTCCCCTTTGCTGTCATTCCGTGCGCGATATTGGGaa < 1:536443/74‑1 (MQ=255) tCCATTCAGATTGAGCTACTGCAAAGTTGCTTTAGCGGTCCCCTTTGCTGTCATTCCGTGCGCGATATTGGGaa > 2:536443/1‑74 (MQ=255) tCCATTCAGATTGAGCTACTGCAAAGTTGCTTTAGCGGTCCCCTTTGCTGTCATTCCGTGCGCGATATTGGGaa > 2:538901/1‑74 (MQ=255) ttCAGATTGAGCTACTGCAAAGTGGCTTTAGCGGTCCCCTTTGCTGTCATTCCGTGCGCGATATTGGGAATAGCTCCTCTGGATTGCGTGATTCAGAGTTCATTTTTAAGCACTCCGCTTCACCTGCCGACCttgt < 1:65075/136‑1 (MQ=255) tGAGCTACTGCAAAGTGGCTTTAGCGGTCCCCTTTGCTGTCATTCCGTGCGCGATATTGGGAATAGCTCCTCTGGATTGCGTGATTCAGAGTTCATTTTTAAGCACTCCGCTTCACCTGCCGACCTTGTTGGGAAt > 2:433029/1‑136 (MQ=255) aaaGTGGCTTTAGCGGTCCCCTTTGCTGTCATTCCGTGCGCGATATTGGGAATAGCTCCTCTGGATTGCGTGATTCAGAGTTCATTTTTAAGCACTCCGCTTCACCTGCCGACCTTGTTGGGAATCCACGCTTGAg > 1:21731/1‑136 (MQ=255) gTGGCTTTAGCGGTCCCCTTTGCTGTCATTCCGTGCGCGATATTGGGAATAGCTCCTCTGGATTGCGTGATTCAGAGTTCATTTTTAAGCACTCCGCTTCACCTGCCGACCTTGTTGGGAATCCACGCTTGAGTCg < 2:59194/136‑1 (MQ=255) | CACCTCGGAAGCGTCAGTGGCCATCCAGATGAATAGGGCAGCAGTTGCAATTGCGATAGCTACGTAATACAACTCGTTCTTATCTATTGATGAGTTCATGTTCAGCCTCCATTCAGATTGAGCTACTGCAAAGTGGCTTTAGCGGTCCCCTTTGCTGTCATTCCGTGCGCGATATTGGGAATAGCTCCTCTGGATTGCGTGATTCAGAGTTCATTTTTAAGCACTCCGCTTCACCTGCCGACCTTGTTGGGAATCCACGCTTGAGTCG > NC_002947/1279365‑1279632 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |